Chemotaxis increases vertical migration and apparent transverse dispersion of bacteria in a bench-scale microcosm.

abstract：:Pretreatment plays an important role in the efficient enzymatic hydrolysis of biomass into fermentable sugars for biofuels. A highly effective pretreatment method is reported for corn stover which combines mild alkali-extraction followed by ionic liquid (IL) dissolution of the polysaccharides and regeneration (recover...

journal_title：Biotechnology and bioengineering

authors： Geng X,Henderson WA

更新日期：2012-01-01 00:00:00

abstract：:A newly designed batch calorimeter was used to investigate the degradability of some celluloses having varying degrees of crystallinity. The PTC of an aerobic culture of Cellulomonas uda ATCC 21399 obtained revealed a diauxic growth which is attributed to the presence of hemicellulose contaminating Avicel and MN300 ce...

journal_title：Biotechnology and bioengineering

authors： Dermoun Z,Belaich JP

更新日期：1985-07-01 00:00:00

abstract：:The development of autoantibodies against factor VIII represents one of the major complications in the treatment of hemophilia A patients. We have employed a novel library system to obtain peptides that specifically neutralize the interaction between factor VIII and these inhibitors. The random peptides are presented ...

journal_title：Biotechnology and bioengineering

authors： Mersich C,Billes W,Pabinger I,Jungbauer A

更新日期：2007-09-01 00:00:00

abstract：:The diffusion characteristics of sucrose, a nutrient, and yohimbine, a secondary metabolite, in alginate gel beads, with or without entrapped periwinkle (Catharanthus roseus) or apple (Malus domestica) cells, were investigated. Effective diffusivities of both solutes in the gel beads were determined by two different m...

journal_title：Biotechnology and bioengineering

authors： Pu HT,Yang RY

更新日期：1988-09-20 00:00:00

abstract：:Water permeability (Lp), calculated from the volume variations of cells subjected to an osmotic shock, is classically used to characterize cell membrane properties. In this work, we have shown the importance of the kind of mixing reactor used to measure the Lp parameter. A mathematical model including the mixing time ...

journal_title：Biotechnology and bioengineering

authors： de Marañón IM,Gervais P,Molin P

更新日期：1997-10-05 00:00:00

abstract：:Membrane adsorbers may be a viable alternative to the packed-bed chromatography for clearance of virus, host cell proteins, DNA, and other trace impurities. However, incorporation of membrane adsorbers into manufacturing processes has been slow due to the significant cost associated with obtaining regulatory approval ...

journal_title：Biotechnology and bioengineering

authors： Weaver J,Husson SM,Murphy L,Wickramasinghe SR

更新日期：2013-02-01 00:00:00

abstract：:Macroencapsulation technology has been an attractive topic in the field of treatment for Type 1 diabetes due to mechanical stability, versatility, and retrievability of the macro-capsule design. Macro-capsules can be categorized into extravascular and intravascular devices, in which solute transport relies either on d...

journal_title：Biotechnology and bioengineering

authors： Song S,Roy S

更新日期：2016-07-01 00:00:00

abstract：:Ethanol-producing bioreactors employing cells of Zymomonas mobilis attached to glass-fiber pads were operated continuously for as long as 28 days. Ethanol production, which is related to bed-associated biomass levels, was found to occur in three distinct phases: an exponential phase, a linear phase, and a "steady-stat...

journal_title：Biotechnology and bioengineering

authors： Arcuri EJ

更新日期：1982-03-01 00:00:00

abstract：:A three-pool growth model of an individual Escherichia coli cell is described herein. The model is based on a previously developed chemically structured complex single cell growth model. The reduction in model complexity and the identification of the essential modes of motion, over the time scale of growth, is achieve...

journal_title：Biotechnology and bioengineering

authors： Joshi A,Palsson BO

更新日期：1988-02-05 00:00:00

abstract：:In enzymatic lysis of yeast for the recovery of intracellular proteins, the rupture of whole cells is caused by the action of a lytic system consisting primarily of protease and glucanase. A first-principles mechanism for the lytic reaction based on a two-layer model of the wall structure and a burst model for the dis...

journal_title：Biotechnology and bioengineering

authors： Liu LC,Prokopakis GJ,Asenjo JA

更新日期：1988-10-20 00:00:00

abstract：:Nitrifying bacteria, cyanobacteria, and algae are important microorganisms in open pond wastewater treatment systems. Nitrification involving the sequential oxidation of ammonia to nitrite and nitrate, mainly due to autotrophic nitrifying bacteria, is essential to biological nitrogen removal in wastewater and global n...

journal_title：Biotechnology and bioengineering

authors： Choi O,Das A,Yu CP,Hu Z

更新日期：2010-12-15 00:00:00

abstract：:A low-density, porous chitosan/poly-(dl-lactide-co-glycolide) (PLGA) microparticle composite scaffold was produced by thermally induced phase separation followed by lyophilization, to provide a bicontinuous microstructure potentially suitable for tissue engineering and locally controlled drug release. PLGA particles w...

journal_title：Biotechnology and bioengineering

authors： Nygaard JV,Andersen MØ,Howard KA,Foss M,Bünger C,Kjems J,Besenbacher F

更新日期：2008-07-01 00:00:00

abstract：:Two thermophilic bacteria, Thermus aquaticus ATCC 25104 and Thermus species ATCC 27978, were investigated for their abilities to degrade BTEX (benzene, toluene, ethylbenzene, and xylenes). Thermus aquaticus and the Thermus sp. were grown in a nominal medium at 70 degrees C and 60 degrees C, respectively, and resting c...

journal_title：Biotechnology and bioengineering

authors： Chen CI,Taylor RT

更新日期：1995-12-20 00:00:00

abstract：:The damaging and degenerative effects in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and Crohn's disease often manifests as the formation of lesions that feature a high local concentration of granulocyte-macrophage colony-stimulating factor (GM-CSF). GM-CSF along with other pro-inflammatory fa...

journal_title：Biotechnology and bioengineering

authors： Mosabbir AA,Qudrat A,Truong K

更新日期：2018-04-01 00:00:00

abstract：:Numerous high-value proteins are secreted into the Escherichia coli periplasm by the General Secretory (Sec) pathway, but Sec-based production chassis cannot handle many potential target proteins. The Tat pathway offers a promising alternative because it transports fully folded proteins; however, yields have been too ...

journal_title：Biotechnology and bioengineering

authors： Browning DF,Richards KL,Peswani AR,Roobol J,Busby SJW,Robinson C

更新日期：2017-12-01 00:00:00

abstract：:A spinning cylindrical filter is often used to retain mammalian cells in a continuous perfusion bioreactor. This device, known as a spinfilter, has typically been with pore size smaller than the cell particles (single cells or aggregates) in order to achieve cell separation. For single cells in suspension, such an ope...

journal_title：Biotechnology and bioengineering

authors： Yabannavar VM,Singh V,Connelly NV

更新日期：1992-10-20 00:00:00

abstract：:Retroviral-derived biopharmaceuticals (RV) target numerous therapeutic applications, from gene therapy to virus-like particle (rVLP)-based vaccines. During particle formation, beside the pseudotyped envelope proteins, RV can incorporate proteins derived from the virus producer cells (VPC). This may be detrimental by r...

journal_title：Biotechnology and bioengineering

authors： Rodrigues AF,Guerreiro MR,Santiago VM,Dalba C,Klatzmann D,Alves PM,Carrondo MJ,Coroadinha AS

更新日期：2011-11-01 00:00:00

abstract：:Liquid membrane bilayers, generated by bacteriorhodopsin on a supporting membrane, exhibit photo osmosis. The phenomenon has been shown to be a consequence of light-induced electrical potential differences which develop across the liquid membrane bilayer due to the light-driven proton pumping action of bacteriorhodops...

journal_title：Biotechnology and bioengineering

authors： Srivastava RC,Tandon A

更新日期：1988-04-20 00:00:00

abstract：:Purified rat liver phenylalanine hydroxylase [L-phenylalanine:tetrahydropteridine:oxygen oxidoreductase (4-hydroxylating), EC 1.14.16.1] was immobilized with activated thiol-Sepharose 4B via disulfide bond formation, which is expected to immobilize the enzyme in its activated form through the SH modification. This imm...

journal_title：Biotechnology and bioengineering

authors： Koizumi S,Takeuchi T,Umezawa H,Nagatsu T

更新日期：1986-04-01 00:00:00

abstract：:This work investigated the use of nuclear magnetic resonance (NMR) spectroscopy in combination with a mathematical model of an encapsulated cell system as a method for rapidly assessing the status of a pancreatic substitute. To validate this method, an in vitro experiment was performed in which the encapsulated cells ...

journal_title：Biotechnology and bioengineering

authors： Gross JD,Long RC Jr,Constantinidis I,Sambanis A

更新日期：2007-09-01 00:00:00

abstract：:Cell-division-cycle, temperature-sensitive mutants of Saccharomyces cerevisiae were investigated as a means of altering the morphological characteristics and subsequent physical properties of single-cell protein (SCP). Strain 4471, harboring mutation cdc 4, formed a visible complex mass at the nonpermissive temperatur...

journal_title：Biotechnology and bioengineering

authors： Miyasaka Y,Rha C,Sinskey AJ

更新日期：1980-10-01 00:00:00

abstract：:This reports on the determination of the overall oxygen transfer coefficient in a mechanically agitated vessel using a randomly pulsed dynamic method. This method consists in exciting the system by randomly switching the inlet gas stream with air or nitrogen with an identical volumetric flow rate. A pseudo-random bina...

journal_title：Biotechnology and bioengineering

authors： Gauthier L,Thibault J,LeDuy A

更新日期：1991-04-15 00:00:00

abstract：:Mixed-culture fermentation is a key central process to enable next generation biofuels and biocommodity production due to economic and process advantages over application of pure cultures. However, a key limitation to the application of mixed-culture fermentation is predicting culture product response, related to meta...

journal_title：Biotechnology and bioengineering

authors： Hoelzle RD,Virdis B,Batstone DJ

更新日期：2014-11-01 00:00:00

abstract：:Thermochemical pretreatment and enzymatic hydrolysis are the areas contributing most to the operational costs of second generation ethanol in lignocellulosic biorefineries. The improvement of lignocellulosic enzyme cocktails has been significant in the recent years. Although the needs for the reduction of the energy i...

journal_title：Biotechnology and bioengineering

authors： Martín Pérez L,Benítez Casanova L,Moreno Pérez AJ,Pérez Gómez D,Gavaldá Martín S,Ledesma-García L,Valbuena Crespo N,Díez García B,Reyes-Sosa FM

更新日期：2017-11-01 00:00:00

abstract：:Equilibrium adsorption studies on Escherichia coli and Saccharomyces sp. revealed the capacity and affinity of these organisms for the surfaces of powdered charcoal and nickel. In simple salt solutions both organisms readily adsorbed to each solid with an affinity and maximum loading capacity individual to each cell-s...

journal_title：Biotechnology and bioengineering

authors： Protheroe RG,Cumming RH,Matchett A

更新日期：1989-10-05 00:00:00

abstract：:A framework using material balances in metabolic pathways to study cellular metabolism is examined and the results are discussed. Rate measurements on extracellular compounds alone were found to be not always sufficient to validate proposed unique intracellular mechanisms. The conditions to delineate among candidate m...

journal_title：Biotechnology and bioengineering

authors： Niranjan SC,San KY

更新日期：1989-08-05 00:00:00

abstract：:This article reports a study of the performance of membrane-attached biofilms grown in a single tube extractive membrane bioreactor (STEMS) used for the treatment of a synthetic wastewater containing a toxic VOC (1,2-dichloroethane [DCE]). Mass balances show that complete mineralization of DCE was achieved, and that t...

journal_title：Biotechnology and bioengineering

authors： Dos Santos LM,Livingston AG

更新日期：1995-07-05 00:00:00

abstract：:We report the isolation of mutant strains of the methylotrophic yeast Hansenula polymorpha that are able to efficiently oxidize ethanol to acetaldehyde in an intact cell system. The oxidation reaction is catalyzed by alcohol oxidase (AOX), a key enzyme in the methanol metabolic pathway that is typically present only i...

journal_title：Biotechnology and bioengineering

authors： Moroz OM,Gonchar MV,Sibirny AA

更新日期：2000-04-05 00:00:00

abstract：:The addition of simple inorganic salts to aqueous enzyme solutions prior to lyophilization results in a dramatic activation of the dried powder in organic media relative to enzyme with no added salt. Activation of both the serine protease subtilisin Carlsberg and lipase from Mucor javanicus resulting from lyophilizati...

journal_title：Biotechnology and bioengineering

authors： Ru MT,Dordick JS,Reimer JA,Clark DS

更新日期：1999-04-20 00:00:00

abstract：:Human tissue-type plasminogen activator (t-PA) contains a variably occupied glycosylation site at Asn-184 in naturally produced t-PA and in t-PA produced in recombinant Chinese hamster ovary (CHO) cells. The presence of an oligosaccharide at this site has previously been shown to reduce specific activity and fibrin bi...

journal_title：Biotechnology and bioengineering

authors： Andersen DC,Bridges T,Gawlitzek M,Hoy C

更新日期：2000-10-05 00:00:00