Metabolic modeling for predicting VFA production from protein-rich substrates by mixed-culture fermentation.

abstract：:Non-viral delivery of cell-impermeant drugs and DNA in vivo has traditionally relied upon either chemical or physical stress applied directly to target tissues. Physical methods typically use contact between an applicator, or electrode, and the target tissue and may involve patient discomfort. To overcome contact-depe...

journal_title：Biotechnology and bioengineering

authors： Connolly RJ,Lopez GA,Hoff AM,Jaroszeski MJ

更新日期：2009-12-01 00:00:00

abstract：:Numerous high-value proteins are secreted into the Escherichia coli periplasm by the General Secretory (Sec) pathway, but Sec-based production chassis cannot handle many potential target proteins. The Tat pathway offers a promising alternative because it transports fully folded proteins; however, yields have been too ...

journal_title：Biotechnology and bioengineering

authors： Browning DF,Richards KL,Peswani AR,Roobol J,Busby SJW,Robinson C

更新日期：2017-12-01 00:00:00

abstract：:A kinetic model for anaerobic digestion of insoluble solid-state substrates was developed. Rate equations for cell growth and substrate consumption were derived based on the assumption that the microorganisms assimilate the substrate mainly at the point of contact where they grow. The model emphasizes effects of subst...

journal_title：Biotechnology and bioengineering

authors： Huang SY,Chou MS

更新日期：1990-03-15 00:00:00

abstract：:An algorithm for operating a fed-batch fermentor at an optimum specific fermentation rate is proposed. It does not require on-line measurement of nutrient concentration in the culture medium. An on-line estimate of the specific fermentation rate is sufficient for implementation of this scheme. The algorithm is model i...

journal_title：Biotechnology and bioengineering

authors： Agrawal P,Koshy G,Ramseier M

更新日期：1989-01-05 00:00:00

abstract：:The feasibility of anaerobic methanethiol (MT) degradation at elevated sodium concentrations was investigated in a mesophilic (30 degrees C) lab-scale upflow anaerobic sludge bed (UASB) reactor, inoculated with estuarine sediment originating from the Wadden Sea (The Netherlands). MT was almost completely degraded (>95...

journal_title：Biotechnology and bioengineering

authors： van Leerdam RC,de Bok FA,Lens PN,Stams AJ,Janssen AJ

更新日期：2007-09-01 00:00:00

abstract：:In order to predict the potential benefit associated with mixing devices designed to introduce periodic light modulations in dense cultures of microalgae, it is necessary to develop a quantitative understanding of the relationship between the frequency of the modulations and the resulting photosynthetic efficiency enh...

journal_title：Biotechnology and bioengineering

authors： Terry KL

更新日期：1986-07-01 00:00:00

abstract：:In crude extract-based cell-free protein synthesis (CFPS), DNA templates are transcribed and translated into functional proteins. Although linear expression templates (LETs) are less laborious and expensive to generate, plasmid templates are often desired over polymerase chain reaction-generated LETs due to increased ...

journal_title：Biotechnology and bioengineering

authors： Zhu B,Gan R,Cabezas MD,Kojima T,Nicol R,Jewett MC,Nakano H

更新日期：2020-08-20 00:00:00

abstract：:In a previous paper, the overall or macrokinetics of the immobilized glucose oxidase--catalase system has been presented. In this paper a detailed analysis of the interaction of diffusion and reaction in this system will be presented. The mathematical treatment includes two consecutive reactions with two-substrate kin...

journal_title：Biotechnology and bioengineering

authors： Reuss M,Buchholz K

更新日期：1979-11-01 00:00:00

abstract：:It is well established that salt enhances the interaction between solutes (e.g., proteins, displacers) and the weak hydrophobic ligands in hydrophobic interaction chromatography (HIC) and that various salts (e.g., kosmotropes, chaotropes, and neutral) have different effects on protein retention. In this article, the s...

journal_title：Biotechnology and bioengineering

authors： Xia F,Nagrath D,Garde S,Cramer SM

更新日期：2004-08-05 00:00:00

abstract：:Copolymers of N-isopropylacrylamide and N-acryloyl amino acid spacers of varying chain length were synthesized. p-Aminobenzamidine (PABA) was chemically linked to the pendant carboxyl groups of these polymers to obtain thermoprecipitating affinity polymers. The inhibition constant (Ki) of these polymers for trypsin de...

journal_title：Biotechnology and bioengineering

authors： Vaidya AA,Lele BS,Kulkarni MG,Mashelkar RA

更新日期：1999-08-20 00:00:00

abstract：:In this study we describe a model that estimates the extracellular (nonfungal) and overall water contents of wheat grains during solid-state fermentation (SSF) with Aspergillus oryzae, using on-line measurements of oxygen, carbon dioxide, and water vapor in the gas phase. The model uses elemental balances to predict s...

journal_title：Biotechnology and bioengineering

authors： Nagel FJ,Tramper J,Bakker MS,Rinzema A

更新日期：2001-01-20 00:00:00

abstract：:Medium-chain-length-polyhydroxyalkanoic acids (MCL-PHAs) formed in Pseudomonas spp. have a rather broad distribution of monomer-units whose precursors are supplied via beta-oxidation degradation of MCL fatty acids fed as the carbon source and/or via PhaG enzyme catalyzing the acyl-group transfer from 3-hydroxyacyl-ACP...

journal_title：Biotechnology and bioengineering

authors： Choi MH,Xu J,Rho JK,Shim JH,Yoon SC

更新日期：2009-03-01 00:00:00

abstract：:The removal of substate molecules from aerobic microbial cultures is due to both consumption by microorganisms and stripping by the air stream. The air stripping component can be described by a constant parameter for low concentrations of volatile substrates. This air stripping parameter was found to have a value of 0...

journal_title：Biotechnology and bioengineering

authors： Singh N,Hill GA

更新日期：1987-09-01 00:00:00

abstract：:A model is proposed that accounts for the decreases in yield which occur in chemostat cultures of mesophilic yeasts at superoptimal growth temperatures. Two yield depressing effects were identified, one due to increased maintenance requirements by the viable fraction of the population, the other due to energy substrat...

journal_title：Biotechnology and bioengineering

authors： Van Uden N,Madeira-Lopes A

更新日期：1976-06-01 00:00:00

abstract：:Batch kinetic and inhibition studies were performed for the aerobic cometabolism of 1,1,1-trichloroethane (1,1,1-TCA), 1,1-dichloroethylene (1,1-DCE), and 1,1-dichloroethane (1,1-DCA) by a butane-grown mixed culture. These chlorinated aliphatic hydrocarbons (CAHs) are often found together as cocontaminants in groundwa...

journal_title：Biotechnology and bioengineering

authors： Kim Y,Arp DJ,Semprini L

更新日期：2002-12-05 00:00:00

abstract：:A combined approach based on the use of ATR-FT/IR and steady-state fluorescence spectroscopy allowed to shed light on the effects of the additive methoxypolyethylene glycol (MePEG) on the hydration, conformation and dynamic properties of lipase from Burkholderia cepacia dehydrated to form a film. Spectroscopic data sh...

journal_title：Biotechnology and bioengineering

authors： Secundo F,Barletta G,Mazzola G

更新日期：2008-10-01 00:00:00

abstract：:A comparative study of antimicrobial activity is done using three different electrospun nanofibers namely-CA, PAN, and PVC used as control and with various amounts of AgNO(3) being treated with UV-irradiation leading to the enhancement of silver nanoparticles. DMF is used as the common solvent which helps to undergo s...

journal_title：Biotechnology and bioengineering

authors： Lala NL,Ramaseshan R,Bojun L,Sundarrajan S,Barhate RS,Ying-Jun L,Ramakrishna S

更新日期：2007-08-15 00:00:00

abstract：:The effect of formate and hydrogen on isomerization and syntrophic degradation of butyrate and isobutyrate was investigated using a defined methanogenic culture, consisting of syntrophic isobutyrate-butyrate degrader strain IB, Methanobacterium formicicum strain T1N, and Methanosarcina mazeii strain T18. Formate and h...

journal_title：Biotechnology and bioengineering

authors： Wu WM,Jain MK,Hickey RF,Zeikus JG

更新日期：1996-11-05 00:00:00

abstract：:The in vitro generation of cell-based three dimensional (3D) nerve tissue is an attractive subject to improve graft survival and integration into host tissue for neural tissue regeneration or to model biological events in stem cell differentiation. Although 3D organotypic culture strategies are well established for 3D...

journal_title：Biotechnology and bioengineering

authors： Kim BC,Jun SM,Kim SY,Kwon YD,Choe SC,Kim EC,Lee JH,Kim J,Suh JF,Hwang YS

更新日期：2017-04-01 00:00:00

abstract：:Measurement of the surface roughness and thickness of biological films is laborious and usually destructive, thus hampering research in this area. We developed a laser triangulation sensor (LTS) set-up for the fast and nondestructive measurement of these biofilm parameters during growth. Using LTS measurements, the mo...

journal_title：Biotechnology and bioengineering

authors： Okkerse WJ,Ottengraf SP,Osinga-Kuipers B

更新日期：2000-12-20 00:00:00

abstract：:The end-product profile of the glucose fermentation by Propionibacterium freudenreichii ET-3 changed on an electrochemical treatment, in which the culture vessel was filled with a carbon felt anode. Acetate and propionate were produced as final end products in a molar ratio of 2:3 without any electrochemical treatment...

journal_title：Biotechnology and bioengineering

authors： Wang YF,Masuda M,Tsujimura S,Kano K

更新日期：2008-10-15 00:00:00

abstract：:Pancreatic islet transplantation is a promising alternative to whole-pancreas transplantation as a treatment of type 1 diabetes mellitus. This technique has been extensively developed during the past few years, with the main purpose of minimizing the complications arising from the standard protocols used in organ tran...

journal_title：Biotechnology and bioengineering

authors： Perez-Basterrechea M,Esteban MM,Vega JA,Obaya AJ

更新日期：2018-12-01 00:00:00

abstract：:This study addresses the characterization of human islet-like structures generated from a newly discovered sparse population of precursor cells (Petropavlovskaia and Rosenberg, 2002) in the human pancreas. These cells may be progenitor cells capable of producing pancreatic cells suitable for the treatment of type 1 di...

journal_title：Biotechnology and bioengineering

authors： Bodnar CA,Sen A,Kallos MS,Behie LA,Petropavlovskaia M,Rosenberg L

更新日期：2006-04-05 00:00:00

abstract：:The diffusion of viruses toward cells is a limiting step of the infection process. To be modeled correctly, this step must be evaluated in combination with the adsorption of the virus to the cell surface, which is a rapid but reversible step. In this paper, the recombinant adenovirus (rAd) diffusion and its adsorption...

journal_title：Biotechnology and bioengineering

authors： Gilbert PA,Kamen A,Bernier A,Garnier A

更新日期：2007-09-01 00:00:00

abstract：:This work investigated the use of nuclear magnetic resonance (NMR) spectroscopy in combination with a mathematical model of an encapsulated cell system as a method for rapidly assessing the status of a pancreatic substitute. To validate this method, an in vitro experiment was performed in which the encapsulated cells ...

journal_title：Biotechnology and bioengineering

authors： Gross JD,Long RC Jr,Constantinidis I,Sambanis A

更新日期：2007-09-01 00:00:00

abstract：:The patterning of cardiac myocytes on a micron scale ( approximately 5 microm) was achieved by microcontact printing of fibronectin onto a hydrophobically pretreated glass substrate. The patterned cardiac myocytes conjugated with each other by forming a gap junction, as judged from the synchronized Ca(2+) transition o...

journal_title：Biotechnology and bioengineering

authors： Kaji H,Takoh K,Nishizawa M,Matsue T

更新日期：2003-03-20 00:00:00

abstract：:Numerous studies on poly γ-d-glutamicacid (γ-PGA) production have investigated terrestrial renewable sources for reducing production costs, but there are no studies using waste marine resources so far. We aimed to develop a cost-effective production method of γ-d-PGA by Bacillus sp. SJ-10 using green macroalgae (Ulva ...

journal_title：Biotechnology and bioengineering

authors： Kim JH,Lee JM,Jang WJ,Park HD,Kim YO,Kim CH,Kong IS

更新日期：2019-07-01 00:00:00

abstract：:It is shown that the mass transfer resistance can significantly distort the linearity of the Lineweaver-Burk plot of the kinetic data for a microbial culture which forms aggregates. For small flocs, the linearity of the Lineweaver-Burk plot is largely retained, but a different slope and intercept will be obtained comp...

journal_title：Biotechnology and bioengineering

authors： Ngian KF,Lin SH,Martin WR

更新日期：1977-12-01 00:00:00

abstract：:We have developed a simple and robust transient expression system utilizing the 25 kDa branched cationic polymer polyethylenimine (PEI) as a vehicle to deliver plasmid DNA into suspension-adapted Chinese hamster ovary cells synchronized in G2/M phase of the cell cycle by anti-mitotic microtubule disrupting agents. The...

journal_title：Biotechnology and bioengineering

authors： Tait AS,Brown CJ,Galbraith DJ,Hines MJ,Hoare M,Birch JR,James DC

更新日期：2004-12-20 00:00:00

abstract：:This research focused on how the extent and type of primary solid-liquid separation can affect the performance of guard filtration and chromatography, in this instance hydrophobic interaction chromatography. The system used in the study was yeast (Saccharomyces cerevisiae) with the target molecule being an intracellul...

journal_title：Biotechnology and bioengineering

authors： Bracewell DG,Boychyn M,Baldascini H,Storey SA,Bulmer M,More J,Hoare M

更新日期：2008-08-01 00:00:00