Recycle hollow fiber enzyme reactor with flow swing.

abstract：:Cellulose fibers in water form networks that give rise to an apparent yield stress, especially at high solids contents. Measuring the yield stress and correlating it with fiber concentration is important for the biomass and pulp industries. Understanding how the yield stress behaves at high solids concentrations is cr...

journal_title：Biotechnology and bioengineering

authors： Lavenson DM,Tozzi EJ,McCarthy MJ,Powell RL

更新日期：2011-10-01 00:00:00

abstract：:Tissue engineering devices as in vitro cell culture systems in scaffolds has encountered the bottleneck due to their much lower cell functions than real tissues/organs in vivo. Such situation has been improved in some extent by mimicking the cell microenvironments in vivo from either chemical or physical ways. However...

journal_title：Biotechnology and bioengineering

authors： Shen C,Meng Q,Zhang G

更新日期：2013-08-01 00:00:00

abstract：:The optimal of the specific growth rate was obtained with simple mathematical model in a yeast fed-batch cultures. The model was based on the mass balance around the fed-batch system and the relationship between the specific growth rate, mu, and the specific production rate of glutathione, rho(G). The optimal profile ...

journal_title：Biotechnology and bioengineering

authors： Shimizu H,Araki K,Shioya S,Suga K

更新日期：1991-06-20 00:00:00

abstract：:The fermentation kinetics of methane production from whey permeate in a packed bed immobilized cell bioreactor at mesophilic temperatures and pHs around neutral was studied. Propionate and acetate were the only two major organic intermediates found in the methanogenic fermentation of lactose. Based on this finding, a ...

journal_title：Biotechnology and bioengineering

authors： Yang ST,Quo M

更新日期：1991-02-20 00:00:00

abstract：:A kinetic resolution process for the production of chiral amines was developed using an enzyme-membrane reactor (EMR) and a hollow-fiber membrane contactor with (S)-specific omega-transaminases (omega-TA) from Vibrio fluvialis JS17 and Bacillus thuringiensis JS64. The substrate solution containing racemic amine and py...

journal_title：Biotechnology and bioengineering

authors： Shin JS,Kim BG,Liese A,Wandrey C

更新日期：2001-05-05 00:00:00

abstract：:Genetically-engineered cells offer a solution to the cell availability problem in tissue engineering a pancreatic substitute for the treatment of insulin-dependent diabetes. These cells can be non-beta cells, such as hepatocytes or myoblasts, retrieved as a biopsy from the same patient and genetically engineered to se...

journal_title：Biotechnology and bioengineering

authors： Cheng SY,Constantinidis I,Sambanis A

更新日期：2006-04-20 00:00:00

abstract：:Constitutive activation of Wnt signaling plays an important role in colorectal and liver tumorigenesis. Cell-based assays using synthetic TCF/LEF (T-cell factor/lymphoid enhancer factor) reporters, as readouts of β-catenin/TCF-dependent transcriptional activity, have contributed greatly to the discovery of small molec...

journal_title：Biotechnology and bioengineering

authors： Yamaguchi K,Zhu C,Ohsugi T,Yamaguchi Y,Ikenoue T,Furukawa Y

更新日期：2017-12-01 00:00:00

abstract：:Animal cell cultivation in vitro has been studied for more than 40 years. However, the culture medium composition has not been designed on the basis of the stoichiometric nutritional demands for animal cell growth. In this article, a model was developed to study the stoichiometric demands for nutrients (including gluc...

journal_title：Biotechnology and bioengineering

authors： Xie L,Wang DI

更新日期：1994-05-01 00:00:00

abstract：:Proper analysis of label distribution in metabolic pathway intermediates is critical for correct interpretation of experimental data and strategic experimental design. While, for example, 13C nuclear magnetic resonance (NMR) spectroscopy is usually limited to the measurement of degrees of 13C enrichment, more informat...

journal_title：Biotechnology and bioengineering

authors： Klapa MI,Park SM,Sinskey AJ,Stephanopoulos G

更新日期：1999-02-01 00:00:00

abstract：:Nanotechnology is becoming increasingly important for products used in our daily lives, such as the masses of titanium dioxide nanoparticle agglomerates (TiO(2) NPs) used in the pharmaceutical industry, for cosmetic products, or for pigments. Meanwhile, a serious lack of detailed information concerning the interaction...

journal_title：Biotechnology and bioengineering

authors： Chen P,Kanehira K,Sonezaki S,Taniguchi A

更新日期：2012-12-01 00:00:00

abstract：:A general framework for a genetically structured model is presented. The framework allows description of the interactions in a system of regulatory and structural genes. The model assumes equilibrium kinetics for the binding of regulatory proteins to the promoter regions of the genes and includes the possible activati...

journal_title：Biotechnology and bioengineering

authors： Agger T,Nielsen J

更新日期：1999-01-01 00:00:00

abstract：:A perfusion culture system was developed to investigate the oxygenation of high-density hybridoma cell cultures. The culture system was composed of a stirred-tank bioreactor and an external microfiltration hollow fiber cartridge for medium perfusion. Cell growth and antibody production were examined with large bubble ...

journal_title：Biotechnology and bioengineering

authors： Zhang S,Handa-Corrigan A,Spier RE

更新日期：1993-03-25 00:00:00

abstract：:Lectin-like function was demonstrated in this study for a novel water-soluble polymer with phenylboronic acid residues (poly (AAPBA-DMAm)), which induced appreciable proliferation of murine spleen lymphocytes with an increased expression of interleukin-2 (IL-2) receptor on their surface. Consequently, boosted prolifer...

journal_title：Biotechnology and bioengineering

authors： Uchimura E,Otsuka H,Okano T,Sakurai Y,Kataoka K

更新日期：2001-02-05 00:00:00

abstract：:Lignin, one of the major components of lignocellulosic biomass, plays an inhibitory role on the enzymatic hydrolysis of cellulose. This work examines the role of lignin in pretreated hardwood, where extents of cellulose hydrolysis decrease, rather than increase with increasing severity of liquid hot water pretreatment...

journal_title：Biotechnology and bioengineering

authors： Ko JK,Kim Y,Ximenes E,Ladisch MR

更新日期：2015-02-01 00:00:00

abstract：:Pseudomonas putida ATCC 17514 was used as a model strain to investigate the characteristics of bacterial growth in the presence of solid fluorene and phenanthrene. Despite the lower water-solubility of phenanthrene, P. putida degraded this polycyclic aromatic hydrocarbon (PAH) at a maximum observed rate of 1.4 +/- 0.1...

journal_title：Biotechnology and bioengineering

authors： Rodrigues AC,Wuertz S,Brito AG,Melo LF

更新日期：2005-05-05 00:00:00

abstract：:This study describes the development and application of a bioaffinity chromatographic system for the one-step purification of an NADP(+)-dependent secondary alcohol dehydrogenase from the obligate anaerobe, Thermoanaerobacter brockii (TBADH, EC 1.1.1.2). The general approach is based upon improving the selectivity of ...

journal_title：Biotechnology and bioengineering

authors： McMahon M,Mulcahy P

更新日期：2002-03-05 00:00:00

abstract：:Biofouling is the undesirable adhesion and development of microorganisms and macroorganisms in a water environment. An electrochemical antifouling system based on management of primary adhesion of microorganisms was developed employing titanium electrode for antifouling of seawater cooling pipes and marine infrastruct...

journal_title：Biotechnology and bioengineering

authors： Wake H,Takahashi H,Takimoto T,Takayanagi H,Ozawa K,Kadoi H,Okochi M,Matsunaga T

更新日期：2006-10-20 00:00:00

abstract：:The production of acetate by aerobically growing E. coli is examined. The problem is formulated in terms of a flow network that has as its objective maximal ATP synthesis. It is found that when loads are imposed and flux constraints exist either at the level of NADH turnover rate or the activity of a key Krebs cycle e...

journal_title：Biotechnology and bioengineering

authors： Majewski RA,Domach MM

更新日期：1990-03-25 00:00:00

abstract：:Ultrafiltration through Carbosep M(4) mineral membrane of protein solutions of decreasing complexity (whey before and after centrifugation or clarification, beta-lactoglobulin) was studied. Mathematical models were used to explain variations in flux with time. Taking into account variations in protein retention and hy...

journal_title：Biotechnology and bioengineering

authors： Taddei C,Daufin G,Aimar P,Sanchez V

更新日期：1989-06-20 00:00:00

abstract：:The biosynthesis of invertase by Saccharomyces carlsbergensis LAM 1068 was studied in relation to its glucose effect at both unsteady and steady states of growth. Experimental correlations between the dilution rate and invertase specific activity (E/X) in chemostat cultures led to an optimum for the enzyme synthesis a...

journal_title：Biotechnology and bioengineering

authors： Toda K

更新日期：1976-08-01 00:00:00

abstract：:The design and operation of an industrial penicillin-V deacylation reactor is simulated, using a kinetic expression and mass transport parameters for the immobilized enzyme particles which were determined experimentally in a previous study. It is desirable to use a series of equalsized plug flow reactors with pH contr...

journal_title：Biotechnology and bioengineering

authors： Karlsen LG,Villadsen J

更新日期：1984-12-01 00:00:00

abstract：:Protein switches have a variety of potential applications in biotechnology and medicine that motivate efforts to accelerate their development. Switches can be built by the proper fusion of two proteins with the prerequisite input and output functions. However, the exact fusion geometry for switch creation, which typic...

journal_title：Biotechnology and bioengineering

authors： Tullman J,Nicholes N,Dumont MR,Ribeiro LF,Ostermeier M

更新日期：2016-04-01 00:00:00

abstract：:Two thermophilic bacteria, Thermus aquaticus ATCC 25104 and Thermus species ATCC 27978, were investigated for their abilities to degrade BTEX (benzene, toluene, ethylbenzene, and xylenes). Thermus aquaticus and the Thermus sp. were grown in a nominal medium at 70 degrees C and 60 degrees C, respectively, and resting c...

journal_title：Biotechnology and bioengineering

authors： Chen CI,Taylor RT

更新日期：1995-12-20 00:00:00

abstract：:The study reports on the development of a bioreactor for the production of alpha-keto acids from D,L- or D-amino acids using Rhodotorula gracilis D-amino acid oxidase. D-amino acid oxidase was co-immobilized with catalase on Affi-Gel 10 matrix, and the reactor was operated as a continuous-stirred tank reactor (CSTR) o...

journal_title：Biotechnology and bioengineering

authors： Butò S,Pollegioni L,D'Angiuro L,Pilone MS

更新日期：1994-12-01 00:00:00

abstract：:A cybernetic framework is presented which views microbial response in multiple substrate environments as a judicious investment of cellular resources in synthesizing different key proteins according to an optimal regulatory strategy. A mathematical model is developed within the cybernetic framework for the diauxic gro...

journal_title：Biotechnology and bioengineering

authors： Dhurjati P,Ramkrishna D,Flickinger MC,Tsao GT

更新日期：1985-01-01 00:00:00

abstract：:A novel endoglucanase gene, celM , was cloned from a thermal spring metagenome. The gene was expressed in Escherichia coli, and the protein was extracted and purified. The protein catalyzed the hydrolysis of amorphous cellulose in a wide range of temperatures, 30-95°C, with optimal activity at 80°C. It was able to tol...

journal_title：Biotechnology and bioengineering

authors： Joshi N,Kaushal G,Singh SP

更新日期：2021-01-06 00:00:00

abstract：:Engineering protein expression in vitro or in vivo is usually straightforward for single genes, but remains challenging for multiple genes because of the requirement of coordinated control. RNA and protein overexpression strategies often exploit T7 RNA polymerase and its natural TPhi Class I terminator. However, this ...

journal_title：Biotechnology and bioengineering

authors： Du L,Gao R,Forster AC

更新日期：2009-12-15 00:00:00

abstract：:Various esters were synthesized in nearly anhydrous hexane from alcohols and carboxylic acids using a lipase from Candida cylindracea. The enzyme was immobilized on a nylon support and protein loadings as high as 10 mg/g were obtained. The activity of the immobilized enzyme was maximum in a range of temperatures from ...

journal_title：Biotechnology and bioengineering

authors： Carta G,Gainer JL,Benton AH

更新日期：1991-05-01 00:00:00

abstract：:An in vitro toxicity testing system that combines perfusion of a membrane-supported tissue model with measurement of trans-epithelial electrical resistance (TER) is described. The system is fully automated and allows for continuous TER measurements during exposure to a test agent. The utility of this system is demonst...

journal_title：Biotechnology and bioengineering

authors： Pasternak AS,Miller WM

更新日期：1996-06-05 00:00:00

abstract：:Esters were prepared by acylation of hydroxypropyl cellulose with fatty acid catalyzed by immobilized lipase from Candida antarctica in tert-butanol. The nature of the substrates used, the initial water activity of the system, and the molecular weight of the hydroxypropyl cellulose were investigated. Moreover, Fourier...

journal_title：Biotechnology and bioengineering

authors： Sereti V,Stamatis H,Pappas C,Polissiou M,Kolisis F

更新日期：2001-02-20 00:00:00