Abstract:
:Modification of cellular functions by overexpression of genes is being increasingly practiced for tissue engineering. In the present study, we investigated whether transfection efficiency could be enhanced by magnetofection that involves the use of plasmid DNA (pDNA)/magnetite cationic liposomes (MCLs) complexes (pDNA/MCL) and magnetic force. The transfection efficiencies of the magnetofection technique by pDNA/MCL in fibroblasts and keratinocytes using reporter genes were 36- and 10-fold higher, respectively, than those of a lipofection technique by cationic liposomes. Moreover, in vitro construction of three-dimensional (3D) tissues is an important challenge. We recently proposed a novel technique termed "magnetic force-based tissue engineering" (Mag-TE) to produce 3D tissues. Since the fibroblasts after magnetofection incorporated both magnetite nanoparticles and pDNA, we investigated whether multilayered heterotypic cell sheets expressing transgene could be fabricated by Mag-TE. First, the fibroblasts were seeded onto an ultra-low attachment culture plate. When a magnet was placed under the plate, the cells accumulated at the bottom of the culture plate. After 24 h of culture, the transgene-expressing cells formed a multilayered cell sheet-like structure. These results indicated that MCLs are a potent biomanipulation tool for both gene transfer and 3D tissue construction, suggesting that these techniques are useful for tissue engineering.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Ino K,Kawasumi T,Ito A,Honda Hdoi
10.1002/bit.21738subject
Has Abstractpub_date
2008-05-01 00:00:00pages
168-76issue
1eissn
0006-3592issn
1097-0290journal_volume
100pub_type
杂志文章abstract::Recently it has been established that low molecular weight displacers can be successfully employed for the purification of proteins in hydrophobic interaction chromatography (HIC) systems. This work investigates the utility of this technique for the purification of an industrial protein mixture. The study involved the...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10582
更新日期:2003-05-05 00:00:00
abstract::Screening fifteen strains of white-rot fungi for their ability to decolorize combined bleached kraft effluent showed that Coriolus versicolor in liquid culture removed over 60% of the color of the effluent within six days in the presence of sucrose. Treatment of the same effluent with this fungus, immobilized in beads...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260250814
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260330908
更新日期:1989-04-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24713
更新日期:2013-02-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20130
更新日期:2004-07-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260381016
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journal_title:Biotechnology and bioengineering
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doi:10.1002/bit.10079
更新日期:2002-03-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21546
更新日期:2008-02-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260190705
更新日期:1977-07-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21796
更新日期:2008-07-01 00:00:00
abstract::Based on batch cultivation data for the production of L-glutamic acid from glucose, a comparative evaluation was made for repeated batch cultivations using one and more fermentors. The problem was formulated as maximizing the productivity of metabolic product with the specified conversion with respect to the cell age ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270524
更新日期:1985-05-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21492
更新日期:2007-12-01 00:00:00
abstract::Biosensors with animal and microbial cells immobilized close to the tip of a membrane electrode have been developed for chemical and drug testing. Our experimental results show that biosensors can be used for drug screening and to provide useful information about various cell-chemical interactions. A computer aided an...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260310311
更新日期:1988-02-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960920)51:6<725::AID-BIT
更新日期:1996-09-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22521
更新日期:2010-01-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19970420)54:2<122::AID-BIT
更新日期:1997-04-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260480412
更新日期:1995-11-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260250610
更新日期:1983-06-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960505)50:3<329::AID-BIT
更新日期:1996-05-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280312
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390405
更新日期:1992-02-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260340703
更新日期:1989-10-05 00:00:00
abstract::Ralstonia eutropha grows on and produces polyhydroxyalkanoates (PHAs) from fermentation acids. Acetic acid, one major organic acid from acidogenesis of organic wastes, has an inhibitory effect on the bacterium at slightly alkaline pH (6 g HAc/L at pH 8). The tolerance of R. eutropha to acetate, however, was increased ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1080
更新日期:2001-06-20 00:00:00
abstract::Expression of the lactose (lac) operon in the Escherichia coli chromosome has been studied in mixed-sugar chemostat cultures under steady-state and transient conditions. A unified model has been formulated which involves regulation of active inducer (lactose) transport, promoter-operator regulated expression of the la...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260290812
更新日期:1987-06-01 00:00:00
abstract::The kinetics of the cellulase-catalyzed conversion of soluble cellulose into glucose have been studied over a range of substrate concentrations and temperatures, and at pH values ranging from 4.75 to 7.0. Lineweaver-Burk plots were linear and led to V = 6.2muM/s and K(m) = 13.1 mM at pH 5.8 and 25.0 degrees C. The pK ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260251011
更新日期:1983-10-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260370914
更新日期:1991-04-15 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章,评审
doi:
更新日期:1998-04-20 00:00:00
abstract::Biosorption of elements is a little understood phenomenon exhibited by some types of even nonliving microbial biomass. A common fungus Rhizopus arrhizus has been reported to take up uranium from aqueous solutions to the extent of 180 mg U(6+)/g. The mechanism of uranium sequestering by this type of biomass was studied...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260240211
更新日期:1982-02-01 00:00:00