Stable recombinant expression of the anti HIV-1 monoclonal antibody 2F5 after IgG3/IgG1 subclass switch in CHO cells.

abstract：:Lipase from Pseudomonas fluorescens biotype I was immobilized by adsorption of anion exchange resin using glutaraldehyde to enhance the adsorption. The activity yield of the immobilized lipase was very low (below 1%) when lipase activity was measured using emulsion substrate. The activity yield was 10-70% when lipase ...

journal_title：Biotechnology and bioengineering

authors： Kosugi Y,Tanaka H,Tomizuka N

更新日期：1990-09-01 00:00:00

abstract：:Exposure of Bacillus subtilis to a shear rate of 1,482/s leads to a rapid loss of cell viability after 10 h of growth. Biochemical and molecular evidences provided below strongly suggest that cell death under high shear results from an apoptosis-like process similar to that described in eukaryotes, with activation of ...

journal_title：Biotechnology and bioengineering

authors： Sahoo S,Rao KK,Suraishkumar GK

更新日期：2006-05-05 00:00:00

abstract：:On-line NAD(P)H fluorescence and culture redox potential (CRP) measurements were utilized to investigate the role of Vitreoscilla hemoglobin (VHb) in perturbing oxygen metabolism of microaerobic Escherichia coli Batch cultures of a VHb-synthesizing E. coli strain and the iso-genic control under fully aerated condition...

journal_title：Biotechnology and bioengineering

authors： Tsai PS,Rao G,Bailey JE

更新日期：1995-08-05 00:00:00

abstract：:A drug-releasing model compound based on photosensitive acrylated ortho-nitrobenzylether (o-NBE) moiety and fluorescein was synthesized to demonstrate photolysis as a mechanism for drug release. Release of this model compound from a hydrogel network can be controlled with light intensity (5-20 mW/cm(2)), exposure dura...

journal_title：Biotechnology and bioengineering

authors： Griffin DR,Patterson JT,Kasko AM

更新日期：2010-12-15 00:00:00

abstract：:Clostridium formicoaceticum homofermentatively converts lactate to acetate at 37 degrees C and pH 6.6-9.6. However, this fermentation is strongly inhibited by acetic acid at acidic pH. The specific growth rate of this organism decreased from a maximum at pH 7.6 to zero at pH 6.6. This inhibition effect was found to be...

journal_title：Biotechnology and bioengineering

authors： Tang IC,Okos MR,Yang ST

更新日期：1989-10-20 00:00:00

abstract：:The first genome scale model (GSM) for Streptomyces leeuwenhoekii C34 was developed to study the biosynthesis pathways of specialized metabolites and to find metabolic engineering targets for enhancing their production. The model, iVR1007, consists of 1,722 reactions, 1,463 metabolites, and 1,007 genes, it includes th...

journal_title：Biotechnology and bioengineering

authors： Razmilic V,Castro JF,Andrews B,Asenjo JA

更新日期：2018-07-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cells are characterized by a low glucose catabolic efficiency, resulting in undesirable lactate production. Here, it is hypothesized that such low efficiency is determined by the transport of pyruvate into the mitochondria. The mitochondrial pyruvate carrier (MPC), responsible for introduci...

journal_title：Biotechnology and bioengineering

authors： Bulté DB,Palomares LA,Parra CG,Martínez JA,Contreras MA,Noriega LG,Ramírez OT

更新日期：2020-09-01 00:00:00

abstract：:In this study, an analysis of the transition between the inactive ("closed") and active ("open") conformations of Candida rugosa lipase in solution is performed using irreversible enzyme inhibitors, cyclic saligenin phosphates. It is shown that >90% inhibition of the enzyme activity toward water-soluble substrates (es...

journal_title：Biotechnology and bioengineering

authors： Turner NA,Needs EC,Khan JA,Vulfson EN

更新日期：2001-01-05 00:00:00

abstract：:We developed a model for nutrient removal in an aerobic granular sludge system. This model can quantitatively describe the start-up of the system by coupling a model for studying the population dynamics of the granules in the reactor (reactor-scale model) and a model for studying the microbial community structure in t...

journal_title：Biotechnology and bioengineering

authors： Kagawa Y,Tahata J,Kishida N,Matsumoto S,Picioreanu C,van Loosdrecht MC,Tsuneda S

更新日期：2015-01-01 00:00:00

abstract：:Saccharomyces cerevisiae (yeast) cells were employed as a source of alcohol dehydrogenase in the NAD(+)-to-NADH reaction. The cells were immobilized in calcium alginate monofilament fibers and used in a biological reactor. The alginate could not be heat sterilized since temperatures above 80 degrees C caused the polym...

journal_title：Biotechnology and bioengineering

authors： Ibbotson PG,Spedding PL,Otterburn MS

更新日期：1990-10-05 00:00:00

abstract：:The effects of dilution rates on the performance of a two-stage fermentation system for a recombinant Escherichia coli culture were studied. Dilution rate determines the apparent or averaged specific growth rate of a heterogeneous population of cells in the recombinant culture. The specific growth rate affects the gen...

journal_title：Biotechnology and bioengineering

authors： Hortacsu A,Ryu DD

更新日期：1991-10-20 00:00:00

abstract：:The C-terminal lysine variation is commonly observed in biopharmaceutical monoclonal antibodies. This modification can be important since it is found to be sensitive to the production process. The methods commonly used to probe this charge variation, including IEF, cIEF, ion-exchange chromatography, and LC-MS, were ev...

journal_title：Biotechnology and bioengineering

authors： Dick LW Jr,Qiu D,Mahon D,Adamo M,Cheng KC

更新日期：2008-08-15 00:00:00

abstract：:In addition to the role of maintaining the pH, buffers can also facilitate the transport of H(+) ions in acid-generating systems. The role of this facilitation in proton transport in porous pellets on acid-generating immobilized enzymic reactions is examined. The activity in these systems can be maximized by a proper ...

journal_title：Biotechnology and bioengineering

authors： Ruckenstein E,Rajora P

更新日期：1985-06-01 00:00:00

abstract：:In large-scale mammalian cell culture, the key toxic by-products assessed and monitored are lactate and ammonia. Often no distinction between the two isoforms of lactate is made. Here, we present profiles of both D- and L-lactate. D-Lactate is the end molecule of the methylglyoxal pathway. D-Lactate unlike L-lactate i...

journal_title：Biotechnology and bioengineering

authors： Paoli T,Faulkner J,O'kennedy R,Keshavarz-Moore E

更新日期：2010-09-01 00:00:00

abstract：:Olive oil hydrolysis using Chromobacterium viscosum lipase B in a reversed micellar media was investigated in a membrane reactor. The dynamic evolution of the product concentration both in the concentrate and permeate stream was analyzed using a mechanistic model previously developed by us and further modified in this...

journal_title：Biotechnology and bioengineering

authors： Prazeres DM,Lemos F,Garcia FA,Cabral JM

更新日期：1993-09-05 00:00:00

abstract：:A spinning cylindrical filter, known as a spinfilter, permits the mammalian cell bioreactor operation at high perfusion rates leading to very high cell densities (10(7) mL(-1)). Filter screens with openings (25 mum) slightly larger than the average cell size have been used to retain single cells in suspension over a l...

journal_title：Biotechnology and bioengineering

authors： Yabannavar VM,Singh V,Connelly NV

更新日期：1994-01-20 00:00:00

abstract：:The biosynthesis of invertase by Saccharomyces carlsbergensis LAM 1068 was studied in relation to its glucose effect at both unsteady and steady states of growth. Experimental correlations between the dilution rate and invertase specific activity (E/X) in chemostat cultures led to an optimum for the enzyme synthesis a...

journal_title：Biotechnology and bioengineering

authors： Toda K

更新日期：1976-08-01 00:00:00

abstract：:Diffusion of phenol through a biofilm attached to activated carbon particles was investigated. The biofilm was grown on activated carbon particles in a draft-tube three-phase fluidized-bed bioreactor operating in a fed-batch mode. It was found that phenol did not adsorb on the biofilm and that the diffusion coefficien...

journal_title：Biotechnology and bioengineering

authors： Fan LS,Leyva-Ramos R,Wisecarver KD,Zehner BJ

更新日期：1990-02-05 00:00:00

abstract：:This study examined the hydrodynamic characteristics of a liquid-solid fluidized-bed bioreactor using elastic particles (PVA gel beads) of various diameters as carriers. The drag coefficient-Reynolds number, velocity-voidage, and expansion index-Reynolds number relationships observed during fluidization of PVA gel bea...

journal_title：Biotechnology and bioengineering

authors： Wu JY,Chen KC,Chen CT,Hwang SC

更新日期：2003-09-05 00:00:00

abstract：:A uniquely simple model is developed to describe the NO(3) (-) and NO(2) (-) concentration profiles within a dentrification fluidized bed biological reactor. This simple model is compared to experimental data, and to a more complex model similar to those previously proposed in the literature. The simple model fits the...

journal_title：Biotechnology and bioengineering

authors： Brosilow BJ,Schnitzer M,Tarre S,Green M

更新日期：1997-06-20 00:00:00

abstract：:In order to evaluate the sensitivity of hepatocellular cultures to variations in both substrate stiffness and bioactive ligand presentation, hepatocytes were cultured on differentially compliant polyacrylamide gel discs functionalized with varying amounts of the ECM ligand, fibronectin (FN). Subconfluent cell cultures...

journal_title：Biotechnology and bioengineering

authors： Semler EJ,Lancin PA,Dasgupta A,Moghe PV

更新日期：2005-02-05 00:00:00

abstract：:The utilization and conversion of D-xylose, D-xylulose, L-arabinose, and xylitol by yeast strains have been investigated with the following results: (1) The majority of yeasts tested utilize D-xylose and produce polyols, ethanol, and organic acids. The type and amount of products formed varies with the yeast strains u...

journal_title：Biotechnology and bioengineering

authors： Gong CS,Claypool TA,McCracken LD,Maun CM,Ueng PP,Tsao GT

更新日期：1983-01-01 00:00:00

abstract：:Recombinant retroviruses are effective vectors able to integrate transgenes into the target cell's genome to achieve longer-term expression. This study investigates the effect of cell lysis products, a common cell culture by-product, on the transduction of suspension cells by gammaretroviral vectors. Cell lysates deri...

journal_title：Biotechnology and bioengineering

authors： Beauchesne PR,Bruce KJ,Bowen BD,Piret JM

更新日期：2010-04-15 00:00:00

abstract：:The amount of ATP produced during n-alkane, fatty acid, or acetate metabolism in Candida tropicalis has been established from the P/O ratios measured on isolated mitochondria, yield on substrate and carbon balance. For these three kinds of substrates YATP value has been found to be close to 4, although Ysub on acetate...

journal_title：Biotechnology and bioengineering

authors： Gallo M,Azoulay E

更新日期：1975-12-01 00:00:00

abstract：:Streamline Direct CST I is a new type of ion exchanger with multi-modal functional groups, specially designed for an expanded bed adsorption (EBA) process, which can capture directly the proteins from the high ionic strength feedstocks with a high binding capacity. In this study, an experimental study is carried out f...

journal_title：Biotechnology and bioengineering

authors： Li P,Xiu G,Mata VG,Grande CA,Rodrigues AE

更新日期：2006-08-20 00:00:00

abstract：:Traditional biological treatment models are "deduced" from formal chemical kinetics or dynamics of pure microorganism cultures growth. The best formal models give reasonable approximations of the biological treatment model with an ecosystem adaptation (ESA model). The model presented here explains some features of the...

journal_title：Biotechnology and bioengineering

authors： Vavilin VA

更新日期：1983-06-01 00:00:00

abstract：:Trehalase is the enzyme which hydrolyzes the disaccharide trehalose into two alpha-D-glucose molecules. In this article, we present the immobilization of trehalase on aminopropyl glass particles. The enzyme was extracted from Escherichia coli Mph2, a strain harboring the pTRE11 plasmid, which contains the trehalase ge...

journal_title：Biotechnology and bioengineering

authors： Bachinski N,Martins AS,Paschoalin VM,Panek AD,Paiva CL

更新日期：1997-04-05 00:00:00

abstract：:Pretreatment of sugar cane bagasse is essential for a simultaneous saccharification and fermentation (SSF) process which uses recombinant Klebsiella oxytoca strain P2 and Genencor Spezyme CE. Strain P2 has been genetically engineered to express Zymomonas mobilis genes encoding the ethanol pathway and retains the nativ...

journal_title：Biotechnology and bioengineering

authors： Doran JB,Aldrich HC,Ingram LO

更新日期：1994-06-20 00:00:00

abstract：:Dilute aqueous solutions of polymers released by marine phytoplankton (microalgae) were shown to effectively reduce drag in capillary pipe flow. Tests were performed in a capillary turbulent flow viscometer which extruded small samples under high pressures. In all, 22 species were screened, and the products of one chl...

journal_title：Biotechnology and bioengineering

authors： Ramus J,Kenney BE,Shaughnessy EJ

更新日期：1989-01-25 00:00:00

abstract：:The effect of the water content on the lipase-catalyzed (Candida rugosa) interesterification (acidolysis) of menhaden oil with conjugated linoleic acid was studied for amounts of added water ranging from 0-4% (w/w). The rate of the acidolysis reaction increased with increasing water content, but the corresponding perc...

journal_title：Biotechnology and bioengineering

authors： Torres CF,Hill CG Jr

更新日期：2002-06-05 00:00:00