Cell cycle model to describe animal cell size variation and lag between cell number and biomass dynamics.

abstract：:The simultaneous growth and product formation in a microbial culture is an important feature of several laboratory, industrial, and environmental bioprocesses. Metabolic burden associated with product formation in these bioprocesses may lead to growth advantage of a nonproducing mutant leading to a loss of the produci...

journal_title：Biotechnology and bioengineering

authors： Varma A,Palsson BO

更新日期：1994-02-20 00:00:00

abstract：:Production of conidia of the biocontrol fungus Coniothyrium minitans by solid-state cultivation in a packed-bed reactor on an industrial scale is feasible. Spore yield and oxygen consumption rate of C. minitans during cultivation on oats and three inert solids (hemp, perlite, and bagasse) saturated with a liquid mediu...

journal_title：Biotechnology and bioengineering

authors： Weber FJ,Tramper J,Rinzema A

更新日期：1999-11-20 00:00:00

abstract：:Kluyveromyces marxianus UCD (FST) 55-82 cells were immobilized in Na alginate beads and used in a packed-bed bioreactor system for the continuous production of ethanol from the extract of Jerusalem artichoke tubers. Volumetric ethanol productivities of 104 and 80 g ethanol/ L/h were obtained at 80 and 92% sugar utiliz...

journal_title：Biotechnology and bioengineering

authors： Margaritis A,Bajpai P

更新日期：1982-07-01 00:00:00

abstract：:The reconstruction of functioning human tissues ex vivo is becoming an important part of biotechnology. There are compelling scientific, clinical, and biotechnological reasons for fully or partially reconstituting human tissues such as skin, bone marrow, and liver ex vivo. In particular, bone marrow is a tissue of muc...

journal_title：Biotechnology and bioengineering

authors： Koller MR,Palsson BO

更新日期：1993-10-01 00:00:00

abstract：:In recent years there has been a considerable interest in developing membrane chromatography systems that function as a short, wide chromatographic column in which the adsorptive packing consists of one or more microporous membranes. This study reports the use of new adsorber membranes prepared by the incorporation of...

journal_title：Biotechnology and bioengineering

authors： Avramescu ME,Borneman Z,Wessling M

更新日期：2003-12-05 00:00:00

abstract：:A flow cytometric kinetic study of hybridoma growth and monoclonal antibody production is presented, along with the influence of glutamine on intracellular responses such as (relative) cell size, and cell RNA and total protein content. Specific findings are: (1) RNA content remained constant throughout the growth phas...

journal_title：Biotechnology and bioengineering

authors： Dalili M,Ollis DF

更新日期：1990-06-05 00:00:00

abstract：:The amount of ATP produced during n-alkane, fatty acid, or acetate metabolism in Candida tropicalis has been established from the P/O ratios measured on isolated mitochondria, yield on substrate and carbon balance. For these three kinds of substrates YATP value has been found to be close to 4, although Ysub on acetate...

journal_title：Biotechnology and bioengineering

authors： Gallo M,Azoulay E

更新日期：1975-12-01 00:00:00

abstract：:Cartilage implants for potential in vivo use for joint repair or reconstructive surgery can be created in vitro by growing chondrocytes on biodegradable polymer scaffolds. Implants 1 cm in diameter by 0.176 cm thick were made using isolated calf chondrocytes and polyglucolic acid (PGA). By 6 weeks, the total amount of...

journal_title：Biotechnology and bioengineering

authors： Freed LE,Marquis JC,Langer R,Vunjak-Novakovic G,Emmanual J

更新日期：1994-03-25 00:00:00

abstract：:Metabolic engineering of isopropyl alcohol (IPA)-producing Escherichia coli strains was conducted along with 13 C-metabolic flux analysis (MFA). A metabolically engineered E. coli strain expressing the adc gene derived from Clostridium acetobutylicum and the IPADH gene from C. beijerinckii did not produce IPA during i...

journal_title：Biotechnology and bioengineering

authors： Okahashi N,Matsuda F,Yoshikawa K,Shirai T,Matsumoto Y,Wada M,Shimizu H

更新日期：2017-12-01 00:00:00

abstract：:In eukaryotes, secretory proteins are folded and assembled in the endoplasmic reticulum (ER). Many heterologous proteins are retained in the ER due to suboptimal folding conditions. We previously reported that heterologous secretion of Pyrococcus furiosus beta-glucosidase in Saccharomyces cerevisiae resulted in the ac...

journal_title：Biotechnology and bioengineering

authors： Smith JD,Tang BC,Robinson AS

更新日期：2004-02-05 00:00:00

abstract：:Two strains of Pseudomonas able to grow on phenol or p-nitrophenol (PNP) were isolated from sewage. Pseudomonas sp. PN101 mineralized and formed nitrite from PNP but did not mineralize phenol, and Pseudomonas sp. PH111 mineralized phenol but not PNP. Phenol increased the lag period before Pseudomonas sp. PN101 grew on...

journal_title：Biotechnology and bioengineering

authors： Murakami Y,Alexander M

更新日期：1989-02-20 00:00:00

abstract：:In this study, a useful method was developed to fabricate array patterns of microparticles not on electrode surfaces, but on arbitrary surfaces, using negative-dielectrophoresis (n-DEP). First, electrodes were designed and electric field simulations were performed to manipulate microparticles toward target areas. Base...

journal_title：Biotechnology and bioengineering

authors： Ino K,Shiku H,Ozawa F,Yasukawa T,Matsue T

更新日期：2009-11-01 00:00:00

abstract：:Macroencapsulation technology has been an attractive topic in the field of treatment for Type 1 diabetes due to mechanical stability, versatility, and retrievability of the macro-capsule design. Macro-capsules can be categorized into extravascular and intravascular devices, in which solute transport relies either on d...

journal_title：Biotechnology and bioengineering

authors： Song S,Roy S

更新日期：2016-07-01 00:00:00

abstract：:To monitor gas reaction rates in animal cell culture at constant dissolved oxygen concentration (DO) and constant pH it was necessary to develop improved control methods. Decoupling of both controllrs was obtained by manipulation of molar fractions of oxygen and carbon dioxide in the gas phase. Two pairs of DO and pH ...

journal_title：Biotechnology and bioengineering

authors： Oeggerli A,Eyer K,Heinzle E

更新日期：1995-01-05 00:00:00

abstract：:Regenerating damaged tissue interfaces remains a significant clinical challenge, requiring recapitulation of the structure, composition, and function of the native enthesis. In the ligament-to-bone interface, this region transitions from ligament to fibrocartilage, to calcified cartilage and then to bone. This gradati...

journal_title：Biotechnology and bioengineering

authors： Harris E,Liu Y,Cunniffe G,Morrissey D,Carroll S,Mulhall K,Kelly DJ

更新日期：2017-10-01 00:00:00

abstract：:A new method is described for the immobilization of biologically active proteins onto several types of organic polymer beads. First, the soluble protein is modified by reaction with an excess of a hydrophobic imidoester, for example methyl 4-phenyl-butyrimidate, at ca. pH 9 and 0 degrees . Excess imidoester and side p...

journal_title：Biotechnology and bioengineering

authors： Ampon K,Means GE

更新日期：1988-08-20 00:00:00

abstract：:Bioluminescence from the lux-based bacterial reporter Pseudomonas fluorescens HK44 was experimentally investigated under growth substrate-rich and limiting conditions in batch, continuous stirred tank (CSTR), and turbidostat reactors. A mechanistically based, mathematical model was developed to describe bioluminescenc...

journal_title：Biotechnology and bioengineering

authors： Kelly CJ,Hsiung CJ,Lajoie CA

更新日期：2003-02-05 00:00:00

abstract：:This study conducted quantitative kinetic modeling and in situ and temporally resolved measurements of adsorption, desorption, and re-adsorption of a commercial endoglucanase in lignocellulosic suspensions. The study defined a cellulase adsorption and desorption competition parameter, a pseudo rate of binding and deso...

journal_title：Biotechnology and bioengineering

authors： Wang QQ,Zhu JY,Hunt CG,Zhan HY

更新日期：2012-08-01 00:00:00

abstract：:Microplates with integrated optical oxygen sensors are a new tool to study metabolic rates and enzyme activities. Precise measurements are possible only if oxygen exchange between the sample and the environment is known. In this study we quantify gas exchange in plastic microplates. Dissolved oxygen was detected using...

journal_title：Biotechnology and bioengineering

authors： Arain S,Weiss S,Heinzle E,John GT,Krause C,Klimant I

更新日期：2005-05-05 00:00:00

abstract：:A chimeric Fab was expressed in Chinese hamster ovary cells under the control of the CMV promoter in a two-stage production process. Cells were first grown to 90% confluence at 37 degrees C in a proliferation phase, followed by a production phase at either 37 degrees C or 28 degrees C. Medium supplemented with serum a...

journal_title：Biotechnology and bioengineering

authors： Schatz SM,Kerschbaumer RJ,Gerstenbauer G,Kral M,Dorner F,Scheiflinger F

更新日期：2003-11-20 00:00:00

abstract：:Squalene is a lipophilic and non-volatile triterpene with many industrial applications for food, pharmaceuticals, and cosmetics. Metabolic engineering focused on optimization of the production pathway suffer from little success in improving titers because of a limited space of the cell membrane accommodating the lipop...

journal_title：Biotechnology and bioengineering

authors： Meng Y,Shao X,Wang Y,Li Y,Zheng X,Wei G,Kim SW,Wang C

更新日期：2020-07-21 00:00:00

abstract：:In vivo mesenchymal stem cell (MSC) survival is relevant to therapeutic applications requiring engraftment and potentially to nonengraftment applications as well. MSCs are a mixture of progenitors at different stages of cellular aging, but the contribution of this heterogeneity to the survival of MSC implants is unkno...

journal_title：Biotechnology and bioengineering

authors： Madsen SD,Jones SH,Tucker HA,Giler MK,Muller DC,Discher CT,Russell KC,Dobek GL,Sammarco MC,Bunnell BA,O'Connor KC

更新日期：2020-01-01 00:00:00

abstract：:The aim of this study was to determine the feasibility of using high pressure CO2 for sterilization of Ginseng powder, as an alternative method to conventional techniques such as gamma-irradiation and ethylene oxide. The Ginseng sample used in this study was originally contaminated with fungi and 5 x 10(7) bacteria/g ...

journal_title：Biotechnology and bioengineering

authors： Dehghani F,Annabi N,Titus M,Valtchev P,Tumilar A

更新日期：2009-02-01 00:00:00

abstract：:The influence of solution Eh on the rate of ferrous iron oxidation by Acidithiobacillus ferrooxidans is characterized. The experimental approach was based on the use of a two-chamber bioelectrochemical cell, which can determine the ferrous iron oxidation rate at controlled potential. Results enabled the formulation of...

journal_title：Biotechnology and bioengineering

authors： Meruane G,Salhe C,Wiertz J,Vargas T

更新日期：2002-11-05 00:00:00

abstract：:Urea, ammonium, and free amino acid contents were quantified in biological aging of a young wine under two flor film forming yeast strains, Saccharomyces cerevisiae race capensis and S. cerevisiae race bayanus, and compared. Cell viability in the film was different for the two yeast strains. Thus, capensis maintained ...

journal_title：Biotechnology and bioengineering

authors： Mauricio JC,Ortega JM

更新日期：1997-01-20 00:00:00

abstract：:The catalytic properties of bovine serum albumin (BSA) have been studied rather extensively. The protein has shown catalytic activities toward a variety of reactions in aqueous media. However, there appears to be no reports of reactions catalyzed exclusively in organic media. In this study, we report the catalytic pro...

journal_title：Biotechnology and bioengineering

authors： Liu XC,Lemma-Gray P

更新日期：2001-09-20 00:00:00

abstract：:This study demonstrates that masking the Feb regions of a monoclonal antibody (Mab) with synthetic antigens prior to covalent immobilization efficiency. Water-soluble adducts of poly(2-methyloxazoline) polymers and a synthetic-peptide epitope for the Mab were constructed. These synthetic antigens are referred to as Fa...

journal_title：Biotechnology and bioengineering

authors： Velander WH,Subramanian A,Madurawe RD,Orthner CL

更新日期：1992-04-25 00:00:00

abstract：:Genetically engineered microbes have been intensively investigated as a means for the cost-effective production of isoprenoids. Bacillus subtilis is a promising microbial host for this purpose because of its fast growth rate and GRAS (generally regarded as safe) status. To date, development of this host has been impai...

journal_title：Biotechnology and bioengineering

authors： Zhou K,Zou R,Zhang C,Stephanopoulos G,Too HP

更新日期：2013-09-01 00:00:00

abstract：:Highly branched mutants of two strains of Aspergillus oryzae (IFO4177, which produces alpha-amylase, and a transformant of IFO4177 [AMG#13], which produces heterologous glucoamylase in addition to alpha-amylase) were generated by UV or nitrous acid mutagenesis. Four mutants of the parental strain (IFO4177), which were...

journal_title：Biotechnology and bioengineering

authors： Bocking SP,Wiebe MG,Robson GD,Hansen K,Christiansen LH,Trinci AP

更新日期：1999-12-20 00:00:00

abstract：:The Model presented in this work demonstrates the combination of cell-cycle model with a model describing the growth and conversion kinetics of hybridoma cells in a steady-state continuous culture. The cell-cycle model is based upon a population balance model as described by Cazzador et al. and assumes the existence o...

journal_title：Biotechnology and bioengineering

authors： Martens DE,Sipkema EM,de Gooijer CD,Beuvery EC,Tramper J

更新日期：1995-10-05 00:00:00