Abstract:
:In a previous paper, the overall or macrokinetics of the immobilized glucose oxidase--catalase system has been presented. In this paper a detailed analysis of the interaction of diffusion and reaction in this system will be presented. The mathematical treatment includes two consecutive reactions with two-substrate kinetics. Furthermore, the deactivation of both enzymes due to the intermediate product peroxide is taken into account. The predicted results suggest that the efficiency of the glucose oxidase reaction depends on the concentration ranges of the two substrates. Furthermore, the external mass-transfer rate may cause a shift from glucose limitation to oxygen limitation. The efficiency of the coupled system is always higher than that predicted for the uncoupled reaction path. The calculations show that the economics of the coupled system depend a great deal on the deactivation of the enzymes.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Reuss M,Buchholz Kdoi
10.1002/bit.260211112subject
Has Abstractpub_date
1979-11-01 00:00:00pages
2061-81issue
11eissn
0006-3592issn
1097-0290journal_volume
21pub_type
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