Abstract:
:A chimeric Fab was expressed in Chinese hamster ovary cells under the control of the CMV promoter in a two-stage production process. Cells were first grown to 90% confluence at 37 degrees C in a proliferation phase, followed by a production phase at either 37 degrees C or 28 degrees C. Medium supplemented with serum and medium free from serum was tested in the production phase at both temperatures. Comparison of Fab expression revealed that reducing the temperature to 28 degrees C resulted in a 14-fold increase in product yield when cells were cultivated in serum-containing medium, and in a 38-fold increase in product yield when serum-free medium was applied.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Schatz SM,Kerschbaumer RJ,Gerstenbauer G,Kral M,Dorner F,Scheiflinger Fdoi
10.1002/bit.10793subject
Has Abstractpub_date
2003-11-20 00:00:00pages
433-8issue
4eissn
0006-3592issn
1097-0290journal_volume
84pub_type
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