Abstract:
:Fed-batch cultures of recombinant microorganisms have attracted attention as they can separate cell growth stage from cloned-gene expression phase during fermentations. In this work, the effect of different glucose feeding strategies on cell growth and cloned gene expression was studied during aerobic fed-batch fermentations of recombinant yeast, containing the plasmid pRB58. The plasmid contains the yeast SUC2 gene, which codes for the enzyme invertase. Some feeding policies resulted in a constant glucose concentration inside the fermentor, while others deliberately introduced a cyclic variation. The cell mass yield was found to be higher at low glucose concentrations, thus indicating a shift to the more energy-efficient respiratory pathway. The SUC2 gene expression was derepressed at glucose levels below 2 g/L. The response of specific invertase activity to changes in the medium glucose concentration was found to be almost immediate.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Patkar A,Seo JHdoi
10.1002/bit.260400115subject
Has Abstractpub_date
1992-06-05 00:00:00pages
103-9issue
1eissn
0006-3592issn
1097-0290journal_volume
40pub_type
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