Abstract:
:Myristic acid esterification has been performed by an immobilized lipase from Mucor Miehei both in n-hexane and in supercritical carbon dioxide (SCCO(2)). The enzyme is stable in SCCO(2) at 15 MPa and 323 K. The reaction rate is influenced by the concentration of water and by the reaction medium composition. A reaction mechanism is proposed, and kinetic parameters are determined at 12.5 MPa and 313 K. Maximum velocity appears 1.5-fold higher in SCCO(2) than in n-hexane; however, as solubility of myristic acid is greater in n-hexane, it is not yet definitively clear that the supercritical medium is more favorable than the classical organic solvent for this type of enzyme reaction.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Dumont T,Barth D,Corbier C,Branlant G,Perrut Mdoi
10.1002/bit.260400218subject
Has Abstractpub_date
1992-06-20 00:00:00pages
329-33issue
2eissn
0006-3592issn
1097-0290journal_volume
40pub_type
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