Fluorene and phenanthrene uptake by Pseudomonas putida ATCC 17514: kinetics and physiological aspects.

abstract：:It is well established that salt enhances the interaction between solutes (e.g., proteins, displacers) and the weak hydrophobic ligands in hydrophobic interaction chromatography (HIC) and that various salts (e.g., kosmotropes, chaotropes, and neutral) have different effects on protein retention. In this article, the s...

journal_title：Biotechnology and bioengineering

authors： Xia F,Nagrath D,Garde S,Cramer SM

更新日期：2004-08-05 00:00:00

abstract：:A model was formulated to examine specific experimental data of growth and heterologous product formation with recombinant Saccharomyces cerevisiae while incorporating available literature. The model simulated dry cell weight, glucose, ethanol, dissolved oxygen, human Epidermal Growth Factor (hEGF) production, fractio...

journal_title：Biotechnology and bioengineering

authors： Coppella SJ,Dhurjati P

更新日期：1990-02-20 00:00:00

abstract：:Steady-state simulations using our previously developed structured kinetic model of antibody synthesis and secretion by hybridoma cells are used here in conjunction with factorial design analysis to identify intracellular parameters important in determining the specific antibody secretion rate and predict the dependen...

journal_title：Biotechnology and bioengineering

authors： Bibila TA,Flickinger MC

更新日期：1992-02-05 00:00:00

abstract：:Hydrocarbon oils of the alga Botryococcus braunii, extracted from a natural "bloom" of the plant, have been hydrocracked to produce a distillate comprising 67% gasoline fraction, 15% aviation turbine fuel fraction, 15% diesel fuel fraction, and 3% residual oil. The distillate was examined by a number of standard petro...

journal_title：Biotechnology and bioengineering

authors： Hillen LW,Pollard G,Wake LV,White N

更新日期：1982-01-01 00:00:00

abstract：:The effector functions elicited by IgG antibodies strongly depend on the carbohydrate moiety linked to the Fc region of the protein. Therefore several approaches have been developed to rationally manipulate these glycans and improve the biological functions of the antibody. Overexpression of recombinant beta1,4-N-acet...

journal_title：Biotechnology and bioengineering

authors： Ferrara C,Brünker P,Suter T,Moser S,Püntener U,Umaña P

更新日期：2006-04-05 00:00:00

abstract：:A dynamic method is proposed for k(l)a measurement in aerated and agitated reactors, in which a change in the total pressure in the reactor by approximately 20% leads to a simultaneous change in the oxygen concentration in all the bubbles in the dispersion. This procedure suppresses the influence of nonideal mixing of...

journal_title：Biotechnology and bioengineering

authors： Linek V,Benes P,Vacek V

更新日期：1989-05-01 00:00:00

abstract：:A gene for ethionine resistance isolated from the yeast Saccharomyces cerevisiae DKD-5D-H conferred on the yeast cells resistance to seleno-L-methionine and capability to produce S-adenosyl-L-methionine in the cells. An enzymatic study of the L-methionine synthetic pathway of L-methionine proto- and auxotrophs and in ...

journal_title：Biotechnology and bioengineering

authors： Shiomi N,Fukuda H,Fukuda Y,Murata K,Kimura A

更新日期：1990-05-01 00:00:00

abstract：:Protein phosphorylation is an important post-translational modification for intracellular signaling molecules, mostly found in serine and threonine residues. Tyrosine phosphorylations are very few events (less than 0.1% to phosphorylated serine/threonine residues), but capable of governing cell fate decisions involved...

journal_title：Biotechnology and bioengineering

authors： Yoshimoto N,Ikeda Y,Tatematsu K,Iijima M,Nakai T,Okajima T,Tanizawa K,Kuroda S

更新日期：2016-08-01 00:00:00

abstract：:Natural oxygen gradients occur in tissues of biological organisms and also in the context of three-dimensional (3D) in vitro cultivation. Oxygen diffusion limitation and metabolic oxygen consumption by embedded cells produce areas of hypoxia in the tissue/matrix. However, reliable systems to detect oxygen gradients an...

journal_title：Biotechnology and bioengineering

authors： Schmitz C,Pepelanova I,Seliktar D,Potekhina E,Belousov VV,Scheper T,Lavrentieva A

更新日期：2020-07-15 00:00:00

abstract：:The effect of trehalose (0.5 M) on the thermal stability of cutinase in the alkaline pH range was studied. The thermal unfolding induced by increasing temperature was analyzed in the absence and in the presence of trehalose according to a two-state model (which assumes that only the folded and unfolded states of cutin...

journal_title：Biotechnology and bioengineering

authors： Baptista RP,Cabral JM,Melo EP

更新日期：2000-12-20 00:00:00

abstract：:We show the design features of a membrane bioreactor based on pulsatile flow across dimpled membranes. Results show an enhanced mass transfer of air of at least five-fold magnitude as compared with flat membranes. An increased working volume form 20 mL to 120 mL reduced the k(L)A at a given Reynolds number because of ...

journal_title：Biotechnology and bioengineering

authors： Beeton S,Millward HR,Bellhouse BJ,Nicholson AM,Jenkins N,Knowles CJ

更新日期：1991-12-05 00:00:00

abstract：:As the therapeutic monoclonal antibody (mAb) market continues to grow, optimizing production processes is becoming more critical in improving efficiencies and reducing cost-of-goods in large-scale production. With the recent trends of increasing cell culture titers from upstream process improvements, downstream capaci...

journal_title：Biotechnology and bioengineering

authors： Dizon-Maspat J,Bourret J,D'Agostini A,Li F

更新日期：2012-04-01 00:00:00

abstract：:A nuclear magnetic resonance (NMR) method has been developed to monitor on-line lipase-catalyzed esterification reactions without the need to sample the reaction medium. The technique, through (1)H NMR, measures the concentrations of alcohol, ester, hydroxylic hydrogens in the organic phase, and hydroxylic hydrogens i...

journal_title：Biotechnology and bioengineering

authors： Sarazin C,Ergan F,Séguin JP,Goethals G,Legoy MD,Barbotin JN

更新日期：1996-09-20 00:00:00

abstract：:A protein loaded three-dimensional scaffold can be used for protein delivery and bone tissue regeneration. The main objective of this project was to develop recombinant human bone morphogenetic protein-2 (rhBMP-2) loaded poly(D,L-lactide-co-glycolide)/hydroxylapatite (PLGA/HAp) composite fibrous scaffolds through a pr...

journal_title：Biotechnology and bioengineering

authors： Nie H,Soh BW,Fu YC,Wang CH

更新日期：2008-01-01 00:00:00

abstract：:In this study it is reported the operation of a horizontal-flow anaerobic immobilized biomass (HAIB) reactor under sulfate-reducing condition which was also exposed to different amounts of ethanol and toluene. The system was inoculated with sludge taken from up-flow anaerobic sludge blanket (UASB) reactors treating re...

journal_title：Biotechnology and bioengineering

authors： Cattony EB,Chinalia FA,Ribeiro R,Zaiat M,Foresti E,Varesche MB

更新日期：2005-07-20 00:00:00

abstract：:Increases in our understanding of gene function have greatly expanded the repertoire of possible genetic interventions at our disposal with the consequence that many genetic engineering applications require multiple manipulations in which target genes can be both overexpressed and silenced in a simple and co-ordinated...

journal_title：Biotechnology and bioengineering

authors： Greber D,Fussenegger M

更新日期：2007-04-01 00:00:00

abstract：:To widen the biological control function of a genetically modified Bacillus thuringiensis subsp leesis strain BMB-005, an acyl homoserine lactonase (AHL lactonase) gene aiiA transcribed by the promoter of insecticidal crystal protein coding gene cry3A, was transformed into strain BMB-005. The amount of AHL lactonase p...

journal_title：Biotechnology and bioengineering

authors： Zhu C,Yu Z,Sun M

更新日期：2006-10-20 00:00:00

abstract：:Pretreatment of rice straw by using renewable cholinium amino acids ionic liquids ([Ch][AA] ILs)-water mixtures and the subsequent enzymatic hydrolysis of the residues were conducted in the present work. Of the eight mixtures composed of ILs and water, most were found to be effective for rice straw pretreatment. After...

journal_title：Biotechnology and bioengineering

authors： Hou XD,Li N,Zong MH

更新日期：2013-07-01 00:00:00

abstract：:In view of a possible application of the alpha-amylase from Bacillus licheniformis as a time-temperature integrator for evaluation of heat processes,(11) thermal inactivation kinetics of the dissolved and covalently immobilized enzyme were studied in the temperature range 90-108 degrees C. The D-values (95 degrees C) ...

journal_title：Biotechnology and bioengineering

authors： De Cordt S,Vanhoof K,Hu J,Maesmans G,Hendrickx M,Tobback P

更新日期：1992-07-01 00:00:00

abstract：:Genetically-engineered cells offer a solution to the cell availability problem in tissue engineering a pancreatic substitute for the treatment of insulin-dependent diabetes. These cells can be non-beta cells, such as hepatocytes or myoblasts, retrieved as a biopsy from the same patient and genetically engineered to se...

journal_title：Biotechnology and bioengineering

authors： Cheng SY,Constantinidis I,Sambanis A

更新日期：2006-04-20 00:00:00

abstract：:The lignin peroxidases (LIP) and manganese peroxidases (MNP) of Phanerochaete chrysosporium catalyze a wide range of lignin depolymerization reactions with lignin models and synthetic lignins in solution. However, their ability to degrade insoluble natural lignin in aqueous media has not been demonstrated. Insoluble i...

journal_title：Biotechnology and bioengineering

authors： Thompson DN,Hames BR,Reddy CA,Grethlein HE

更新日期：1998-03-20 00:00:00

abstract：:The majority of influenza vaccines are manufactured using embryonated hens' eggs. The potential occurrence of a pandemic outbreak of avian influenza might reduce or even eliminate the supply of eggs, leaving the human population at risk. Also, the egg-based production technology is intrinsically cumbersome and not eas...

journal_title：Biotechnology and bioengineering

authors： George M,Farooq M,Dang T,Cortes B,Liu J,Maranga L

更新日期：2010-08-15 00:00:00

abstract：:Analytical expressions describing convective flow in a continuous arteriovenous hollow fiber hemofilter were developed. In the lumen of the hollow fiber membrane, existing analytical expressions were applied to describe velocity profiles and pressure. For flow in the shell (the extracapillary space separating the fibe...

journal_title：Biotechnology and bioengineering

authors： Moussy Y

更新日期：2000-04-20 00:00:00

abstract：:Pseudomonas putida cells were grown in confined volumes in dual-membrane immobilized cell reactors constructed from microporous polyethylene hollow fibers and silicone rubber tubules as a model system for the study of mass transport in microbial aggregates. Local cell concentrations in the reactors reached 300 g dry m...

journal_title：Biotechnology and bioengineering

authors： Karel SF,Robertson CR

更新日期：1989-07-01 00:00:00

abstract：:The design of new generation bioprocessing plants is increasingly dependent on the design of process-compatible microorganisms. The latter, whether through genetic or physiological manipulations, can be greatly assisted by metabolic engineering. An emerging powerful tool in metabolic engineering research is computer-a...

journal_title：Biotechnology and bioengineering

authors： Dean JP,Dervakos GA

更新日期：1998-04-05 00:00:00

abstract：:Polyhydroxyalkanoates (PHA) terployesters P(3HB-co-3HV-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyhexanoate (3HHx) were produced by wild-type Aeromonas hydrophila 4AK4, its recombinant harboring PHA synthesis genes phaPCJ encoding PHA binding protein phasin, PHA synthase, and...

journal_title：Biotechnology and bioengineering

authors： Zhang HF,Ma L,Wang ZH,Chen GQ

更新日期：2009-10-15 00:00:00

abstract：:A flow cytometric kinetic study of hybridoma growth and monoclonal antibody production is presented, along with the influence of glutamine on intracellular responses such as (relative) cell size, and cell RNA and total protein content. Specific findings are: (1) RNA content remained constant throughout the growth phas...

journal_title：Biotechnology and bioengineering

authors： Dalili M,Ollis DF

更新日期：1990-06-05 00:00:00

abstract：:Substrate transfer rates from organic to aqueous phases were measured in the presence and absence of biocatalyst in the reaction medium, using modified Lewis cells. These measurements, in combination with intrinsic aqueous phase biocatalytic reaction kinetics, were used to confirm that benzyl acetate hydrolysis by pig...

journal_title：Biotechnology and bioengineering

authors： Woodley JM,Brazier AJ,Lilly MD

更新日期：1991-01-20 00:00:00

abstract：:Intracellular delivery of functional proteins is of great interest for basic biological research as well as for clinical applications. Transfection is the most commonly used method, however, it is not applicable to large-scale manipulation and inefficient in important cell types implicated in biomedical applications, ...

journal_title：Biotechnology and bioengineering

authors： Li Z,Cai Z,Fu W,Liu Y,Tian C,Wang H,Fu T,Wu Z,Wu D,Jin Y,Cheng Z,Terada N,Liu L,Wu W,Jin S,Bai F

更新日期：2020-03-01 00:00:00

abstract：:Bamboo carbohydrates were hydrolyzed with commercial amylases and a mixture of fungal culture broths containing cellulolytic and hemicellulolytic enzymes. The effects of cooking temperature and the size of fiber particles were also investigated. It was found that the higher the cooking temperature, the higher the rate...

journal_title：Biotechnology and bioengineering

authors： De Menezes TJ,Dos Santos CL,Azzini A

更新日期：1983-04-01 00:00:00