Steric and electrostatic interactions govern nanofiltration of amino acids.

abstract：:Two thermophilic bacteria, Thermus aquaticus ATCC 25104 and Thermus species ATCC 27978, were investigated for their abilities to degrade BTEX (benzene, toluene, ethylbenzene, and xylenes). Thermus aquaticus and the Thermus sp. were grown in a nominal medium at 70 degrees C and 60 degrees C, respectively, and resting c...

journal_title：Biotechnology and bioengineering

authors： Chen CI,Taylor RT

更新日期：1995-12-20 00:00:00

abstract：:Highly substituted polychlorinated biphenyls (PCBs) are known to be very resistant to aerobic biodegradation, particularly the initial attack by biphenyl dioxygenase. Functional evolution of the substrate specificity of biphenyl dioxygenase was demonstrated by DNA shuffling and staggered extension process (StEP) of th...

journal_title：Biotechnology and bioengineering

authors： Brühlmann F,Chen W

更新日期：1999-06-05 00:00:00

abstract：:We developed a kinetic model that describes a heterogeneous reaction system for the production of D-p-hydroxyphenylglycine from D,L-p-hydroxyphenyl-hydantoin using D-hydantoinase of Bacillus stearothermophilus SD1 and N-carbamoylase of Agrobacterium tumefaciens NRRL B11291. As a biocatalyst, whole cells with separatel...

journal_title：Biotechnology and bioengineering

authors： Park JH,Oh KH,Lee DC,Kim HS

更新日期：2002-06-30 00:00:00

abstract：:Supercritical fluid technology offers the possibility to produce dry powder formulations of biocompatible materials, overcoming the drawbacks of classical micronization processes. In this work, Supercritical Assisted Atomization (SAA) has been used to micronize alpha-cyclodextrin (alpha-CD) and hydroxypropyl-beta-cycl...

journal_title：Biotechnology and bioengineering

authors： Reverchon E,Antonacci A

更新日期：2006-07-05 00:00:00

abstract：:The effect of hydrogen and carbon dioxide partial pressure on the growth of the extremely thermophilic archaebacterium Pyrodictium brockii at 98 degrees C was investigated. Previous work with this bacterium has been done using an 80:20 hydrogen-carbon dioxide gas phase with a total pressure of 4 atm; no attempt has be...

journal_title：Biotechnology and bioengineering

authors： Parameswaran AK,Schicho RN,Soisson JP,Kelly RM

更新日期：1988-08-05 00:00:00

abstract：:This paper describes construction of a nanosensory device for amplified detection of biologically important amines as chemical signals. The device was inspired by a biological signal transduction system, and was fabricated on an artificial cell membrane through self-organization of the molecular components, such as a ...

journal_title：Biotechnology and bioengineering

authors： Sasaki Y,Shioyama Y,Tian WJ,Kikuchi J,Hiyama S,Moritani Y,Suda T

更新日期：2010-01-01 00:00:00

abstract：:Most biotherapeutic drugs are recombinant monoclonal antibodies which are mostly produced in monoclonal cell lines derived from Chinese hamster ovary (CHO) cells. Various clones expressing a monoclonal recombinant antibody were analyzed and a correlation of the antibody concentration and the relative mRNA level of cal...

journal_title：Biotechnology and bioengineering

authors： Kober L,Zehe C,Bode J

更新日期：2012-10-01 00:00:00

abstract：:Growth dynamics of Pseudomonas aeruginosa, Burkholderia cepacia, and Staphylococcus aureus in a batch and chemostat, were investigated as a laboratory model system for persistent infections in cystic fibrosis. Most species-specific enumeration methods for mixed cultures are laborious or only qualitative, and therefore...

journal_title：Biotechnology and bioengineering

authors： Schmidt JK,König B,Reichl U

更新日期：2007-03-01 00:00:00

abstract：:A chimeric Fab was expressed in Chinese hamster ovary cells under the control of the CMV promoter in a two-stage production process. Cells were first grown to 90% confluence at 37 degrees C in a proliferation phase, followed by a production phase at either 37 degrees C or 28 degrees C. Medium supplemented with serum a...

journal_title：Biotechnology and bioengineering

authors： Schatz SM,Kerschbaumer RJ,Gerstenbauer G,Kral M,Dorner F,Scheiflinger F

更新日期：2003-11-20 00:00:00

abstract：:Mass transfer is known to play a critical role in bioprocess performance and henceforth monitoring dissolved O2 (DO) and dissolved CO2 (dCO2 ) is of paramount importance. At bioreactor level these parameters can be monitored online and can be controlled by sparging air/oxygen or stirrer speed. However, traditional sma...

journal_title：Biotechnology and bioengineering

authors： Chopda VR,Holzberg T,Ge X,Folio B,Wong L,Tolosa M,Kostov Y,Tolosa L,Rao G

更新日期：2020-04-01 00:00:00

abstract：:Blood vessel development is critical for the continued growth and progression of solid tumors and, therefore, makes an attractive target for improving cancer therapy. Indeed, vascular-targeted therapies have been extensively explored but they have shown minimal efficacy as monotherapies. Combretastatin A4 (CA-4) is a ...

journal_title：Biotechnology and bioengineering

authors： Liu Y,Kim YJ,Siriwon N,Rohrs JA,Yu Z,Wanga P

更新日期：2018-06-01 00:00:00

abstract：:The Chinese hamster ovary (CHO) cell line producing interferon-gamma (IFN-gamma) exhibits a 2-fold increase in specific productivity when grown at 32 degrees C compared to 37 degrees C. Low temperature also causes growth arrest, meaning that the cell density is significantly lower at 32 degrees C, nutrients are consum...

journal_title：Biotechnology and bioengineering

authors： Fox SR,Patel UA,Yap MG,Wang DI

更新日期：2004-01-20 00:00:00

abstract：:Regioselectivity of glycosyltransferases offers an important means to overcome the limitations of chemical synthesis of small molecule glycosides. In this study we explore a large multigene family of UDP-glucose:glycosyltransferases of Arabidopsis for their potential as novel biocatalysts for in vitro synthesis and wh...

journal_title：Biotechnology and bioengineering

authors： Lim EK,Ashford DA,Hou B,Jackson RG,Bowles DJ

更新日期：2004-09-05 00:00:00

abstract：:The enzymatic synthesis of polyesters from activated diesters and diols has been investigated. Differences between enzymatic synthesis and traditional chemical condensation processes are discussed. The disappearance of monomers during the initial phase of reaction indicates that enzyme has a higher specificity for tra...

journal_title：Biotechnology and bioengineering

authors： Chaudhary AK,Beckman EJ,Russell AJ

更新日期：1997-07-05 00:00:00

abstract：:Two instruments were developed to mechanize the handling of anaerobic microorganisms for microbial mutant isolation. The instruments automatically dispense liquid or agar medium to large and small 96-well platesand petridishes. Protocols were developed for inoculating different microorganisms, and calibration curves o...

journal_title：Biotechnology and bioengineering

authors： Stieglitz B,Defelice CP

更新日期：1986-09-01 00:00:00

abstract：:A sucrose utilization pathway was established in Ralstonia eutropha NCIMB11599 and R. eutropha 437-540 by introducing the Mannheimia succiniciproducens MBEL55E sacC gene that encodes β-fructofuranosidase. These engineered strains were examined for the production of poly(3-hydroxybutyrate) [P(3HB)] and poly(3-hydroxybu...

journal_title：Biotechnology and bioengineering

authors： Park SJ,Jang YA,Noh W,Oh YH,Lee H,David Y,Baylon MG,Shin J,Yang JE,Choi SY,Lee SH,Lee SY

更新日期：2015-03-01 00:00:00

abstract：:The effects of external calcium concentrations on biosynthesis of ginsenoside Rb1 and several calcium signal sensors were quantitatively investigated in suspension cultures of Panax notoginseng cells. It was observed that the synthesis of intracellular ginsenoside Rb1 in 3-day incubation was dependent on the medium Ca...

journal_title：Biotechnology and bioengineering

authors： Yue CJ,Zhong JJ

更新日期：2005-02-20 00:00:00

abstract：:Lipases are versatile biocatalists showing multiple applications in a wide range of biotechnological processes. The gene lipA coding for Lipase A from Bacillus subtilis was isolated by PCR amplification, cloned and expressed in Escherichia coli, Saccharomyces cerevisiae and Bacillus subtilis strains, using pBR322, YEp...

journal_title：Biotechnology and bioengineering

authors： Sánchez M,Prim N,Rández-Gil F,Pastor FI,Diaz P

更新日期：2002-05-05 00:00:00

abstract：:A continuous viral inactivation (CVI) chamber has been designed to operate with acceptable residence time distribution (RTD) characteristics. However, altering the CVI's geometry and operation to accommodate the scale was not obvious. In this work, we elucidate the influence of Dean vortices and leverage the transitio...

journal_title：Biotechnology and bioengineering

authors： Brown MR,Orozco R,Coffman J

更新日期：2020-03-01 00:00:00

abstract：:In view of the biochemical reaction catalyzed by enzyme powder suspended in a water-insoluble organic solvent, an equation was derived to estimate the amount of water bound to the enzyme powder. With this equation, an apparent adsorption isotherm between free water (water freely dissolved in benzene) and bound water (...

journal_title：Biotechnology and bioengineering

authors： Yamane T,Kojima Y,Ichiryu T,Nagata M,Shimizu S

更新日期：1989-09-01 00:00:00

abstract：:The feasibility of anaerobic methanethiol (MT) degradation at elevated sodium concentrations was investigated in a mesophilic (30 degrees C) lab-scale upflow anaerobic sludge bed (UASB) reactor, inoculated with estuarine sediment originating from the Wadden Sea (The Netherlands). MT was almost completely degraded (>95...

journal_title：Biotechnology and bioengineering

authors： van Leerdam RC,de Bok FA,Lens PN,Stams AJ,Janssen AJ

更新日期：2007-09-01 00:00:00

abstract：:This article deals with the modeling of the oxygen transfer in an industrial airlift fermentor used for lactic yeast production on whey substrates. The purpose of this study was to improve the understanding of the interactions among the various parameters that govern the oxygen transfer phenomena in this type of ferme...

journal_title：Biotechnology and bioengineering

authors： Pigache S,Dhoms P,Trystram G

更新日期：1992-04-15 00:00:00

abstract：:A xenogeneic hollow fiber bioreactor utilizing collagen-entrapped dispersed hepatocytes has been developed as an extracorporeal bioartificial liver (BAL) for potential treatment of acute human fulminant hepatitis. Prolonged viability, enhanced liver-specific functions, and differentiated state have been observed in pr...

journal_title：Biotechnology and bioengineering

authors： Wu FJ,Friend JR,Lazar A,Mann HJ,Remmel RP,Cerra FB,Hu WS

更新日期：1996-10-05 00:00:00

abstract：:Lignin, one of the major components of lignocellulosic biomass, plays an inhibitory role on the enzymatic hydrolysis of cellulose. This work examines the role of lignin in pretreated hardwood, where extents of cellulose hydrolysis decrease, rather than increase with increasing severity of liquid hot water pretreatment...

journal_title：Biotechnology and bioengineering

authors： Ko JK,Kim Y,Ximenes E,Ladisch MR

更新日期：2015-02-01 00:00:00

abstract：:CTLA4 is a membrane receptor on cytotoxic T cells whose interaction with the B7 counterreceptor on B cells is important in alloantigen responses. Soluble recombinant human and murine CTLA4 were produced using either Chinese hamster ovary or NS-0 cell lines. Expression vectors were constructed containing the gene codin...

journal_title：Biotechnology and bioengineering

authors： Flesher AR,Marzowski J,Wang WC,Raff HV

更新日期：1995-06-05 00:00:00

abstract：:Exposure of Bacillus subtilis to a shear rate of 1,482/s leads to a rapid loss of cell viability after 10 h of growth. Biochemical and molecular evidences provided below strongly suggest that cell death under high shear results from an apoptosis-like process similar to that described in eukaryotes, with activation of ...

journal_title：Biotechnology and bioengineering

authors： Sahoo S,Rao KK,Suraishkumar GK

更新日期：2006-05-05 00:00:00

abstract：:The impact of process environment changes on process performance is one of the most crucial process safety issues when cultivating mammalian cells in a bioreactor. In contrast, directed shifting of process parameters can also be used as an optimization tool providing higher cell and product yields. Compared to other s...

journal_title：Biotechnology and bioengineering

authors： Trummer E,Fauland K,Seidinger S,Schriebl K,Lattenmayer C,Kunert R,Vorauer-Uhl K,Weik R,Borth N,Katinger H,Müller D

更新日期：2006-08-20 00:00:00

abstract：:The adsorption of cellulase on cellulose and a lignacious residue was examined by using cellulase from Trichoderma reesei, hardwood pretreated by dilute sulfuric acid under high pressure, and a lignacious residue prepared by a complete enzymatic hydrolysis of the pretreated wood. A significant amount of cellulase was ...

journal_title：Biotechnology and bioengineering

authors： Ooshima H,Burns DS,Converse AO

更新日期：1990-08-20 00:00:00

abstract：:Microcrystalline cellulose (10 g/L Avicel) was hydrolysed by two major cellulases, cellobiohydrolase I (CBH I) and endoglucanase II (EG II), of Trichoderma reesei. Two types of experiments were performed, and in both cases the enzymes were added alone and together, in equimolar mixtures. In time course studies the rea...

journal_title：Biotechnology and bioengineering

authors： Medve J,Karlsson J,Lee D,Tjerneld F

更新日期：1998-09-05 00:00:00

abstract：:The effects ensuing from the interaction between membrane-bound sarcosine dehydrogenase and the surrounding lipids as well as the effects of membrane fluidity were described in this study. A 25-fold activation was observed upon the reconstitution of the enzyme in bilayers of SUVs made of DMPC. The considerable decreas...

journal_title：Biotechnology and bioengineering

authors： Kheirolomoom A,Katoh S,Sada E,Yoshida K

更新日期：1991-04-15 00:00:00