High- and low-molecular-weight chitosan act as adjuvants during single-dose influenza A virus protein vaccination through distinct mechanisms.


:The investigation of new adjuvants is essential for the development of efficacious vaccines. Chitosan (CS), a derivative of chitin, has been shown to act as an adjuvant, improving vaccine-induced immune responses. However, the effect of CS molecular weight (MW) on this adjuvanticity has not been investigated, despite MW having been shown to impact CS biological properties. Here, two MW variants of CS were investigated for their ability to enhance vaccine-elicited immune responses in vitro and in vivo, using a single-dose influenza A virus (IAV) protein vaccine model. Both low-molecular-weight (LMW) and high-molecular-weight (HMW) CS-induced interferon regulatory factor pathway signaling, antigen-presenting cell activation, and cytokine messenger RNA (mRNA) production, with LMW inducing higher mRNA levels at 24 h and HMW elevating mRNA responses at 48 h. LMW and HMW CS also induced adaptive immune responses after vaccination, indicated by enhanced immunoglobulin G production in mice receiving LMW CS and increased CD4 interleukin 4 (IL-4) and IL-2 production in mice receiving HMW CS. Importantly, both LMW and HMW CS adjuvantation reduced morbidity following homologous IAV challenge. Taken together, these results support that LMW and HMW CS can act as adjuvants, although this protection may be mediated through distinct mechanisms based on CS MW.


Biotechnol Bioeng


Lampe AT,Farris EJ,Brown DM,Pannier AK




Has Abstract


2020-12-07 00:00:00






  • Glutamate synthesis via photoreduction of NADP+ by photostable chlorophyllide coupled with polyethylene-glycol.

    abstract::Chlorophyllide a was coupled with alpha-(3-aminopropyl)-omega-methoxypoly(oxyethylene) (PEG-NH2) to form a PEG-chlorophyllide conjugate through an acid-amide bond. The conjugate catalyzed the reduction of methylviologen in the presence of 2-mercaptoethanol. It also catalyzed the photoreduction of NADP+ or NAD+ in the ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Asada H,Itoh T,Kodera Y,Matsushima A,Hiroto M,Nishimura H,Inada Y

    更新日期:2001-01-01 00:00:00

  • Application of hydrophobic interaction displacement chromatography for an industrial protein purification.

    abstract::Recently it has been established that low molecular weight displacers can be successfully employed for the purification of proteins in hydrophobic interaction chromatography (HIC) systems. This work investigates the utility of this technique for the purification of an industrial protein mixture. The study involved the...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Sunasara KM,Xia F,Gronke RS,Cramer SM

    更新日期:2003-05-05 00:00:00

  • Metabolite and isotopomer balancing in the analysis of metabolic cycles: I. Theory.

    abstract::Proper analysis of label distribution in metabolic pathway intermediates is critical for correct interpretation of experimental data and strategic experimental design. While, for example, 13C nuclear magnetic resonance (NMR) spectroscopy is usually limited to the measurement of degrees of 13C enrichment, more informat...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Klapa MI,Park SM,Sinskey AJ,Stephanopoulos G

    更新日期:1999-02-01 00:00:00

  • Co-culturing Chlorella minutissima with Escherichia coli can increase neutral lipid production and improve biodiesel quality.

    abstract::Lipid productivity and fatty acid composition are important metrics for the production of high quality biodiesel from algae. Our previous results showed that co-culturing the green alga Chlorella minutissima with Escherichia coli under high-substrate mixotrophic conditions enhanced both culture growth and crude lipid ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Higgins BT,Labavitch JM,VanderGheynst JS

    更新日期:2015-09-01 00:00:00

  • Experimental evaluation of a model for cometabolism: Prediction of simultaneous degradation of trichloroethylene and methane by a methanotrophic mixed culture.

    abstract::A model for cometabolism is verified experimentally for a defined methanotrophic mixed culture. The model includes the effects of cell growth, endogenous cell decay, product toxicity, and competitive inhibition with the assumption that cometabolic transformation rates are enhanced by reducing power obtained from oxida...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chang WK,Criddle CS

    更新日期:1997-12-05 00:00:00

  • Model-based derivation, analysis and control of unstable microaerobic steady-states--considering Rhodospirillum rubrum as an example.

    abstract::Microaerobic (oxygen-limited) conditions are critical for inducing many important microbial processes in industrial or environmental applications. At very low oxygen concentrations, however, the process performance often suffers from technical limitations. Available dissolved oxygen measurement techniques are not sens...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Carius L,Rumschinski P,Faulwasser T,Flockerzi D,Grammel H,Findeisen R

    更新日期:2014-04-01 00:00:00

  • Pretreatment of corn stover by combining ionic liquid dissolution with alkali extraction.

    abstract::Pretreatment plays an important role in the efficient enzymatic hydrolysis of biomass into fermentable sugars for biofuels. A highly effective pretreatment method is reported for corn stover which combines mild alkali-extraction followed by ionic liquid (IL) dissolution of the polysaccharides and regeneration (recover...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Geng X,Henderson WA

    更新日期:2012-01-01 00:00:00

  • Enhanced acetone-butanol fermentation using repeated fed-batch operation coupled with cell recycle by membrane and simultaneous removal of inhibitory products by adsorption.

    abstract::A novel acetone-butanol production process was developed which integrates a repeated fed-batch fermentation with continuous product removal and cell recycle. The inhibitory product concentrations of the fermentation by Clostridium acetobutylicum were reduced by the simultaneous extraction process using polyvinylpyridi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yang X,Tsao GT

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  • Annotation of additional evolutionary conserved microRNAs in CHO cells from updated genomic data.

    abstract::MicroRNAs are small non-coding RNAs that play a critical role in post-transcriptional control of gene expression. Recent publications of genomic sequencing data from the Chinese Hamster (CGR) and Chinese hamster ovary (CHO) cells provide new tools for the discovery of novel miRNAs in this important production system. ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Diendorfer AB,Hackl M,Klanert G,Jadhav V,Reithofer M,Stiefel F,Hesse F,Grillari J,Borth N

    更新日期:2015-07-01 00:00:00

  • Kinetics of organic compound removal from waste gases with a biological filter.

    abstract::In order to eliminate organic pollutants in waste gases, a biological filter bed technique has been developed, with a high self-regenerating capacity and a low pressure drop. The bed consists of an appropriate filling material (mainly peat compost), in order to let the microorganisms grow on the solid surface and to s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


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  • Evaluation of selectivity changes in HIC systems using a preferential interaction based analysis.

    abstract::It is well established that salt enhances the interaction between solutes (e.g., proteins, displacers) and the weak hydrophobic ligands in hydrophobic interaction chromatography (HIC) and that various salts (e.g., kosmotropes, chaotropes, and neutral) have different effects on protein retention. In this article, the s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Xia F,Nagrath D,Garde S,Cramer SM

    更新日期:2004-08-05 00:00:00

  • The process utility of thermotolerant methylotrophic bacteria: II. An evaluation of transient responses.

    abstract::In Part II, the process utility of a thermotolerant methylotrophic bacterium is evaluated with respect to its dynamic response when various substrate or nutrient pulses are imposed on it while growing under steady-state conditions in a chemostat. The pulses investigated were methanol pulses on methanol-, methanol/form...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Al-Awadhi N,Egli T,Hamer G,Mason CA

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  • Photo inactivation of virus particles in microfluidic capillary systems.

    abstract::It has long been established that UVC light is a very effective method for inactivating pathogens in a fluid, yet the application of UVC irradiation to modern biotechnological processes is limited by the intrinsic short penetration distance of UVC light in optically dense protein solutions. This experimental and numer...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ren Y,Crump CM,Mackley MM,Li Puma G,Reis NM

    更新日期:2016-07-01 00:00:00

  • Novel non-viral vectors for gene delivery: synthesis of a second-generation library of mono-functionalized poly-(guanidinium)amines and their introduction into cationic lipids.

    abstract::The development of new gene delivery technologies is a prerequisite towards gene therapy clinical trials. Because gene delivery mediated by viral vectors remains of limited scope due to immunological and propagation risks, the development of new non-viral gene delivery systems is of crucial importance. We have synthes...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Byk G,Soto J,Mattler C,Frederic M,Scherman D

    更新日期:1998-04-01 00:00:00

  • Quantitative determination of cellulase concentration as distinct from cell concentration in studies of microbial cellulose utilization: analytical framework and methodological approach.

    abstract::In analyzing microbial cellulose utilization, it would be useful to independently measure the mass concentration of cells and cellulase enzymes. Such measurements would allow investigation of the allocation of cellular resources between synthesis of cells and cellulase, in vivo cell- and cellulase-specific cellulose h...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Lynd LR,Zhang Y

    更新日期:2002-02-15 00:00:00

  • Alginate microparticles as novel carrier for oral insulin delivery.

    abstract::Alginate microparticles produced by emulsification/internal gelation were investigated as a promising carrier for insulin delivery. The procedure involves the dispersion of alginate solution containing insulin protein, into a water immiscible phase. Gelation is triggered in situ by instantaneous release of ionic calci...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Reis CP,Ribeiro AJ,Neufeld RJ,Veiga F

    更新日期:2007-04-01 00:00:00

  • Alpha-chymotrypsin catalysis in imidazolium-based ionic liquids.

    abstract::The transesterification reaction of N-acetyl-L-phenylalanine ethyl ester with 1-propanol catalyzed by alpha-chymotrypsin was examined in the ionic liquids 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim][PF(6)]) and 1-octyl-3-methylimidazolium hexafluorophosphate ([omim][PF(6)]), and in combination with supercr...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Laszlo JA,Compton DL

    更新日期:2001-10-20 00:00:00

  • Plasmid DNA production for pharmaceutical use: role of specific growth rate and impact on process design.

    abstract::Application of plasmid DNA as pharmaceutical to be used in gene therapy and vaccination has been investigated intensively in recent years. To be able to provide sufficient material that is in accordance with quality of pharmaceutical grade it is mandatory to gain comprehensive process knowledge which is even requested...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Bohle K,Ross A

    更新日期:2011-09-01 00:00:00

  • Reaction characteristics and stability of a membrane-bound enzyme reconstituted in bilayers of liposomes.

    abstract::The effects ensuing from the interaction between membrane-bound sarcosine dehydrogenase and the surrounding lipids as well as the effects of membrane fluidity were described in this study. A 25-fold activation was observed upon the reconstitution of the enzyme in bilayers of SUVs made of DMPC. The considerable decreas...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kheirolomoom A,Katoh S,Sada E,Yoshida K

    更新日期:1991-04-15 00:00:00

  • Tuning biphenyl dioxygenase for extended substrate specificity.

    abstract::Highly substituted polychlorinated biphenyls (PCBs) are known to be very resistant to aerobic biodegradation, particularly the initial attack by biphenyl dioxygenase. Functional evolution of the substrate specificity of biphenyl dioxygenase was demonstrated by DNA shuffling and staggered extension process (StEP) of th...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Brühlmann F,Chen W

    更新日期:1999-06-05 00:00:00

  • Metabolic flux modeling of detoxification of acetic acid by Ralstonia eutropha at slightly alkaline pH levels.

    abstract::Ralstonia eutropha grows on and produces polyhydroxyalkanoates (PHAs) from fermentation acids. Acetic acid, one major organic acid from acidogenesis of organic wastes, has an inhibitory effect on the bacterium at slightly alkaline pH (6 g HAc/L at pH 8). The tolerance of R. eutropha to acetate, however, was increased ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yu J,Wang J

    更新日期:2001-06-20 00:00:00

  • A gaussian model for substrates of entangled cross-linked poly(ethylene glycol) in biomedical applications.

    abstract::Cells usually spread on a synthetic substrate through bonds between receptors and chemical groups on the substrate (ligands). Therefore, it is valuable to study the effects of the average number density of these chemical groups and the average distance between them to model and predict the cell behavior. Poly(ethylene...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Eskandari M,Brey E,Cinar A

    更新日期:2011-02-01 00:00:00

  • Impact of external calcium and calcium sensors on ginsenoside Rb1 biosynthesis by Panax notoginseng cells.

    abstract::The effects of external calcium concentrations on biosynthesis of ginsenoside Rb1 and several calcium signal sensors were quantitatively investigated in suspension cultures of Panax notoginseng cells. It was observed that the synthesis of intracellular ginsenoside Rb1 in 3-day incubation was dependent on the medium Ca...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yue CJ,Zhong JJ

    更新日期:2005-02-20 00:00:00

  • Tuning of the enzyme ratio in a neutral redox convergent cascade: A key approach for an efficient one-pot/two-step biocatalytic whole-cell system.

    abstract::The efficiency of a versatile in vivo cascade involving a promiscuous alcohol dehydrogenase, obtained from a biodiversity search, and a Baeyer-Villiger monooxygenase was enhanced by the independent control of the production level of each enzyme to produce ε-caprolactone and 3,4-dihydrocoumarin. This goal was achieved ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ménil S,Petit JL,Courvoisier-Dezord E,Debard A,Pellouin V,Reignier T,Sergent M,Deyris V,Duquesne K,de Berardinis V,Alphand V

    更新日期:2019-11-01 00:00:00

  • Application of continuous zone electrophoresis to preparative separation of proteins.

    abstract::A comprehensive study of the application of continuous zone electrophoresis to preparative separation of proteins in free solution is presented. First, the influence of electric field strength, buffer residence time in the chamber, sample flow rate, and sample concentration on separation resolution and throughput were...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Nath S,Schütte H,Hustedt H,Deckwer WD

    更新日期:1993-09-20 00:00:00

  • Kinetic model of biosurfactant-enhanced hexadecane biodegradation by Pseudomonas aeruginosa.

    abstract::Many sites of environmental concern contain groundwater contaminated with nonaqueous phase liquids (NAPL). In such sites interfacial processes may affect both the equilibrium and kinetic behavior of the system. In particular, insoluble hydrocarbon partitioning and microbial biodegradation of insoluble hydrocarbon are ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Sekelsky AM,Shreve GS

    更新日期:1999-05-20 00:00:00

  • Main and interaction effects of acetic acid, furfural, and p-hydroxybenzoic acid on growth and ethanol productivity of yeasts.

    abstract::The influence of the factors acetic acid, furfural, and p-hydroxybenzoic acid on the ethanol yield (YEtOH) of Saccharomyces cerevisiae, bakers' yeast, S. cerevisiae ATCC 96581, and Candida shehatae NJ 23 was investigated using a 2(3)-full factorial design with 3 centrepoints. The results indicated that acetic acid inh...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Palmqvist E,Grage H,Meinander NQ,Hahn-Hägerdal B

    更新日期:1999-04-05 00:00:00

  • Tobacco smoke as inducer for gas phase-controlled transgene expression in mammalian cells and mice.

    abstract::Capitalizing on components evolved to metabolize ethanol in Aspergillus nidulans, we previously designed the first molecular gas-gene expression interface using gaseous acetaldehyde as the major inducer. This fungus-derived acetaldehyde-inducible gene regulation (AIR) system operated perfectly and enabled precise and ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Weber W,Spielmann M,Daoud El-Baba M,Keller B,Aubel D,Fussenegger M

    更新日期:2005-06-30 00:00:00

  • Conversion of L-sorbose to L-sorbosone by immobilized cells of Gluconobacter melanogenus IFO 3293.

    abstract::Gluconobacter melanogenus IFO 3293 cells capable of converting L-sorbose to L-sorbosone were immobilized in polyacrylamide gel. The preferred polymer composition for high activity and stability was determined to contain a total monomer concentration of 7.2% and 16.6% crosslinking agent. No significant differences in o...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Martin CK,Perlman D

    更新日期:1976-02-01 00:00:00

  • Application of an electric DNA-chip for the expression analysis of bioprocess-relevant marker genes of Bacillus subtilis.

    abstract::The knowledge of critical process-relevant genes can be used for an improved control of bioprocesses. So far bioprocess-relevant marker genes can be analyzed by established expression analysis methods only off-line. In this study, an alternative approach for a potential at-line monitoring of gene expression during bio...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Jürgen B,Barken KB,Tobisch S,Pioch D,Wümpelmann M,Hecker M,Schweder T

    更新日期:2005-11-05 00:00:00