Mannitol-guided delivery of Ciprofloxacin in artificial cystic fibrosis mucus model.

abstract：:The marine diatom Cyclotella cryptica was grown over a period of 13 months in a 48-m(2) shallow outdoor flume. The use of foil arrays at intervals of 1.2 m to effect systematic vertical mixing in the flume was found to significantly enhance microalgal production (p = 0.006). Average photosynthetic efficiencies (based ...

journal_title：Biotechnology and bioengineering

authors： Laws EA,Taguchi S,Hirata J,Pang L

更新日期：1988-07-05 00:00:00

abstract：:The application of the unscented Kalman filter to control starvation-induced programmed cell death-apoptosis-in Chinese hamster ovary cells was investigated. Neural network-based sensitivity analysis identified glutamine and asparagine as two major amino acids that play a key role in the suppression of apoptosis. Dyna...

journal_title：Biotechnology and bioengineering

authors： Simon L,Karim MN

更新日期：2002-06-20 00:00:00

abstract：:A gene coding for human parathyroid hormone (hPTH) was synthesized and cloned into a yeast expression and secretion vector containing the mating factor alpha pre-pro leader sequence and the galactose-inducible promoter, GAL10. The intact hPTH(1-84) was found to be secreted into the culture medium. As observed in the p...

journal_title：Biotechnology and bioengineering

authors： Chung BH,Park KS

更新日期：1998-01-20 00:00:00

abstract：:A thermodynamic model was used in this study to predict the adhesion of Phanerochaete chrysosporium INA-12 as conidiospores or mycelium to various solid carriers. Theoretical predictions were closely reflected by experimental results. Amount of immobilized mycelium was higher for hydrophobic (polypropylene and polyure...

journal_title：Biotechnology and bioengineering

authors： Asther M,Bellon-Fontaine MN,Capdevila C,Corrieu G

更新日期：1990-03-05 00:00:00

abstract：:We demonstrate a novel application of (13)C pulsed field gradient (PFG) NMR to monitor mass transfer, due to both flow and diffusion, in a 3D complex porous support structure modified by biofilm growth. This enables timescales an order of magnitude larger than previously possible to be accessed with respect to displac...

journal_title：Biotechnology and bioengineering

authors： Graf von der Schulenburg DA,Akpa BS,Gladden LF,Johns ML

更新日期：2008-10-15 00:00:00

abstract：:Mass balances were performed on an alkaline lysis operation for the primary recovery of supercoiled plasmid DNA as part of a process for plasmid gene preparation. Escherichia coli DH5alpha/pSVbeta was cultured in defined medium by fed-batch fermentation and harvested at the end of the exponential phase. Alkaline lysis...

journal_title：Biotechnology and bioengineering

authors： Ciccolini LA,Shamlou PA,Titchener-Hooker N

更新日期：2002-03-30 00:00:00

abstract：:The effect of hydrogen and carbon dioxide partial pressure on the growth of the extremely thermophilic archaebacterium Pyrodictium brockii at 98 degrees C was investigated. Previous work with this bacterium has been done using an 80:20 hydrogen-carbon dioxide gas phase with a total pressure of 4 atm; no attempt has be...

journal_title：Biotechnology and bioengineering

authors： Parameswaran AK,Schicho RN,Soisson JP,Kelly RM

更新日期：1988-08-05 00:00:00

abstract：:We consider the application of high-throughput experimentation (HTE), including combinatorial methods, to catalyst discovery and early-phase optimization. While combinatorial- and parallel-testing methods have an already substantial history in catalysis, recent work by several groups promises significant efficiency ga...

journal_title：Biotechnology and bioengineering

authors： Newsam JM,Schüth F

更新日期：1998-01-01 00:00:00

abstract：:Enzymatic bioreactors with both high flow characteristics and mechanical stability based on macroporous poly(2-vinyl-4, 4-dimethylazlactone-co-acrylamide-co-ethylene dimethacrylate) monoliths have been prepared. Covalent immobilization of trypsin on these support is achieved in a single reaction step using the azlacto...

journal_title：Biotechnology and bioengineering

authors： Xie S,Svec F,Fréchet JM

更新日期：1999-01-05 00:00:00

abstract：:In this report, alpha-Amylase originating from Bacillus subtilis (liquefying type) was immobilized on partially imidoesterized polyacrylonitrile (PAN) by covalent bonding. For the preparation of immobilized alpha-amylase, which has a high activity and high stability to repeated use, the optimum conditions for the prep...

journal_title：Biotechnology and bioengineering

authors： Handa T,Hirose A,Akino T,Watanabe K,Tsuchiya H

更新日期：1983-12-01 00:00:00

abstract：:Genetically engineered microbes have been intensively investigated as a means for the cost-effective production of isoprenoids. Bacillus subtilis is a promising microbial host for this purpose because of its fast growth rate and GRAS (generally regarded as safe) status. To date, development of this host has been impai...

journal_title：Biotechnology and bioengineering

authors： Zhou K,Zou R,Zhang C,Stephanopoulos G,Too HP

更新日期：2013-09-01 00:00:00

abstract：:In this study, the inhibitory effects and anaerobic biotransformation of acrylic acid in computer-controlled pH-stat completely stirred tank reactors (CSTRs) with two different cultures, namely unacclimated and acrylate-acclimated acetate-enriched Methanosarcina and homogenized (crushed) granular cultures, were invest...

journal_title：Biotechnology and bioengineering

authors： Demirer GN,Speece RE

更新日期：1999-01-20 00:00:00

abstract：:An immobilized enzyme reactor has been developed to remove heparin, the anticoagulant that is required in all extracorporeal devices for patients undergoing open-heart surgery or kidney dialysis. The device uses the enzyme heparinase (EC 4.2.2.7), which is covalently linked to agarose with cyanogen bromide. A critical...

journal_title：Biotechnology and bioengineering

authors： Bernstein H,Yang VC,Langer R

更新日期：1987-08-05 00:00:00

abstract：:We describe a novel procedure to estimate the net growth rate of biofilms on multiple substrates. The approach is based on diffusion-reaction mass balances for chemical species in a continuum biofilm model with reaction kinetics corresponding to a Double-Monod expression. This analytical model considers a heterogeneou...

journal_title：Biotechnology and bioengineering

authors： Gonzo EE,Wuertz S,Rajal VB

更新日期：2014-11-01 00:00:00

abstract：:The transient and steady-state responses of hybridoma growth and metabolism to glutamine pulse and step changes have been examined. Metabolic quotients are reported for oxygen, glucose, lactate, ammonia, glutamine, alanine, and other amino acids. The specific glutamine consumption rate increased rapidly after all glut...

journal_title：Biotechnology and bioengineering

authors： Miller WM,Wilke CR,Blanch HW

更新日期：1989-01-20 00:00:00

abstract：:The immobilization of dextransucrase in Ca-alginate beads relies on the close association between dextran polymer and dextransucrase. However, high amounts of dextran in the enzyme preparation drastically limit the specific activity of the immobilized enzyme (4 U/mL of alginate beads). Moreover, even in the absence of...

journal_title：Biotechnology and bioengineering

authors： Dols-Lafargue M,Willemot RM,Monsan PF,Remaud-Simeon M

更新日期：2001-11-05 00:00:00

abstract：:Polymers produced by horseradish-peroxidase-catalyzed coupling of phenols have been explored as potential substitutes for phenol-formaldehyde resins. To overcome low substrate solubilities and product molecular weights in water, enzymatic polymerizations in aqueous-organic mixtures have been examined. Peroxidase vigor...

journal_title：Biotechnology and bioengineering

authors： Dordick JS,Marletta MA,Klibanov AM

更新日期：1987-07-01 00:00:00

abstract：:A dynamic method is proposed for k(l)a measurement in aerated and agitated reactors, in which a change in the total pressure in the reactor by approximately 20% leads to a simultaneous change in the oxygen concentration in all the bubbles in the dispersion. This procedure suppresses the influence of nonideal mixing of...

journal_title：Biotechnology and bioengineering

authors： Linek V,Benes P,Vacek V

更新日期：1989-05-01 00:00:00

abstract：:Squalene is a lipophilic and non-volatile triterpene with many industrial applications for food, pharmaceuticals, and cosmetics. Metabolic engineering focused on optimization of the production pathway suffer from little success in improving titers because of a limited space of the cell membrane accommodating the lipop...

journal_title：Biotechnology and bioengineering

authors： Meng Y,Shao X,Wang Y,Li Y,Zheng X,Wei G,Kim SW,Wang C

更新日期：2020-07-21 00:00:00

abstract：:After separation by microscale non-denaturing two-dimensional gel electrophoresis (2DE) and transferring to a blotting membrane, major proteins are detected by a staining of direct blue 71 in a neutral solution. The carboxylesterase on the membrane hydrolyzes phosphatidylcholine after the spot of carboxylesterase is e...

journal_title：Biotechnology and bioengineering

authors： Shimazaki Y,Miyamoto M

更新日期：2007-11-01 00:00:00

abstract：:Aggregates of Escherichia coli confined within hollowfiber reactors were either formed in place by culturing cells within the reactors, or were prepared by injecting a cell suspension that had been concentrated by centrifugation. The effective diffusive permeability of an uncharged nonreacting tracer, nitrous oxide, w...

journal_title：Biotechnology and bioengineering

authors： Libicki SB,Salmon PM,Robertson CR

更新日期：1988-06-20 00:00:00

abstract：:The productivity of Escherichia coli as a producer of recombinant proteins is affected by its metabolic properties, especially by acetate production. Two commercially used E. coli strains, BL21 (lambdaDE3) and JM109, differ significantly in their acetate production during batch fermentation at high initial glucose con...

journal_title：Biotechnology and bioengineering

authors： van de Walle M,Shiloach J

更新日期：1998-01-05 00:00:00

abstract：:A novel acetone-butanol production process was developed which integrates a repeated fed-batch fermentation with continuous product removal and cell recycle. The inhibitory product concentrations of the fermentation by Clostridium acetobutylicum were reduced by the simultaneous extraction process using polyvinylpyridi...

journal_title：Biotechnology and bioengineering

authors： Yang X,Tsao GT

更新日期：1995-08-20 00:00:00

abstract：:Frozen bagged seed inoculum was prepared, thawed and tested for seven cultures. Thawing techniques were developed and other key influences on thawing rate were quantified; seed bag thawing without a water bath rarely required more than 4 to 5 h and was as short as 0.5 to 1 h for lower fill volume bags. Testing include...

journal_title：Biotechnology and bioengineering

authors： Junker B,Seeley A,Lester M,Kovatch M,Schmitt J,Boryscwicz S,Lynch J,Zhang J,Greasham R

更新日期：2002-09-20 00:00:00

abstract：:The aim of this study was to determine the feasibility of using high pressure CO2 for sterilization of Ginseng powder, as an alternative method to conventional techniques such as gamma-irradiation and ethylene oxide. The Ginseng sample used in this study was originally contaminated with fungi and 5 x 10(7) bacteria/g ...

journal_title：Biotechnology and bioengineering

authors： Dehghani F,Annabi N,Titus M,Valtchev P,Tumilar A

更新日期：2009-02-01 00:00:00

abstract：:While feeding protocols and ectopic expression of anti-apoptotic genes have been used to improve the viability of hybridoma cell lines, the effect of the expression levels of survival genes on the behavior of hybridomas following nutrient supplementation is unknown. In this study, we compared the behavior of the Sp2/0...

journal_title：Biotechnology and bioengineering

authors： Charbonneau JR,Furtak T,Lefebvre J,Gauthier ER

更新日期：2003-02-05 00:00:00

abstract：:Studies have been conducted in a gas circulation type fermentor in order to characterize the ethanol fermentation of uncooked cassava starch with Rhizopus koji. Results showed that ethanol concentration reached 13-14% (v/v) in 4-day broth, and the maximum productivity of ethanol was 2.3 g ethanol/L broth h. This produ...

journal_title：Biotechnology and bioengineering

authors： Fujio Y,Ogata M,Ueda S

更新日期：1985-08-01 00:00:00

abstract：:The possibility of biologically detoxifying a contaminated soil from an Italian dump site containing about 1500 mg/kg (in dry soil) of polychlorinated biphenyls was studied in the laboratory in this work. The soil, which contained indigenous aerobic bacteria capable of growing on biphenyl or on monochlorobenzoic acids...

journal_title：Biotechnology and bioengineering

authors： Fava F,Bertin L

更新日期：1999-07-20 00:00:00

abstract：:A novel chemistry has been developed for the production of capsules composed of a hydrophobic liquid core surrounded by a cross-linked polyacrylamide/alginate membrane. These liquid-core capsules may be used in capsular perstraction for the removal of inhibitory products from bioprocesses and bioconversions. They have...

journal_title：Biotechnology and bioengineering

authors： Wyss A,von Stockar U,Marison IW

更新日期：2004-06-05 00:00:00

abstract：:Dihydrofolate reductase (DHFR) system is used to amplify the product gene to multiple copies in Chinese Hamster Ovary (CHO) cells for generating cell lines which produce the recombinant protein at high levels. The physiological changes accompanying the transformation of the non-protein secreting host cells to a high p...

journal_title：Biotechnology and bioengineering

authors： Vishwanathan N,Le H,Jacob NM,Tsao YS,Ng SW,Loo B,Liu Z,Kantardjieff A,Hu WS

更新日期：2014-03-01 00:00:00