Abstract:
:Bacterial magnetic particles (BMPs) were used for the identification of cyanobacterial DNA. Genus-specific oligonucleotide probes for the detection of Anabaena spp., Microcystis spp., Nostoc spp., Oscillatoria spp., and Synechococcus spp. were designed from the variable region of the cyanobacterial 16S rDNA of 148 strains. These oligonucleotide probes were immobilized on BMPs via streptavidin-biotin conjugation and employed for magnetic-capture hybridization against digoxigenin-labeled cyanobacterial 16S rDNA. Bacterial magnetic particles were magnetically concentrated, spotted in 100-microm-size microwell on MAG-microarray, and the fluorescent detection was performed. This work details the development of an automated technique for the magnetic isolation, the concentration of hybridized DNA, and the detection of specific target DNA on MAG-microarray. The entire process of hybridization and detection was automatically performed using a magnetic-separation robot and all five cyanobacterial genera were successfully discriminated.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Matsunaga T,Nakayama H,Okochi M,Takeyama Hdoi
10.1002/bit.1073subject
Has Abstractpub_date
2001-06-05 00:00:00pages
400-5issue
5eissn
0006-3592issn
1097-0290pii
10.1002/bit.1073journal_volume
73pub_type
杂志文章abstract::Rate and yield expressions relating to biomass and xanthan formation and to nitrogen, glucose, and oxygen consumption were established for Xanthomonas campestris batch fermentations in a bubble column. Microbial growth was described by the logistic rate equation, characterized by a maximum specific growth rate mu(M) =...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260330404
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260241115
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20328
更新日期:2005-02-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260171202
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260390113
更新日期:1992-01-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260290916
更新日期:1987-06-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26030
更新日期:2016-12-01 00:00:00
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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更新日期:1997-12-05 00:00:00
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journal_title:Biotechnology and bioengineering
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更新日期:2019-08-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20680
更新日期:2006-02-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990420)63:2<141::aid-bit
更新日期:1999-04-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21951
更新日期:2008-11-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990720)64:2<240::aid-bit
更新日期:1999-07-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25282
更新日期:2014-11-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260320111
更新日期:1988-06-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260381006
更新日期:1991-12-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20509
更新日期:2005-07-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:1999-01-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2010-02-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2007-09-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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更新日期:2013-02-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10521
更新日期:2003-03-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/1097-0290(20001220)70:6<699::aid-bit13>3.0
更新日期:2000-12-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260361104
更新日期:1990-12-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19990920)64:6<709::aid-bit
更新日期:1999-09-20 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25912
更新日期:2016-07-01 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:2001-02-05 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260290213
更新日期:1987-02-01 00:00:00