Optimal production of glutathione by controlling the specific growth rate of yeast in fed-batch culture.

Abstract:

:The optimal of the specific growth rate was obtained with simple mathematical model in a yeast fed-batch cultures. The model was based on the mass balance around the fed-batch system and the relationship between the specific growth rate, mu, and the specific production rate of glutathione, rho(G). The optimal profile of mu was calculated as a bang-bang type, That is mu, should start from the maximum value, mu(max) and should be kept at mu(max); then mu should be switched to mu(c), which gives a maximum value of rho(G). It was proven from the maximum principle that switching was needed only once, with the switching time from mu(max) to mu(c) depending on the final required glutathione content. Finally, this ideal profile of mu for the maximum production of glutathione was realized by manipulating the substrates feed rate in the fed-batch culture. Using the extended Kalman filter and a programmed-controller/feedback-compensator (PF) system, mu could be controlled at the optimal profile obtained. As a result, the maximum production of glutathione was accomplished fairly successfully. However, further improvement in the controller performance for mu is desired. The control strategy employed here can be applied to other batch reaction processes.

journal_name

Biotechnol Bioeng

authors

Shimizu H,Araki K,Shioya S,Suga K

doi

10.1002/bit.260380212

subject

Has Abstract

pub_date

1991-06-20 00:00:00

pages

196-205

issue

2

eissn

0006-3592

issn

1097-0290

journal_volume

38

pub_type

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