Covalent immobilization of redox enzyme on electrospun nonwoven poly(acrylonitrile-co-acrylic acid) nanofiber mesh filled with carbon nanotubes: a comprehensive study.


:In this work, novel conductive composite nanofiber mesh possessing reactive groups was electrospun from solutions containing poly(acrylonitrile-co-acrylic acid) (PANCAA) and multi-walled carbon nanotubes (MWCNTs) for redoxase immobilization, assuming that the incorporated MWCNTs could behave as electrons transferor during enzyme catalysis. The covalent immobilization of catalase from bovine liver on the neat PANCAA nanofiber mesh or the composite one was processed in the presence of EDC/NHS. Results indicated that both the amount and activity retention of bound catalase on the composite nanofiber mesh were higher than those on the neat PANCAA nanofiber mesh, and the activity increased up to 42%. Kinetic parameters, K(m) and V(max), for the catalases immobilized on the composite nanofiber mesh were lower and higher than those on the neat one, respectively. This enhanced activity might be ascribed to either promoted electron transfer through charge-transfer complexes and the pi system of carbon nanotubes or rendered biocompatibility by modified MWCNTs. Furthermore, the immobilized catalases revealed much more stability after MWCNTs were incorporated into the polymer nanofiber mesh. However, there was no significant difference in optimum pH value and temperature, thermal stability and operational stability between these two immobilized preparations, while the two ones appeared more advantageous than the free in these properties. The effect of MWCNTs incorporation on another redox enzyme, peroxidase, was also studied and it was found that the activity increased by 68% in comparison of composite one with neat preparation.


Biotechnol Bioeng


Wang ZG,Ke BB,Xu ZK




Has Abstract


2007-07-01 00:00:00












  • Two-stage fermentation with bacteriophage lambda as an expression vector in Escherachia coli.

    abstract::The potential of bacteriophage lambda as an expression vector for a large scale production of cloned-gene proteins was evaluated in batch and continuous bioreactors using a temperature-sensitive mutant in the cl gene, which allows a simple manipulation of temperature as a means to control the phage in the lysogenic or...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Park TH,Seo JH,Lim HC

    更新日期:1991-02-20 00:00:00

  • Laboratory-scale production of acetoin plus diacetyl by Enterobacter cloacae ATCC 27613.

    abstract::Conditions for the laboratory-scale production of acetoin plus diacetyl by Enterobacter cloacae ATCC 27613 were studied. Thirty-five g acetoin plus diacetyl/50 g sucrose were obtained when fermentation was carried out in 2.5 liter medium containing 12.5 g peptone and 12.5 g yeast extract, at pH 7.0, in a 5 liter conic...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Gupta KG,Yadav NK,Dhawan S

    更新日期:1978-12-01 00:00:00

  • Kinetic studies on hybridoma cells immobilized in fixed bed reactors.

    abstract::Cultures with immobilized hybridoma cells were performed in fixed bed systems. "Steady state" values for volume-specific substrate uptake and metabolite production rates were determined at various perfusion rates and superficial flow velocities of the medium within the carrier matrix. Data from fixed bed volumes betwe...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Pörtner R,Rössing S,Koop M,Lüdemann I

    更新日期:1997-08-05 00:00:00

  • High-level heterologous production of propionate in engineered Escherichia coli.

    abstract::A propanologenic (i.e., 1-propanol-producing) bacterium Escherichia coli strain was previously derived by activating the genomic sleeping beauty mutase (Sbm) operon. The activated Sbm pathway branches out of the tricarboxylic acid (TCA) cycle at the succinyl-CoA node to form propionyl-CoA and its derived metabolites o...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Miscevic D,Mao JY,Moo-Young M,Chou CP

    更新日期:2020-05-01 00:00:00

  • Simulation of penicillin production in fed-batch cultivations using a morphologically structured model.

    abstract::A mathematical model is formulated to describe trends in biomass and penicillin formation as well as substrate consumption for fed-batch cultivations. The biomass is structured into three morphological compartments, and glucose and corn steep liquor are considered as substrates for growth. Penicillin formation is assu...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Zangirolami TC,Johansen CL,Nielsen J,Jørgensen SB

    更新日期:1997-12-20 00:00:00

  • Yeast suspension filtration: flux enhancement using an upward gas/liquid slug flow-application to continuous alcoholic fermentation with cell recycle.

    abstract::This study deals with the use of an upward gas/liquid slug flow to reduce tubular mineral membrane fouling. The injection of air into the feedstream is designed to create hydrodynamic conditions that destabilize the cake layer over the membrane surface inside the filtration module complex. Experimental study was carri...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Mercier M,Maranges C,Fonade C,Lafforgue-Delorme C

    更新日期:1998-04-05 00:00:00

  • Wine vinegar production using a noncommercial 100-litre bubble column reactor equipped with a novel type of dynamic sparger

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Fregapane G,Rubio-Fernandez H,Nieto J,Salvador MD

    更新日期:1999-04-20 00:00:00

  • Gas sensing in microplates with optodes: influence of oxygen exchange between sample, air, and plate material.

    abstract::Microplates with integrated optical oxygen sensors are a new tool to study metabolic rates and enzyme activities. Precise measurements are possible only if oxygen exchange between the sample and the environment is known. In this study we quantify gas exchange in plastic microplates. Dissolved oxygen was detected using...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Arain S,Weiss S,Heinzle E,John GT,Krause C,Klimant I

    更新日期:2005-05-05 00:00:00

  • Batch, fed-batch, and microcarrier cultures with CHO cell lines in a pressure-cycle driven miniaturized bioreactor.

    abstract::Miniaturized bioreactors for suspension cultures of animal cells, such as Chinese Hamster Ovary (CHO) cells, could improve bioprocess development through the ability to cheaply explore a wide range of bioprocess operating conditions. A miniaturized pressure-cycled bioreactor for animal cell cultures, described previou...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kim BJ,Zhao T,Young L,Zhou P,Shuler ML

    更新日期:2012-01-01 00:00:00

  • Thermo-responsive non-woven scaffolds for "smart" 3D cell culture.

    abstract::The thermo-responsive polymer poly(N-isopropylacrylamide) has received widespread attention for its in vitro application in the non-invasive, non-destructive release of adherent cells on two dimensional surfaces. In this study, 3D non-woven scaffolds fabricated from poly(propylene) (PP), poly(ethylene terephthalate) (...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Rossouw CL,Chetty A,Moolman FS,Birkholtz LM,Hoppe H,Mancama DT

    更新日期:2012-08-01 00:00:00

  • Heat and mass transfer effects in static solid-substrate fermentations: design of fermentation chambers.

    abstract::An experimental device was constructed to allow nearly simultaneous measurements to be made on temperature and gas composition at different depths in a solid-substrate fermentation bed. The time-dependent values of temperature, mol % O(2) and mol % CO(2) were measured at five positions in beds 6.35 cm (2.5 in.) deep. ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Rathbun BL,Shuler ML

    更新日期:1983-04-01 00:00:00

  • Operation of mixed-culture immobilized cell reactors for the metabolism of meta- and para-nitrobenzoate by comamonas sp. JS46 and comamonas sp. JS47

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Goodall JL,Thomas SM,Spain JC,Peretti SW

    更新日期:1998-07-05 00:00:00

  • Design of expression systems for metabolic engineering: coordinated synthesis and degradation of glycogen.

    abstract::In metabolic engineering, systems which allow coordinated control of two metabolic pathways can be useful. We designed two expression systems and demonstrated their application by coordinating glycogen synthesis and degradation. The first expression vector pMSW2 expressed the glycogen synthesis genes in one operon and...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Dedhia N,Chen W,Bailey JE

    更新日期:1997-07-20 00:00:00

  • Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains.

    abstract::A novel fed-batch approach for the production of L-phenylalanine (L-Phe) with recombinant E. coli is presented concerning the on-line control of the key fermentation parameters glucose and tyrosine. Two different production strains possessing either the tyrosine feedback resistant aroF(fbr) (encoding tyrosine feedback...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Gerigk M,Bujnicki R,Ganpo-Nkwenkwa E,Bongaerts J,Sprenger G,Takors R

    更新日期:2002-12-30 00:00:00

  • Improvement of baculovirus as protein expression vector and as biopesticide by CRISPR/Cas9 editing.

    abstract::The clustered regularly interspaced short palindromic repeats (CRISPR) system-associated Cas9 endonuclease is a molecular tool that enables specific sequence editing with high efficiency. In this study, we have explored the use of CRISPR/Cas9 system for the engineering of baculovirus. We have shown that the delivering...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Pazmiño-Ibarra V,Mengual-Martí A,Targovnik AM,Herrero S

    更新日期:2019-11-01 00:00:00

  • Kinetic modeling of oligosaccharide synthesis catalyzed by leuconostoc mesenteroides NRRL B-1299 dextransucrase

    abstract::The kinetic behavior of soluble and insoluble forms of dextransucrase from Leuconostoc mesenteroides NRRL B-1299 was investigated with sucrose as substrate and maltose as acceptor. To study the parameters involved, a kinetic model was applied that was previously developed for L. mesenteroides NRRL B-512F dextransucras...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Dols M,Remaud-Simeon M,Willemot RM,Demuth B,Jordening HJ,Buchholz K,Monsan P

    更新日期:1999-05-05 00:00:00

  • A new bioproduction route for a novel antimicrobial peptide.

    abstract::Beta defensins are antimicrobial peptides (AMPs) with a broad spectrum antimicrobial behavior against pathogens while having minimal tendency to incur pathogen resistance. Human β-defensin 28 (hBD28) is a strongly cationic AMP and hence hypothesized to be highly effective in permeabilizing negatively-charged pathogen ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Tay DK,Rajagopalan G,Li X,Chen Y,Lua LH,Leong SS

    更新日期:2011-03-01 00:00:00

  • Glucagon-induced self-association of recombinant proteins in Escherichia coli and affinity purification using a fragment of glucagon receptor.

    abstract::The specific molecular interactions of alpha-helical peptide, human glucagon (i.e., intermolecular self-association and specific receptor-binding affinity) provided a rationale for using the glucagon as the fusion expression partner to achieve high productivity of foreign proteins both in vivo (in bacterial fusion-exp...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kim DY,Lee J,Saraswat V,Park YH

    更新日期:2000-08-20 00:00:00

  • Thiosulphate improves yield of hydrogen production from glucose by the immobilized formate hydrogenlyase system of Escherichia coli.

    abstract::Escherichia coli cells with formate hydrogenlyase activity that were immobilized in agar beads produced hydrogen from glucose at the approximate yield of 0.6 mole of hydrogen per mole. Succinate or thiosulphate added to glucose increased hydrogen production two-fold. Thiosulphate did not increase the rate of hydrogen ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Nandi R,Dey S,Sengupta S

    更新日期:2001-11-20 00:00:00

  • Analysis of a framework using material balances in metabolic pathways to elucidate cellular metabolism.

    abstract::A framework using material balances in metabolic pathways to study cellular metabolism is examined and the results are discussed. Rate measurements on extracellular compounds alone were found to be not always sufficient to validate proposed unique intracellular mechanisms. The conditions to delineate among candidate m...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Niranjan SC,San KY

    更新日期:1989-08-05 00:00:00

  • Comparative metabolite analysis to understand lactate metabolism shift in Chinese hamster ovary cell culture process.

    abstract::A metabolic shift from lactate production (LP) to net lactate consumption (LC) phenotype was observed in certain Chinese hamster ovary (CHO) cell lines during the implementation of a new chemically defined medium (CDM) formulation for antibody production. In addition, this metabolic shift typically leads to process pe...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Luo J,Vijayasankaran N,Autsen J,Santuray R,Hudson T,Amanullah A,Li F

    更新日期:2012-01-01 00:00:00

  • Physiological responses of Saccharomyces cerevisiae to industrially relevant conditions: Slow growth, low pH, and high CO2 levels.

    abstract::Engineered strains of Saccharomyces cerevisiae are used for industrial production of succinic acid. Optimal process conditions for dicarboxylic-acid yield and recovery include slow growth, low pH, and high CO2 . To quantify and understand how these process parameters affect yeast physiology, this study investigates in...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Hakkaart X,Liu Y,Hulst M,El Masoudi A,Peuscher E,Pronk J,van Gulik W,Daran-Lapujade P

    更新日期:2020-03-01 00:00:00

  • Higher expression of Fab antibody fragments in a CHO cell line at reduced temperature.

    abstract::A chimeric Fab was expressed in Chinese hamster ovary cells under the control of the CMV promoter in a two-stage production process. Cells were first grown to 90% confluence at 37 degrees C in a proliferation phase, followed by a production phase at either 37 degrees C or 28 degrees C. Medium supplemented with serum a...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Schatz SM,Kerschbaumer RJ,Gerstenbauer G,Kral M,Dorner F,Scheiflinger F

    更新日期:2003-11-20 00:00:00

  • Experimental optimization of a real time fed-batch fermentation process using Markov decision process.

    abstract::This article describes a methodology that implements a Markov decision process (MDP) optimization technique in a real time fed-batch experiment. Biological systems can be better modeled under the stochastic framework and MDP is shown to be a suitable technique for their optimization. A nonlinear input/output model is ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Saucedo VM,Karim MN

    更新日期:1997-07-20 00:00:00

  • The influence of dissolved oxygen tension on the synthesis of the antibiotic difficidin by bacillus subtilis.

    abstract::The antibiotic, difficidin, and its hydroxylated derivative oxydifficidin, were synthesized by cultures of Bacillus subtilis grown on a complex medium. Maximum titers of about 200 and 130 mg/L, respectively, were obtained. In fermentations where the dissolved oxygen tension (DOT) was controlled, the maximum specific g...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Suphantharika M,Ison AP,Lilly MD,Buckland BC

    更新日期:1994-10-01 00:00:00

  • Impact of clarification strategy on chromatographic separations: Pre-processing of cell homogenates.

    abstract::This research focused on how the extent and type of primary solid-liquid separation can affect the performance of guard filtration and chromatography, in this instance hydrophobic interaction chromatography. The system used in the study was yeast (Saccharomyces cerevisiae) with the target molecule being an intracellul...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Bracewell DG,Boychyn M,Baldascini H,Storey SA,Bulmer M,More J,Hoare M

    更新日期:2008-08-01 00:00:00

  • Fabrication of nanofibers with antimicrobial functionality used as filters: protection against bacterial contaminants.

    abstract::A comparative study of antimicrobial activity is done using three different electrospun nanofibers namely-CA, PAN, and PVC used as control and with various amounts of AgNO(3) being treated with UV-irradiation leading to the enhancement of silver nanoparticles. DMF is used as the common solvent which helps to undergo s...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Lala NL,Ramaseshan R,Bojun L,Sundarrajan S,Barhate RS,Ying-Jun L,Ramakrishna S

    更新日期:2007-08-15 00:00:00

  • Enzymatic glucosylation of hydrophobic alcohols in organic medium by the reverse hydrolysis reaction using almond-beta-D-glucosidase.

    abstract::Alkyl beta-D-glucosides were synthesized from D-glucose and alcohols by reverse hydrolysis using the commercially available almond beta-D-glucosidase in 9:1 (v/v) acetonitrile-water medium. The main characteristics of this enzyme-catalyzed glucosylation were established by using 2-hydroxybenzyl alcohol. The reaction i...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Vic G,Biton J,Le Beller D,Michel JM,Thomas D

    更新日期:1995-04-20 00:00:00

  • Glucose oxidase stabilization against thermal inactivation using high hydrostatic pressure and hydrophobic modification.

    abstract::High hydrostatic pressure (HHP) stabilized glucose oxidase (GOx) against thermal inactivation. The apparent first-order kinetics of inactivation of GOx were investigated at 0.1-300 MPa and 58.8-80.0°C. At 240 MPa and 74.5°C, GOx inactivated at a rate 50 times slower than at atmospheric pressure at the same temperature...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Halalipour A,Duff MR Jr,Howell EE,Reyes-De-Corcuera JI

    更新日期:2017-03-01 00:00:00

  • Optimizing normoxic conditions in liver devices using enhanced gel matrices.

    abstract::For in vitro liver replacement devices, such as packed bed bioreactors, to maintain the essential functions of the liver, they must at least successfully support hepatocytes, the parenchymal cell of the liver. In vivo, the liver is a major consumer of oxygen. Hence it is unsurprising that the limited transport distanc...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Niu M,Clemens MG,Coger RN

    更新日期:2008-04-15 00:00:00