Enhanced acetone-butanol fermentation using repeated fed-batch operation coupled with cell recycle by membrane and simultaneous removal of inhibitory products by adsorption.

abstract：:Lipase from Pseudomonas fluorescens biotype I was immobilized by adsorption of anion exchange resin using glutaraldehyde to enhance the adsorption. The activity yield of the immobilized lipase was very low (below 1%) when lipase activity was measured using emulsion substrate. The activity yield was 10-70% when lipase ...

journal_title：Biotechnology and bioengineering

authors： Kosugi Y,Tanaka H,Tomizuka N

更新日期：1990-09-01 00:00:00

abstract：:A highly active xylose specific transporter without glucose inhibition is highly desirable in cost-effective production of biofuels from lignocellulosic biomass. However, currently available xylose specific transporters suffer from low overall activity and most are inhibited by glucose. In this study, we applied a dir...

journal_title：Biotechnology and bioengineering

authors： Wang M,Yu C,Zhao H

更新日期：2016-03-01 00:00:00

abstract：:The simultaneous growth and product formation in a microbial culture is an important feature of several laboratory, industrial, and environmental bioprocesses. Metabolic burden associated with product formation in these bioprocesses may lead to growth advantage of a nonproducing mutant leading to a loss of the produci...

journal_title：Biotechnology and bioengineering

authors： Varma A,Palsson BO

更新日期：1994-02-20 00:00:00

abstract：:Targeted gene integration into site-specific loci can be achieved in Chinese hamster ovary (CHO) cells via CRISPR/Cas9 genome editing technology and the homology-directed repair (HDR) pathway. The low efficiency of HDR often requires antibiotic selection, which limits targeted integration of multiple genes at multiple...

journal_title：Biotechnology and bioengineering

authors： Lee JS,Grav LM,Pedersen LE,Lee GM,Kildegaard HF

更新日期：2016-11-01 00:00:00

abstract：:Recent studies have demonstrated the feasibility of using ultrafiltration for the purification of plasmid DNA, but there is still little understanding of the factors governing DNA transmission. Experimental data were obtained for the transmission of a 3.0 kbp supercoiled plasmid DNA through composite regenerated cellu...

journal_title：Biotechnology and bioengineering

authors： Latulippe DR,Zydney AL

更新日期：2008-02-01 00:00:00

abstract：:In enzymatic lysis of yeast for the recovery of intracellular proteins, the rupture of whole cells is caused by the action of a lytic system consisting primarily of protease and glucanase. A first-principles mechanism for the lytic reaction based on a two-layer model of the wall structure and a burst model for the dis...

journal_title：Biotechnology and bioengineering

authors： Liu LC,Prokopakis GJ,Asenjo JA

更新日期：1988-10-20 00:00:00

abstract：:Beta defensins are antimicrobial peptides (AMPs) with a broad spectrum antimicrobial behavior against pathogens while having minimal tendency to incur pathogen resistance. Human β-defensin 28 (hBD28) is a strongly cationic AMP and hence hypothesized to be highly effective in permeabilizing negatively-charged pathogen ...

journal_title：Biotechnology and bioengineering

authors： Tay DK,Rajagopalan G,Li X,Chen Y,Lua LH,Leong SS

更新日期：2011-03-01 00:00:00

abstract：:Glycosylation is a key critical quality attribute for monoclonal antibodies and other recombinant proteins because of its impact on effector mechanisms and half-life. In this study, a variety of compounds were evaluated for their ability to modulate glycosylation profiles of recombinant monoclonal antibodies produced ...

journal_title：Biotechnology and bioengineering

authors： Ehret J,Zimmermann M,Eichhorn T,Zimmer A

更新日期：2019-04-01 00:00:00

abstract：:An option for fertility preservation for women facing a cancer diagnosis involves the cryopreservation of ovarian tissue for later re-transplantation or in vitro culture, with in vitro culture preferred to avoid reintroduction of the cancer. Small, immature follicles survive the freeze-thaw process, and can be matured...

journal_title：Biotechnology and bioengineering

authors： Xu M,Banc A,Woodruff TK,Shea LD

更新日期：2009-06-01 00:00:00

abstract：:Optically pure compounds are important in the synthesis of fine chemicals. Using directed evolution of enzymes to obtain biocatalysts that can selectively produce high-value chiral chemicals is often time-, money-, and resource-intensive; traditional semi-rational designs based on structural data and docking experimen...

journal_title：Biotechnology and bioengineering

authors： Cheng Z,Peplowski L,Cui W,Xia Y,Liu Z,Zhang J,Kobayashi M,Zhou Z

更新日期：2018-03-01 00:00:00

abstract：:The investigation of new adjuvants is essential for the development of efficacious vaccines. Chitosan (CS), a derivative of chitin, has been shown to act as an adjuvant, improving vaccine-induced immune responses. However, the effect of CS molecular weight (MW) on this adjuvanticity has not been investigated, despite ...

journal_title：Biotechnology and bioengineering

authors： Lampe AT,Farris EJ,Brown DM,Pannier AK

更新日期：2020-12-07 00:00:00

abstract：:Efficient cofactor regeneration and reuse are highly desired for many important biotransformation applications. Here we show for the first time that cofactor NAD(H) covalently attached to micro particles, which can be easily recovered and reused, effectively mediated multistep reactions catalyzed by enzymes that were ...

journal_title：Biotechnology and bioengineering

authors： El-Zahab B,Donnelly D,Wang P

更新日期：2008-02-15 00:00:00

abstract：:The production of Bacillus thuringiensis spores was investigated in a bioreactor incorporating a ceramic membrane filter to improve spore concentration and volumetric productivity. Two cultivation methods were used in this study: a total cell retention culture (TCRC), and a two-stage continuous culture with partial ce...

journal_title：Biotechnology and bioengineering

authors： Kang BC,Lee SY,Chang HN

更新日期：1993-11-05 00:00:00

abstract：:The methanotrophic bacterium Methylococcus capsulatus is capable of assimilating methane and oxygen into protein-rich biomass, however, the diverse metabolism of the microorganism also allows for several undesired cometabolic side-reactions to occur. In this study, the ammonia cometabolism in Methylococcus capsulatus ...

journal_title：Biotechnology and bioengineering

authors： Petersen LAH,Lieven C,Nandy SK,Villadsen J,Jørgensen SB,Christensen I,Gernaey KV

更新日期：2019-11-01 00:00:00

abstract：:While feeding protocols and ectopic expression of anti-apoptotic genes have been used to improve the viability of hybridoma cell lines, the effect of the expression levels of survival genes on the behavior of hybridomas following nutrient supplementation is unknown. In this study, we compared the behavior of the Sp2/0...

journal_title：Biotechnology and bioengineering

authors： Charbonneau JR,Furtak T,Lefebvre J,Gauthier ER

更新日期：2003-02-05 00:00:00

abstract：:The activities of lipase from Candida cylindracea and Rhizopus delemar have been investigated in water/AOT/iso-octane reverse micellar media through the use of two esterification reactions: fatty acid-alcohol esterification and glyceride synthesis. Such media promotes the occurrence of these two lipase-catalyzed react...

journal_title：Biotechnology and bioengineering

authors： Hayes DG,Gulari E

更新日期：1990-04-05 00:00:00

abstract：:High-level expression of recombinant penicillin acylase (PAC) using the strong trc promoter system in Escherichia coli is frequently limited by the processing and folding of PAC precursors (proPAC) in the periplasm, resulting in physiological stress and inclusion body formation in this compartment. Periplasmic heat-sh...

journal_title：Biotechnology and bioengineering

authors： Wu MS,Pan KL,Chou CP

更新日期：2007-04-01 00:00:00

abstract：:The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high-value antioxidant astaxanthin for over two decades, due to its superior ability to synthesize astaxanthin under adverse culture conditions. However, slow vegetative growth under favorable culture conditions and ...

journal_title：Biotechnology and bioengineering

authors： Zhang Z,Wang B,Hu Q,Sommerfeld M,Li Y,Han D

更新日期：2016-10-01 00:00:00

abstract：:Fast and efficient production of recombinant proteins for structural and functional studies is a crucial issue for research and for industry. To this end, we have developed an efficient system to generate in less than 2 months, starting from the cDNA, pools of CHO cells stably expressing high-level of recombinant prot...

journal_title：Biotechnology and bioengineering

authors： Gaillet B,Gilbert R,Broussau S,Pilotte A,Malenfant F,Mullick A,Garnier A,Massie B

更新日期：2010-06-01 00:00:00

abstract：:To alleviate plasmid instability and to prolong the production phase of subtilisin, integrable plasmid and spore mutants are used. Compared with batch-type shake flask cultures, spore mutants' ability to produce subtilisin can be well pronounced in fed-batch and continuous cultures. Hence, the two culture methods make...

journal_title：Biotechnology and bioengineering

authors： Oh MK,Kim BG,Park SH

更新日期：1995-09-20 00:00:00

abstract：:Genome-scale flux analysis of Escherichia coli DH5alpha growth in a complex medium was performed to investigate the relationship between the uptake of various nutrients and their metabolic outcomes. During the exponential growth phase, we observed a sequential consumption order of serine, aspartate and glutamate in th...

journal_title：Biotechnology and bioengineering

authors： Selvarasu S,Ow DS,Lee SY,Lee MM,Oh SK,Karimi IA,Lee DY

更新日期：2009-02-15 00:00:00

abstract：:Modern large-scale agricultural practices that incorporate high density farming with subtherapeutic antibiotic dosing are considered a major contributor to the rise of antibiotic-resistant bacterial infections of humans with species of Salmonella being a leading agriculture-based bacterial infection. Microcin J25, a p...

journal_title：Biotechnology and bioengineering

authors： Ritter SC,Yang ML,Kaznessis YN,Hackel BJ

更新日期：2018-10-01 00:00:00

abstract：:A two-dimensional model for quantitative evaluation of the effect of convective and diffusive substrate transport on biofilm heterogeneity was developed. The model includes flow computation around the irregular biofilm surface, substrate mass transfer by convection and diffusion, biomass growth, and biomass spreading....

journal_title：Biotechnology and bioengineering

authors： Picioreanu C,Van Loosdrecht MC,Heijnen JJ

更新日期：2000-09-05 00:00:00

abstract：:Recently it has been established that low molecular weight displacers can be successfully employed for the purification of proteins in hydrophobic interaction chromatography (HIC) systems. This work investigates the utility of this technique for the purification of an industrial protein mixture. The study involved the...

journal_title：Biotechnology and bioengineering

authors： Sunasara KM,Xia F,Gronke RS,Cramer SM

更新日期：2003-05-05 00:00:00

abstract：:Glycerol is considered as an ideal feedstock for producing bioplastics via bacterial fermentation due to its ubiquity, low price, and high degree of reduction substrate. In this work, we study the yield and cause of limitation in poly(3-hydroxybutyrate) (PHB) production from glycerol. Compared to glucose-based PHB pro...

journal_title：Biotechnology and bioengineering

authors： Tanadchangsaeng N,Yu J

更新日期：2012-11-01 00:00:00

abstract：:Batch hydrolysis kinetics of paper birch (Betula papyrifera) xylan and its associated acetyl groups in dilute sulfuric acid have been measured for acid concentrations of between 0.04 and 0.18M and temperatures of between 100 and 170 degrees C. Only 5% of the cellulose was hydrolyzed for up to 85% xylan removal. Rate d...

journal_title：Biotechnology and bioengineering

authors： Maloney MT,Chapman TW,Baker AJ

更新日期：1985-03-01 00:00:00

abstract：:Overexpression of recombinant proteins in Escherichia coli often leads to a severe growth retardation of the host cells. The phage T7 promoter phi10 in a pET vector was utilized to express human superoxide dismutase. Induction with IPTG lead to an increase in protein content and cell size and a termination of cell div...

journal_title：Biotechnology and bioengineering

authors： Soriano E,Borth N,Katinger H,Mattanovich D

更新日期：2002-10-05 00:00:00

abstract：:The combination of Sephadex G-15 and ion-exchange resin columns allows one-step desalting and separation of cellodextrins using water as the sole eluent. The column apparatus described in this paper has the potential of producing up to 3 g cellodextrins in one day. In addition, the columns described are stable and do ...

journal_title：Biotechnology and bioengineering

authors： Markovits A

更新日期：1978-10-01 00:00:00

abstract：:While elastin-like polypeptides and peptides (ELPs) have been used for various stimulus-responsive applications, details of their switching remain unclear. We therefore constructed a novel series of filamentous phage particles displaying a high surface density of short ELPs. The surface display of ELPs did not disrupt...

journal_title：Biotechnology and bioengineering

authors： Hathorne AP,Bermudez H

更新日期：2013-07-01 00:00:00

abstract：:To build complex genetic networks with predictable behaviors, synthetic biologists use libraries of modular parts that can be characterized in isolation and assembled together to create programmable higher-order functions. Characterization experiments and computational models for gene regulatory parts operating in iso...

journal_title：Biotechnology and bioengineering

authors： Westbrook A,Tang X,Marshall R,Maxwell CS,Chappell J,Agrawal DK,Dunlop MJ,Noireaux V,Beisel CL,Lucks J,Franco E

更新日期：2019-05-01 00:00:00