Diffusion of sucrose and yohimbine in calcium alginate gel beads with or without entrapped plant cells.

abstract：:Two empirical models for the enzymatic hydrolysis of cellulose are used to analyze the same set of experimental data to determine if one model is superior to the other. Both models adequately describe the data. The parameters in both models may be correlated to the hydrolysis conditions of enzyme, substrate, and produ...

journal_title：Biotechnology and bioengineering

authors： Holtzapple MT,Caram HS,Humphrey AE

更新日期：1984-08-01 00:00:00

abstract：:A respiratory-deficient, mutant of Kluyveromyces fragilis was isolated using a ethidium bromide mutagenesis. It was characterized by a loss of cytochromes a + a3 and by an improvement of its inulinase activity. Under anaerobic conditions this mutant was always better than the wild strain for ethanol production especia...

journal_title：Biotechnology and bioengineering

authors： Guiraud JP,Bourgi J,Stervinou M,Claisse M,Galzy P

更新日期：1987-05-01 00:00:00

abstract：:Osteochondral tissue engineering aims to regenerate functional tissue-mimicking physiological properties of injured cartilage and its subchondral bone. Given the distinct structural and biochemical difference between bone and cartilage, bilayered scaffolds, and bioreactors are commonly employed. We present an osteocho...

journal_title：Biotechnology and bioengineering

authors： Xue R,Chung B,Tamaddon M,Carr J,Liu C,Cartmell SH

更新日期：2019-11-01 00:00:00

abstract：:Trichoplusia ni larvae were infected with baculoviruses containing genes for the expression of ultraviolet optimized green fluorescent protein (GFPuv) and several product proteins. A GFP-specific optical probe was used to both excite the green fluorescent protein (lambda(ex) = 385 nm), and subsequently monitor fluores...

journal_title：Biotechnology and bioengineering

authors： Kramer SF,Kostov Y,Rao G,Bentley WE

更新日期：2003-07-20 00:00:00

abstract：:Mevalonate is a useful metabolite synthesized from three molecules of acetyl-CoA, consuming two molecules of NADPH. Escherichia coli ( E. coli) catabolizes glucose to acetyl-CoA via several routes, such as the Embden-Meyerhof-Parnas (EMP) and the oxidative pentose phosphate (oxPP) pathways. Although the oxPP pathway s...

journal_title：Biotechnology and bioengineering

authors： Kamata K,Toya Y,Shimizu H

更新日期：2019-05-01 00:00:00

abstract：:While feeding protocols and ectopic expression of anti-apoptotic genes have been used to improve the viability of hybridoma cell lines, the effect of the expression levels of survival genes on the behavior of hybridomas following nutrient supplementation is unknown. In this study, we compared the behavior of the Sp2/0...

journal_title：Biotechnology and bioengineering

authors： Charbonneau JR,Furtak T,Lefebvre J,Gauthier ER

更新日期：2003-02-05 00:00:00

abstract：:An automated system with semi on-line monitoring of glucose, lactic acid, protein, and optical density during lactic acid fermentations, is set up to study the dynamics of lactic acid bacteria. The analyzers for glucose, lactic acid, and protein are based on flow injection analysis (FIA). The system consists of a labo...

journal_title：Biotechnology and bioengineering

authors： Nielsen J,Nikolajsen K,Villadsen J

更新日期：1989-04-05 00:00:00

abstract：:Oxygenases catalyze, among other interesting reactions, highly selective hydrocarbon oxyfunctionalizations, which are important in industrial organic synthesis but difficult to achieve by chemical means. Many enzymatic oxygenations have been described, but few of these have been scaled up to industrial scales, due to ...

journal_title：Biotechnology and bioengineering

authors： Bühler B,Bollhalder I,Hauer B,Witholt B,Schmid A

更新日期：2003-06-30 00:00:00

abstract：:Whole cell pyruvate decarboxylase (PDC) from Candida utilis enhanced the enzymatic production of (R)-phenylacetylcarbinol (PAC) in an aqueous/octanol biotransformation compared to the partially purified PDC especially for a lower range of initial activities (0.3-2.5 U/mL). With an initial activity of 1.1 U/mL and at a...

journal_title：Biotechnology and bioengineering

authors： Satianegara G,Rogers PL,Rosche B

更新日期：2006-08-20 00:00:00

abstract：:Pseudomonas putida cells were grown in confined volumes in dual-membrane immobilized cell reactors constructed from microporous polyethylene hollow fibers and silicone rubber tubules as a model system for the study of mass transport in microbial aggregates. Local cell concentrations in the reactors reached 300 g dry m...

journal_title：Biotechnology and bioengineering

authors： Karel SF,Robertson CR

更新日期：1989-07-01 00:00:00

abstract：:The effect of ligand density on anion-exchange membrane chromatography (AEXmc) for the purification of recombinant baculoviruses (rBVs), potential viral vectors in clinical applications, is studied by surface plasmon resonance on customized AEX surfaces and gradient elution experiments on Sartobind D membrane prototyp...

journal_title：Biotechnology and bioengineering

authors： Vicente T,Fáber R,Alves PM,Carrondo MJ,Mota JP

更新日期：2011-06-01 00:00:00

abstract：:The development of autoantibodies against factor VIII represents one of the major complications in the treatment of hemophilia A patients. We have employed a novel library system to obtain peptides that specifically neutralize the interaction between factor VIII and these inhibitors. The random peptides are presented ...

journal_title：Biotechnology and bioengineering

authors： Mersich C,Billes W,Pabinger I,Jungbauer A

更新日期：2007-09-01 00:00:00

abstract：:To study the influence of ammonium on an antibiotic cultivation, mass transfer measurements of ammonium through microporous hydrophobic membranes using different stripping methods were carried out and compared. The higher overall mass transfer coefficients for ammonium were obtained with an acid stripping solution com...

journal_title：Biotechnology and bioengineering

authors： Hecht V,Bischoff L,Gerth K

更新日期：1990-04-25 00:00:00

abstract：:This study examines protein adsorption behavior and the effects of mobile phase modifiers in multimodal chromatographic systems. Chromatography results with a diverse protein library indicate that multimodal and ion exchange resins have markedly different protein binding behavior and selectivity. NMR results corrobora...

journal_title：Biotechnology and bioengineering

authors： Holstein MA,Parimal S,McCallum SA,Cramer SM

更新日期：2012-01-01 00:00:00

abstract：:Water permeability (Lp), calculated from the volume variations of cells subjected to an osmotic shock, is classically used to characterize cell membrane properties. In this work, we have shown the importance of the kind of mixing reactor used to measure the Lp parameter. A mathematical model including the mixing time ...

journal_title：Biotechnology and bioengineering

authors： de Marañón IM,Gervais P,Molin P

更新日期：1997-10-05 00:00:00

abstract：:Stricter regulations on volatile organic compounds and hazardous air pollutants have increased the demand for abatement technologies. Biofiltration, a process in which contaminated air is passed through a biologically active bed, can be used to remove these pollutants from air streams. In this study, a fungal vapor-ph...

journal_title：Biotechnology and bioengineering

authors： Woertz JR,Kinney KA,McIntosh ND,Szaniszlo PJ

更新日期：2001-12-05 00:00:00

abstract：:A successful procedure for attaching cellulase to 45 microm iron oxide particles with a high-molecular-weight (1000) ligand has been developed. Mass and activity balances were calculated for immobilized enzymes with different loadings. The highest specific activity of the immobilized enzyme was 5.9 mmol glucose/g boun...

journal_title：Biotechnology and bioengineering

authors： Garcia A 3rd,Oh S,Engler CR

更新日期：1989-01-15 00:00:00

abstract：:The specific uptake rate of phenol by washed cells of Pseudomonas putida grown on phenol in steady-state continuous culture at various dilution rates was studied. The Monod-Haldane-type equation was applied to fit the data and the best kinetic parameters were determined by nonlinear least-squares techniques. The value...

journal_title：Biotechnology and bioengineering

authors： Sokol W

更新日期：1987-12-05 00:00:00

abstract：:The hydrolysis of lactose by a beta-galactosidase from the thermophilic microorganism Thermus sp. strain T2, both in solution and immobilized on a commercial silica-alumina, has been studied. The enzyme has been previously produced by Escherichia coli JM101 harboring the plasmid pBGT1, which contains the codifying gen...

journal_title：Biotechnology and bioengineering

authors： Ladero M,Perez MT,Santos A,Garcia-Ochoa F

更新日期：2003-01-20 00:00:00

abstract：:Isothermal microcalorimeters (IMC) are highly sensitive instruments that allow the measurement of heat flow in the microwatt range. Due to their detection of minute thermal heat, IMC techniques have been used in numerous biological applications, including the study of fermentation processes, pharmaceutical development...

journal_title：Biotechnology and bioengineering

authors： Santoro R,Braissant O,Müller B,Wirz D,Daniels AU,Martin I,Wendt D

更新日期：2011-12-01 00:00:00

abstract：:Analytical expressions describing convective flow in a continuous arteriovenous hollow fiber hemofilter were developed. In the lumen of the hollow fiber membrane, existing analytical expressions were applied to describe velocity profiles and pressure. For flow in the shell (the extracapillary space separating the fibe...

journal_title：Biotechnology and bioengineering

authors： Moussy Y

更新日期：2000-04-20 00:00:00

abstract：:A model previously developed to characterize enzymatic in activation behavior was used to explain the non-first-order biphasic and grace period phenomena that are often observed with oligomeric enzymes. Luciferase and urease were used as model enzyme such as luciferase, the oligomer initially dissociates reversibly in...

journal_title：Biotechnology and bioengineering

authors： Lencki RW,Arul J,Neufeld RJ

更新日期：1992-12-20 00:00:00

abstract：:The emissions of nitrous oxide (N(2)O) and nitric oxide (NO) from biological nitrogen removal (BNR) operations via nitrification and denitrification is gaining increased prominence. While many factors relevant to the operation of denitrifying reactors can influence N(2)O and NO emissions from them, the role of differe...

journal_title：Biotechnology and bioengineering

authors： Lu H,Chandran K

更新日期：2010-06-15 00:00:00

abstract：:This paper presents an experimental analysis of matrix bead size distribution and voidage variations with axial height in an expanded bed adsorption system. Use of a specially constructed expanded bed with side ports has enabled sampling from within the expanded bed along the vertical axis. Particles removed from with...

journal_title：Biotechnology and bioengineering

authors： Willoughby NA,Hjorth R,Titchener-Hooker NJ

更新日期：2000-09-20 00:00:00

abstract：:Glucose oxidase, invertase, and amyloglucosidase were entrapped in calcium alginate gels as concanavalin A complexes in order to prevent the leaching out of the enzymes from the porous matrix. The free as well as the gel-entrapped concanavalin A-glycoenzyme complexes exhibited a relatively high effectiveness factor, e...

journal_title：Biotechnology and bioengineering

authors： Husain Q,Iqbal J,Saleemuddin M

更新日期：1985-08-01 00:00:00

abstract：:A semiequilibrium model of chromatography has been developed to account for the elution profile of high concentration bands. The use of this model requires the prior determination of the equilibrium isotherms of the studied compounds in the chromatographic phase system. The model is applied here to a study of the isoc...

journal_title：Biotechnology and bioengineering

authors： Katti A,Huang JX,Guiochon G

更新日期：1990-07-01 00:00:00

abstract：:We have identified an error in the published integral form of the modified Michaelis-Menten equation that accounts for endogenous substrate production. The correct solution is presented and the error in both the substrate concentration, S, and the kinetic parameters Vm , Km , and R resulting from the incorrect solutio...

journal_title：Biotechnology and bioengineering

authors： Goudar CT

更新日期：2011-10-01 00:00:00

abstract：:Pronounced spatial nonuniformities in cell density, physiology, and activity frequently arise within densely packed immobilized cell supports. For a more fundamental understanding of immobilized cell phenomena, we have developed high-resolution microfluorimetric procedures to analyze local variations in both immobiliz...

journal_title：Biotechnology and bioengineering

authors： Kuhn RH,Peretti SW,Ollis DF

更新日期：1991-08-05 00:00:00

abstract：:A major challenge when designing cell scaffolds for chondrocyte delivery in vivo is creating scaffolds with sufficient mechanical properties to restore initial function while simultaneously controlling temporal changes in the gel structure to facilitate tissue formation. To address this design challenge, degradable ph...

journal_title：Biotechnology and bioengineering

authors： Bryant SJ,Bender RJ,Durand KL,Anseth KS

更新日期：2004-06-30 00:00:00

abstract：:We have previously reported the development of a novel protein expression system based on Ralstonia eutropha. In this study we report on the influence of gene copynumber on recombinant protein expression in R. eutropha. We compare recombinant gene stability and expression levels of chromosomal integration with a plasm...

journal_title：Biotechnology and bioengineering

authors： Srinivasan S,Barnard GC,Gerngross TU

更新日期：2003-10-05 00:00:00