Abstract:
:Proper analysis of label distribution in metabolic pathway intermediates is critical for correct interpretation of experimental data and strategic experimental design. While, for example, 13C nuclear magnetic resonance (NMR) spectroscopy is usually limited to the measurement of degrees of 13C enrichment, more information about metabolic fluxes can be extracted from the fine structure of NMR spectra, or molecular weight distributions of isotopomers of metabolic intermediates (measured by gas chromatography-mass spectrometry). For this purpose, rigorous accounting for the contribution of all pathways to label distribution is required, especially contributions resulting from multiple turns of metabolic cycles. In this paper we present a mathematical model developed to analyze isotopomer distributions of tricarboxylic acid cycle (TCA) intermediates following the administration of 13C (or 14C) labeled substrates. The theory presented provides the basis to analyze 13C NMR spectra and molecular weight distributions of metabolites. In a companion paper (Park et al., 1999), the theory is applied to the analysis of several cases of biological significance. Copyright 1999 John Wiley & Sons, Inc.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Klapa MI,Park SM,Sinskey AJ,Stephanopoulos Gdoi
10.1002/(sici)1097-0290(19990220)62:4<375::aid-bitsubject
Has Abstractpub_date
1999-02-01 00:00:00pages
375-391issue
4eissn
0006-3592issn
1097-0290pii
10.1002/(SICI)1097-0290(19990220)62:4<375::AID-BITjournal_volume
62pub_type
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journal_title:Biotechnology and bioengineering
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