Abstract:
:Capitalizing on components evolved to metabolize ethanol in Aspergillus nidulans, we previously designed the first molecular gas-gene expression interface using gaseous acetaldehyde as the major inducer. This fungus-derived acetaldehyde-inducible gene regulation (AIR) system operated perfectly and enabled precise and reversible transgene expression dosing in a variety of mammalian cells. We now validate the use of mainstream cigarette smoke typically containing acetaldehyde at regulation-effective nontoxic concentrations as a noninvasive modality to adjust transgene transcription in mammalian cells and mice. Indeed, tobacco smoke-induced expression fine-tuning of AIR-driven transgenes was successful in mammalian cells. Even mice implanted with cells transgenic for AIR-controlled SEAP (human secreted alkaline phosphatase) production showed serum SEAP levels correlating with inhaled tobacco smoke doses. Tobacco smoke-controlled gene expression may foster clinical opportunities as well as advances in understanding smoke-related pathologies.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Weber W,Spielmann M,Daoud El-Baba M,Keller B,Aubel D,Fussenegger Mdoi
10.1002/bit.20482subject
Has Abstractpub_date
2005-06-30 00:00:00pages
893-7issue
7eissn
0006-3592issn
1097-0290journal_volume
90pub_type
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