Anaerobic methanethiol degradation in upflow anaerobic sludge bed reactors at high salinity (> or =0.5 M Na(+)).

abstract：:Point and 95% confidence interval estimates of the true growth yield and maintenance coefficient are reported for four pure cultures andtwo mixed cultures of Lactobacillus bulgaricus and Streptococcus thermophilus grown anaerobically on 3% nonfat dry milk using batch followed by fed batch culture. An improved method o...

journal_title：Biotechnology and bioengineering

authors： Oner MD,Erickson LE,Yang SS

更新日期：1986-06-01 00:00:00

abstract：:Embryonic stem cells (ESCs) can differentiate into all somatic cell types, but the development of effective strategies to direct ESC fate is dependent upon defining environmental parameters capable of influencing cell phenotype. ESCs are commonly differentiated via cell aggregates referred to as embryoid bodies (EBs),...

journal_title：Biotechnology and bioengineering

authors： Sargent CY,Berguig GY,Kinney MA,Hiatt LA,Carpenedo RL,Berson RE,McDevitt TC

更新日期：2010-02-15 00:00:00

abstract：:During continuous lipase-catalyzed oleic acid esterification by ethanol in n-hexane, the oleic acid conversion, initially at 95%, decreases to 20% after 2 h. This decrease is caused by the accumulation of the water produced in the course of the reaction in the packed-bed reactor (PBR). In order to improve the PBR effi...

journal_title：Biotechnology and bioengineering

authors： Colombié S,Tweddell RJ,Condoret JS,Marty A

更新日期：1998-11-05 00:00:00

abstract：:This article deals with the modeling of the oxygen transfer in an industrial airlift fermentor used for lactic yeast production on whey substrates. The purpose of this study was to improve the understanding of the interactions among the various parameters that govern the oxygen transfer phenomena in this type of ferme...

journal_title：Biotechnology and bioengineering

authors： Pigache S,Dhoms P,Trystram G

更新日期：1992-04-15 00:00:00

abstract：:Lipases are versatile biocatalists showing multiple applications in a wide range of biotechnological processes. The gene lipA coding for Lipase A from Bacillus subtilis was isolated by PCR amplification, cloned and expressed in Escherichia coli, Saccharomyces cerevisiae and Bacillus subtilis strains, using pBR322, YEp...

journal_title：Biotechnology and bioengineering

authors： Sánchez M,Prim N,Rández-Gil F,Pastor FI,Diaz P

更新日期：2002-05-05 00:00:00

abstract：:For in vitro liver replacement devices, such as packed bed bioreactors, to maintain the essential functions of the liver, they must at least successfully support hepatocytes, the parenchymal cell of the liver. In vivo, the liver is a major consumer of oxygen. Hence it is unsurprising that the limited transport distanc...

journal_title：Biotechnology and bioengineering

authors： Niu M,Clemens MG,Coger RN

更新日期：2008-04-15 00:00:00

abstract：:A general framework for a genetically structured model is presented. The framework allows description of the interactions in a system of regulatory and structural genes. The model assumes equilibrium kinetics for the binding of regulatory proteins to the promoter regions of the genes and includes the possible activati...

journal_title：Biotechnology and bioengineering

authors： Agger T,Nielsen J

更新日期：1999-01-01 00:00:00

abstract：:The specific monoclonal antibody productivity (q(Mab)) of a murine hybridoma (CC9C10) increased with incubation temperature in the range 33 degrees C to 39 degrees C. q(Mab) was constant at each temperature and was independent of the phase of culture. The q(Mab) increased 97% at 39 degrees C and decreased by 21% at 33...

journal_title：Biotechnology and bioengineering

authors： Barnabé N,Butler M

更新日期：1994-11-20 00:00:00

abstract：:The effects of ZnCl2 additions on a mercuric reductase, merA, ammonia monooxygenase, amoA, and hydroxylamine (NH2OH) oxidoreductase, hao, gene expression were examined in continuously cultured Nitrosomonas europaea cells. The reactor was operated for 85 days with a 6.9 d hydraulic retention time and with four successi...

journal_title：Biotechnology and bioengineering

authors： Radniecki TS,Semprini L,Dolan ME

更新日期：2009-02-01 00:00:00

abstract：:Cell lines derived from Trichoplusia ni (Tn) are widely used as hosts in the baculovirus-insect cell system (BICS). One advantage of Tn cell lines is they can produce recombinant proteins at higher levels than cell lines derived from other insects. However, Tn cell lines are persistently infected with an alphanodaviru...

journal_title：Biotechnology and bioengineering

authors： Maghodia AB,Geisler C,Jarvis DL

更新日期：2020-07-14 00:00:00

abstract：:In this study, an analysis of the transition between the inactive ("closed") and active ("open") conformations of Candida rugosa lipase in solution is performed using irreversible enzyme inhibitors, cyclic saligenin phosphates. It is shown that >90% inhibition of the enzyme activity toward water-soluble substrates (es...

journal_title：Biotechnology and bioengineering

authors： Turner NA,Needs EC,Khan JA,Vulfson EN

更新日期：2001-01-05 00:00:00

abstract：:The production of ethanol from carob pod extract by free and immobilized Saccharomyces cerevisiae cells in batch and fed-batch culture was investigated. Fed-batch culture proved to be a better fermentation system for the production of ethanol than batch culture. In fed-batch culture, both free and immobilized S. cerev...

journal_title：Biotechnology and bioengineering

authors： Roukas T

更新日期：1994-02-05 00:00:00

abstract：:The inhibitory effect of ethanol on yeast growth and fermentation has been studied for the strain Saccharomyces cerevisiae ATCC No. 4126 under anaerobic batch conditions. The results obtained reveal that there is no striking difference between the response of growth and ethanol fermentation. Two kinetic models are als...

journal_title：Biotechnology and bioengineering

authors： Luong JH

更新日期：1985-03-01 00:00:00

abstract：:Streamline Direct CST I is a new type of ion exchanger with multi-modal functional groups, specially designed for an expanded bed adsorption (EBA) process, which can capture directly the proteins from the high ionic strength feedstocks with a high binding capacity. In this study, an experimental study is carried out f...

journal_title：Biotechnology and bioengineering

authors： Li P,Xiu G,Mata VG,Grande CA,Rodrigues AE

更新日期：2006-08-20 00:00:00

abstract：:Proteolysis associated with recombinant protein expression in Chinese Hamster Ovary (CHO) cells has hindered the development of biologics including HIV vaccines. When expressed in CHO cells, the recombinant HIV envelope protein, gp120, undergoes proteolytic clipping by a serine protease at a key epitope recognized by ...

journal_title：Biotechnology and bioengineering

authors： Li SW,Yu B,Byrne G,Wright M,O'Rourke S,Mesa K,Berman PW

更新日期：2019-09-01 00:00:00

abstract：:This article describes a methodology that implements a Markov decision process (MDP) optimization technique in a real time fed-batch experiment. Biological systems can be better modeled under the stochastic framework and MDP is shown to be a suitable technique for their optimization. A nonlinear input/output model is ...

journal_title：Biotechnology and bioengineering

authors： Saucedo VM,Karim MN

更新日期：1997-07-20 00:00:00

abstract：:Cellulase production from lignocellulosic materials was studied in solid-state cultivation by both static and mixed techniques under nonaseptic conditions. The effects of fermentation conditions, such as moisture content, pH, temperature, and aeration, on cellulase production by Trichoderma harzianum using a mixture o...

journal_title：Biotechnology and bioengineering

authors： Deschamps F,Giuliano C,Asther M,Huet MC,Roussos S

更新日期：1985-09-01 00:00:00

abstract：:A priori knowledge of the dissolved oxygen (O2) concentration profile within a hepatic hollow fiber (HF) bioreactor is important in developing an effective bioartificial liver assist device (BLAD). O2 provision is limiting within HF bioreactors and we hypothesize that supplementing a hepatic HF bioreactor's circulatin...

journal_title：Biotechnology and bioengineering

authors： Sullivan JP,Gordon JE,Palmer AF

更新日期：2006-02-05 00:00:00

abstract：:Lipase from Pseudomonas fluorescens biotype I was immobilized by adsorption of anion exchange resin using glutaraldehyde to enhance the adsorption. The activity yield of the immobilized lipase was very low (below 1%) when lipase activity was measured using emulsion substrate. The activity yield was 10-70% when lipase ...

journal_title：Biotechnology and bioengineering

authors： Kosugi Y,Tanaka H,Tomizuka N

更新日期：1990-09-01 00:00:00

abstract：:A simple feeding strategy was developed and successfully employed for nutritional control in a 2-L fed-batch culture of hybridoma cells. A previously developed stoichiometric model for animal cell growth was used to design a supplemental medium for feeding. Undialyzed fetal bovine serum and trace metals (Fe(2+), SeO(3...

journal_title：Biotechnology and bioengineering

authors： Xie L,Wang DI

更新日期：1996-09-20 00:00:00

abstract：:The growth dynamics of a methanol utilizing bacterium, L3, in batch bioreactors were experimentally investigated. Formaldehyde, a key intermediate of methanol oxidation, is indicated to have a significant role in the complex batch growth behavior of L3. The intricate batch growth dynamics of many microorganisms can be...

journal_title：Biotechnology and bioengineering

authors： Agrawal P,Lim HC

更新日期：1984-11-01 00:00:00

abstract：:Galactose oxidase (E.C. 1.1.3.9) was covalently immobilized to chemically modified porous silica particles by reaction of the native enzyme with pendant benzoyl azide groups on the carrier. The enzyme loading on the carrier was 100-150 units per milliliter. The immobilized enzyme was incorporated into a hardware assem...

journal_title：Biotechnology and bioengineering

authors： Dahodwala SK,Weibel MK,Humphrey AE

更新日期：1976-12-01 00:00:00

abstract：:Retroviral-derived biopharmaceuticals (RV) target numerous therapeutic applications, from gene therapy to virus-like particle (rVLP)-based vaccines. During particle formation, beside the pseudotyped envelope proteins, RV can incorporate proteins derived from the virus producer cells (VPC). This may be detrimental by r...

journal_title：Biotechnology and bioengineering

authors： Rodrigues AF,Guerreiro MR,Santiago VM,Dalba C,Klatzmann D,Alves PM,Carrondo MJ,Coroadinha AS

更新日期：2011-11-01 00:00:00

abstract：:Aggregates of Escherichia coli confined within hollowfiber reactors were either formed in place by culturing cells within the reactors, or were prepared by injecting a cell suspension that had been concentrated by centrifugation. The effective diffusive permeability of an uncharged nonreacting tracer, nitrous oxide, w...

journal_title：Biotechnology and bioengineering

authors： Libicki SB,Salmon PM,Robertson CR

更新日期：1988-06-20 00:00:00

abstract：:The feasibility of continuous production of proteins in chemostat cultures of Bacillus subtilis was investigated. An expression system consisting of the bacterium B. subtilis BR151 carrying plasmid p602/19 was used. The plasmid contains the cat (chioramphenicol acetyltrans-ferase) gene downstream of a strong vegetativ...

journal_title：Biotechnology and bioengineering

authors： Vierheller C,Goel A,Peterson M,Domach MM,Ataai MM

更新日期：1995-09-05 00:00:00

abstract：:Actively growing Escherichia coli C600(pBR322), immobilized within the macroporous matrix of asymmetric-wall hollow-fiber membranes, has been propagated to extremely high densities, typically more than 10(12) cells/mL of accessible void volume, in some regions cells accounting for nearly 100% of the available macrovoi...

journal_title：Biotechnology and bioengineering

authors： Inloes DS,Smith WJ,Taylor DP,Cohen SN,Michaels AS,Robertson CR

更新日期：1983-11-01 00:00:00

abstract：:Unsaturated fatty acid alpha-butylglucoside esters were prepared by enzymatic esterification of alpha-butylglucoside in nonaqueous media. Conditions were firstly optimized using oleic acid as acyl group. Synthesis was possible in several solvents but the presence of water co-product in the medium limited the reaction ...

journal_title：Biotechnology and bioengineering

authors： Bousquet MP,Willemot RM,Monsan P,Boures E

更新日期：1999-06-20 00:00:00

abstract：:Phenols are important industrial chemicals, and because they can be volatile, also appear as air pollutants. We examined the potential of tyrosinase to react with the volatile phenol p-cresol. Three lines of evidence support the conclusion that volatile phenols react with tyrosinase and are coupled (i.e., chemisorbed)...

journal_title：Biotechnology and bioengineering

authors： Wu LQ,Chen T,Wallace KK,Vazquez-Duhalt R,Payne GF

更新日期：2001-12-01 00:00:00

abstract：:Dilute aqueous solutions of polymers released by marine phytoplankton (microalgae) were shown to effectively reduce drag in capillary pipe flow. Tests were performed in a capillary turbulent flow viscometer which extruded small samples under high pressures. In all, 22 species were screened, and the products of one chl...

journal_title：Biotechnology and bioengineering

authors： Ramus J,Kenney BE,Shaughnessy EJ

更新日期：1989-01-25 00:00:00

abstract：:A new method is presented to precipitate proteins and amino acids from reverse micelles by dehydrating the micelles with molecular sieves. Nearly complete precipitation is demonstrated for alpha-chymotrypsin, cytochromec, and trytophan from 2-ethylhexyl sodium sulfosuccinate (AOT)/isooctane/water reverse micelle solut...

journal_title：Biotechnology and bioengineering

authors： Gupta RB,Han CJ,Johnston KP

更新日期：1994-09-20 00:00:00