Drag reducing properties of microalgal exopolymers.

abstract：:A mathematical model of glycolysis in Saccharomyces cerevisiae is presented. The model is based on rate equations for the individual reactions and aims to predict changes in the levels of intra- and extracellular metabolites after a glucose pulse, as described in part I of this study. Kinetic analysis focuses on a tim...

journal_title：Biotechnology and bioengineering

authors： Rizzi M,Baltes M,Theobald U,Reuss M

更新日期：1997-08-20 00:00:00

abstract：:Production of conidia of the biocontrol fungus Coniothyrium minitans by solid-state cultivation in a packed-bed reactor on an industrial scale is feasible. Spore yield and oxygen consumption rate of C. minitans during cultivation on oats and three inert solids (hemp, perlite, and bagasse) saturated with a liquid mediu...

journal_title：Biotechnology and bioengineering

authors： Weber FJ,Tramper J,Rinzema A

更新日期：1999-11-20 00:00:00

abstract：:Hydrogel membranes have been fabricated that incorporate antibody/antigen moieties. The permeability of large solutes through these membranes is dependent on the presence of soluble antigen that can compete with the internal interactions between antibody and antigen leading to an increase in gel mesh size. Specificall...

journal_title：Biotechnology and bioengineering

authors： Zhang R,Bowyer A,Eisenthal R,Hubble J

更新日期：2007-07-01 00:00:00

abstract：:Screening fifteen strains of white-rot fungi for their ability to decolorize combined bleached kraft effluent showed that Coriolus versicolor in liquid culture removed over 60% of the color of the effluent within six days in the presence of sucrose. Treatment of the same effluent with this fungus, immobilized in beads...

journal_title：Biotechnology and bioengineering

authors： Livernoche D,Jurasek L,Desrochers M,Dorica J,Veliky IA

更新日期：1983-08-01 00:00:00

abstract：:We designed a bioartificial liver support system in which encapsulated multicellular spheroids of rat hepatocytes were utilized as a bioreactor in a hollow fiber cartridge. The spheroids, formed in a positively charged polystyrene dish that contained hormonally defined medium, were encapsulated into microdroplets of a...

journal_title：Biotechnology and bioengineering

authors： Shiraha H,Koide N,Hada H,Ujike K,Nakamura M,Shinji T,Gotoh S,Tsuji T

更新日期：1996-05-20 00:00:00

abstract：:In a stirred culture of hybridoma cells, the effects of serum reduction from 2.5% to 0% on growth and monoclonal antibody production have been investigated. The shear sensitivity of cells from the same culture has been tested in a bubble column. Serum reduction does not greatly affect viable-cell concentrations, but c...

journal_title：Biotechnology and bioengineering

authors： van der Pol L,Bakker WA,Tramper J

更新日期：1992-06-05 00:00:00

abstract：:Mucopolysaccharidosis (MPS) Type II is caused by mutations in the gene encoding the lysosomal enzyme, iduronate 2-sulfatase (IDS). The majority of MPSII cases affect the brain. However, enzyme replacement therapy with recombinant IDS does not treat the brain, because IDS is a large molecule drug that does not cross th...

journal_title：Biotechnology and bioengineering

authors： Boado RJ,Ka-Wai Hui E,Zhiqiang Lu J,Pardridge WM

更新日期：2014-11-01 00:00:00

abstract：:Rare sugars have various known biological functions and potential for applications in pharmaceutical, cosmetics, and food industries. Here we designed and constructed a recombination pathway in Corynebacterium glutamicum, in which dihydroxyacetone phosphate (DHAP), an intermediate of the glycolytic pathway, and a vari...

journal_title：Biotechnology and bioengineering

authors： Yang J,Zhu Y,Li J,Men Y,Sun Y,Ma Y

更新日期：2015-01-01 00:00:00

abstract：:Pretreatment of rice straw by using renewable cholinium amino acids ionic liquids ([Ch][AA] ILs)-water mixtures and the subsequent enzymatic hydrolysis of the residues were conducted in the present work. Of the eight mixtures composed of ILs and water, most were found to be effective for rice straw pretreatment. After...

journal_title：Biotechnology and bioengineering

authors： Hou XD,Li N,Zong MH

更新日期：2013-07-01 00:00:00

abstract：:In the last few years, an increasing number of biotechnological techniques have been applied to the restoration and conservation of works of art, paintings, old maps, and papers or books. Enzymes can solve problems that give restorers difficulties, although for many applications it is not possible to use soluble enzym...

journal_title：Biotechnology and bioengineering

authors： Moeschel K,Nouaimi M,Steinbrenner C,Bisswanger H

更新日期：2003-04-20 00:00:00

abstract：:Tumor-targeted Salmonella VNP20009 preferentially replicate within tumor tissue and partially suppress tumor growth in murine tumor models. These Salmonella have the ability to locally induce apoptosis when they are in direct contact with cancer cells but they lack significant bystander killing, which may correlate wi...

journal_title：Biotechnology and bioengineering

authors： Quintero D,Carrafa J,Vincent L,Bermudes D

更新日期：2016-12-01 00:00:00

abstract：:The behavior of 20 beta-hydroxysteroid dehydrogenase in a two-phase system consisting of an emulsion of water with an immiscible organic solvent was investigated. The effect of several organic solvents on the stability, activity, and kinetic profile of the enzyme was considered. The most suitable system for carrying o...

journal_title：Biotechnology and bioengineering

authors： Cremonesi P,Carrea G,Ferrara L,Antonini E

更新日期：1975-08-01 00:00:00

abstract：:Intracellular delivery of functional proteins is of great interest for basic biological research as well as for clinical applications. Transfection is the most commonly used method, however, it is not applicable to large-scale manipulation and inefficient in important cell types implicated in biomedical applications, ...

journal_title：Biotechnology and bioengineering

authors： Li Z,Cai Z,Fu W,Liu Y,Tian C,Wang H,Fu T,Wu Z,Wu D,Jin Y,Cheng Z,Terada N,Liu L,Wu W,Jin S,Bai F

更新日期：2020-03-01 00:00:00

abstract：:A simple approach was developed to determine the half-saturation coefficient for dissolved oxygen (K(DO)) for three bacteria by maintaining a constant oxygen concentration in continuous culture, and employing a dynamic method to obtain the specific growth rate (mu) for each species. Measurement of mu at selected disso...

journal_title：Biotechnology and bioengineering

authors： Hao OJ,Richard MG,Jenkins D,Blanch HW

更新日期：1983-02-01 00:00:00

abstract：:Two kinds of disposable bioreactors, air-lift disposable bioreactors (ADB) and wave disposable bioreactors (WDB) were compared with stirred-tank reactors (5-L STR). These bioreactors were successfully applied to transgenic rice cell cultures for the production of recombinant human cytotoxic T-lymphocyte antigen 4-immu...

journal_title：Biotechnology and bioengineering

authors： Kwon JY,Yang YS,Cheon SH,Nam HJ,Jin GH,Kim DI

更新日期：2013-09-01 00:00:00

abstract：:Two methods employing a water-soluble carbodiimide for carboxyl activation were investigated for the immobilization of biochemicals to succinamidopropyl-porous glass beads. Immobilization using the simultaneous method (simultaneous addition of carbodiimide and nucleophilic ligand to the beads) and large excess of carb...

journal_title：Biotechnology and bioengineering

authors： Janolino VG,Swaisgood HE

更新日期：1982-05-01 00:00:00

abstract：:The effect of ligand density on anion-exchange membrane chromatography (AEXmc) for the purification of recombinant baculoviruses (rBVs), potential viral vectors in clinical applications, is studied by surface plasmon resonance on customized AEX surfaces and gradient elution experiments on Sartobind D membrane prototyp...

journal_title：Biotechnology and bioengineering

authors： Vicente T,Fáber R,Alves PM,Carrondo MJ,Mota JP

更新日期：2011-06-01 00:00:00

abstract：:The use of cell numbers rather than mass to quantify the size of the biotic phase in animal cell cultures causes several problems. First, the cell size varies with growth conditions, thus yields expressed in terms of cell numbers cannot be used in the normal mass balance sense. Second, experience from microbial system...

journal_title：Biotechnology and bioengineering

authors： Nielsen LK,Reid S,Greenfield PF

更新日期：1997-11-20 00:00:00

abstract：:Nitrous oxide (N2 O), a potent greenhouse gas, is reduced to N2 gas by N2 O-reducing bacteria (N2 ORB), a process which represents an N2 O sink in natural and engineered ecosystems. The N2 O sink activity by N2 ORB depends on temperature and O2 exposure, yet the specifics are not yet understood. This study explores th...

journal_title：Biotechnology and bioengineering

authors： Zhou Y,Suenaga T,Qi C,Riya S,Hosomi M,Terada A

更新日期：2020-12-11 00:00:00

abstract：:By feeding ethanol at various high rates to low cell density cultures of Saccharomyces cerevisiae it was shown that the sharp fall in viability when ethanol is produced during rapid fermentations is in part a direct consequence of the high rate of change of extracellular ethanol concentration. Nevertheless, the fall i...

journal_title：Biotechnology and bioengineering

authors： Dasari G,Worth MA,Connor MA,Pamment NB

更新日期：1990-01-20 00:00:00

abstract：:Compared to the conventional microbial and mammalian systems, transgenic plants produce proteins in a different matrix. This provides opportunities and challenges for downstream processing. In the context of the plant host Brassica napus (canola), this work addresses the bioprocessing challenges of solid fractionation...

journal_title：Biotechnology and bioengineering

authors： Bai Y,Glatz CE

更新日期：2003-03-30 00:00:00

abstract：:Biomass autoflocculation in outdoor algal cultures was found to be associated with increases of culture pH levels, due to CO(2) consumption by the algal photosynthetic activity. Under these alkaline conditions, some medium chemical ions precipitated together with the algal biomass. The chemical substances involved wit...

journal_title：Biotechnology and bioengineering

authors： Sukenik A,Shelef G

更新日期：1984-02-01 00:00:00

abstract：:Extraction of intracellular protein from Escherichia coli is traditionally achieved by mechanical disruption. A chemical treatment that destroys the integrity of the bacterial cell wall and could provide an alternative technique is examined in this study. Treatment with a combination of the chelating agent ethylenedia...

journal_title：Biotechnology and bioengineering

authors： Falconer RJ,O'Neill BK,Middelberg AP

更新日期：1997-03-05 00:00:00

abstract：:The simultaneous growth and product formation in a microbial culture is an important feature of several laboratory, industrial, and environmental bioprocesses. Metabolic burden associated with product formation in these bioprocesses may lead to growth advantage of a nonproducing mutant leading to a loss of the produci...

journal_title：Biotechnology and bioengineering

authors： Varma A,Palsson BO

更新日期：1994-02-20 00:00:00

abstract：:Hemicellulose residues can be hydrolyzed into a sugar syrup using dilute mineral acids. Although this syrup represents a potential feedstock for biofuel production, toxic compounds generated during hydrolysis limit microbial metabolism. Escherichia coli LY01, an ethanologenic biocatalyst engineered to ferment the mixe...

journal_title：Biotechnology and bioengineering

authors： Zaldivar J,Ingram LO

更新日期：1999-01-01 00:00:00

abstract：:Although widely used in experimental and industrial situations, genetically engineered plasmids containing the lac promoter from Escherichia coli are subject to catabolite repression when grown in glucose-containing media. Several methods of overcoming this problem have been investigated by studying the expression of ...

journal_title：Biotechnology and bioengineering

authors： Warnes A,Stephenson JR,Fooks AR,Melling J,Brown MR

更新日期：1991-11-01 00:00:00

abstract：:Engineering protein expression in vitro or in vivo is usually straightforward for single genes, but remains challenging for multiple genes because of the requirement of coordinated control. RNA and protein overexpression strategies often exploit T7 RNA polymerase and its natural TPhi Class I terminator. However, this ...

journal_title：Biotechnology and bioengineering

authors： Du L,Gao R,Forster AC

更新日期：2009-12-15 00:00:00

abstract：:We show the design features of a membrane bioreactor based on pulsatile flow across dimpled membranes. Results show an enhanced mass transfer of air of at least five-fold magnitude as compared with flat membranes. An increased working volume form 20 mL to 120 mL reduced the k(L)A at a given Reynolds number because of ...

journal_title：Biotechnology and bioengineering

authors： Beeton S,Millward HR,Bellhouse BJ,Nicholson AM,Jenkins N,Knowles CJ

更新日期：1991-12-05 00:00:00

abstract：:Bamboo carbohydrates were hydrolyzed with commercial amylases and a mixture of fungal culture broths containing cellulolytic and hemicellulolytic enzymes. The effects of cooking temperature and the size of fiber particles were also investigated. It was found that the higher the cooking temperature, the higher the rate...

journal_title：Biotechnology and bioengineering

authors： De Menezes TJ,Dos Santos CL,Azzini A

更新日期：1983-04-01 00:00:00

abstract：:For the application of immobilized enzymes, fixed bed reactors are used almost exclusively. Fixed bed reactors have specific disadvantages, especially for processes with a deactivating catalyst. Therefore, we have studied a novel reactor type with continuous transport of the immobilized biocatalyst. Flow of biocatalys...

journal_title：Biotechnology and bioengineering

authors： Vos HJ,Groen DJ,Potters JJ,Luyben KC

更新日期：1990-08-05 00:00:00