Abstract:
:Lipase from Pseudomonas fluorescens biotype I was immobilized by adsorption of anion exchange resin using glutaraldehyde to enhance the adsorption. The activity yield of the immobilized lipase was very low (below 1%) when lipase activity was measured using emulsion substrate. The activity yield was 10-70% when lipase activity was measured using non-emulsion substrate. Countercurrent reactors for hydrolysis of oil using non-emulsion substrate were studied. A fluidized bed reactor was found to be superior to a fixed bed one since in a fixed bed reactor the separation rate of the two layers was slow and the flow rate of the reactor had to be slower than the separation rate. A fluidized bed reactor system equipped with settling compartments and stirring compartments was devised. Continuous lipolysis at 60 degrees C and continuous separation of oily product and water soluble product were performed. After continuous operation for more than 3 months, 70% of the initial activity of the immobilized lipase was observed at the end of the reaction.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Kosugi Y,Tanaka H,Tomizuka Ndoi
10.1002/bit.260360609subject
Has Abstractpub_date
1990-09-01 00:00:00pages
617-22issue
6eissn
0006-3592issn
1097-0290journal_volume
36pub_type
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