Yield, solubility and conformational quality of soluble proteins are not simultaneously favored in recombinant Escherichia coli.

abstract：:In this study, the effects of the electric charges and fields on the viability of airborne microorganisms were investigated. The electric charges of different magnitude and polarity were imparted on airborne microbial cells by a means of induction charging. The airborne microorganisms carrying different electric charg...

journal_title：Biotechnology and bioengineering

authors： Mainelis G,Górny RL,Reponen T,Trunov M,Grinshpun SA,Baron P,Yadav J,Willeke K

更新日期：2002-07-20 00:00:00

abstract：:As the therapeutic monoclonal antibody (mAb) market continues to grow, optimizing production processes is becoming more critical in improving efficiencies and reducing cost-of-goods in large-scale production. With the recent trends of increasing cell culture titers from upstream process improvements, downstream capaci...

journal_title：Biotechnology and bioengineering

authors： Dizon-Maspat J,Bourret J,D'Agostini A,Li F

更新日期：2012-04-01 00:00:00

abstract：:A set of eight closely related plasmid constructs carrying CI857-controlled recombinant genes has been used as a model to study plasmid stability in Escherichia coli, in the absence of antibiotic selection. Plasmid loss rates and relative interdivision times of plasmid-bearing cells and plasmid-free cells have been an...

journal_title：Biotechnology and bioengineering

authors： Corchero JL,Villaverde A

更新日期：1998-06-20 00:00:00

abstract：:The production of beta-galactosidase from the E. coli phoA-lacZ fusion gene was studied to compare the gene expression behavior of two cloning methods: insertion to multicopy plasmids and integration into host cell's chromosome. The chromosome-integrating strain showed more tight control of fusion gene expression leve...

journal_title：Biotechnology and bioengineering

authors： Shin PK,Seo JH

更新日期：1990-12-20 00:00:00

abstract：:A transient, two-culture model simulating methane production from biomass has been developed. The simulator, based partially on the work by Andrews and McCarty, is capable of calculating the hydrolysis products of several common organic materials, accommodating various substrate feeding modes, and simulating the trans...

journal_title：Biotechnology and bioengineering

authors： Kleinstreuer C,Poweigha T

更新日期：1982-09-01 00:00:00

abstract：:As part of an investigation into the use of biological nitrogen fixation for fertilizer ammonia production, continuous culture studies of respiration and nitrogen fixation in the aerobic bacteria Azotobacter vinelandii under oxygen-limited conditions were conducted. Respiration and growth rates followed Monod forms wi...

journal_title：Biotechnology and bioengineering

authors： Diluccio RC,Kirwan DJ

更新日期：1984-01-01 00:00:00

abstract：:Co-Immobilization of dextransucrase (DS) and dextranase (DN) into calcium alginate includes the co-entrapment of soluble DS and adsorbed DN. DS converts sucrose into dextran, which is the substrate for DN, so that isomalto-oligosaccharides (IMOs) are follow-up products of dextran hydrolysis. The boundary conditions fo...

journal_title：Biotechnology and bioengineering

authors： Erhardt FA,Kügler J,Chakravarthula RR,Jördening HJ

更新日期：2008-07-01 00:00:00

abstract：:A drug-releasing model compound based on photosensitive acrylated ortho-nitrobenzylether (o-NBE) moiety and fluorescein was synthesized to demonstrate photolysis as a mechanism for drug release. Release of this model compound from a hydrogel network can be controlled with light intensity (5-20 mW/cm(2)), exposure dura...

journal_title：Biotechnology and bioengineering

authors： Griffin DR,Patterson JT,Kasko AM

更新日期：2010-12-15 00:00:00

abstract：:Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate l...

journal_title：Biotechnology and bioengineering

authors： Henry MN,MacDonald MA,Orellana CA,Gray PP,Gillard M,Baker K,Nielsen LK,Marcellin E,Mahler S,Martínez VS

更新日期：2020-04-01 00:00:00

abstract：:Autoflocculation and bioflocculation are considered to be the most promising means for the economical harvesting of microalgae. We have therefore studied these phenomena with cultures of Scenedesmus obliquus produced during biological tertiary wastewater treatment. The quantity of extracellular polymers produced durin...

journal_title：Biotechnology and bioengineering

authors： Lavoie A,de la Noüe J

更新日期：1987-11-01 00:00:00

abstract：:A desirable feature of many therapeutic glycoprotein production processes is to maximize the final sialic acid content. In this study, the effect of applying a novel chemical analog of the sialic acid precursor N-acetylmannosamine (ManNAc) on the sialic acid content of cellular proteins and a model recombinant glycopr...

journal_title：Biotechnology and bioengineering

authors： Yin B,Wang Q,Chung CY,Bhattacharya R,Ren X,Tang J,Yarema KJ,Betenbaugh MJ

更新日期：2017-08-01 00:00:00

abstract：:The human monoclonal antibody (humAb) 2F5 is a potent candidate for immunotherapy of HIV-1. The xenohybridoma derived humAb 2F5 is of IgG3 kappa isotype. Generally, IgG1 isotype has a longer half-life (beta-clearance) in humans than IgG3. Therefore the isotype was switched to an IgG1 by ligation of the 2F5 heavy chain...

journal_title：Biotechnology and bioengineering

authors： Kunert R,Steinfellner W,Purtscher M,Assadian A,Katinger H

更新日期：2000-01-05 00:00:00

abstract：:CTLA4 is a membrane receptor on cytotoxic T cells whose interaction with the B7 counterreceptor on B cells is important in alloantigen responses. Soluble recombinant human and murine CTLA4 were produced using either Chinese hamster ovary or NS-0 cell lines. Expression vectors were constructed containing the gene codin...

journal_title：Biotechnology and bioengineering

authors： Flesher AR,Marzowski J,Wang WC,Raff HV

更新日期：1995-06-05 00:00:00

abstract：:A chimeric Fab was expressed in Chinese hamster ovary cells under the control of the CMV promoter in a two-stage production process. Cells were first grown to 90% confluence at 37 degrees C in a proliferation phase, followed by a production phase at either 37 degrees C or 28 degrees C. Medium supplemented with serum a...

journal_title：Biotechnology and bioengineering

authors： Schatz SM,Kerschbaumer RJ,Gerstenbauer G,Kral M,Dorner F,Scheiflinger F

更新日期：2003-11-20 00:00:00

abstract：:Optically pure compounds are important in the synthesis of fine chemicals. Using directed evolution of enzymes to obtain biocatalysts that can selectively produce high-value chiral chemicals is often time-, money-, and resource-intensive; traditional semi-rational designs based on structural data and docking experimen...

journal_title：Biotechnology and bioengineering

authors： Cheng Z,Peplowski L,Cui W,Xia Y,Liu Z,Zhang J,Kobayashi M,Zhou Z

更新日期：2018-03-01 00:00:00

abstract：:Biofilms commonly develop in flowing aqueous environments, where the flow causes the biofilm to deform. Because biofilm deformation affects the flow regime, and because biofilms behave as complex heterogeneous viscoelastic materials, few models are able to predict biofilm deformation. In this study, a phase-field (PF)...

journal_title：Biotechnology and bioengineering

authors： Li M,Matouš K,Nerenberg R

更新日期：2020-07-13 00:00:00

abstract：:We have used a high-throughput LC/MS/UV/ELSD method to rapidly determine the absolute quantity and purity of 42 organic compounds from seven lead discovery libraries. A general calibration curve generated from a different set of 42 compounds with seven different scaffolds was used in this analysis. We have also studie...

journal_title：Biotechnology and bioengineering

authors： Fang L,Pan J,Yan B

更新日期：2000-01-01 00:00:00

abstract：:Utilising whole cells of recombinant Escherichia coli K27 (pCYP102, pGEc47) containing active cytochrome P450BM-3 monooxygenase [E.C. 1. 14.14.1], multiple oxidations of saturated and unsaturated fatty acids were performed by the enzyme under conditions of excess oxygen. The amount of oxygen dissolved in the culture m...

journal_title：Biotechnology and bioengineering

authors： Schneider S,Wubbolts MG,Oesterhelt G,Sanglard D,Witholt B

更新日期：1999-08-05 00:00:00

abstract：:The purpose of this article is to provide a few concrete examples of the potential to acceptably reduce the scope of validation and qualification testing based on scientific justification for the specific area of microbial fermentation. The key areas explored include: autoclave operational qualification (OQ) testing, ...

journal_title：Biotechnology and bioengineering

authors： Junker B

更新日期：2001-07-05 00:00:00

abstract：:Among various routes for the biological hydrogen production, the NAD(P)H-dependent pentose phosphate (PP) pathway is the most efficient for the dark fermentation. Few studies, however, have focused on the glucose-6-phosphate 1-dehydrogenase, encoded by zwf, as a key enzyme activating the PP pathway. Although the gluco...

journal_title：Biotechnology and bioengineering

authors： Kim YM,Cho HS,Jung GY,Park JM

更新日期：2011-12-01 00:00:00

abstract：:Most biotherapeutic drugs are recombinant monoclonal antibodies which are mostly produced in monoclonal cell lines derived from Chinese hamster ovary (CHO) cells. Various clones expressing a monoclonal recombinant antibody were analyzed and a correlation of the antibody concentration and the relative mRNA level of cal...

journal_title：Biotechnology and bioengineering

authors： Kober L,Zehe C,Bode J

更新日期：2012-10-01 00:00:00

abstract：:A framework using material balances in metabolic pathways to study cellular metabolism is examined and the results are discussed. Rate measurements on extracellular compounds alone were found to be not always sufficient to validate proposed unique intracellular mechanisms. The conditions to delineate among candidate m...

journal_title：Biotechnology and bioengineering

authors： Niranjan SC,San KY

更新日期：1989-08-05 00:00:00

abstract：:The mean sizes and size distributions of air bubbles and viscous castor oil drops were studied in a salt-rich aqueous solution (medium), first separately, and then simultaneously as a three-phase system. The dispersion was created in a 150-mm-diameter stirred tank equipped with a Rushton turbine, and the sizes were me...

journal_title：Biotechnology and bioengineering

authors： Galindo E,Pacek AW,Nienow AW

更新日期：2000-07-20 00:00:00

abstract：:High-level expression of recombinant penicillin acylase (PAC) using the strong trc promoter system in Escherichia coli is frequently limited by the processing and folding of PAC precursors (proPAC) in the periplasm, resulting in physiological stress and inclusion body formation in this compartment. Periplasmic heat-sh...

journal_title：Biotechnology and bioengineering

authors： Wu MS,Pan KL,Chou CP

更新日期：2007-04-01 00:00:00

abstract：:Traditional biological treatment models are "deduced" from formal chemical kinetics or dynamics of pure microorganism cultures growth. The best formal models give reasonable approximations of the biological treatment model with an ecosystem adaptation (ESA model). The model presented here explains some features of the...

journal_title：Biotechnology and bioengineering

authors： Vavilin VA

更新日期：1983-06-01 00:00:00

abstract：:Intracellular events that take place during influenza virus replication in animal cells are well understood qualitatively. However, to better understand the complex interaction of the virus with its host cell and to quantitatively analyze the use of cellular resources for virion formation or the overall dynamic for th...

journal_title：Biotechnology and bioengineering

authors： Sidorenko Y,Reichl U

更新日期：2004-10-05 00:00:00

abstract：:The hollow fiber enzyme reactor with pulsation developed by Kim and Chang (1983) was operated in a differential mode by recycling a substrate solution, in order to assess the efficiency of ultrafiltration swing. The rates of lactose conversion by beta-galactosidase contained in the shell side of the reactor were measu...

journal_title：Biotechnology and bioengineering

authors： Park TH,Kim IH,Chang HN

更新日期：1985-08-01 00:00:00

abstract：:Hyperacute rejection following xenogeneic transplantation in primates is mediated by naturally occurring IgM antibodies, which are specifically directed to alpha-Galactosyl residues on many nonprimate mammalian cells. Current approaches to remove these anti-alphaGal IgM include plasmapheresis followed by immunoaffinit...

journal_title：Biotechnology and bioengineering

authors： Karoor S,Molina J,Buchmann CR,Colton C,Logan JS,Henderson LW

更新日期：2003-01-20 00:00:00

abstract：:An alpha-galactosidase and a beta-mannanase produced by the hyperthermophilic bacterium, Thermotoga neapolitana 5068 (TN5068), separately and together, were evaluated for their ability to hydrolyze guar gum in relation to viscosity reduction of guar-based hydraulic fracturing fluids used in oil and gas well stimulatio...

journal_title：Biotechnology and bioengineering

authors： McCutchen CM,Duffaud GD,Leduc P,Petersen AR,Tayal A,Khan SA,Kelly RM

更新日期：1996-10-20 00:00:00

abstract：:Detachment from biofilms was evaluated using a mixed culture biofilm grown on primary wastewater in a tube reactor. The growth of biofilms and the detachment of biomass from biofilms are strongly influenced by hydrodynamic conditions. In a long-term study, three biofilms were cultivated in a biofilm tube reactor. The ...

journal_title：Biotechnology and bioengineering

authors： Horn H,Reiff H,Morgenroth E

更新日期：2003-03-05 00:00:00