Impact of clarification strategy on chromatographic separations: Pre-processing of cell homogenates.

Abstract:

:This research focused on how the extent and type of primary solid-liquid separation can affect the performance of guard filtration and chromatography, in this instance hydrophobic interaction chromatography. The system used in the study was yeast (Saccharomyces cerevisiae) with the target molecule being an intracellular protein; alcohol dehydrogenase (ADH). As expected, loading more poorly clarified suspensions (both centrates and primary filtrates) required proportionally larger guard filtration areas. In addition for feed suspensions prepared by centrifugation, increased clarification led to greater column capacity. However, where filtration was used to achieve similar clarification considerably lower column capacity was achieved. These results were attributed to centrifugation leading to the aggregation of lipids and their subsequent removal in this form before application to the column. Clarification by filtration leaves such lipids in their original "soluble" state and hence they are not removed. The importance of the need to examine such interactive effects in bioprocess studies is discussed. This observation was confirmed with further analytical work into the nature of the aggregated material formed in the supernatant under centrifugation conditions. This material was only soluble in an organic solvent, and identified as phophatidylcholine and ergosterol as among the components removed by centrifugation and guard filtration as opposed to filtration and guard filtration.

journal_name

Biotechnol Bioeng

authors

Bracewell DG,Boychyn M,Baldascini H,Storey SA,Bulmer M,More J,Hoare M

doi

10.1002/bit.21823

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

941-9

issue

5

eissn

0006-3592

issn

1097-0290

journal_volume

100

pub_type

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