Dynamic pressure method for kla measurement in large-scale bioreactors.

abstract：:Polypeptides containing between 4 and 32 repeats of a resilin-inspired sequence AQTPSSYGAP, derived from the mosquito Anopheles gambiae, have been used as tags on recombinant fusion proteins. These repeating polypeptides were inspired by the repeating structures that are found in resilins and sequence-related proteins...

journal_title：Biotechnology and bioengineering

authors： Lyons RE,Elvin CM,Taylor K,Lekieffre N,Ramshaw JA

更新日期：2012-12-01 00:00:00

abstract：:To gain insight on the fate of arsenic (As) from drinking water treatment residuals in landfills, the mobilization of arsenate adsorbed onto granular ferric hydroxide (GFH) was studied in continuous anaerobic columns fed with a synthetic landfill leachate. The release of As was compared in biologically active and abio...

journal_title：Biotechnology and bioengineering

authors： Cortinas I,Sierra-Alvarez R,Field JA

更新日期：2008-12-15 00:00:00

abstract：:Reducing viscosities of concentrated solutions of therapeutic proteins is important for their subcutaneous and intravenous delivery. Although inorganic salts and optimizing the pH were previously reported to dramatically lower the viscosity of a monoclonal antibody solution, herein we have determined these effects not...

journal_title：Biotechnology and bioengineering

authors： Du W,Klibanov AM

更新日期：2011-03-01 00:00:00

abstract：:Although one of the major factors limiting the application of Bacillus subtilis as an expression host has been its production of at least eight extracellular proteases, researchers have also noticed that some proteases benefited the secretion of foreign proteins at times. Therefore, to maximize the yield of a foreign ...

journal_title：Biotechnology and bioengineering

authors： Zhao L,Ye B,Zhang Q,Cheng D,Zhou C,Cheng S,Yan X

更新日期：2019-08-01 00:00:00

abstract：:MicroRNAs are small non-coding RNAs that play a critical role in post-transcriptional control of gene expression. Recent publications of genomic sequencing data from the Chinese Hamster (CGR) and Chinese hamster ovary (CHO) cells provide new tools for the discovery of novel miRNAs in this important production system. ...

journal_title：Biotechnology and bioengineering

authors： Diendorfer AB,Hackl M,Klanert G,Jadhav V,Reithofer M,Stiefel F,Hesse F,Grillari J,Borth N

更新日期：2015-07-01 00:00:00

abstract：:A key challenge for bioprocess engineering is the identification of the optimum process conditions for the production of biochemical and biopharmaceutical compounds using prokaryotic as well as eukaryotic cell factories. Shake flasks and bench-scale bioreactor systems are still the golden standard in the early stage o...

journal_title：Biotechnology and bioengineering

authors： Totaro D,Rothbauer M,Steiger MG,Mayr T,Wang HY,Lin YS,Sauer M,Altvater M,Ertl P,Mattanovich D

更新日期：2020-07-01 00:00:00

abstract：:The heterogeneous nature of hollow fiber reactors for cell cultivation requires special considerations for proper design and operation. Downstream concentration of high-molecular-weight proteins has been measured in the shell side of ultrafiltration hollow fiber bioreactors. This distribution resulted from shell-side ...

journal_title：Biotechnology and bioengineering

authors： Piret JM,Cooney CL

更新日期：1990-11-01 00:00:00

abstract：:A biotechnological process is described to remove hydrogen sulfide (H(2)S) from high-pressure natural gas and sour gases produced in the petrochemical industry. The process operates at halo-alkaline conditions and combines an aerobic sulfide-oxidizing reactor with an anaerobic sulfate (SO(4) (2-)) and thiosulfate (S(2...

journal_title：Biotechnology and bioengineering

authors： van den Bosch PL,van Beusekom OC,Buisman CJ,Janssen AJ

更新日期：2007-08-01 00:00:00

abstract：:During lignocellulosic ethanol fermentation, yeasts are exposed to various lignocellulose-derived inhibitors, which disrupt the efficiency of hexose and pentose co-fermentation. To understand the metabolic response of fermentation microbes to these inhibitors, a comparative metabolomic investigation was performed on a...

journal_title：Biotechnology and bioengineering

authors： Wang X,Jin M,Balan V,Jones AD,Li X,Li BZ,Dale BE,Yuan YJ

更新日期：2014-01-01 00:00:00

abstract：:The cellulase system of Bacteroides cellulosolvens was subjected to both catabolite repression and feedback inhibition by cellobiose. Cellulose-solubilizing activity was 50% inhibited at a cellobiose concentration of 2.6 g/L and completely inhibited by 12 g/L. Glucose at 12 g/L (the highest concentration tested) had n...

journal_title：Biotechnology and bioengineering

authors： Murray WD

更新日期：1987-06-01 00:00:00

abstract：:Several synthetic metabolic pathways for butanol synthesis have been reported in Escherichia coli by modification of the native CoA-dependent pathway from selected Clostridium species. These pathways are all dependent on the O2 -sensitive AdhE2 enzyme from Clostridium acetobutylicum that catalyzes the sequential reduc...

journal_title：Biotechnology and bioengineering

authors： Pásztor A,Kallio P,Malatinszky D,Akhtar MK,Jones PR

更新日期：2015-01-01 00:00:00

abstract：:Uranium contamination is a serious concern at several sites motivating the development of novel treatment strategies such as the Geobacter-mediated reductive immobilization of uranium. However, this bioremediation strategy has not yet been optimized for the sustained uranium removal. While several reactive-transport m...

journal_title：Biotechnology and bioengineering

authors： Zhao J,Scheibe TD,Mahadevan R

更新日期：2011-07-01 00:00:00

abstract：:As part of an investigation into the use of biological nitrogen fixation for fertilizer ammonia production, continuous culture studies of respiration and nitrogen fixation in the aerobic bacteria Azotobacter vinelandii under oxygen-limited conditions were conducted. Respiration and growth rates followed Monod forms wi...

journal_title：Biotechnology and bioengineering

authors： Diluccio RC,Kirwan DJ

更新日期：1984-01-01 00:00:00

abstract：:It is known that the rolA, rolB, and rolC genes of Agrobacterium rhizogenes T-DNA affect processes of plant development and activate the synthesis of secondary metabolites in transformed plant cells. Although a synergistic activity of the rol genes on root formation is well-documented, little is known about their indi...

journal_title：Biotechnology and bioengineering

authors： Shkryl YN,Veremeichik GN,Bulgakov VP,Tchernoded GK,Mischenko NP,Fedoreyev SA,Zhuravlev YN

更新日期：2008-05-01 00:00:00

abstract：:Generating stable, high-producing cell lines for recombinant protein production requires an understanding of the potential limitations in the cellular machinery for protein expression. In order to increase our understanding of what makes a stable high producer, we have generated a panel of 17 recombinant monoclonal an...

journal_title：Biotechnology and bioengineering

authors： Chusainow J,Yang YS,Yeo JH,Toh PC,Asvadi P,Wong NS,Yap MG

更新日期：2009-03-01 00:00:00

abstract：:A successful procedure for attaching cellulase to 45 microm iron oxide particles with a high-molecular-weight (1000) ligand has been developed. Mass and activity balances were calculated for immobilized enzymes with different loadings. The highest specific activity of the immobilized enzyme was 5.9 mmol glucose/g boun...

journal_title：Biotechnology and bioengineering

authors： Garcia A 3rd,Oh S,Engler CR

更新日期：1989-01-15 00:00:00

abstract：:Molecular descriptors represent structural and physicochemical features of compounds. They have been extensively used for developing statistical models, such as quantitative structure activity relationship (QSAR) and artificial neural networks (NN), for computer prediction of the pharmacodynamic, pharmacokinetic, or t...

journal_title：Biotechnology and bioengineering

authors： Li ZR,Han LY,Xue Y,Yap CW,Li H,Jiang L,Chen YZ

更新日期：2007-06-01 00:00:00

abstract：:The reporter bacterium, Pseudomonas fluorescens HK44 (HK44), was characterized in an immobilized state to investigate utility for deployment as a remote sensor in the subsurface. A packed-bed reactor with alginate-immobilized HK44 simulated hydrodynamic conditions such as might be found in a subsurface environment. Th...

journal_title：Biotechnology and bioengineering

authors： Webb OF,Bienkowski PR,Matrubutham U,Evans FA,Heitzer A,Sayler GS

更新日期：1997-06-05 00:00:00

abstract：:Nitrous oxide (N2 O), a potent greenhouse gas, is reduced to N2 gas by N2 O-reducing bacteria (N2 ORB), a process which represents an N2 O sink in natural and engineered ecosystems. The N2 O sink activity by N2 ORB depends on temperature and O2 exposure, yet the specifics are not yet understood. This study explores th...

journal_title：Biotechnology and bioengineering

authors： Zhou Y,Suenaga T,Qi C,Riya S,Hosomi M,Terada A

更新日期：2020-12-11 00:00:00

abstract：:A mathematical model has been employed to examine the interplay of reaction and mass transfer in immobilized enzyme systems involving reaction-generated enzyme poisions. Deactivation rates can be significantly reduced in some cases by catalyzing a purification reaction in which the poison is transformed into an innocu...

journal_title：Biotechnology and bioengineering

authors： Yeung SY,Cho Y,Bailey JE

更新日期：1978-08-01 00:00:00

abstract：:A spinning cylindrical filter, known as a spinfilter, permits the mammalian cell bioreactor operation at high perfusion rates leading to very high cell densities (10(7) mL(-1)). Filter screens with openings (25 mum) slightly larger than the average cell size have been used to retain single cells in suspension over a l...

journal_title：Biotechnology and bioengineering

authors： Yabannavar VM,Singh V,Connelly NV

更新日期：1994-01-20 00:00:00

abstract：:Capitalizing on components evolved to metabolize ethanol in Aspergillus nidulans, we previously designed the first molecular gas-gene expression interface using gaseous acetaldehyde as the major inducer. This fungus-derived acetaldehyde-inducible gene regulation (AIR) system operated perfectly and enabled precise and ...

journal_title：Biotechnology and bioengineering

authors： Weber W,Spielmann M,Daoud El-Baba M,Keller B,Aubel D,Fussenegger M

更新日期：2005-06-30 00:00:00

abstract：:The use of cell numbers rather than mass to quantify the size of the biotic phase in animal cell cultures causes several problems. First, the cell size varies with growth conditions, thus yields expressed in terms of cell numbers cannot be used in the normal mass balance sense. Second, experience from microbial system...

journal_title：Biotechnology and bioengineering

authors： Nielsen LK,Reid S,Greenfield PF

更新日期：1997-11-20 00:00:00

abstract：:To initiate an infection human immunodeficiency virus type 1 (HIV-1) particles must first bind to receptors on the surface of their host cells, a process that eventually leads to fusion of viral and cellular membranes and release of the viral genome into the cytoplasm. Understanding the molecular mechanisms of these p...

journal_title：Biotechnology and bioengineering

authors： Lim KI,Yin J

更新日期：2006-02-05 00:00:00

abstract：:The kinetic properties of acetate-degrading methanogenic granular sludge of different mean diameters were assessed at different up-flow velocities (V(up)). Using this approach, the influence of internal and external mass transfer could be estimated. First, the apparent Monod constant (K(S)) for each data set was calcu...

journal_title：Biotechnology and bioengineering

authors： Gonzalez-Gil G,Seghezzo L,Lettinga G,Kleerebezem R

更新日期：2001-04-20 00:00:00

abstract：:A model for radial flow mammalian cell culture has been developed. Two situations, one with permeable hollow fibers and the other with a porous matrix in the annulus, were considered. The hollow fibers were modeled as continuously distributed sinks. Numerical solutions are presented for the complete model as well as l...

journal_title：Biotechnology and bioengineering

authors： Tharakan JP,Chau PC

更新日期：1987-04-01 00:00:00

abstract：:The stoichiometry and kinetics of the spontaneous, chemical reaction between pyrite and ferric iron was studied at 30, 45, and 70 degrees C in shake flasks at pH 1.5 by monitoring the ferrous iron, total iron, elemental sulfur, and sulfate concentration profiles in time. It was found that the sulfur moiety of pyrite w...

journal_title：Biotechnology and bioengineering

authors： Boogerd FC,van den Beemd C,Stoelwinder T,Bos P,Kuenen JG

更新日期：1991-06-20 00:00:00

abstract：:More than 80 years after its discovery, penicillin is still a widely used and commercially highly important antibiotic. Here, we analyse the metabolic network of penicillin synthesis in Penicillium chrysogenum based on the concept of elementary flux modes. In particular, we consider the synthesis of the invariant mole...

journal_title：Biotechnology and bioengineering

authors： Prauße MT,Schäuble S,Guthke R,Schuster S

更新日期：2016-01-01 00:00:00

abstract：:Bioaffinity interactions have been, and continue to be, successfully adapted from nature for use in separation and detection applications. It has been previously reported that the magnetophoretic mobility of labeled cells show a saturation type phenomenon as a function of the concentration of the free antibody-magneti...

journal_title：Biotechnology and bioengineering

authors： Zhang H,Williams PS,Zborowski M,Chalmers JJ

更新日期：2006-12-05 00:00:00

abstract：:A strategy to determine effective diffusion coefficients of proteins in chromatographic gels is presented in this article. An experimental methodology based on frontal liquid chromatography was combined with a numerical methodology based on a mathematical model describing the chromatographic process including the extr...

journal_title：Biotechnology and bioengineering

authors： Kempe H,Persson P,Axelsson A,Nilsson B,Zacchi G

更新日期：2006-03-05 00:00:00