Fluorophore-labeled carbohydrate analysis of immunoglobulin fusion proteins: Correlation of oligosaccharide content with in vivo clearance profile.

Abstract:

:CTLA4 is a membrane receptor on cytotoxic T cells whose interaction with the B7 counterreceptor on B cells is important in alloantigen responses. Soluble recombinant human and murine CTLA4 were produced using either Chinese hamster ovary or NS-0 cell lines. Expression vectors were constructed containing the gene coding for the extracellular domain of CTLA4 fused to either human lgG1 hinge, CH2, and CH3 domains or murine lgG2a hinge, CH2, and CH3 domain genes. These glycoproteins were produced in hollow-fiber or packed-bed-type bioreactors and purified from conditioned media by protein A affinity chromatography. Batches of purified CTLA4lg were analyzed for size, composition, and isoelectric point (pl) patterns by standard protein methods; oligosaccharide and monosaccharide profiles using several carbohydrate specific techniques; and in vivo clearance profiles using a murine model. Significant differences were observed between lots in their pl, clearance, and crbohydrate profiles. Higher overall pl values correlated with accelerated alpha-phase clearance and changes in oligosaccharide composition as determined by lectin binding analysis and electrophoresis of fluorophore-conjugated carbohydrates. Preparations exhibiting slower clearance profiles had oligosaccharides with higher quantities of N-acetylneuraminic acid and were predominantly of an N-linked biantennary complex-type. Conversely, batches with accelerated clearance profiles had less detectable N-acetylneuraminic acid. Oligosaccharides from murine CTLA4lg produced in NS-0 cells had terminal N-glycolylneuraminic acid but no detectable N-acetylneuraminic acid and had concomitant accelerated clearance. These data suggest that the presence and quantity of N-acetylneuraminic acid is an important component in predicting CTLA4lg plasma clearance rates and that production lots can be analyzed for oligosaccharide heterogeneity and sialic acid content by electrophoresis of fluorophore-conjugated carbohydrates. (c) 1995 John Wiley & Sons, Inc.

journal_name

Biotechnol Bioeng

authors

Flesher AR,Marzowski J,Wang WC,Raff HV

doi

10.1002/bit.260460502

subject

Has Abstract

pub_date

1995-06-05 00:00:00

pages

399-407

issue

5

eissn

0006-3592

issn

1097-0290

journal_volume

46

pub_type

杂志文章
  • A novel dielectrophoresis-based device for the selective retention of viable cells in cell culture media.

    abstract::Cost-effective production of biopharmaceuticals on a large scale can be carried out by perfusion cultures of mammalian cells. One problem with this mode of operation for submerged free-cell cultures is the requirement for an efficient cell separation device located in the effluent stream. The present work investigates...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0290(19970505)54:3<239::AID-BIT

    authors: Docoslis A,Kalogerakis N,Behie LA,Kaler KV

    更新日期:1997-05-05 00:00:00

  • Enzymatic glucosylation of hydrophobic alcohols in organic medium by the reverse hydrolysis reaction using almond-beta-D-glucosidase.

    abstract::Alkyl beta-D-glucosides were synthesized from D-glucose and alcohols by reverse hydrolysis using the commercially available almond beta-D-glucosidase in 9:1 (v/v) acetonitrile-water medium. The main characteristics of this enzyme-catalyzed glucosylation were established by using 2-hydroxybenzyl alcohol. The reaction i...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260460204

    authors: Vic G,Biton J,Le Beller D,Michel JM,Thomas D

    更新日期:1995-04-20 00:00:00

  • Lipase catalyzed esterification of glycidol in organic solvents.

    abstract::We studied the resolution of racemic glycidol through esterification with butyric acid catalyzed by porcine pancreatic lipase in organic media. A screening of seven solvents (log P values between 0.49 and 3.0, P being the n-octanol-water partition coefficient of the solvent) showed that neither log P nor the logarithm...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260420409

    authors: Martins JF,Da Ponte MN,Barreiros S

    更新日期:1993-08-05 00:00:00

  • The design of controllers for batch bioreactors.

    abstract::The implementation of control algorithms to batch bioreactors is often complicated by variations in process dynamics that occur during the course of fermentation. Such a wide operating range often renders the performance of fixed gain proportional-integral-differential (PID) controllers unsatisfactory. In this work, d...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260320415

    authors: Cardello RJ,San KY

    更新日期:1988-08-05 00:00:00

  • High-throughput determination of identity, purity, and quantity of combinatorial library members using LC/MS/UV/ELSD.

    abstract::We have used a high-throughput LC/MS/UV/ELSD method to rapidly determine the absolute quantity and purity of 42 organic compounds from seven lead discovery libraries. A general calibration curve generated from a different set of 42 compounds with seven different scaffolds was used in this analysis. We have also studie...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/1097-0290(2000)71:2<162::aid-bit1006>3.0.c

    authors: Fang L,Pan J,Yan B

    更新日期:2000-01-01 00:00:00

  • New FadB homologous enzymes and their use in enhanced biosynthesis of medium-chain-length polyhydroxyalkanoates in FadB mutant Escherichia coli.

    abstract::Recombinant Escherichia coli harboring the medium-chain-length (MCL) polyhydroxyalkanoate (PHA) synthase gene has been shown to accumulate MCL-PHAs from fatty acids when FadB is inactive. However, the enzymes in fadB mutant E. coli responsible for channeling the beta-oxidation intermediates to PHA biosynthesis have no...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20065

    authors: Park SJ,Yup Lee S

    更新日期:2004-06-20 00:00:00

  • Response dynamics of 26-, 34-, 39-, 54-, and 80-kDa proteases in induced cultures of recombinant Escherichia coli.

    abstract::Several researchers have demonstrated that the presence of a heterologous protein in recombinant Escherichia coli elicits a response similar to the heat-shock response, which includes enhanced protease expression. The present work detects, quantifies, and characterizes intracellular protease activity in E. coli that a...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260420602

    authors: Harcum SW,Bentley WE

    更新日期:1993-09-05 00:00:00

  • Attenuating apoptosis in Chinese hamster ovary cells for improved biopharmaceutical production.

    abstract::Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate l...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章,评审

    doi:10.1002/bit.27269

    authors: Henry MN,MacDonald MA,Orellana CA,Gray PP,Gillard M,Baker K,Nielsen LK,Marcellin E,Mahler S,Martínez VS

    更新日期:2020-04-01 00:00:00

  • Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains.

    abstract::A novel fed-batch approach for the production of L-phenylalanine (L-Phe) with recombinant E. coli is presented concerning the on-line control of the key fermentation parameters glucose and tyrosine. Two different production strains possessing either the tyrosine feedback resistant aroF(fbr) (encoding tyrosine feedback...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.10428

    authors: Gerigk M,Bujnicki R,Ganpo-Nkwenkwa E,Bongaerts J,Sprenger G,Takors R

    更新日期:2002-12-30 00:00:00

  • A novel recycle batch immobilized cell bioreactor for propionate production from whey lactose.

    abstract::Recycle batch fermentations using immobilized cells of Propionibacterium acidipropionici were studied for propionate production from whey permeate, de-lactose whey permeate, and acid whey. Cells were immobilized in a spirally wound fibrous sheet packed in a 0.5-L column reactor, which was connected to a 5-L stirred ta...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260450502

    authors: Yang ST,Huang Y,Hong G

    更新日期:1995-03-05 00:00:00

  • Adsorption of cellulase from Trichoderma reesei on cellulose and lignacious residue in wood pretreated by dilute sulfuric acid with explosive decompression.

    abstract::The adsorption of cellulase on cellulose and a lignacious residue was examined by using cellulase from Trichoderma reesei, hardwood pretreated by dilute sulfuric acid under high pressure, and a lignacious residue prepared by a complete enzymatic hydrolysis of the pretreated wood. A significant amount of cellulase was ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260360503

    authors: Ooshima H,Burns DS,Converse AO

    更新日期:1990-08-20 00:00:00

  • Understanding viral partitioning in two-phase aqueous nonionic micellar systems: 1. Role of attractive interactions between viruses and micelles.

    abstract::The partitioning behavior of viruses in the two-phase aqueous nonionic n-decyl tetra(ethylene oxide) (C10E4) micellar system cannot be fully explained by considering solely the repulsive, steric, excluded-volume interactions that operate between the viruses and the nonionic C10E4 micelles. Specifically, an excluded-vo...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.10193

    authors: Kamei DT,Liu CL,Haase-Pettingell C,King JA,Wang DI,Blankschtein D

    更新日期:2002-04-20 00:00:00

  • Effect of non-feeding period length on the intermittent operation of UASB reactors treating dairy effluents.

    abstract::Recent environmental concerns have prompted a re-evaluation of conventional management strategies and refueled the search of innovative waste management practices. In this sense, the anaerobic digestion of both fat and the remaining complex organic matter present in dairy wastewaters is attractive, although the contin...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.21095

    authors: Coelho NM,Rodrigues AA,Arroja LM,Capela IF

    更新日期:2007-02-01 00:00:00

  • Characterization of the diffusive properties of biofilms using pulsed field gradient-nuclear magnetic resonance

    abstract::The mobility of water in intact biofilms was measured with pulsed field gradient nuclear magnetic resonance (PFG-NMR) and used to characterise their diffusive properties. The results obtained with several well-defined systems, viz. pure water, agar, and agar containing inert particles or active bacteria were compared ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0290(19981105)60:3<283::aid-bit

    authors: Beuling EE,van Dusschoten D,Lens P,van den Heuvel JC,Van As H,Ottengraf SP

    更新日期:1998-11-05 00:00:00

  • Siderophore production by using free and immobilized cells of two pseudomonads cultivated in a medium enriched with Fe and/or toxic metals (Cr, Hg, Pb).

    abstract::Pseudomonads are serious candidates for siderophore production applied to toxic metal (TM) solubilization. The bioaugmentation of contaminated soils by these TM-solubilizing bacteria combined with phytoextraction is an emerging clean-up technology. Unfortunately, siderophore synthesis may be drastically reduced by sol...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20937

    authors: Braud A,Jézéquel K,Léger MA,Lebeau T

    更新日期:2006-08-20 00:00:00

  • Wine vinegar production using a noncommercial 100-litre bubble column reactor equipped with a novel type of dynamic sparger

    abstract::This paper describes batch and semicontinuous acetic acid fermentations for wine vinegar production carried out with Acetobacter pasteurianus, and an industrial strain using a noncommercial 100-L bubble column reactor equipped with a novel type of gas-liquid dynamic sparger. Results showed acetification rates with thi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0290(19990420)63:2<141::aid-bit

    authors: Fregapane G,Rubio-Fernandez H,Nieto J,Salvador MD

    更新日期:1999-04-20 00:00:00

  • Perturbation of syntrophic isobutyrate and butyrate degradation with formate and hydrogen.

    abstract::The effect of formate and hydrogen on isomerization and syntrophic degradation of butyrate and isobutyrate was investigated using a defined methanogenic culture, consisting of syntrophic isobutyrate-butyrate degrader strain IB, Methanobacterium formicicum strain T1N, and Methanosarcina mazeii strain T18. Formate and h...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0290(19961105)52:3<404::AID-BIT

    authors: Wu WM,Jain MK,Hickey RF,Zeikus JG

    更新日期:1996-11-05 00:00:00

  • Pilot scale recovery of recombinant annexin V from unclarified escherichia coli homogenate using expanded bed adsorption.

    abstract::Expanded bed adsorption is a new downstream processing technique forcapture of proteins directly from unclarified feedstocks. Expanded bed adsorption reduces the number of operations in purification processes by combining clarification, concentration, and capture into one operation. It is based on stable fluidization ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260440808

    authors: Barnfield Frej AK,Hjorth R,Hammarström A

    更新日期:1994-10-01 00:00:00

  • MODEL-molecular descriptor lab: a web-based server for computing structural and physicochemical features of compounds.

    abstract::Molecular descriptors represent structural and physicochemical features of compounds. They have been extensively used for developing statistical models, such as quantitative structure activity relationship (QSAR) and artificial neural networks (NN), for computer prediction of the pharmacodynamic, pharmacokinetic, or t...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.21214

    authors: Li ZR,Han LY,Xue Y,Yap CW,Li H,Jiang L,Chen YZ

    更新日期:2007-06-01 00:00:00

  • Evaluation of D-amino acid oxidase from Rhodotorula gracilis for the production of alpha-keto acids: a reactor system.

    abstract::The study reports on the development of a bioreactor for the production of alpha-keto acids from D,L- or D-amino acids using Rhodotorula gracilis D-amino acid oxidase. D-amino acid oxidase was co-immobilized with catalase on Affi-Gel 10 matrix, and the reactor was operated as a continuous-stirred tank reactor (CSTR) o...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260441104

    authors: Butò S,Pollegioni L,D'Angiuro L,Pilone MS

    更新日期:1994-12-01 00:00:00

  • A special reactor design for investigations of mixing time effects in a scaled-down industrial L-lysine fed-batch fermentation process

    abstract::A specially designed model reactor based on a 42-L laboratory fermentor was equipped with six stirrers (Rushton turbines) and five cylindrical disks. In this model reactor, the mixing time, Theta(90), turned out to be 13 times longer compared with the 42-L standard laboratory fermentor fitted with two Rushton turbines...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0290(19990905)64:5<599::aid-bit

    authors: Schilling BM,Pfefferle W,Bachmann B,Leuchtenberger W,Deckwer W

    更新日期:1999-09-05 00:00:00

  • Model-based derivation, analysis and control of unstable microaerobic steady-states--considering Rhodospirillum rubrum as an example.

    abstract::Microaerobic (oxygen-limited) conditions are critical for inducing many important microbial processes in industrial or environmental applications. At very low oxygen concentrations, however, the process performance often suffers from technical limitations. Available dissolved oxygen measurement techniques are not sens...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.25140

    authors: Carius L,Rumschinski P,Faulwasser T,Flockerzi D,Grammel H,Findeisen R

    更新日期:2014-04-01 00:00:00

  • Proposed mechanism of acetate accumulation in two recombinant Escherichia coli strains during high density fermentation.

    abstract::The productivity of Escherichia coli as a producer of recombinant proteins is affected by its metabolic properties, especially by acetate production. Two commercially used E. coli strains, BL21 (lambdaDE3) and JM109, differ significantly in their acetate production during batch fermentation at high initial glucose con...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0290(19980105)57:1<71::aid-bit9

    authors: van de Walle M,Shiloach J

    更新日期:1998-01-05 00:00:00

  • Effects of pH and acetic acid on homoacetic fermentation of lactate by Clostridium formicoaceticum.

    abstract::Clostridium formicoaceticum homofermentatively converts lactate to acetate at 37 degrees C and pH 6.6-9.6. However, this fermentation is strongly inhibited by acetic acid at acidic pH. The specific growth rate of this organism decreased from a maximum at pH 7.6 to zero at pH 6.6. This inhibition effect was found to be...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260340807

    authors: Tang IC,Okos MR,Yang ST

    更新日期:1989-10-20 00:00:00

  • Analysis of Golgi pH in Chinese hamster ovary cells using ratiometric pH-sensitive fluorescent proteins.

    abstract::Acidic Golgi pH plays an important role in protein glycosylation, one of the critical quality attributes of therapeutic proteins. To determine the intracellular Golgi pH during culture, stable Chinese hamster ovary (CHO) cell clones expressing pHluorin2, a ratiometric pH-sensitive fluorescent protein (FP), in the cis-...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.26920

    authors: Lee JH,Kim J,Park JH,Heo WD,Lee GM

    更新日期:2019-05-01 00:00:00

  • Effect of spatial architecture on cellular colonization.

    abstract::The spatial cell-material interaction remains vital issue in forming biodegradable scaffolds in Tissue Engineering. In this study, to understand the influence of spatial architecture on cellular behavior, 2D and 3D chitosan scaffolds of 50-190 kD and >310 kD MW were synthesized through air drying and controlled rate f...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20703

    authors: Huang Y,Siewe M,Madihally SV

    更新日期:2006-01-05 00:00:00

  • Cultivation of Methylosinus trichosporium OB3b: III. production of particulate methane monooxygenase in continuous culture.

    abstract::Continous culture experiments with the obligatory methanotroph, Methylosinus trichosporium OB3b, were conducted to study the whole-cell methane monooxygenase (MMO) and nitrogenase activities in a nitrate minimal salts medium under oxygen-limited conditions with methane as the carbone source. The important variables in...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.260400609

    authors: Shah NN,Park S,Taylor RT,Droege MW

    更新日期:1992-09-01 00:00:00

  • Effect of methanol feeding strategies on production and yield of recombinant mouse endostatin from Pichia pastoris.

    abstract::Pichia pastoris, a methylotrophic yeast, is an efficient producer of recombinant proteins in which the heterologous gene is under the control of the methanol-induced AOX1 promoter. Hence, the accepted production procedure has two phases: In the first phase, the yeast utilizes glycerol and biomass is accumulated; in th...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.10587

    authors: Trinh LB,Phue JN,Shiloach J

    更新日期:2003-05-20 00:00:00

  • Fluorene and phenanthrene uptake by Pseudomonas putida ATCC 17514: kinetics and physiological aspects.

    abstract::Pseudomonas putida ATCC 17514 was used as a model strain to investigate the characteristics of bacterial growth in the presence of solid fluorene and phenanthrene. Despite the lower water-solubility of phenanthrene, P. putida degraded this polycyclic aromatic hydrocarbon (PAH) at a maximum observed rate of 1.4 +/- 0.1...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.20377

    authors: Rodrigues AC,Wuertz S,Brito AG,Melo LF

    更新日期:2005-05-05 00:00:00

  • Selection and initial characterization of novel peptide ligands that bind specifically to human blood outgrowth endothelial cells.

    abstract::Using phage display technology, we have isolated 12-mer peptide ligands that bind to human blood outgrowth endothelial cells (HBOEC). To avoid non-specific binding we apply negative-positive selection approach by pre-incubating the library with non-HBOEC. The selected phage clones bind to their target cell population ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章

    doi:10.1002/bit.21420

    authors: Veleva AN,Cooper SL,Patterson C

    更新日期:2007-09-01 00:00:00