Abstract:
:Galactose oxidase (E.C. 1.1.3.9) was covalently immobilized to chemically modified porous silica particles by reaction of the native enzyme with pendant benzoyl azide groups on the carrier. The enzyme loading on the carrier was 100-150 units per milliliter. The immobilized enzyme was incorporated into a hardware assembly suitable for the determination of galactose or lactose concentrations in complex biological fluids. The prototype instrument as described is suitable for continuous, on-line monitoring or discrete sample analysis. Reaction conditions can be readily provided which maintain global first order kinetics within the reactor and strict linearity of the procedure over a wide range of sample concentrations. Auto-inactivation of the immobilized enzyme can be prevented by K3Fe(CN)6 and long-term reactor stability can be achieved by the periodic application of the reagent to the enzyme reactor in situ.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Dahodwala SK,Weibel MK,Humphrey AEdoi
10.1002/bit.260181204subject
Has Abstractpub_date
1976-12-01 00:00:00pages
1679-94issue
12eissn
0006-3592issn
1097-0290journal_volume
18pub_type
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