Abstract:
:The end-product profile of the glucose fermentation by Propionibacterium freudenreichii ET-3 changed on an electrochemical treatment, in which the culture vessel was filled with a carbon felt anode. Acetate and propionate were produced as final end products in a molar ratio of 2:3 without any electrochemical treatments at the point of the consumption of lactate as an intermediate of the glucose fermentation. The ratio was changed to 1:1 at the point of the lactate consumption by the electrochemical incubation at an electrode potential of 0.4 V versus Ag|AgCl for 100 h. During further electrochemical incubation, propionate was oxidized to acetate as a final end-product in the microbe-containing anode chamber. 1,4-Dihydroxy-2-naphthoic acid produced by P. freudenreichii ET-3 itself would receive electrons from the metabolic pathway and serve as an electron transfer mediator from the microbial cells to the electrode.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Wang YF,Masuda M,Tsujimura S,Kano Kdoi
10.1002/bit.21914subject
Has Abstractpub_date
2008-10-15 00:00:00pages
579-86issue
3eissn
0006-3592issn
1097-0290journal_volume
101pub_type
杂志文章abstract::A newly designed batch calorimeter was used to investigate the degradability of some celluloses having varying degrees of crystallinity. The PTC of an aerobic culture of Cellulomonas uda ATCC 21399 obtained revealed a diauxic growth which is attributed to the presence of hemicellulose contaminating Avicel and MN300 ce...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270711
更新日期:1985-07-01 00:00:00
abstract::It is known that the rolA, rolB, and rolC genes of Agrobacterium rhizogenes T-DNA affect processes of plant development and activate the synthesis of secondary metabolites in transformed plant cells. Although a synergistic activity of the rol genes on root formation is well-documented, little is known about their indi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21727
更新日期:2008-05-01 00:00:00
abstract::The desired product of bioprocesses is often produced in particulate form, either as an inclusion body (IB) or as a crystal. Particle harvesting is then a crucial and attractive form of product recovery. Because the liquid phase often contains other bioparticles, such as cell debris, whole cells, particulate biocataly...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20885
更新日期:2006-07-05 00:00:00
abstract::Using (13)C-labeled glucose fed to a penicillin-overproducing strain of Penicillium chrysogenum, the intracellular fluxes were quantified, and the presence of two new pathways, not previously described in this organism, is suggested. Thus, glycine was synthesized not only by serine hydroxymethyltransferase, but also b...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:2000-06-20 00:00:00
abstract::Past technoeconomic modeling work has identified the relatively large contribution that enzymatic hydrolysis adds to the total cost of producing ethanol from lignocellulosic substrates. This cost was primarily due to the high concentration of enzyme and long incubation time that was required to obtain complete hydroly...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960820)51:4<375::AID-BIT
更新日期:1996-08-20 00:00:00
abstract::The study reports on the development of a bioreactor for the production of alpha-keto acids from D,L- or D-amino acids using Rhodotorula gracilis D-amino acid oxidase. D-amino acid oxidase was co-immobilized with catalase on Affi-Gel 10 matrix, and the reactor was operated as a continuous-stirred tank reactor (CSTR) o...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260441104
更新日期:1994-12-01 00:00:00
abstract::Ion-exchange (IEX) chromatography steps are widely applied in protein purification processes because of their high capacity, selectivity, robust operation, and well-understood principles. Optimization of IEX steps typically involves resin screening and selection of the pH and counterion concentrations of the load, was...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21905
更新日期:2008-08-01 00:00:00
abstract::Continuous propionic acid fermentations of lactate by Propionibacterium acidipropionici were studied in spiral wound fibrous bed bioreactors. Cells were imobilized by natural attachment to fiber surfaces and entrapment in the void volume within the fibrous matrix. A high cell density of approximately 37 g/L was attain...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260400404
更新日期:1992-08-05 00:00:00
abstract::In our previous study [Hong Y, Brown DG (2009) Appl Environ Microbiol 75(8):2346-2353], the adenosine triphosphate (ATP) level of adhered bacteria was observed to be 2-5 times higher than that of planktonic bacteria. Consequently, the proton motive force (Delta p) of adhered bacteria was approximately 15% greater than...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22606
更新日期:2010-04-01 00:00:00
abstract::Cost-effective production of biopharmaceuticals on a large scale can be carried out by perfusion cultures of mammalian cells. One problem with this mode of operation for submerged free-cell cultures is the requirement for an efficient cell separation device located in the effluent stream. The present work investigates...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19970505)54:3<239::AID-BIT
更新日期:1997-05-05 00:00:00
abstract::Hairy roots have the potential to produce a variety of valuable small and large molecules. The mist reactor is a gas phase bioreactor that has shown promise for low-cost culture of hairy roots. Using a newer, disposable culture bag, mist reactor performance was studied with two species, Artemisia annua L. and Arachis ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22892
更新日期:2010-12-01 00:00:00
abstract::Nitrous oxide (N2 O), a potent greenhouse gas, is reduced to N2 gas by N2 O-reducing bacteria (N2 ORB), a process which represents an N2 O sink in natural and engineered ecosystems. The N2 O sink activity by N2 ORB depends on temperature and O2 exposure, yet the specifics are not yet understood. This study explores th...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27654
更新日期:2020-12-11 00:00:00
abstract::This study demonstrates that masking the Feb regions of a monoclonal antibody (Mab) with synthetic antigens prior to covalent immobilization efficiency. Water-soluble adducts of poly(2-methyloxazoline) polymers and a synthetic-peptide epitope for the Mab were constructed. These synthetic antigens are referred to as Fa...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260391005
更新日期:1992-04-25 00:00:00
abstract::The S-System formalism provides a popular, versatile and mathematically tractable representation of metabolic pathways. At steady-state, after a logarithmic transformation, the S-System representation reduces into a system of linear equations. Thus, the maximization of a particular metabolite concentration or a flux s...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19971020)56:2<145::AID-BIT
更新日期:1997-10-20 00:00:00
abstract::We consider the application of high-throughput experimentation (HTE), including combinatorial methods, to catalyst discovery and early-phase optimization. While combinatorial- and parallel-testing methods have an already substantial history in catalysis, recent work by several groups promises significant efficiency ga...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(1998)61:4<203::aid-cc3>3.0
更新日期:1998-01-01 00:00:00
abstract::Microfiltration is an important unit operation in downstream processing. However, due to the influence of membrane fouling, prediction of the filtration performance for biological suspensions is difficult. This paper describes a modeling approach that allows a comprehensive description of filtration performance. On th...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19980720)59:2<189::aid-bit
更新日期:1998-07-20 00:00:00
abstract::The inhibitory effect of ethanol on yeast growth and fermentation has been studied for the strain Saccharomyces cerevisiae ATCC No. 4126 under anaerobic batch conditions. The results obtained reveal that there is no striking difference between the response of growth and ethanol fermentation. Two kinetic models are als...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260270311
更新日期:1985-03-01 00:00:00
abstract::Design of 3D scaffolds that can facilitate proper survival, proliferation, and differentiation of progenitor cells is a challenge for clinical applications involving large connective tissue defects. Cell migration within such scaffolds is a critical process governing tissue integration. Here, we examine effects of sca...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23027
更新日期:2011-05-01 00:00:00
abstract::Intracellular events that take place during influenza virus replication in animal cells are well understood qualitatively. However, to better understand the complex interaction of the virus with its host cell and to quantitatively analyze the use of cellular resources for virion formation or the overall dynamic for th...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章,评审
doi:10.1002/bit.20096
更新日期:2004-10-05 00:00:00
abstract::A two-parameter deactivation model is proposed to describe the kinetics of activity stabilization for some enzymes. The single-step unimolecular mechanism exhibits non-first-order deactivation kinetics since the final enzyme state, E(1) is not completely inactivated. The usefulness of the model is demonstrated by appl...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260300604
更新日期:1987-10-20 00:00:00
abstract::Polymeric membranes are increasingly used as supports for the immobilization of enzymes in bioreactors. One of the more common reactor types employed in lipase-catalyzed hydrolysis of oils, contains modified cellulose as a membrane material. We found that this type of material is readily attacked by cellulase present ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260400714
更新日期:1992-10-05 00:00:00
abstract::Surface plasmon resonance (SPR) technique was used to directly detect an intact form of insect pathogen: the baculovirus, Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio-functional layers was used to detect the intact AcMNPV: amine-reactive crosslinker with a disu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20882
更新日期:2006-07-05 00:00:00
abstract::A thermodynamic framework (UNIQUAC model with temperature dependent parameters) is applied to model the salt-induced protein crystallization equilibrium, i.e., protein solubility. The framework introduces a term for the solubility product describing protein transfer between the liquid and solid phase and a term for th...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990720)64:2<144::aid-bit
更新日期:1999-07-20 00:00:00
abstract::A novel strategy for the maximum production of a biodegradable copolymer, poly(3-hydroxybutyric-co-hydroxyvaleric) acid, P(HB-co-HV), was developed, based on the kinetic parameters obtained from fed-batch culture experiments of Alcaligenes eutrophus. The effects of various culture conditions such as mole ratio of carb...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1999-03-05 00:00:00
abstract::Temperature shifting was investigated as a means of improving cloned-gene product yields form a recombinant Escherichia coli containing the temperature-sensitive plasmid, pOU 140. In a series of shaker flask fermentations recombinant cells were thermally induced for different time periods. The growth, stability, and p...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260290416
更新日期:1987-03-01 00:00:00
abstract::The surface hydrophobicity of a protein is an important factor for its interactions in solution and thus the outcome of its production process. Yet most of the methods are not able to evaluate the influence of these hydrophobic interactions under natural conditions. In the present work we have established a high resol...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25677
更新日期:2015-12-01 00:00:00
abstract::The biosynthesis of invertase by Saccharomyces carlsbergensis LAM 1068 was studied in relation to its glucose effect at both unsteady and steady states of growth. Experimental correlations between the dilution rate and invertase specific activity (E/X) in chemostat cultures led to an optimum for the enzyme synthesis a...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260180807
更新日期:1976-08-01 00:00:00
abstract::Numerous biotechnological production processes are based on the submerse cultivation of filamentous fungi. Process design, however, is often hampered by the complex growth pattern of these organisms. In the morphologic development of coagulating filamentous fungi, like Aspergillus niger, conidial aggregation is the fi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21569
更新日期:2008-02-01 00:00:00
abstract::A flow cytometric kinetic study of hybridoma growth and monoclonal antibody production is presented, along with the influence of glutamine on intracellular responses such as (relative) cell size, and cell RNA and total protein content. Specific findings are: (1) RNA content remained constant throughout the growth phas...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260360109
更新日期:1990-06-05 00:00:00
abstract::Several researchers have demonstrated that the presence of a heterologous protein in recombinant Escherichia coli elicits a response similar to the heat-shock response, which includes enhanced protease expression. The present work detects, quantifies, and characterizes intracellular protease activity in E. coli that a...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260420602
更新日期:1993-09-05 00:00:00