Modeling the nutrient removal process in aerobic granular sludge system by coupling the reactor- and granule-scale models.

abstract：:PER.C6 cells were cultivated for propagation of a replication-defective adenovirus vector in serum-free suspension bioreactors. Cellular metabolism during cell growth and adenovirus propagation was fully characterized using on-line and off-line methods. The energy metabolism was found to accelerate transiently after a...

journal_title：Biotechnology and bioengineering

authors： Maranga L,Auniņs JG,Zhou W

更新日期：2005-06-05 00:00:00

abstract：:Bifidobacterium animalis ATCC 27536 represents among bifidobacteria a host-model for cloning experiments. The segregational and structural stabilities of a family of cloning vectors with different molecular weights but sharing a common core were studied in continuous fermentation of the hosting B. animalis without sel...

journal_title：Biotechnology and bioengineering

authors： González Vara A,Rossi M,Altomare L,Eikmanns B,Matteuzzi D

更新日期：2003-10-20 00:00:00

abstract：:Hairy roots have the potential to produce a variety of valuable small and large molecules. The mist reactor is a gas phase bioreactor that has shown promise for low-cost culture of hairy roots. Using a newer, disposable culture bag, mist reactor performance was studied with two species, Artemisia annua L. and Arachis ...

journal_title：Biotechnology and bioengineering

authors： Sivakumar G,Liu C,Towler MJ,Weathers PJ

更新日期：2010-12-01 00:00:00

abstract：:A highly active xylose specific transporter without glucose inhibition is highly desirable in cost-effective production of biofuels from lignocellulosic biomass. However, currently available xylose specific transporters suffer from low overall activity and most are inhibited by glucose. In this study, we applied a dir...

journal_title：Biotechnology and bioengineering

authors： Wang M,Yu C,Zhao H

更新日期：2016-03-01 00:00:00

abstract：:Microbial degradation of trichloroethylene (TCE) has been demonstrated under aerobic conditions with propane. The primary objective of this research was to evaluate the feasibility of introducing a vapor phase form of TCE in the presence of propane to batch bioreactors containing a liquid phase suspension of Mycobacte...

journal_title：Biotechnology and bioengineering

authors： Wilcox DW,Autenrieth RL,Bonner JS

更新日期：1995-05-20 00:00:00

abstract：:A mechanistic model-based soft sensor is developed and validated for 550L filamentous fungus fermentations operated at Novozymes A/S. The soft sensor is comprised of a parameter estimation block based on a stoichiometric balance, coupled to a dynamic process model. The on-line parameter estimation block models the cha...

journal_title：Biotechnology and bioengineering

authors： Mears L,Stocks SM,Albaek MO,Sin G,Gernaey KV

更新日期：2017-03-01 00:00:00

abstract：:In this study we describe a model that estimates the extracellular (nonfungal) and overall water contents of wheat grains during solid-state fermentation (SSF) with Aspergillus oryzae, using on-line measurements of oxygen, carbon dioxide, and water vapor in the gas phase. The model uses elemental balances to predict s...

journal_title：Biotechnology and bioengineering

authors： Nagel FJ,Tramper J,Bakker MS,Rinzema A

更新日期：2001-01-20 00:00:00

abstract：:NS0, a nonsecreting mouse myeloma cell, is a major host line used for recombinant antibody production. These cells have a cholesterol-dependent phenotype and rely on an exogenous supply of cholesterol for their survival and growth. To better understand the physiology underlying cholesterol dependence, we compared NS0 ...

journal_title：Biotechnology and bioengineering

authors： Seth G,Philp RJ,Denoya CD,McGrath K,Stutzman-Engwall KJ,Yap M,Hu WS

更新日期：2005-06-05 00:00:00

abstract：:Models for the known glucose transporters in Aspergillus niger and for simple diffusion of glucose through the hyphal membrane were prepared. The results from the models were compared with fermentation data from published studies on citric acid. It was found that the purely physical and uncontrolled process of diffusi...

journal_title：Biotechnology and bioengineering

authors： Wayman FM,Mattey M

更新日期：2000-02-20 00:00:00

abstract：:To monitor gas reaction rates in animal cell culture at constant dissolved oxygen concentration (DO) and constant pH it was necessary to develop improved control methods. Decoupling of both controllrs was obtained by manipulation of molar fractions of oxygen and carbon dioxide in the gas phase. Two pairs of DO and pH ...

journal_title：Biotechnology and bioengineering

authors： Oeggerli A,Eyer K,Heinzle E

更新日期：1995-01-05 00:00:00

abstract：:Bacterial magnetic particles (BMPs) were used for the identification of cyanobacterial DNA. Genus-specific oligonucleotide probes for the detection of Anabaena spp., Microcystis spp., Nostoc spp., Oscillatoria spp., and Synechococcus spp. were designed from the variable region of the cyanobacterial 16S rDNA of 148 str...

journal_title：Biotechnology and bioengineering

authors： Matsunaga T,Nakayama H,Okochi M,Takeyama H

更新日期：2001-06-05 00:00:00

abstract：:Animal cell culture is widely used in biotechnology for the production of many biological products. In situ microscopes acquire images directly from cell suspensions and analyze the images in matters of cell concentration, cell size distribution and cell morphology. Their applicability was already proven for yeast and...

journal_title：Biotechnology and bioengineering

authors： Rudolph G,Lindner P,Gierse A,Bluma A,Martinez G,Hitzmann B,Scheper T

更新日期：2008-01-01 00:00:00

abstract：:An extractive acetonobutylic fermentation process is developed by integrating bioproduction, Ultrafiltration, and distillation, providing simultaneous retention of biomass, selective removal of inhibitors from the permeate, as well as separation and purification of acetone-butanol-ethanol solvents. Successive batch fe...

journal_title：Biotechnology and bioengineering

authors： Minier M,Grateloup R,Blanc-Ferras E,Goma G

更新日期：1990-04-15 00:00:00

abstract：:A new ultrafiltration technique based on a multimembrane stack has been developed to fractionate solutes closer in size than conventionally possible. The technique is illustrated here by obtaining a pure protein product from a binary protein mixture. By employing membranes in series without any gaskets or spacers in-b...

journal_title：Biotechnology and bioengineering

authors： Feins M,Sirkar KK

更新日期：2004-06-20 00:00:00

abstract：:One unique to detect cytotoxicity is to utilize reporter gene assays for promoters that respond to stress-induced effects. In the present study, we discovered that the DNA sequence from nt -287 to +110 of the heat shock protein 70B' (HSP70B') gene could be used as a functional promoter to detect cytotoxicity of cadmiu...

journal_title：Biotechnology and bioengineering

authors： Wada K,Taniguchi A,Xu L,Okano T

更新日期：2005-11-20 00:00:00

abstract：:Recombinant protein glycosylation profiles have been shown to affect the in-vivo half-life, and therefore the efficacy and economics, for many therapeutics. While much research has been conducted correlating the effects of various stimuli on recombinant protein glycosylation characteristics, relatively little work has...

journal_title：Biotechnology and bioengineering

authors： Clark KJ,Griffiths J,Bailey KM,Harcum SW

更新日期：2005-06-05 00:00:00

abstract：:Chondral and osteochondral lesions represent one of the most challenging problems in the orthopedic field, as these types of injuries lead to disability and worsened quality of life for patients and have an economic impact on the healthcare system. The aim of this in vivo study was to develop a new tissue engineering ...

journal_title：Biotechnology and bioengineering

authors： Tschon M,Brogini S,Parrilli A,Bertoldi S,Silini A,Parolini O,Faré S,Martini L,Veronesi F,Fini M,Giavaresi G

更新日期：2021-01-01 00:00:00

abstract：:Electron paramagnetic resonance spectroscopy is used to observe hydroxyl radicals produced by an atmospheric pressure nonthermal plasma device at distances greater than 1 m from the discharge. The plasma device is an indirect treatment setup with closed loop airflow and hydrogen peroxide additives that is effective in...

journal_title：Biotechnology and bioengineering

authors： Plimpton SR,Gołkowski M,Mitchell DG,Austin C,Eaton SS,Eaton GR,Gołkowski C,Voskuil M

更新日期：2013-07-01 00:00:00

abstract：:Derivatives of Escherichia coli C were engineered to produce primarily succinate or malate in mineral salts media using simple fermentations (anaerobic stirred batch with pH control) without the addition of plasmids or foreign genes. This was done by a combination of gene deletions (genetic engineering) and metabolic ...

journal_title：Biotechnology and bioengineering

authors： Jantama K,Haupt MJ,Svoronos SA,Zhang X,Moore JC,Shanmugam KT,Ingram LO

更新日期：2008-04-01 00:00:00

abstract：:C-terminal lysine (C-K) variants are commonly observed in therapeutic monoclonal antibodies and recombinant proteins. Heterogeneity of C-K residues is believed to result from varying degree of proteolysis by endogenous carboxypeptidase(s) during cell culture production. The achievement of batch-to-batch culture perfor...

journal_title：Biotechnology and bioengineering

authors： Luo J,Zhang J,Ren D,Tsai WL,Li F,Amanullah A,Hudson T

更新日期：2012-09-01 00:00:00

abstract：:Cell-based tissue engineering is limited by the size of cell-containing constructs that can be successfully cultured in vitro. This limit is largely a result of the slow diffusion of molecules such as oxygen into the interior of three-dimensional scaffolds in static culture. Bioreactor culture has been shown to overco...

journal_title：Biotechnology and bioengineering

authors： Yeatts AB,Geibel EM,Fears FF,Fisher JP

更新日期：2012-09-01 00:00:00

abstract：:MicroRNAs are small non-coding RNAs that play a critical role in post-transcriptional control of gene expression. Recent publications of genomic sequencing data from the Chinese Hamster (CGR) and Chinese hamster ovary (CHO) cells provide new tools for the discovery of novel miRNAs in this important production system. ...

journal_title：Biotechnology and bioengineering

authors： Diendorfer AB,Hackl M,Klanert G,Jadhav V,Reithofer M,Stiefel F,Hesse F,Grillari J,Borth N

更新日期：2015-07-01 00:00:00

abstract：:The knowledge of critical process-relevant genes can be used for an improved control of bioprocesses. So far bioprocess-relevant marker genes can be analyzed by established expression analysis methods only off-line. In this study, an alternative approach for a potential at-line monitoring of gene expression during bio...

journal_title：Biotechnology and bioengineering

authors： Jürgen B,Barken KB,Tobisch S,Pioch D,Wümpelmann M,Hecker M,Schweder T

更新日期：2005-11-05 00:00:00

abstract：:Alcoholic fermentation of cheese whey permeate was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for beta-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus enabling for lactose metabolization. Data on yeast fermentation and gro...

journal_title：Biotechnology and bioengineering

authors： Domingues L,Lima N,Teixeira JA

更新日期：2001-03-05 00:00:00

abstract：:3-Dehydroshikimic acid (DHS), in addition to being a potent antioxidant, is the key hydroaromatic intermediate in the biocatalytic conversion of glucose into aromatic bioproducts and a variety of industrial chemicals. Microbial synthesis of DHS, like other intermediates in the common pathway of aromatic amino acid bio...

journal_title：Biotechnology and bioengineering

authors： Li K,Mikola MR,Draths KM,Worden RM,Frost JW

更新日期：1999-07-05 00:00:00

abstract：:The diffusion characteristics of sucrose, a nutrient, and yohimbine, a secondary metabolite, in alginate gel beads, with or without entrapped periwinkle (Catharanthus roseus) or apple (Malus domestica) cells, were investigated. Effective diffusivities of both solutes in the gel beads were determined by two different m...

journal_title：Biotechnology and bioengineering

authors： Pu HT,Yang RY

更新日期：1988-09-20 00:00:00

abstract：:A modified mixing plane approach for steady state simulation of flow field in fully baffled biological reactor is presented and discussed. Without requiring any experimental input, this approach of dividing the vessel into suitable number of connected and disconnected zones; solving steady state equation separately in...

journal_title：Biotechnology and bioengineering

authors： Dubey H,Das SK,Panda T

更新日期：2006-11-05 00:00:00

abstract：:Self-cycling fermentation (SCF), a cyclical, semi-continuous process that induces cell synchrony, was incorporated into a recombinant protein production scheme. Escherichia coli CY15050, a lac(-) mutant lysogenized with temperature-sensitive phage λ modified to over-express β-galactosidase, was used as a model system....

journal_title：Biotechnology and bioengineering

authors： Storms ZJ,Brown T,Sauvageau D,Cooper DG

更新日期：2012-09-01 00:00:00

abstract：:A recently discovered thermophilic bacterium, Geobacillus thermoglucosidasius M10EXG, ferments a range of C5 (e.g., xylose) and C6 sugars (e.g., glucose) and is tolerant to high ethanol concentrations (10%, v/v). We have investigated the central metabolism of this bacterium using both in vitro enzyme assays and 13C-ba...

journal_title：Biotechnology and bioengineering

authors： Tang YJ,Sapra R,Joyner D,Hazen TC,Myers S,Reichmuth D,Blanch H,Keasling JD

更新日期：2009-04-01 00:00:00

abstract：:Buffered aqueous two-phase systems are effective extraction systems for separating amphoteric hydrocarbons like, for example, polypeptides from aqueous phases. The design and basic engineering of such processes requires the knowledge of the liquid-liquid equilibrium. The study presented here aims to contribute to the ...

journal_title：Biotechnology and bioengineering

authors： Grossmann C,Tintinger R,Zhu J,Maurer G

更新日期：1998-12-20 00:00:00