High-throughput screening of chromatographic separations: IV. Ion-exchange.

abstract：:The surface hydrophobicity of a protein is an important factor for its interactions in solution and thus the outcome of its production process. Yet most of the methods are not able to evaluate the influence of these hydrophobic interactions under natural conditions. In the present work we have established a high resol...

journal_title：Biotechnology and bioengineering

authors： Amrhein S,Bauer KC,Galm L,Hubbuch J

更新日期：2015-12-01 00:00:00

abstract：:Based on batch cultivation data for the production of L-glutamic acid from glucose, a comparative evaluation was made for repeated batch cultivations using one and more fermentors. The problem was formulated as maximizing the productivity of metabolic product with the specified conversion with respect to the cell age ...

journal_title：Biotechnology and bioengineering

authors： Shimizu K,Kobayashi T,Nagara A,Matsubara M

更新日期：1985-05-01 00:00:00

abstract：:Both the forward and backward reactions of xylose isomerase (Sweetzyme Q) with xylose and glucose as substrates have been studied in terms of kinetics and thermodynamics. The relationship between the two reactions can thus be determined. Much attention has been given to the reaction with xylose as substrate. The optim...

journal_title：Biotechnology and bioengineering

authors： Olivier SP,du Toit PJ

更新日期：1986-05-01 00:00:00

abstract：:In vitro engineering systems can be powerful tools for studying tissue development in response to biophysical stimuli as well as for evaluating the functionality of engineered tissue grafts. It has been challenging, however, to develop systems that adequately integrate the application of biomimetic mechanical strain t...

journal_title：Biotechnology and bioengineering

authors： Cook CA,Huri PY,Ginn BP,Gilbert-Honick J,Somers SM,Temple JP,Mao HQ,Grayson WL

更新日期：2016-08-01 00:00:00

abstract：:The reporter bacterium, Pseudomonas fluorescens HK44 (HK44), was characterized in an immobilized state to investigate utility for deployment as a remote sensor in the subsurface. A packed-bed reactor with alginate-immobilized HK44 simulated hydrodynamic conditions such as might be found in a subsurface environment. Th...

journal_title：Biotechnology and bioengineering

authors： Webb OF,Bienkowski PR,Matrubutham U,Evans FA,Heitzer A,Sayler GS

更新日期：1997-06-05 00:00:00

abstract：:An algorithm for operating a fed-batch fermentor at an optimum specific fermentation rate is proposed. It does not require on-line measurement of nutrient concentration in the culture medium. An on-line estimate of the specific fermentation rate is sufficient for implementation of this scheme. The algorithm is model i...

journal_title：Biotechnology and bioengineering

authors： Agrawal P,Koshy G,Ramseier M

更新日期：1989-01-05 00:00:00

abstract：:In biotechnology and biomedicine reliable models of cell proliferation kinetics need to capture the relevant phenomena taking place during the mitotic cycle. To this aim, a novel mathematical model helpful to investigate the intrinsic kinetics of in vitro culture of adherent cells up to confluence is proposed in this ...

journal_title：Biotechnology and bioengineering

authors： Fadda S,Cincotti A,Cao G

更新日期：2012-03-01 00:00:00

abstract：:The direct microbial conversion (DMC) process for the production of ethanol from lignocellulosic biomass is limited by low volumetric ethanol production rates due to the low cell densities of Clostridium thermosaccharolyticum which is a key organism for ethanol production in this process. Hence, this study focuses on ...

journal_title：Biotechnology and bioengineering

authors： Mistry FR,Cooney CL

更新日期：1989-12-05 00:00:00

abstract：:We report the isolation of mutant strains of the methylotrophic yeast Hansenula polymorpha that are able to efficiently oxidize ethanol to acetaldehyde in an intact cell system. The oxidation reaction is catalyzed by alcohol oxidase (AOX), a key enzyme in the methanol metabolic pathway that is typically present only i...

journal_title：Biotechnology and bioengineering

authors： Moroz OM,Gonchar MV,Sibirny AA

更新日期：2000-04-05 00:00:00

abstract：:Proteinaceous organic wastes are suitable substrates to produce high added-value products in anaerobic mixed-culture fermentations. In these processes, the stoichiometry of the biotransformation depends highly on operational conditions such as pH or feeding characteristics and there are still no tools that allow the p...

journal_title：Biotechnology and bioengineering

authors： Regueira A,Lema JM,Carballa M,Mauricio-Iglesias M

更新日期：2020-01-01 00:00:00

abstract：:The mean sizes and size distributions of air bubbles and viscous castor oil drops were studied in a salt-rich aqueous solution (medium), first separately, and then simultaneously as a three-phase system. The dispersion was created in a 150-mm-diameter stirred tank equipped with a Rushton turbine, and the sizes were me...

journal_title：Biotechnology and bioengineering

authors： Galindo E,Pacek AW,Nienow AW

更新日期：2000-07-20 00:00:00

abstract：:Shikimic acid is one of several industrially interesting chiral starting materials formed in the aromatic amino acid pathway of plants and microorganisms. In this study, the physiology of a shikimic acid producing strain of Escherichia coli (derived from W3110) deleted in aroL (shikimic acid kinase II gene), was compa...

journal_title：Biotechnology and bioengineering

authors： Johansson L,Lindskog A,Silfversparre G,Cimander C,Nielsen KF,Lidén G

更新日期：2005-12-05 00:00:00

abstract：:In order to eliminate organic pollutants in waste gases, a biological filter bed technique has been developed, with a high self-regenerating capacity and a low pressure drop. The bed consists of an appropriate filling material (mainly peat compost), in order to let the microorganisms grow on the solid surface and to s...

journal_title：Biotechnology and bioengineering

authors： Ottengraf SP,Van Den Oever AH

更新日期：1983-12-01 00:00:00

abstract：:A phenomenological model of biocide action against microbial biofilms was derived. Processes incorporated in the model include bulk flow in and out of a well-mixed reactor, transport of dissolved species into the biofilm, substrate consumption by bacterial metabolism, bacterial growth, advection of cell mass within th...

journal_title：Biotechnology and bioengineering

authors： Stewart PS,Hamilton MA,Goldstein BR,Schneider BT

更新日期：1996-02-20 00:00:00

abstract：:The performance of an industrial pharmaceutical process (production of an active pharmaceutical ingredient by fermentation, API) was modeled by multiblock partial least squares (MBPLS). The most important process stages are inoculum production and API production fermentation. Thirty batches (runs) were produced accord...

journal_title：Biotechnology and bioengineering

authors： Lopes JA,Menezes JC,Westerhuis JA,Smilde AK

更新日期：2002-11-20 00:00:00

abstract：:N-linked glycan distribution affects important end-use characteristics such as the bioactivity and efficacy of many therapeutic proteins, (including monoclonal antibodies), in vivo. Yet, obtaining desired glycan distributions consistently during batch-to-batch production can be challenging for biopharmaceutical manufa...

journal_title：Biotechnology and bioengineering

authors： St Amand MM,Radhakrishnan D,Robinson AS,Ogunnaike BA

更新日期：2014-10-01 00:00:00

abstract：:The dissolved oxygen (DO) is an important variable in aerobic fermentations and affects the cell growth and product formation. Dissolved oxygen control is difficult in batch fermentations because of the time-varying conditions, time delays, and the probe dynamics. Modeling of the various patterns of biological activit...

journal_title：Biotechnology and bioengineering

authors： Sargantanis IG,Karim MN

更新日期：1998-10-05 00:00:00

abstract：:Tumor-targeted Salmonella VNP20009 preferentially replicate within tumor tissue and partially suppress tumor growth in murine tumor models. These Salmonella have the ability to locally induce apoptosis when they are in direct contact with cancer cells but they lack significant bystander killing, which may correlate wi...

journal_title：Biotechnology and bioengineering

authors： Quintero D,Carrafa J,Vincent L,Bermudes D

更新日期：2016-12-01 00:00:00

abstract：:Geotrichum candidum was cultivated at the surface of solid model media containing peptone to simulate the composition of Camembert cheese. The surface growth of G. candidum induced the diffusion of substrates from the core to the rind and the diffusion of produced metabolites from the rind to the core. In the range of...

journal_title：Biotechnology and bioengineering

authors： Aldarf M,Fourcade F,Amrane A,Prigent Y

更新日期：2004-07-05 00:00:00

abstract：:It is well established that salt enhances the interaction between solutes (e.g., proteins, displacers) and the weak hydrophobic ligands in hydrophobic interaction chromatography (HIC) and that various salts (e.g., kosmotropes, chaotropes, and neutral) have different effects on protein retention. In this article, the s...

journal_title：Biotechnology and bioengineering

authors： Xia F,Nagrath D,Garde S,Cramer SM

更新日期：2004-08-05 00:00:00

abstract：:The kinetics of precipitation of soya protein has been examined in a tubular flow reactor with the precipitants, ammonium sulfate, ethanol, divalent calcium, and sulfuric acid. The precipitate growth profiles obtained in all cases showed the rapid formation of a precipitate phase and the attainment of a final size wit...

journal_title：Biotechnology and bioengineering

authors： Chan MY,Hoare M,Dunnill P

更新日期：1986-03-01 00:00:00

abstract：:Hemicellulose residues can be hydrolyzed into a sugar syrup using dilute mineral acids. Although this syrup represents a potential feedstock for biofuel production, toxic compounds generated during hydrolysis limit microbial metabolism. Escherichia coli LY01, an ethanologenic biocatalyst engineered to ferment the mixe...

journal_title：Biotechnology and bioengineering

authors： Zaldivar J,Ingram LO

更新日期：1999-01-01 00:00:00

abstract：:In this article, we present the factors which influence the stability of the emulsion forming under the circumstances of the direct extraction of penicillin fermentation broth and the conditions of demulsification. A laboratory method has been developed for testing demulsifiers and a quantitative evaluation of the pro...

journal_title：Biotechnology and bioengineering

authors： Szabó C

更新日期：1992-06-20 00:00:00

abstract：:The design and operation of an industrial penicillin-V deacylation reactor is simulated, using a kinetic expression and mass transport parameters for the immobilized enzyme particles which were determined experimentally in a previous study. It is desirable to use a series of equalsized plug flow reactors with pH contr...

journal_title：Biotechnology and bioengineering

authors： Karlsen LG,Villadsen J

更新日期：1984-12-01 00:00:00

abstract：:The methanol-regulated alcohol oxidase promoter (PAOX1 ) of Pichia pastoris (syn. Komagataella spp. ) is one of the strongest promoters for heterologous gene expression. Although increasing the gene dosage is a common strategy to improve recombinant protein productivities, P. pastoris strains harboring more than two c...

journal_title：Biotechnology and bioengineering

authors： Cámara E,Monforte S,Albiol J,Ferrer P

更新日期：2019-07-01 00:00:00

abstract：:An extractive acetonobutylic fermentation process is developed by integrating bioproduction, Ultrafiltration, and distillation, providing simultaneous retention of biomass, selective removal of inhibitors from the permeate, as well as separation and purification of acetone-butanol-ethanol solvents. Successive batch fe...

journal_title：Biotechnology and bioengineering

authors： Minier M,Grateloup R,Blanc-Ferras E,Goma G

更新日期：1990-04-15 00:00:00

abstract：:A soil with aged contamination from lubricating oil (LO) and polychlorinated biphenyls (PCBs) was treated in a bioslurry reactor to investigate in-soil biosurfactant production by Pseudomonas aeruginosa, the most abundant indigenous, culturable, hydrocarbon-degrading microorganism. After 2 days of growth on LO, a depl...

journal_title：Biotechnology and bioengineering

authors： Hudak AJ,Cassidy DP

更新日期：2004-12-30 00:00:00

abstract：:Bioaffinity interactions have been, and continue to be, successfully adapted from nature for use in separation and detection applications. It has been previously reported that the magnetophoretic mobility of labeled cells show a saturation type phenomenon as a function of the concentration of the free antibody-magneti...

journal_title：Biotechnology and bioengineering

authors： Zhang H,Williams PS,Zborowski M,Chalmers JJ

更新日期：2006-12-05 00:00:00

abstract：:Membrane separation and chromatographic technologies are regarded as an attractive alternative to conventional academic small-scale ultracentrifugation procedures used for retrovirus purification. However, despite the increasing demands for purified retroviral vector preparations, new chromatography adsorbents with hi...

journal_title：Biotechnology and bioengineering

authors： Segura MM,Kamen A,Trudel P,Garnier A

更新日期：2005-05-20 00:00:00

abstract：:The feasibility of continuous production of proteins in chemostat cultures of Bacillus subtilis was investigated. An expression system consisting of the bacterium B. subtilis BR151 carrying plasmid p602/19 was used. The plasmid contains the cat (chioramphenicol acetyltrans-ferase) gene downstream of a strong vegetativ...

journal_title：Biotechnology and bioengineering

authors： Vierheller C,Goel A,Peterson M,Domach MM,Ataai MM

更新日期：1995-09-05 00:00:00