Technical evaluation of the potential for streamlining of equipment validation for fermentation applications.

abstract：:The influence of microheterogeneity on enzyme inactivation kinetics is examined. A continuous normal distribution of the thermal activation energy is assumed, and using this, a simple mathematical model is developed to find the activity-time trajectories for a microheterogeneous enzyme. Using an example, the model is ...

journal_title：Biotechnology and bioengineering

authors： Malhotra A,Sadana A

更新日期：1987-07-01 00:00:00

abstract：:A refolding strategy was described for on-column refolding of recombinant human interferon-gamma (rhIFN-gamma) inclusion bodies by expanded bed adsorption (EBA) chromatography. After the denatured rhIFN-gamma protein bound onto the cation exchanger of STREAMLINE SP, the refolding process was performed in expanded bed ...

journal_title：Biotechnology and bioengineering

authors： Jin T,Guan YX,Yao SJ,Lin DQ,Cho MG

更新日期：2006-03-05 00:00:00

abstract：:Disulfide-rich peptides isolated from cone snails are of great interest as drug leads due to their high specificity and potency toward therapeutically relevant ion channels and receptors. They commonly contain the inhibitor cystine knot (ICK) motif comprising three disulfide bonds forming a knotted core. Here we repor...

journal_title：Biotechnology and bioengineering

authors： Kwon S,Bosmans F,Kaas Q,Cheneval O,Conibear AC,Rosengren KJ,Wang CK,Schroeder CI,Craik DJ

更新日期：2016-10-01 00:00:00

abstract：:Non-viral delivery of cell-impermeant drugs and DNA in vivo has traditionally relied upon either chemical or physical stress applied directly to target tissues. Physical methods typically use contact between an applicator, or electrode, and the target tissue and may involve patient discomfort. To overcome contact-depe...

journal_title：Biotechnology and bioengineering

authors： Connolly RJ,Lopez GA,Hoff AM,Jaroszeski MJ

更新日期：2009-12-01 00:00:00

abstract：:Three-dimensional cell culture models, such as spheroids, can be used in the process of the development of new anticancer agents because they are able to closely mimic the main features of human solid tumors, namely their structural organization, cellular layered assembling, hypoxia, and nutrient gradients. These prop...

journal_title：Biotechnology and bioengineering

authors： Nunes AS,Barros AS,Costa EC,Moreira AF,Correia IJ

更新日期：2019-01-01 00:00:00

abstract：:More than 80 years after its discovery, penicillin is still a widely used and commercially highly important antibiotic. Here, we analyse the metabolic network of penicillin synthesis in Penicillium chrysogenum based on the concept of elementary flux modes. In particular, we consider the synthesis of the invariant mole...

journal_title：Biotechnology and bioengineering

authors： Prauße MT,Schäuble S,Guthke R,Schuster S

更新日期：2016-01-01 00:00:00

abstract：:Genome-scale flux analysis of Escherichia coli DH5alpha growth in a complex medium was performed to investigate the relationship between the uptake of various nutrients and their metabolic outcomes. During the exponential growth phase, we observed a sequential consumption order of serine, aspartate and glutamate in th...

journal_title：Biotechnology and bioengineering

authors： Selvarasu S,Ow DS,Lee SY,Lee MM,Oh SK,Karimi IA,Lee DY

更新日期：2009-02-15 00:00:00

abstract：:We investigated the role of green sulfur bacteria inlight-responsive electricity generation in microbial electrochemical cells (MXCs). We operated MXCs containing either monocultures or defined cocultures of previously enriched phototrophic Chlorobium and anode-respiring Geobacter under anaerobic conditions in the abs...

journal_title：Biotechnology and bioengineering

authors： Badalamenti JP,Torres CI,Krajmalnik-Brown R

更新日期：2014-02-01 00:00:00

abstract：:Two attractive features of ELISA are the specificity of antibody-antigen recognition and the sensitivity achieved by enzymatic amplification. This report describes the development of a non-enzymatic molecular recognition platform adaptable to point-of-care clinical settings and field detection of biohazardous material...

journal_title：Biotechnology and bioengineering

authors： Stone GP,Mernaugh R,Haselton FR

更新日期：2005-09-20 00:00:00

abstract：:Crossflow nanofiltration experiments were performed to investigate the factors influencing the removal of amino acids by a commercially available polymeric thin-film composite membrane. The removals of five monoprotic (Ala, Val, Leu, Gly, and Thr), one diprotic (Asp), and one dibasic (Arg) amino acids in a range of pe...

journal_title：Biotechnology and bioengineering

authors： Shim Y,Chellam S

更新日期：2007-10-01 00:00:00

abstract：:A new biomass productivity model was parameterized for Chlorella vulgaris using short-term (<30 min) oxygen productivities from algal microcosms exposed to 6 light intensities (20-420 W/m(2)) and 6 temperatures (5-42 °C). The model was then validated against experimental biomass productivities recorded in bench-scale ...

journal_title：Biotechnology and bioengineering

authors： Béchet Q,Chambonnière P,Shilton A,Guizard G,Guieysse B

更新日期：2015-05-01 00:00:00

abstract：:CO(2) partial pressure (pCO(2)) in industrial cell culture reactors may reach 150-200 mm Hg, which can significantly inhibit cell growth and recombinant protein production. The inhibitory effects of elevated pCO(2) at constant pH are due to a combination of the increases in pCO(2) and [HCO(-) (3)], per se, and the ass...

journal_title：Biotechnology and bioengineering

authors： deZengotita VM,Schmelzer AE,Miller WM

更新日期：2002-02-15 00:00:00

abstract：:We have studied the growth rate dependence of hepatitis B surface antigen (HBsAg) p24(s) monomer and lipoprotein particle synthesis produced in Saccharomyces cerevisiae using galactose-limited continuous culture. The hepatitis B virus S gene, which encodes the p24(s) monomer, is transcribed under the control of the GA...

journal_title：Biotechnology and bioengineering

authors： Fu J,VanDusen WJ,Kolodin DG,O'Keefe DO,Herber WK,George HA

更新日期：1996-03-05 00:00:00

abstract：:Polypeptides containing between 4 and 32 repeats of a resilin-inspired sequence AQTPSSYGAP, derived from the mosquito Anopheles gambiae, have been used as tags on recombinant fusion proteins. These repeating polypeptides were inspired by the repeating structures that are found in resilins and sequence-related proteins...

journal_title：Biotechnology and bioengineering

authors： Lyons RE,Elvin CM,Taylor K,Lekieffre N,Ramshaw JA

更新日期：2012-12-01 00:00:00

abstract：:A structured kinetic model describing growth, differentiation, and penicillin production in submerged Penicillium chrysogenum fermentations is reported. The filamentous hyphae are divided into four distinct regions on the basis of the activities and structure of hyphal compartments, viz., actively growing (mainly apic...

journal_title：Biotechnology and bioengineering

authors： Paul GC,Thomas CR

更新日期：1996-09-05 00:00:00

abstract：:The biosynthesis of invertase by Saccharomyces carlsbergensis LAM 1068 was studied in relation to its glucose effect at both unsteady and steady states of growth. Experimental correlations between the dilution rate and invertase specific activity (E/X) in chemostat cultures led to an optimum for the enzyme synthesis a...

journal_title：Biotechnology and bioengineering

authors： Toda K

更新日期：1976-08-01 00:00:00

abstract：:The role of computers in the monitoring and control of fermentation processes has increased steadfastly. The ultimate utility of the machines will not depend on the availability of online sensors but also on the availability of techniques that combine direct measurements, leading towards estimates of variable closely ...

journal_title：Biotechnology and bioengineering

authors： Spriet JA,Botterman J,Buyser DR,De Visscher PL,Vandamme EJ

更新日期：1982-07-01 00:00:00

abstract：:It is shown that the mass transfer resistance can significantly distort the linearity of the Lineweaver-Burk plot of the kinetic data for a microbial culture which forms aggregates. For small flocs, the linearity of the Lineweaver-Burk plot is largely retained, but a different slope and intercept will be obtained comp...

journal_title：Biotechnology and bioengineering

authors： Ngian KF,Lin SH,Martin WR

更新日期：1977-12-01 00:00:00

abstract：:An alpha-galactosidase and a beta-mannanase produced by the hyperthermophilic bacterium, Thermotoga neapolitana 5068 (TN5068), separately and together, were evaluated for their ability to hydrolyze guar gum in relation to viscosity reduction of guar-based hydraulic fracturing fluids used in oil and gas well stimulatio...

journal_title：Biotechnology and bioengineering

authors： McCutchen CM,Duffaud GD,Leduc P,Petersen AR,Tayal A,Khan SA,Kelly RM

更新日期：1996-10-20 00:00:00

abstract：:A two-stage bioreactor scheme was developed for the large-scale production of recombinant proteins using a genetically engineered baculovirus/insect cell system. The first bioreactor was employed for cell growth and the second for cell infection. Silkworm Bm5 cells were infected with a recombinant baculovirus, BmNPV/P...

journal_title：Biotechnology and bioengineering

authors： Zhang J,Kalogerakis N,Behie LA,Iatrou K

更新日期：1993-07-01 00:00:00

abstract：:Overexpression of the cyclin-dependent kinase inhibitor p27 and exposure to low temperature (30 degrees C) represent two strategies to establish controlled proliferation processes for production of therapeutic proteins using Chinese hamster ovary (CHO) cells. Here we analyze the effect of growth inhibition on the qual...

journal_title：Biotechnology and bioengineering

authors： Kaufmann H,Mazur X,Marone R,Bailey JE,Fussenegger M

更新日期：2001-03-20 00:00:00

abstract：:In order to effectively treat obesity, it must be better understood at the cellular level with respect to metabolic state and environmental stress. However, current two-dimensional (2D) in vitro cell culture methods do not represent the in vivo adipose tissue appropriately due to the absence of complex architecture an...

journal_title：Biotechnology and bioengineering

authors： Turner PA,Harris LM,Purser CA,Baker RC,Janorkar AV

更新日期：2014-01-01 00:00:00

abstract：:Zwittermicin A (ZwA), a hybrid polyketide- peptide antibiotic, and N-acyl -homoserine-lactonase (AHL-lactonase), a kind of quorum-quenching enzyme, have the potential to prevent the virulence of Erwinia carotovora, which is a major pathogen of soft rot. The purpose of this study was to combine the two agents in one ho...

journal_title：Biotechnology and bioengineering

authors： Zhao C,Zeng H,Yu Z,Sun M

更新日期：2008-06-15 00:00:00

abstract：:Highly efficient antibody immobilization is extremely crucial for the development of high-performance polymeric microdevices for enzyme-linked immunosorbent assay (ELISA). In this article, a site-selective tyrosinase (TR)-catalyzed protein A strategy for antibody immobilization was developed to enhance the sensitivity...

journal_title：Biotechnology and bioengineering

authors： Yuan Y,He H,Lee LJ

更新日期：2009-02-15 00:00:00

abstract：:Enzymatic hydrolysis of cellulose for sugar production offers advantages of higher conversion, minimal by-product formation, low energy requirements, and mild operating conditions over other chemical conversions. The development of a kinetic model, based on observable, macroscopic properties of the overall system, is ...

journal_title：Biotechnology and bioengineering

authors： Wald S,Wilke CR,Blanch HW

更新日期：1984-03-01 00:00:00

abstract：:A cybernetic framework is presented which views microbial response in multiple substrate environments as a judicious investment of cellular resources in synthesizing different key proteins according to an optimal regulatory strategy. A mathematical model is developed within the cybernetic framework for the diauxic gro...

journal_title：Biotechnology and bioengineering

authors： Dhurjati P,Ramkrishna D,Flickinger MC,Tsao GT

更新日期：1985-01-01 00:00:00

abstract：:The relationship between local mass transfer coefficient and fluid velocity in heterogenous biofilms was investigated by combining microelectrodes and confocal scanning laser microscopy (CSLM). The biofilms were grown for up to 7 days and consisted of cell clusters separated by interstitial channels. Mass transfer coe...

journal_title：Biotechnology and bioengineering

authors： Stoodley P,Yang S,Lappin-Scott H,Lewandowski Z

更新日期：1997-12-20 00:00:00

abstract：:The methanotrophic bacterium Methylococcus capsulatus is capable of assimilating methane and oxygen into protein-rich biomass, however, the diverse metabolism of the microorganism also allows for several undesired cometabolic side-reactions to occur. In this study, the ammonia cometabolism in Methylococcus capsulatus ...

journal_title：Biotechnology and bioengineering

authors： Petersen LAH,Lieven C,Nandy SK,Villadsen J,Jørgensen SB,Christensen I,Gernaey KV

更新日期：2019-11-01 00:00:00

abstract：:Hexanal is a key organoleptic element of green-note that is found in both fragrances and flavors. We report a novel process for the production of hexanal using immobilized enzyme templates extracted from different plant sources in combination with hollow-fiber ultrafiltration for in situ separation. Enzyme templates, ...

journal_title：Biotechnology and bioengineering

authors： Schade F,Thompson JE,Legge RL

更新日期：2003-11-05 00:00:00

abstract：:Mammalian cell lines for recombinant protein production need to maintain productivity over extended cultivation times. Long-term stability studies are time and resource intensive, but are widely performed to identify and eliminate unstable candidates during cell line development. Production instability of manufacturin...

journal_title：Biotechnology and bioengineering

authors： Osterlehner A,Simmeth S,Göpfert U

更新日期：2011-11-01 00:00:00