Abstract:
:It is shown that the mass transfer resistance can significantly distort the linearity of the Lineweaver-Burk plot of the kinetic data for a microbial culture which forms aggregates. For small flocs, the linearity of the Lineweaver-Burk plot is largely retained, but a different slope and intercept will be obtained compared with flocs free from mass transfer resistance. For large flocs, the Lineweaver-Burk plot shows pronounced curvature at high limiting substrate concentrations. Hence, if the true intrinsic kinetic parameters are to be extracted from a highly flocculating microbial culture, sufficient agitation has to be provided to remove the effect of mass transfer resistance. If the behavior of the flocculating microbial culture is to be explored, additional values for some physical parameters, such as the effective diffusion coefficient of the substrate in floc, the floc density, and the mean floc radius, are needed.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Ngian KF,Lin SH,Martin WRdoi
10.1002/bit.260191204subject
Has Abstractpub_date
1977-12-01 00:00:00pages
1773-84issue
12eissn
0006-3592issn
1097-0290journal_volume
19pub_type
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journal_title:Biotechnology and bioengineering
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