Non-carbon loss long-term continuous lactic acid production from mixed sugars using thermophilic Enterococcus faecium QU 50.

abstract：:In our preceding article, we demonstrated a procedure based upon enzymic flow microcalorimetry using an enzyme thermistor (ET) to characterize the microkinetic properties of an immobilized enzyme (IME) and its further application in the screening of IMEs. To consider the ET method (single ET unit, ET system 1) as stan...

journal_title：Biotechnology and bioengineering

authors： Gemeiner P,Docolomansky P,Nahálka J,Stefuca V,Danielsson B

更新日期：1996-01-05 00:00:00

abstract：:Catalytic membranes, obtained by immobilizing thermophilic beta-glycosidase onto nylon supports, were used in a nonisothermal bioreactor to study the effect of temperature gradients on the rate of enzyme reaction. Two experimental approaches were carried out to explain the molecular mechanisms by which the temperature...

journal_title：Biotechnology and bioengineering

authors： Febbraio F,Portaccio M,Stellato S,Rossi S,Bencivenga U,Nucci R,Rossi M,Gaeta FS,Mita DG

更新日期：1998-07-05 00:00:00

abstract：:Rate and yield expressions relating to biomass and xanthan formation and to nitrogen, glucose, and oxygen consumption were established for Xanthomonas campestris batch fermentations in a bubble column. Microbial growth was described by the logistic rate equation, characterized by a maximum specific growth rate mu(M) =...

journal_title：Biotechnology and bioengineering

authors： Pons A,Dussap CG,Gros JB

更新日期：1989-01-20 00:00:00

abstract：:All bacteria release organic compounds called soluble microbial products (SMP) as a part of their normal metabolism. In photobioreactor (PBR) settings, SMP produced by cyanobacteria represent a major pool of carbon and electrons available to heterotrophic bacteria. Thus, SMP in PBRs are a major driver for the growth o...

journal_title：Biotechnology and bioengineering

authors： Zevin AS,Nam T,Rittmann B,Krajmalnik-Brown R

更新日期：2015-09-01 00:00:00

abstract：:Self-cycling fermentation (SCF), a cyclical, semi-continuous process that induces cell synchrony, was incorporated into a recombinant protein production scheme. Escherichia coli CY15050, a lac(-) mutant lysogenized with temperature-sensitive phage λ modified to over-express β-galactosidase, was used as a model system....

journal_title：Biotechnology and bioengineering

authors： Storms ZJ,Brown T,Sauvageau D,Cooper DG

更新日期：2012-09-01 00:00:00

abstract：:A novel reaction system was developed to maximize the catalytic efficiency of chloroperoxidase (CPO, from Caldariomyces fumago) toward the oxidation of hydrocarbons. The reaction system consisted of an organic/aqueous emulsion comprising pure substrate and aqueous buffer supplemented with the surfactant dioctyl sulfos...

journal_title：Biotechnology and bioengineering

authors： Park JB,Clark DS

更新日期：2006-05-05 00:00:00

abstract：:Oil residues arising from the Christos-Bitas spillage were found to contain 28% of oil extractable by carbon tetrachloride; the remainder consisted of water and undefined solids. Christos-Bitas mousse was added to 1.18 m(3) liquor inoculated with oil-contaminated marine mud, and aerated with a 1.5-hp vortex pump and v...

journal_title：Biotechnology and bioengineering

authors： Berwick PG

更新日期：1985-01-01 00:00:00

abstract：:The methanol-regulated alcohol oxidase promoter (PAOX1 ) of Pichia pastoris (syn. Komagataella spp. ) is one of the strongest promoters for heterologous gene expression. Although increasing the gene dosage is a common strategy to improve recombinant protein productivities, P. pastoris strains harboring more than two c...

journal_title：Biotechnology and bioengineering

authors： Cámara E,Monforte S,Albiol J,Ferrer P

更新日期：2019-07-01 00:00:00

abstract：:After separation by microscale non-denaturing two-dimensional gel electrophoresis (2DE) and transferring to a blotting membrane, major proteins are detected by a staining of direct blue 71 in a neutral solution. The carboxylesterase on the membrane hydrolyzes phosphatidylcholine after the spot of carboxylesterase is e...

journal_title：Biotechnology and bioengineering

authors： Shimazaki Y,Miyamoto M

更新日期：2007-11-01 00:00:00

abstract：:In this work, novel conductive composite nanofiber mesh possessing reactive groups was electrospun from solutions containing poly(acrylonitrile-co-acrylic acid) (PANCAA) and multi-walled carbon nanotubes (MWCNTs) for redoxase immobilization, assuming that the incorporated MWCNTs could behave as electrons transferor du...

journal_title：Biotechnology and bioengineering

authors： Wang ZG,Ke BB,Xu ZK

更新日期：2007-07-01 00:00:00

abstract：:Trichoderma reesei (QM 9414) produced cellulase in continuous culture, on media containing xylose (1%) supplemented with sorbose (0.3%) to induce cellulase production. Maximum cell mass of 4.54 kg/m(3) occurred at pH 4.0 and a dilution rate of 0.0391 h(-1) where residual substrate was 0.43 kg/m(3), but no cellulase wa...

journal_title：Biotechnology and bioengineering

authors： Schafner DW,Toledo RT

更新日期：1992-04-05 00:00:00

abstract：:Olive oil hydrolysis using Chromobacterium viscosum lipase B in a reversed micellar media was investigated in a membrane reactor. The dynamic evolution of the product concentration both in the concentrate and permeate stream was analyzed using a mechanistic model previously developed by us and further modified in this...

journal_title：Biotechnology and bioengineering

authors： Prazeres DM,Lemos F,Garcia FA,Cabral JM

更新日期：1993-09-05 00:00:00

abstract：:Escherichia coli has been the host organism most frequently investigated for efficient recombinant protein production. However, the production of a foreign protein in recombinant E. coli often leads to growth deterioration and elevated secretion of acetic acid. Such observed phenomena have been widely linked with cell...

journal_title：Biotechnology and bioengineering

authors： Zeng H,Yang A

更新日期：2019-06-01 00:00:00

abstract：:Phage Φ80 can infect Escherichia coli in a stealthy manner and persist by forming lysogens. Such Φ80 lysogens are fairly common and often go undetected unless the host is grown at temperatures below 37°C. Since low growth temperatures are required for growing temperature-sensitive mutants and often preferred for large...

journal_title：Biotechnology and bioengineering

authors： Srinivas S,Cronan JE

更新日期：2019-07-01 00:00:00

abstract：:A nuclear magnetic resonance (NMR) method has been developed to monitor on-line lipase-catalyzed esterification reactions without the need to sample the reaction medium. The technique, through (1)H NMR, measures the concentrations of alcohol, ester, hydroxylic hydrogens in the organic phase, and hydroxylic hydrogens i...

journal_title：Biotechnology and bioengineering

authors： Sarazin C,Ergan F,Séguin JP,Goethals G,Legoy MD,Barbotin JN

更新日期：1996-09-20 00:00:00

abstract：:Breast cancer cells experience a range of shear stresses in the tumor microenvironment (TME). However most current in vitro three-dimensional (3D) models fail to systematically probe the effects of this biophysical stimuli on cancer cell metastasis, proliferation, and chemoresistance. To investigate the roles of shear...

journal_title：Biotechnology and bioengineering

authors： Novak CM,Horst EN,Taylor CC,Liu CZ,Mehta G

更新日期：2019-11-01 00:00:00

abstract：:Pseudomonas putida ATCC 17514 was used as a model strain to investigate the characteristics of bacterial growth in the presence of solid fluorene and phenanthrene. Despite the lower water-solubility of phenanthrene, P. putida degraded this polycyclic aromatic hydrocarbon (PAH) at a maximum observed rate of 1.4 +/- 0.1...

journal_title：Biotechnology and bioengineering

authors： Rodrigues AC,Wuertz S,Brito AG,Melo LF

更新日期：2005-05-05 00:00:00

abstract：:Production of conidia of the biocontrol fungus Coniothyrium minitans by solid-state cultivation in a packed-bed reactor on an industrial scale is feasible. Spore yield and oxygen consumption rate of C. minitans during cultivation on oats and three inert solids (hemp, perlite, and bagasse) saturated with a liquid mediu...

journal_title：Biotechnology and bioengineering

authors： Weber FJ,Tramper J,Rinzema A

更新日期：1999-11-20 00:00:00

abstract：:Lipase from Pseudomonas fluorescens biotype I was immobilized by adsorption of anion exchange resin using glutaraldehyde to enhance the adsorption. The activity yield of the immobilized lipase was very low (below 1%) when lipase activity was measured using emulsion substrate. The activity yield was 10-70% when lipase ...

journal_title：Biotechnology and bioengineering

authors： Kosugi Y,Tanaka H,Tomizuka N

更新日期：1990-09-01 00:00:00

abstract：:We Propose a kinetic expression which accounts for the temperature dependence of ethanol yield losses in batch alcoholic fermentation. Moreover, the characteristic parameters of the microbial growth equation have been calculated for Saccharomyces cerevisiae under typical wine industry conditions. A substrate consumpti...

journal_title：Biotechnology and bioengineering

authors： Caro I,Pérez L,Cantero D

更新日期：1991-10-05 00:00:00

abstract：:A means of rapidly renaturing denatured protein was devised and evaluated. Three liquids were laminarly layered in a centrifuge tube, in which two solutions sandwiched liquid paraffin so as to form a pseudolipid bilayer. Denatured and aggregted protein placed on the upper surface of liquid paraffin was renatured as it...

journal_title：Biotechnology and bioengineering

authors： Yoshii H,Kojima T,Furuta T,Yasunishi A

更新日期：1994-01-05 00:00:00

abstract：:The thermo-responsive polymer poly(N-isopropylacrylamide) has received widespread attention for its in vitro application in the non-invasive, non-destructive release of adherent cells on two dimensional surfaces. In this study, 3D non-woven scaffolds fabricated from poly(propylene) (PP), poly(ethylene terephthalate) (...

journal_title：Biotechnology and bioengineering

authors： Rossouw CL,Chetty A,Moolman FS,Birkholtz LM,Hoppe H,Mancama DT

更新日期：2012-08-01 00:00:00

abstract：:Green fluorescent protein (GFP) was used to study the regulation of the galactose-inducible GAL1 promoter in yeast Saccharomyces cerevisiae strains. GFP was cloned into the pGAL110 vector and transformed into the yeast strains. Time course studies comparing culture fluorescence intensity and GFP concentration were con...

journal_title：Biotechnology and bioengineering

authors： Li J,Wang S,VanDusen WJ,Schultz LD,George HA,Herber WK,Chae HJ,Bentley WE,Rao G

更新日期：2000-10-20 00:00:00

abstract：:An in vitro toxicity testing system that combines perfusion of a membrane-supported tissue model with measurement of trans-epithelial electrical resistance (TER) is described. The system is fully automated and allows for continuous TER measurements during exposure to a test agent. The utility of this system is demonst...

journal_title：Biotechnology and bioengineering

authors： Pasternak AS,Miller WM

更新日期：1996-06-05 00:00:00

abstract：:The treatment of meta- and para-nitrobenzoic acid in an industrial wastestream by Comamonas sp. JS46 and Comamonas sp. JS47 is investigated. The most important feature of the wastestream is the constantly changing concentration ratio of the two isomers. The most extreme occurrence is considered here: the complete chan...

journal_title：Biotechnology and bioengineering

authors： Goodall JL,Thomas SM,Spain JC,Peretti SW

更新日期：1998-07-05 00:00:00

abstract：:Mathematical modeling of animal cell growth and metabolism is essential for the understanding and improvement of the production of biopharmaceuticals. Models can explain the dynamic behavior of cell growth and product formation, support the identification of the most relevant parameters for process design, and signifi...

journal_title：Biotechnology and bioengineering

authors： Ramos JRC,Rath AG,Genzel Y,Sandig V,Reichl U

更新日期：2020-05-01 00:00:00

abstract：:Animal cell culture is widely used in biotechnology for the production of many biological products. In situ microscopes acquire images directly from cell suspensions and analyze the images in matters of cell concentration, cell size distribution and cell morphology. Their applicability was already proven for yeast and...

journal_title：Biotechnology and bioengineering

authors： Rudolph G,Lindner P,Gierse A,Bluma A,Martinez G,Hitzmann B,Scheper T

更新日期：2008-01-01 00:00:00

abstract：:Mixed-culture fermentation is a key central process to enable next generation biofuels and biocommodity production due to economic and process advantages over application of pure cultures. However, a key limitation to the application of mixed-culture fermentation is predicting culture product response, related to meta...

journal_title：Biotechnology and bioengineering

authors： Hoelzle RD,Virdis B,Batstone DJ

更新日期：2014-11-01 00:00:00

abstract：:Model experiments were performed with starved yeast (Saccharomyces cerevisiae) cultures in a batch reactor in order to develop a better understanding of NAD(P)H and flavin culture fluorescence. Fluorescence was monitored during aerobic-anaerobic-aerobic transitions and ethanol and glucose substrate addition experiment...

journal_title：Biotechnology and bioengineering

authors： Siano SA,Mutharasan R

更新日期：1989-08-20 00:00:00

abstract：:Stem cell self-renewal versus differentiation fate decisions are difficult to characterize and analyze due to multiple competing rate processes occurring simultaneously among heterogeneous cell subpopulations. To address this challenge, we describe a mathematical model for cell population dynamics that allows flow cyt...

journal_title：Biotechnology and bioengineering

authors： Prudhomme WA,Duggar KH,Lauffenburger DA

更新日期：2004-11-05 00:00:00