High- and low-molecular-weight chitosan act as adjuvants during single-dose influenza A virus protein vaccination through distinct mechanisms.

abstract：:The main processes involved in enhanced biological phosphorus removal (EBPR) under anaerobic and subsequently aerobic conditions are widely described in the literature. Polyphosphate accumulating organisms (PAO) are the organisms responsible for this process. However, the mechanisms of PAO are not fully established ye...

journal_title：Biotechnology and bioengineering

authors： Guisasola A,Pijuan M,Baeza JA,Carrera J,Casas C,Lafuente J

更新日期：2004-03-30 00:00:00

abstract：:Yeast alcohol dehydrogenase (ADH) solutions (approximately 1 mg/ml, pH 7) were sheared in a coaxial cylindrical viscometer. This was fitted with a lid sealing the contents from the atmosphere and preventing evaporation. At 30 degrees C after a total of 5 hr intermittent shearing at 683 sec-1 no losses of activity were...

journal_title：Biotechnology and bioengineering

authors： Thomas CR,Nienow AW,Dunnill P

更新日期：1979-12-01 00:00:00

abstract：:An in vitro toxicity testing system that combines perfusion of a membrane-supported tissue model with measurement of trans-epithelial electrical resistance (TER) is described. The system is fully automated and allows for continuous TER measurements during exposure to a test agent. The utility of this system is demonst...

journal_title：Biotechnology and bioengineering

authors： Pasternak AS,Miller WM

更新日期：1996-06-05 00:00:00

abstract：:As cartilage is incapable of self-healing upon severe degeneration because of the lack of blood vessels, cartilage tissue engineering is gaining importance in the treatment of cartilage defects. This study was designed to improve cartilage tissue regeneration by expressing tissue transglutaminase variant 2 (TGM2_v2) i...

journal_title：Biotechnology and bioengineering

authors： Calikoglu Koyuncu AC,Nayman AH,Telci D,Torun Kose G

更新日期：2020-06-01 00:00:00

abstract：:The clustered regularly interspaced short palindromic repeats (CRISPR) system-associated Cas9 endonuclease is a molecular tool that enables specific sequence editing with high efficiency. In this study, we have explored the use of CRISPR/Cas9 system for the engineering of baculovirus. We have shown that the delivering...

journal_title：Biotechnology and bioengineering

authors： Pazmiño-Ibarra V,Mengual-Martí A,Targovnik AM,Herrero S

更新日期：2019-11-01 00:00:00

abstract：:The thermophilic fungus Sporotrichum cellulophilum was immobilized with nonwoven materials for cellulase production. The cellulose powder concentration in the medium was an important factor controlling cellulase production. When the cellulose powder concentration in the nonwoven materials was more than 4%, cellulase p...

journal_title：Biotechnology and bioengineering

authors： Tamada M,Kasai N,Kumakura M,Kaetsu I

更新日期：1986-08-01 00:00:00

abstract：:The relationship between local mass transfer coefficient and fluid velocity in heterogenous biofilms was investigated by combining microelectrodes and confocal scanning laser microscopy (CSLM). The biofilms were grown for up to 7 days and consisted of cell clusters separated by interstitial channels. Mass transfer coe...

journal_title：Biotechnology and bioengineering

authors： Stoodley P,Yang S,Lappin-Scott H,Lewandowski Z

更新日期：1997-12-20 00:00:00

abstract：:Lipid productivity and fatty acid composition are important metrics for the production of high quality biodiesel from algae. Our previous results showed that co-culturing the green alga Chlorella minutissima with Escherichia coli under high-substrate mixotrophic conditions enhanced both culture growth and crude lipid ...

journal_title：Biotechnology and bioengineering

authors： Higgins BT,Labavitch JM,VanderGheynst JS

更新日期：2015-09-01 00:00:00

abstract：:Although widely used in experimental and industrial situations, genetically engineered plasmids containing the lac promoter from Escherichia coli are subject to catabolite repression when grown in glucose-containing media. Several methods of overcoming this problem have been investigated by studying the expression of ...

journal_title：Biotechnology and bioengineering

authors： Warnes A,Stephenson JR,Fooks AR,Melling J,Brown MR

更新日期：1991-11-01 00:00:00

abstract：:In regenerative medicine, computer models describing bioreactor processes can assist in designing optimal process conditions leading to robust and economically viable products. In this study, we started from a (3D) mechanistic model describing the growth of neotissue, comprised of cells, and extracellular matrix, in a...

journal_title：Biotechnology and bioengineering

authors： Mehrian M,Guyot Y,Papantoniou I,Olofsson S,Sonnaert M,Misener R,Geris L

更新日期：2018-03-01 00:00:00

abstract：:Mixed culture of microorganisms immobilized onto Celite diatomaceous earth particles were used to degrade 3,4-dichloroaniline (34DCA) in a three-phase draft tube fluidized bed bioreactor. Biodegradation was confirmed as the dominant removal mechanism by measurements of the concomitant chloride ion evolution. Degradati...

journal_title：Biotechnology and bioengineering

authors： Livingston AG

更新日期：1991-07-01 00:00:00

abstract：:Reactor systems based on tapered fluidized beds are being developed for aqueous bioprocesses in which adhering microorganisms or immobilized active biological fractions are used. The use of a fluidized bed prevents biomass buildup, accommodates particulates in the feed stream, is compatible with gas sparging, and allo...

journal_title：Biotechnology and bioengineering

authors： Scott CD,Hancher CW

更新日期：1976-10-01 00:00:00

abstract：:A gene for ethionine resistance isolated from the yeast Saccharomyces cerevisiae DKD-5D-H conferred on the yeast cells resistance to seleno-L-methionine and capability to produce S-adenosyl-L-methionine in the cells. An enzymatic study of the L-methionine synthetic pathway of L-methionine proto- and auxotrophs and in ...

journal_title：Biotechnology and bioengineering

authors： Shiomi N,Fukuda H,Fukuda Y,Murata K,Kimura A

更新日期：1990-05-01 00:00:00

abstract：:Enzymatic bioreactors with both high flow characteristics and mechanical stability based on macroporous poly(2-vinyl-4, 4-dimethylazlactone-co-acrylamide-co-ethylene dimethacrylate) monoliths have been prepared. Covalent immobilization of trypsin on these support is achieved in a single reaction step using the azlacto...

journal_title：Biotechnology and bioengineering

authors： Xie S,Svec F,Fréchet JM

更新日期：1999-01-05 00:00:00

abstract：:The in vitro generation of cell-based three dimensional (3D) nerve tissue is an attractive subject to improve graft survival and integration into host tissue for neural tissue regeneration or to model biological events in stem cell differentiation. Although 3D organotypic culture strategies are well established for 3D...

journal_title：Biotechnology and bioengineering

authors： Kim BC,Jun SM,Kim SY,Kwon YD,Choe SC,Kim EC,Lee JH,Kim J,Suh JF,Hwang YS

更新日期：2017-04-01 00:00:00

abstract：:In this paper an extensive batch experiment of endogenous process behavior in an aerobic biodegradation process is presented. From these experimental data, comprising measurements of MLVSS (mixed liquor volatile suspended solids) and respiration rate, in a first step the states and unknown parameters in a four-compart...

journal_title：Biotechnology and bioengineering

authors： Keesman KJ,Spanjers H

更新日期：2000-05-20 00:00:00

abstract：:The production of dry-grind corn ethanol results in the generation of intermediate products, thin and whole stillage, which require energy-intensive downstream processing for conversion into commercial animal feed products. Hydrothermal carbonization of thin and whole stillage coupled with anaerobic digestion was inve...

journal_title：Biotechnology and bioengineering

authors： Wood BM,Jader LR,Schendel FJ,Hahn NJ,Valentas KJ,McNamara PJ,Novak PM,Heilmann SM

更新日期：2013-10-01 00:00:00

abstract：:A new ultrafiltration technique based on a multimembrane stack has been developed to fractionate solutes closer in size than conventionally possible. The technique is illustrated here by obtaining a pure protein product from a binary protein mixture. By employing membranes in series without any gaskets or spacers in-b...

journal_title：Biotechnology and bioengineering

authors： Feins M,Sirkar KK

更新日期：2004-06-20 00:00:00

abstract：:This article reports a study of the performance of membrane-attached biofilms grown in a single tube extractive membrane bioreactor (STEMS) used for the treatment of a synthetic wastewater containing a toxic VOC (1,2-dichloroethane [DCE]). Mass balances show that complete mineralization of DCE was achieved, and that t...

journal_title：Biotechnology and bioengineering

authors： Dos Santos LM,Livingston AG

更新日期：1995-07-05 00:00:00

abstract：:The kinetics of the cellulase-catalyzed conversion of soluble cellulose into glucose have been studied over a range of substrate concentrations and temperatures, and at pH values ranging from 4.75 to 7.0. Lineweaver-Burk plots were linear and led to V = 6.2muM/s and K(m) = 13.1 mM at pH 5.8 and 25.0 degrees C. The pK ...

journal_title：Biotechnology and bioengineering

authors： Banerjee DK,Laidler KJ

更新日期：1983-10-01 00:00:00

abstract：:Aggregates of Escherichia coli confined within hollowfiber reactors were either formed in place by culturing cells within the reactors, or were prepared by injecting a cell suspension that had been concentrated by centrifugation. The effective diffusive permeability of an uncharged nonreacting tracer, nitrous oxide, w...

journal_title：Biotechnology and bioengineering

authors： Libicki SB,Salmon PM,Robertson CR

更新日期：1988-06-20 00:00:00

abstract：:The objective of this study was to characterize the dynamic adaptation of the oxidative capacity of Saccharomyces cerevisiae to an increase in the glucose supply rate and its implications for the control of a continuous culture designed to produce biomass without allowing glucose to be diverted into the reductive meta...

journal_title：Biotechnology and bioengineering

authors： Duboc P,Cascão-Pereira LG,von Stockar U

更新日期：1998-03-05 00:00:00

abstract：:Escherichia coli cells with formate hydrogenlyase activity that were immobilized in agar beads produced hydrogen from glucose at the approximate yield of 0.6 mole of hydrogen per mole. Succinate or thiosulphate added to glucose increased hydrogen production two-fold. Thiosulphate did not increase the rate of hydrogen ...

journal_title：Biotechnology and bioengineering

authors： Nandi R,Dey S,Sengupta S

更新日期：2001-11-20 00:00:00

abstract：:We report on a simple method to rapidly generate very large libraries of genes encoding mutant proteins without the use of DNA amplification, and the application of this methodology in the construction of synthetic immunoglobulin variable heavy (V(H)) and light (V(kappa)) libraries. Four high quality, chemically synth...

journal_title：Biotechnology and bioengineering

authors： Ge X,Mazor Y,Hunicke-Smith SP,Ellington AD,Georgiou G

更新日期：2010-06-15 00:00:00

abstract：:Melanoma is now the fifth most common type of cancer in North America. At present, there is no optimal treatment for this cancer. However, the lowering of the tyrosine level can inhibit the growth of melanoma. Unfortunately, this diet restriction cannot be humanly tolerated and causes vomiting, nausea, and severe body...

journal_title：Biotechnology and bioengineering

authors： Yu B,Chang TM

更新日期：2004-06-30 00:00:00

abstract：:We have studied the growth rate dependence of hepatitis B surface antigen (HBsAg) p24(s) monomer and lipoprotein particle synthesis produced in Saccharomyces cerevisiae using galactose-limited continuous culture. The hepatitis B virus S gene, which encodes the p24(s) monomer, is transcribed under the control of the GA...

journal_title：Biotechnology and bioengineering

authors： Fu J,VanDusen WJ,Kolodin DG,O'Keefe DO,Herber WK,George HA

更新日期：1996-03-05 00:00:00

abstract：:Many microorganisms are capable of sequestering and concentrating heavy metals from their aqueous environment. While much research has beep carried out on the uptake of single species of metal ions, little attention seems to have been given to the study of multimetal ion systems. A mathematical model has previously be...

journal_title：Biotechnology and bioengineering

authors： Ting YP,Prince IG,Lawson F

更新日期：1991-03-05 00:00:00

abstract：:Preparative-scale purification of plasmid DNA has been attempted by diverse methods, including precipitation with solvents, salts, and detergents and chromatography with ion-exchange, reversed-phase, and size-exclusion columns. Chromatographic methods such as hydrophobic interaction chromatography (HIC), reversed phas...

journal_title：Biotechnology and bioengineering

authors： Lander RJ,Winters MA,Meacle FJ,Buckland BC,Lee AL

更新日期：2002-09-30 00:00:00

abstract：:Conventional aerobic nitrification was adversely affected by single pulse inputs of six different classes of industrially relevant chemical toxins: an electrophilic solvent (1-chloro-2,4-dinitrobenzene, CDNB), a heavy metal (cadmium), a hydrophobic chemical (1-octanol), an uncoupling agent (2,4-dinitrophenol, DNP), al...

journal_title：Biotechnology and bioengineering

authors： Kelly RT 2nd,Henriques ID,Love NG

更新日期：2004-03-20 00:00:00

abstract：:Cobalt chromium (CoCr) alloy is widely used in orthopedic implants but its functional longevity is susceptible to inflammation related complications. Reduction of the development of chronic inflammation on the biomaterial surface would enhance direct bone-implant bonding and improve implant survival and long-term resu...

journal_title：Biotechnology and bioengineering

authors： Tan HC,Poh CK,Cai Y,Soe MT,Wang W

更新日期：2013-03-01 00:00:00