Growth of Trichosporon cutaneum under oxygen limitation: kinetics of oxygen uptake.

abstract：:Working conditions of a biochemical fuel cell formed by an oxygen cathode and a platinum bioanode in a Saccharomyces cerevisiae suspension metabolizing glucose are described. The biocell response in terms of bioanode potential and current drainage under different fermentation conditions is reported. A kinetic equation...

journal_title：Biotechnology and bioengineering

authors： Videla HA,Arvía AJ

更新日期：1975-10-01 00:00:00

abstract：:It has long been established that UVC light is a very effective method for inactivating pathogens in a fluid, yet the application of UVC irradiation to modern biotechnological processes is limited by the intrinsic short penetration distance of UVC light in optically dense protein solutions. This experimental and numer...

journal_title：Biotechnology and bioengineering

authors： Ren Y,Crump CM,Mackley MM,Li Puma G,Reis NM

更新日期：2016-07-01 00:00:00

abstract：:This research investigates the effect of solids retention time (SRT) on the acid-phase anaerobic digestion of primary sludge. A series of experiments were conducted using two continuous-flow 3-L units with the following configuration: a completely mixed reactor (CMR) with clarifier and solids recycle and an upflow ana...

journal_title：Biotechnology and bioengineering

authors： Elefsiniotis P,Oldham WK

更新日期：1994-06-05 00:00:00

abstract：:A laboratory-scale multiphase hollow fiber membrane reactor was employed to investigate the lipase-catalyzed enzymatic resolution of racemic glycidyl butyrate. A mathematical formulation was developed to simulate the performance of this system. Model parameters were determined independently (except the effective rate ...

journal_title：Biotechnology and bioengineering

authors： Wu DR,Cramer SM,Belfort G

更新日期：1993-04-25 00:00:00

abstract：:Glycolytic flux is increased and acetate production is reduced in Escherichia coli by the expression of heterologous NADH oxidase (NOX) from Streptococcus pneumoniae coupled with the deletion of the arcA gene, which encodes the ArcA regulatory protein. In this study, we examined the overproduction of a model recombina...

journal_title：Biotechnology and bioengineering

authors： Vemuri GN,Eiteman MA,Altman E

更新日期：2006-06-20 00:00:00

abstract：:To gain insight on the fate of arsenic (As) from drinking water treatment residuals in landfills, the mobilization of arsenate adsorbed onto granular ferric hydroxide (GFH) was studied in continuous anaerobic columns fed with a synthetic landfill leachate. The release of As was compared in biologically active and abio...

journal_title：Biotechnology and bioengineering

authors： Cortinas I,Sierra-Alvarez R,Field JA

更新日期：2008-12-15 00:00:00

abstract：:Fibrous proteins, such as silk, elastin and collagen are finding broad impact in biomaterial systems for a range of biomedical and industrial applications. Some of the key advantages of biosynthetic fibrous proteins compared to synthetic polymers include the tailorability of sequence, protein size, degradation pattern...

journal_title：Biotechnology and bioengineering

authors： Yigit S,Dinjaski N,Kaplan DL

更新日期：2016-05-01 00:00:00

abstract：:In this work the question was addressed if in nitrite-oxidizing activated sludge systems the environmental competition between Nitrobacter spp. and Nitrospira spp., which only recently has been discovered to play a role in these systems, is affected by the nitrite concentrations. Two parallel chemostats were inoculate...

journal_title：Biotechnology and bioengineering

authors： Nogueira R,Melo LF

更新日期：2006-09-05 00:00:00

abstract：:Water permeability (Lp), calculated from the volume variations of cells subjected to an osmotic shock, is classically used to characterize cell membrane properties. In this work, we have shown the importance of the kind of mixing reactor used to measure the Lp parameter. A mathematical model including the mixing time ...

journal_title：Biotechnology and bioengineering

authors： de Marañón IM,Gervais P,Molin P

更新日期：1997-10-05 00:00:00

abstract：:The effect of protein concentration in partitioning in PEG/salt aqueous two-phase systems has been investigated. PEG 4000/phosphate systems in the presence of 0% w/w and 8.8% w/w NaCl have been evaluated using amyloglucosidase, subtilisin, and trypsin inhibitor. Also, a PEG 4000/phosphate system with 3% w/w NaCl was u...

journal_title：Biotechnology and bioengineering

authors： Schmidt AS,Andrews BA,Asenjo JA

更新日期：1996-06-20 00:00:00

abstract：:We investigated the role of green sulfur bacteria inlight-responsive electricity generation in microbial electrochemical cells (MXCs). We operated MXCs containing either monocultures or defined cocultures of previously enriched phototrophic Chlorobium and anode-respiring Geobacter under anaerobic conditions in the abs...

journal_title：Biotechnology and bioengineering

authors： Badalamenti JP,Torres CI,Krajmalnik-Brown R

更新日期：2014-02-01 00:00:00

abstract：:A newly designed batch calorimeter was used to investigate the degradability of some celluloses having varying degrees of crystallinity. The PTC of an aerobic culture of Cellulomonas uda ATCC 21399 obtained revealed a diauxic growth which is attributed to the presence of hemicellulose contaminating Avicel and MN300 ce...

journal_title：Biotechnology and bioengineering

authors： Dermoun Z,Belaich JP

更新日期：1985-07-01 00:00:00

abstract：:The flotation of Streptomyces pilosus was studied in the absence and presence of adsorbed Pb(II) ions on the cell walls and with and without using sodium dodecylsulfate (SDS) as collector. Adsorption of SDS onto the organism in the absence and presence of Pb(II) ions was also investigated. It was found that in the abs...

journal_title：Biotechnology and bioengineering

authors： Sadowski Z,Golab Z,Smith RW

更新日期：1991-04-25 00:00:00

abstract：:The rationale behind KISR's involvement in SCP is explained and emphasis towards the use of aerobic thermophilic/thermotolerant methanol-utilizing bacterial cultures is stressed. An attempt is made to correlate different pilot plant harvesting procedures for biomass recovery with product quality. The latter is express...

journal_title：Biotechnology and bioengineering

authors： Prokop A,Ratcliffe HD,Fatayer MI,Al-Awadhi N,Khamis A,Murad M,Bond C,Hamdan IY

更新日期：1984-09-01 00:00:00

abstract：:In this study, we assessed the importance of cytoskeleton organization in the mammalian cells used to produce therapeutic proteins. Two cytoskeletal genes, Actin alpha cardiac muscle 1 (ACTC1) and a guanosine triphosphate GTPase-activating protein (TAGAP), were found to be upregulated in highly productive therapeutic ...

journal_title：Biotechnology and bioengineering

authors： Pourcel L,Buron F,Arib G,Le Fourn V,Regamey A,Bodenmann I,Girod PA,Mermod N

更新日期：2020-04-01 00:00:00

abstract：:The amount of ATP produced during n-alkane, fatty acid, or acetate metabolism in Candida tropicalis has been established from the P/O ratios measured on isolated mitochondria, yield on substrate and carbon balance. For these three kinds of substrates YATP value has been found to be close to 4, although Ysub on acetate...

journal_title：Biotechnology and bioengineering

authors： Gallo M,Azoulay E

更新日期：1975-12-01 00:00:00

abstract：:The removal of substate molecules from aerobic microbial cultures is due to both consumption by microorganisms and stripping by the air stream. The air stripping component can be described by a constant parameter for low concentrations of volatile substrates. This air stripping parameter was found to have a value of 0...

journal_title：Biotechnology and bioengineering

authors： Singh N,Hill GA

更新日期：1987-09-01 00:00:00

abstract：:Lipase from Candida cylindracea (CCL) was incorporated into polymerizable positively charged dialkylammonium bromide surfactant vesicles. The enzyme was incorporated by the use of the dehydration-rehydration method or by incubation. In the latter case, trapping efficiencies of up to 100% could be obtained. Activities ...

journal_title：Biotechnology and bioengineering

authors： Mosmuller EW,Franssen MC,Engbersen JF

更新日期：1993-06-20 00:00:00

abstract：:The initial steps of glycerol dissimilation and 1,3-propanediol (1, 3-PD) formation by Klebsiella pneumoniae anaerobically grown on glycerol were studied by quantifying the in vitro and in vivo activities of enzymes in continuous culture under conditions of steady state and oscillation and during transient phases. The...

journal_title：Biotechnology and bioengineering

authors： Ahrens K,Menzel K,Zeng A,Deckwer W

更新日期：1998-09-05 00:00:00

abstract：:Breast cancer cells experience a range of shear stresses in the tumor microenvironment (TME). However most current in vitro three-dimensional (3D) models fail to systematically probe the effects of this biophysical stimuli on cancer cell metastasis, proliferation, and chemoresistance. To investigate the roles of shear...

journal_title：Biotechnology and bioengineering

authors： Novak CM,Horst EN,Taylor CC,Liu CZ,Mehta G

更新日期：2019-11-01 00:00:00

abstract：:Urea, ammonium, and free amino acid contents were quantified in biological aging of a young wine under two flor film forming yeast strains, Saccharomyces cerevisiae race capensis and S. cerevisiae race bayanus, and compared. Cell viability in the film was different for the two yeast strains. Thus, capensis maintained ...

journal_title：Biotechnology and bioengineering

authors： Mauricio JC,Ortega JM

更新日期：1997-01-20 00:00:00

abstract：:Connexin-43 (Cx43) containing giant liposomes (GL) were prepared by a baculovirus expression-liposome fusion method. Recombinant budded viruses expressing Cx43 were prepared and then fused with GLs containing DOPG/DOPC at pH 4.5. Connexon formation on the GL membrane was observed by transmission electron microscope. H...

journal_title：Biotechnology and bioengineering

authors： Kamiya K,Tsumoto K,Arakawa S,Shimizu S,Morita I,Yoshimura T,Akiyoshi K

更新日期：2010-12-01 00:00:00

abstract：:This article develops and utilizes an in situ technique to estimate the Monod half-maximum rate concentration, K(s) and the maximum specific utilization rate constant, k, for biofilms. The technique employs a curve-matching method with kinetic results from several short-term experiments with completely mixed biofilm r...

journal_title：Biotechnology and bioengineering

authors： Rittmann BE,Crawford L,Tuck CK,Namkung E

更新日期：1986-11-01 00:00:00

abstract：:The high commercial values of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids have driven a strain-improvement program, aimed at increasing the content of those fatty acids in the microalga Pavlova lutheri (SMBA 60) as parent strain. After a round of mutation using UV-light as mutagenic agent, an isolate strain...

journal_title：Biotechnology and bioengineering

authors： Meireles LA,Guedes AC,Malcata FX

更新日期：2003-01-05 00:00:00

abstract：:Nanotechnology is becoming increasingly important for products used in our daily lives, such as the masses of titanium dioxide nanoparticle agglomerates (TiO(2) NPs) used in the pharmaceutical industry, for cosmetic products, or for pigments. Meanwhile, a serious lack of detailed information concerning the interaction...

journal_title：Biotechnology and bioengineering

authors： Chen P,Kanehira K,Sonezaki S,Taniguchi A

更新日期：2012-12-01 00:00:00

abstract：:The functional analysis of plant cells at the cellular and subcellular levels requires novel technologies for the directed manipulation of individual cells. In this report, we demonstrate the use of an infrared (1,064 nm) picosecond laser for the perforation of tobacco cell protoplasts. A single pulse was sufficient t...

journal_title：Biotechnology and bioengineering

authors： Schinkel H,Jacobs P,Schillberg S,Wehner M

更新日期：2008-01-01 00:00:00

abstract：:Buddleja davidii is a unique biomass that has many attractive agroenergy features, especially its wide range of growth habitat. The anatomical characteristics of B. davidii were investigated before and after ethanol organosolv pretreatment (one of the leading pretreatment technologies) in order to further understand t...

journal_title：Biotechnology and bioengineering

authors： Hallac BB,Ray M,Murphy RJ,Ragauskas AJ

更新日期：2010-12-01 00:00:00

abstract：:Abnormal airway mucus presents a significant challenge for inhalational drug delivery. Recognizing the thick and tenacious airway mucus seen in the cystic fibrosis (CF) patients as a critical barrier to effective drug delivery, both into the mucus layer itself as well as across that layer to the underlying airway epit...

journal_title：Biotechnology and bioengineering

authors： Yang Y,Tsifansky MD,Shin S,Lin Q,Yeo Y

更新日期：2011-06-01 00:00:00

abstract：:Static and dynamic characteristics of continuous cultures of recombinant methylotrophs, which are designed to improve the selectivity of plasmid-bearing cells and the plasmid stability, are investigated in detail. Operational regions in which coexistence (survival of plasmid-bearing and plasmid-free cells) operation i...

journal_title：Biotechnology and bioengineering

authors： Parulekar SJ,Chang YK,Modak JM,Lim HC

更新日期：1987-06-01 00:00:00

abstract：:James (Jay) E. Bailey was a pioneer in biotechnology and biochemical engineering. During his 30 years in academia he made seminal contributions to many fields of chemical engineering science, including catalysis and reaction engineering, bioprocess engineering, mathematical modeling of cellular processes, recombinant ...

journal_title：Biotechnology and bioengineering

authors： Dordick JS,Khosla C

更新日期：2002-09-05 00:00:00