Correlation between anatomical characteristics of ethanol organosolv pretreated Buddleja davidii and its enzymatic conversion to glucose.

abstract：:A phenomenological model of biocide action against microbial biofilms was derived. Processes incorporated in the model include bulk flow in and out of a well-mixed reactor, transport of dissolved species into the biofilm, substrate consumption by bacterial metabolism, bacterial growth, advection of cell mass within th...

journal_title：Biotechnology and bioengineering

authors： Stewart PS,Hamilton MA,Goldstein BR,Schneider BT

更新日期：1996-02-20 00:00:00

abstract：:Esters were prepared by acylation of hydroxypropyl cellulose with fatty acid catalyzed by immobilized lipase from Candida antarctica in tert-butanol. The nature of the substrates used, the initial water activity of the system, and the molecular weight of the hydroxypropyl cellulose were investigated. Moreover, Fourier...

journal_title：Biotechnology and bioengineering

authors： Sereti V,Stamatis H,Pappas C,Polissiou M,Kolisis F

更新日期：2001-02-20 00:00:00

abstract：:Several synthetic metabolic pathways for butanol synthesis have been reported in Escherichia coli by modification of the native CoA-dependent pathway from selected Clostridium species. These pathways are all dependent on the O2 -sensitive AdhE2 enzyme from Clostridium acetobutylicum that catalyzes the sequential reduc...

journal_title：Biotechnology and bioengineering

authors： Pásztor A,Kallio P,Malatinszky D,Akhtar MK,Jones PR

更新日期：2015-01-01 00:00:00

abstract：:Uranium contamination is a serious concern at several sites motivating the development of novel treatment strategies such as the Geobacter-mediated reductive immobilization of uranium. However, this bioremediation strategy has not yet been optimized for the sustained uranium removal. While several reactive-transport m...

journal_title：Biotechnology and bioengineering

authors： Zhao J,Scheibe TD,Mahadevan R

更新日期：2011-07-01 00:00:00

abstract：:Malathion is one of the most widely used organophosphorus pesticides in the United States and developing countries. Herein, we enhanced the degradation rate of malathion starting with a phosphotriesterase PoOPHM2 while also considering thermostability. In the first step, iterative saturation mutagenesis at residues li...

journal_title：Biotechnology and bioengineering

authors： Luo XJ,Zhao J,Li CX,Bai YP,Reetz MT,Yu HL,Xu JH

更新日期：2016-11-01 00:00:00

abstract：:Current approaches for purifying plasmids from bacterial production systems exploit the physiochemical properties of nucleic acids in non-specific capture systems. In this study, an affinity system for plasmid DNA (pDNA) purification has been developed utilizing the interaction between the lac operon (lacO) sequence c...

journal_title：Biotechnology and bioengineering

authors： Forde GM,Ghose S,Slater NK,Hine AV,Darby RA,Hitchcock AG

更新日期：2006-09-05 00:00:00

abstract：:Multiparameter flow cytometric techniques developed in our laboratories have been used for the "at-line" study of fed-batch bacterial fermentations. These fermentations were done at two scales, production (20 m(3)) and bench (5 x 10(-3) m(3)). In addition, at the bench scale, experiments were undertaken where the diff...

journal_title：Biotechnology and bioengineering

authors： Hewitt CJ,Nebe-Von Caron G,Axelsson B,McFarlane CM,Nienow AW

更新日期：2000-11-20 00:00:00

abstract：:Continous culture experiments with the obligatory methanotroph, Methylosinus trichosporium OB3b, were conducted to study the whole-cell methane monooxygenase (MMO) and nitrogenase activities in a nitrate minimal salts medium under oxygen-limited conditions with methane as the carbone source. The important variables in...

journal_title：Biotechnology and bioengineering

authors： Shah NN,Park S,Taylor RT,Droege MW

更新日期：1992-09-01 00:00:00

abstract：:To investigate the effects of factors secreted by different cell lines on human monoclonal antibody (MAb) integrity, 600 mg of a human MAb, which specifically binds to human erythrocytes, were produced in a perfusion process. After purification by protein A affinity chromatography, the MAb was used for integrity testi...

journal_title：Biotechnology and bioengineering

authors： Ackermann M,Jäger V,Marx U

更新日期：1995-01-20 00:00:00

abstract：:Highly efficient antibody immobilization is extremely crucial for the development of high-performance polymeric microdevices for enzyme-linked immunosorbent assay (ELISA). In this article, a site-selective tyrosinase (TR)-catalyzed protein A strategy for antibody immobilization was developed to enhance the sensitivity...

journal_title：Biotechnology and bioengineering

authors： Yuan Y,He H,Lee LJ

更新日期：2009-02-15 00:00:00

abstract：:Streamline Direct CST I is a new type of ion exchanger with multi-modal functional groups, specially designed for an expanded bed adsorption (EBA) process, which can capture directly the proteins from the high ionic strength feedstocks with a high binding capacity. In this study, an experimental study is carried out f...

journal_title：Biotechnology and bioengineering

authors： Li P,Xiu G,Mata VG,Grande CA,Rodrigues AE

更新日期：2006-08-20 00:00:00

abstract：:This article demonstrates the feasibility of a novel process concept for the remediation of PCB contaminated soil. The proposed process consists of PCB extraction from soil using solid polymer beads, followed by biodegradation of the extracted PCBs in a solid-liquid two-phase partitioning bioreactor (TPPB), where PCBs...

journal_title：Biotechnology and bioengineering

authors： Rehmann L,Daugulis AJ

更新日期：2008-04-01 00:00:00

abstract：:It has long been established that UVC light is a very effective method for inactivating pathogens in a fluid, yet the application of UVC irradiation to modern biotechnological processes is limited by the intrinsic short penetration distance of UVC light in optically dense protein solutions. This experimental and numer...

journal_title：Biotechnology and bioengineering

authors： Ren Y,Crump CM,Mackley MM,Li Puma G,Reis NM

更新日期：2016-07-01 00:00:00

abstract：:Proteolysis associated with recombinant protein expression in Chinese Hamster Ovary (CHO) cells has hindered the development of biologics including HIV vaccines. When expressed in CHO cells, the recombinant HIV envelope protein, gp120, undergoes proteolytic clipping by a serine protease at a key epitope recognized by ...

journal_title：Biotechnology and bioengineering

authors： Li SW,Yu B,Byrne G,Wright M,O'Rourke S,Mesa K,Berman PW

更新日期：2019-09-01 00:00:00

abstract：:This work investigated the use of nuclear magnetic resonance (NMR) spectroscopy in combination with a mathematical model of an encapsulated cell system as a method for rapidly assessing the status of a pancreatic substitute. To validate this method, an in vitro experiment was performed in which the encapsulated cells ...

journal_title：Biotechnology and bioengineering

authors： Gross JD,Long RC Jr,Constantinidis I,Sambanis A

更新日期：2007-09-01 00:00:00

abstract：:The inhibitory effect of ethanol on yeast growth and fermentation has been studied for the strain Saccharomyces cerevisiae ATCC No. 4126 under anaerobic batch conditions. The results obtained reveal that there is no striking difference between the response of growth and ethanol fermentation. Two kinetic models are als...

journal_title：Biotechnology and bioengineering

authors： Luong JH

更新日期：1985-03-01 00:00:00

abstract：:A mathematical model of glycolysis in Saccharomyces cerevisiae is presented. The model is based on rate equations for the individual reactions and aims to predict changes in the levels of intra- and extracellular metabolites after a glucose pulse, as described in part I of this study. Kinetic analysis focuses on a tim...

journal_title：Biotechnology and bioengineering

authors： Rizzi M,Baltes M,Theobald U,Reuss M

更新日期：1997-08-20 00:00:00

abstract：:Metabolic engineering of isopropyl alcohol (IPA)-producing Escherichia coli strains was conducted along with 13 C-metabolic flux analysis (MFA). A metabolically engineered E. coli strain expressing the adc gene derived from Clostridium acetobutylicum and the IPADH gene from C. beijerinckii did not produce IPA during i...

journal_title：Biotechnology and bioengineering

authors： Okahashi N,Matsuda F,Yoshikawa K,Shirai T,Matsumoto Y,Wada M,Shimizu H

更新日期：2017-12-01 00:00:00

abstract：:The thermophilic fungus Sporotrichum cellulophilum was immobilized with nonwoven materials for cellulase production. The cellulose powder concentration in the medium was an important factor controlling cellulase production. When the cellulose powder concentration in the nonwoven materials was more than 4%, cellulase p...

journal_title：Biotechnology and bioengineering

authors： Tamada M,Kasai N,Kumakura M,Kaetsu I

更新日期：1986-08-01 00:00:00

abstract：:A metabolic shift from lactate production (LP) to net lactate consumption (LC) phenotype was observed in certain Chinese hamster ovary (CHO) cell lines during the implementation of a new chemically defined medium (CDM) formulation for antibody production. In addition, this metabolic shift typically leads to process pe...

journal_title：Biotechnology and bioengineering

authors： Luo J,Vijayasankaran N,Autsen J,Santuray R,Hudson T,Amanullah A,Li F

更新日期：2012-01-01 00:00:00

abstract：:Many enzymes or fluorescent proteins produced in Escherichia coli are enzymatically active or fluorescent respectively when deposited as inclusion bodies. The occurrence of insoluble but functional protein species with native-like secondary structure indicates that solubility and conformational quality of recombinant ...

journal_title：Biotechnology and bioengineering

authors： Martínez-Alonso M,García-Fruitós E,Villaverde A

更新日期：2008-12-15 00:00:00

abstract：:Bifidobacterium animalis ATCC 27536 represents among bifidobacteria a host-model for cloning experiments. The segregational and structural stabilities of a family of cloning vectors with different molecular weights but sharing a common core were studied in continuous fermentation of the hosting B. animalis without sel...

journal_title：Biotechnology and bioengineering

authors： González Vara A,Rossi M,Altomare L,Eikmanns B,Matteuzzi D

更新日期：2003-10-20 00:00:00

abstract：:Kluyveromyces marxianus is a promising nonconventional yeast for biobased chemical production due to its rapid growth rate, high TCA cycle flux, and tolerance to low pH and high temperature. Unlike Saccharomyces cerevisiae, K. marxianus grows on low-cost substrates to cell densities that equal or surpass densities in ...

journal_title：Biotechnology and bioengineering

authors： McTaggart TL,Bever D,Bassett S,Da Silva NA

更新日期：2019-07-01 00:00:00

abstract：:This study deals with the use of an upward gas/liquid slug flow to reduce tubular mineral membrane fouling. The injection of air into the feedstream is designed to create hydrodynamic conditions that destabilize the cake layer over the membrane surface inside the filtration module complex. Experimental study was carri...

journal_title：Biotechnology and bioengineering

authors： Mercier M,Maranges C,Fonade C,Lafforgue-Delorme C

更新日期：1998-04-05 00:00:00

abstract：:We show the design features of a membrane bioreactor based on pulsatile flow across dimpled membranes. Results show an enhanced mass transfer of air of at least five-fold magnitude as compared with flat membranes. An increased working volume form 20 mL to 120 mL reduced the k(L)A at a given Reynolds number because of ...

journal_title：Biotechnology and bioengineering

authors： Beeton S,Millward HR,Bellhouse BJ,Nicholson AM,Jenkins N,Knowles CJ

更新日期：1991-12-05 00:00:00

abstract：:A technology has been developed to achieve optimal attachment of adhesion-independent lymphocytes to microcarrier beads. The activation of T-lymphocytes by concanavalin A was tested under microgravity conditions in an experiment carried out in space during the first Spacelab Life Science Mission. Activation, measure...

journal_title：Biotechnology and bioengineering

authors： Bechler B,Cogoli A,Cogoli-Greuter M,Müller O,Hunzinger E,Criswell SB

更新日期：1992-10-20 00:00:00

abstract：:Detachment from biofilms was evaluated using a mixed culture biofilm grown on primary wastewater in a tube reactor. The growth of biofilms and the detachment of biomass from biofilms are strongly influenced by hydrodynamic conditions. In a long-term study, three biofilms were cultivated in a biofilm tube reactor. The ...

journal_title：Biotechnology and bioengineering

authors： Horn H,Reiff H,Morgenroth E

更新日期：2003-03-05 00:00:00

abstract：:The effective diffusivity of oxygen, D(e), in Ca-alginate and PVA-SbQ gels was measured using a two-chamber vessel with a membrane between the two chambers. The effect of cell density, C(c), on D(e) in Ca-alginate gels was studied. The effective diffusivity of oxygen decreased with increasing cell density, to C(c) = 1...

journal_title：Biotechnology and bioengineering

authors： Sun Y,Furusaki S,Yamauchi A,Ichimura K

更新日期：1989-06-05 00:00:00

abstract：:Power densities and oxidation-reduction potentials (ORPs) of MFCs containing a pure culture of Shewanella oneidensis MR-1 were compared to mixed cultures (wastewater inoculum) in cube shaped, 1-, 2-, and 3-bottle batch-fed MFC reactor configurations. The reactor architecture influenced the relative power produced by t...

journal_title：Biotechnology and bioengineering

authors： Watson VJ,Logan BE

更新日期：2010-02-15 00:00:00

abstract：:We have studied the growth rate dependence of hepatitis B surface antigen (HBsAg) p24(s) monomer and lipoprotein particle synthesis produced in Saccharomyces cerevisiae using galactose-limited continuous culture. The hepatitis B virus S gene, which encodes the p24(s) monomer, is transcribed under the control of the GA...

journal_title：Biotechnology and bioengineering

authors： Fu J,VanDusen WJ,Kolodin DG,O'Keefe DO,Herber WK,George HA

更新日期：1996-03-05 00:00:00