Abstract:
:Regenerative medicine has emerged as an important discipline that aims to repair injury or replace damaged tissues or organs by introducing living cells or functioning tissues. Successful regenerative medicine strategies will likely depend upon a simultaneous optimization strategy for the design of biomaterials, cell-seeding methods, cell-biomaterial interactions, and molecular signaling within the engineered tissues. It remains a challenge to image three-dimensional (3-D) structures and functions of the cell-seeded scaffold in mesoscopic scale (>2 ∼ 3 mm). In this study, we utilized angled fluorescence laminar optical tomography (aFLOT), which allows depth-resolved molecular characterization of engineered tissues in 3-D to investigate cell viability, migration, and bone mineralization within bone tissue engineering scaffolds in situ.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Tang Q,Piard C,Lin J,Nan K,Guo T,Caccamese J,Fisher J,Chen Ydoi
10.1002/bit.26452subject
Has Abstractpub_date
2018-01-01 00:00:00pages
257-265issue
1eissn
0006-3592issn
1097-0290journal_volume
115pub_type
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