Abstract:
:Bifidobacterium animalis ATCC 27536 represents among bifidobacteria a host-model for cloning experiments. The segregational and structural stabilities of a family of cloning vectors with different molecular weights but sharing a common core were studied in continuous fermentation of the hosting B. animalis without selective pressure. The rate of plasmid loss (R) and the specific growth rate difference (delta mu) between plasmid-free and plasmid-carrying cells were calculated for each plasmid and their relationship with plasmid size was studied. It was observed that both R and the numerical value of delta mu increased exponentially with plasmid size. The exponential functions correlating the specific growth rate difference and the rate of plasmid loss with the plasmid molecular weight were determined. Furthermore, the smallest of the plasmids studied, pLAV (4.3-kb) was thoroughly characterized by means of its complete nucleotide sequence. It was found that it contained an extra DNA fragment, the first bifidobacterial insertion sequence characterised, named IS 1999.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
González Vara A,Rossi M,Altomare L,Eikmanns B,Matteuzzi Ddoi
10.1002/bit.10737subject
Has Abstractpub_date
2003-10-20 00:00:00pages
145-50issue
2eissn
0006-3592issn
1097-0290journal_volume
84pub_type
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