Production of recombinant proteins using multiple-copy gene integration in high-cell-density fermentations of Ralstonia eutropha.

abstract：:Cellulase (Cellulosin AC-8) was immobilized on poly-L-glutamic acid. This immobilized cellulase (IC) is water soluble in the neutral and alkaline solutions, where IC has the activity, while IC can be made insoluble by lowering the pH so that it can be recovered from the reaction mixture with its activity. The optimum ...

journal_title：Biotechnology and bioengineering

authors： Takeuchi T,Makino K

更新日期：1987-02-01 00:00:00

abstract：:A model that portrays substrate profiles in a steady-state multispecies granular biofilm is developed and coupled with a biofilm detachment model. The model accounts for glucose, propionate, hydrogen, and acetate transformations performed by three bacterial trophic groups: acidogens, syntrophic bacterial consortia, an...

journal_title：Biotechnology and bioengineering

authors： Tartakovsky B,Guiot SR

更新日期：1997-04-20 00:00:00

abstract：:Although several compelling benefits for bioprocess intensification have been reported, the need for a streamlined integration of perfusion cultures with capture chromatography still remains unmet. Here, a robust solution is established by conducting tangential flow filtration-based perfusion with a wide-surface pore ...

journal_title：Biotechnology and bioengineering

authors： Pinto NDS,Brower M

更新日期：2020-07-15 00:00:00

abstract：:Engineered strains of Saccharomyces cerevisiae are used for industrial production of succinic acid. Optimal process conditions for dicarboxylic-acid yield and recovery include slow growth, low pH, and high CO2 . To quantify and understand how these process parameters affect yeast physiology, this study investigates in...

journal_title：Biotechnology and bioengineering

authors： Hakkaart X,Liu Y,Hulst M,El Masoudi A,Peuscher E,Pronk J,van Gulik W,Daran-Lapujade P

更新日期：2020-03-01 00:00:00

abstract：:Recombinant retroviruses are effective vectors able to integrate transgenes into the target cell's genome to achieve longer-term expression. This study investigates the effect of cell lysis products, a common cell culture by-product, on the transduction of suspension cells by gammaretroviral vectors. Cell lysates deri...

journal_title：Biotechnology and bioengineering

authors： Beauchesne PR,Bruce KJ,Bowen BD,Piret JM

更新日期：2010-04-15 00:00:00

abstract：:Generating stable, high-producing cell lines for recombinant protein production requires an understanding of the potential limitations in the cellular machinery for protein expression. In order to increase our understanding of what makes a stable high producer, we have generated a panel of 17 recombinant monoclonal an...

journal_title：Biotechnology and bioengineering

authors： Chusainow J,Yang YS,Yeo JH,Toh PC,Asvadi P,Wong NS,Yap MG

更新日期：2009-03-01 00:00:00

abstract：:The production of beta-galactosidase from the E. coli phoA-lacZ fusion gene was studied to compare the gene expression behavior of two cloning methods: insertion to multicopy plasmids and integration into host cell's chromosome. The chromosome-integrating strain showed more tight control of fusion gene expression leve...

journal_title：Biotechnology and bioengineering

authors： Shin PK,Seo JH

更新日期：1990-12-20 00:00:00

abstract：:Whole cell pyruvate decarboxylase (PDC) from Candida utilis enhanced the enzymatic production of (R)-phenylacetylcarbinol (PAC) in an aqueous/octanol biotransformation compared to the partially purified PDC especially for a lower range of initial activities (0.3-2.5 U/mL). With an initial activity of 1.1 U/mL and at a...

journal_title：Biotechnology and bioengineering

authors： Satianegara G,Rogers PL,Rosche B

更新日期：2006-08-20 00:00:00

abstract：:Immobilization of Streptomyces phaerochromogenes was studied by radiation-induced polymerization of 2-hydroxyethyl methacrylate at low temperatures. Radiation damage of the enzyme could be avoided by choosing irradiation at low temperatures. The enzymatic activity of immobilized cells increased remarkably with a decre...

journal_title：Biotechnology and bioengineering

authors： Kumakura M,Yoshida M,Kaetsu I

更新日期：1979-04-01 00:00:00

abstract：:A two-stage deterministic model of the growth of Saccharomyces cerevisiae is presented. The cell cycle of this organism was used to suggest the basic model structure. The model represents the preparatory processes of substrate uptake and conversion separately from replication and division. The regulation of the fracti...

journal_title：Biotechnology and bioengineering

authors： Bijkerk AH,Hall RJ

更新日期：1977-02-01 00:00:00

abstract：:The bioartificial pancreas encapsulating pancreatic islets in immunoprotective hydrogel is a promising therapy for Type 1 diabetes. As pancreatic islets are highly metabolically active and exquisitely sensitive to hypoxia, maintaining O2 supply after transplantation remains a major challenge. In this study, we address...

journal_title：Biotechnology and bioengineering

authors： Mouré A,Bacou E,Bosch S,Jegou D,Salama A,Riochet D,Gauthier O,Blancho G,Soulillou JP,Poncelet D,Olmos E,Bach JM,Mosser M

更新日期：2019-05-01 00:00:00

abstract：:Microplates with integrated optical oxygen sensors are a new tool to study metabolic rates and enzyme activities. Precise measurements are possible only if oxygen exchange between the sample and the environment is known. In this study we quantify gas exchange in plastic microplates. Dissolved oxygen was detected using...

journal_title：Biotechnology and bioengineering

authors： Arain S,Weiss S,Heinzle E,John GT,Krause C,Klimant I

更新日期：2005-05-05 00:00:00

abstract：:Globally, approximately a billion patients are estimated to suffer from neurological disorders. Although there are many therapeutic candidates for the central nervous system, treatment of brain disorders is restricted by the blood-brain barrier (BBB), which is a highly selective membrane that protects the brain from e...

journal_title：Biotechnology and bioengineering

authors： Kim D,Kwon S

更新日期：2018-10-01 00:00:00

abstract：:Relative levels of many individual proteins in Escherichia coli HB101 strains with 0, 37, 56, and 240 plasmids per chromosome were determined by computer image analysis of two-dimensional gel electrophoresis patterns. The plasmids investigated had very similar sequences except for small domains encoding the repressor ...

journal_title：Biotechnology and bioengineering

authors： Birnbaum S,Bailey JE

更新日期：1991-04-05 00:00:00

abstract：:Lignin, one of the major components of lignocellulosic biomass, plays an inhibitory role on the enzymatic hydrolysis of cellulose. This work examines the role of lignin in pretreated hardwood, where extents of cellulose hydrolysis decrease, rather than increase with increasing severity of liquid hot water pretreatment...

journal_title：Biotechnology and bioengineering

authors： Ko JK,Kim Y,Ximenes E,Ladisch MR

更新日期：2015-02-01 00:00:00

abstract：:The knowledge of critical process-relevant genes can be used for an improved control of bioprocesses. So far bioprocess-relevant marker genes can be analyzed by established expression analysis methods only off-line. In this study, an alternative approach for a potential at-line monitoring of gene expression during bio...

journal_title：Biotechnology and bioengineering

authors： Jürgen B,Barken KB,Tobisch S,Pioch D,Wümpelmann M,Hecker M,Schweder T

更新日期：2005-11-05 00:00:00

abstract：:The Chinese hamster ovary (CHO) cell line producing interferon-gamma (IFN-gamma) exhibits a 2-fold increase in specific productivity when grown at 32 degrees C compared to 37 degrees C. Low temperature also causes growth arrest, meaning that the cell density is significantly lower at 32 degrees C, nutrients are consum...

journal_title：Biotechnology and bioengineering

authors： Fox SR,Patel UA,Yap MG,Wang DI

更新日期：2004-01-20 00:00:00

abstract：:The problems of engineering increased flux in metabolic pathways are analyzed in terms of the understanding provided by metabolic control analysis. Over-expression of a single enzyme is unlikely to be effective unless it is known to have a high flux control coefficient, which can be used as an approximate predictive t...

journal_title：Biotechnology and bioengineering

authors： Fell DA

更新日期：1998-04-05 00:00:00

abstract：:Three-dimensional (3D) tumor models have been established in various microfluidic systems for drug delivery and resistance studies in vitro. However, one of the main drawbacks of these models is non-uniform distribution of cells, leaving regions with very low cell density within the 3D structures. As a result, molecul...

journal_title：Biotechnology and bioengineering

authors： Elliott NT,Yuan F

更新日期：2012-05-01 00:00:00

abstract：:Modern large-scale agricultural practices that incorporate high density farming with subtherapeutic antibiotic dosing are considered a major contributor to the rise of antibiotic-resistant bacterial infections of humans with species of Salmonella being a leading agriculture-based bacterial infection. Microcin J25, a p...

journal_title：Biotechnology and bioengineering

authors： Ritter SC,Yang ML,Kaznessis YN,Hackel BJ

更新日期：2018-10-01 00:00:00

abstract：:Batch hydrolysis kinetics of paper birch (Betula papyrifera) xylan and its associated acetyl groups in dilute sulfuric acid have been measured for acid concentrations of between 0.04 and 0.18M and temperatures of between 100 and 170 degrees C. Only 5% of the cellulose was hydrolyzed for up to 85% xylan removal. Rate d...

journal_title：Biotechnology and bioengineering

authors： Maloney MT,Chapman TW,Baker AJ

更新日期：1985-03-01 00:00:00

abstract：:Neural networks are formed by accurate connectivity of neurons and glial cells in the brain. These networks employ a three-dimensional bio-surface that both assigns precise coordinates to cells during development and facilitates their connectivity and functionality throughout life. Using specific topographic and chemi...

journal_title：Biotechnology and bioengineering

authors： Bani-Yaghoub M,Tremblay R,Voicu R,Mealing G,Monette R,Py C,Faid K,Sikorska M

更新日期：2005-11-05 00:00:00

abstract：:A method for purifying alpha-amylase inhibitor from wheat meal based on immobilized metal affinity with a thermosensitive copolymer is developed. The studies represent the thermoprecipitation properties of the copolymers of N-isopropylacrylamide (NIPAM) with iminodiacetic acid (IDA) and 1-vinylimidazole (VI), respecti...

journal_title：Biotechnology and bioengineering

authors： Kumar A,Galaev IY,Mattiasson B

更新日期：1998-09-20 00:00:00

abstract：:The possibility of following the biomass growth by pressure drop measurement was investigated in an aerated cocurrent upflow fixed-bed bioreactor continuously fed with wastewater containing industrial organic pollutants. The experiments were carried out in a biological filtration oxygenated reactor (Biofor) pilot plan...

journal_title：Biotechnology and bioengineering

authors： Deront M,Samb FM,Adler N,Peringer P

更新日期：1998-10-05 00:00:00

abstract：:In this study, omics-based analysis tools were used to explore the effect of glucose starvation and culture duration on monoclonal antibody (mAb) production in fed-batch CHO cell culture to gain better insight into how these parameters can be controlled to ensure optimal mAb productivity and quality. Titer and N-glyco...

journal_title：Biotechnology and bioengineering

authors： Fan Y,Jimenez Del Val I,Müller C,Lund AM,Sen JW,Rasmussen SK,Kontoravdi C,Baycin-Hizal D,Betenbaugh MJ,Weilguny D,Andersen MR

更新日期：2015-10-01 00:00:00

abstract：:The effect of protein concentration in partitioning in PEG/salt aqueous two-phase systems has been investigated. PEG 4000/phosphate systems in the presence of 0% w/w and 8.8% w/w NaCl have been evaluated using amyloglucosidase, subtilisin, and trypsin inhibitor. Also, a PEG 4000/phosphate system with 3% w/w NaCl was u...

journal_title：Biotechnology and bioengineering

authors： Schmidt AS,Andrews BA,Asenjo JA

更新日期：1996-06-20 00:00:00

abstract：:The influence of fluid-dynamic conditions on the yield of Phaeodactylum tricornutum microalgal cultures was analyzed in two stages: first, the influence of air flow rate; second, the influence of using fluid-moving pumps for recirculating the culture. With respect to the air flow rate, the yield of the cultures increa...

journal_title：Biotechnology and bioengineering

authors： Alías CB,García-Malea López MC,Acién Fernández FG,Ferníndez Sevilla JM,García Sánchez JL,Molina Grima E

更新日期：2004-09-20 00:00:00

abstract：:A respiratory-deficient, mutant of Kluyveromyces fragilis was isolated using a ethidium bromide mutagenesis. It was characterized by a loss of cytochromes a + a3 and by an improvement of its inulinase activity. Under anaerobic conditions this mutant was always better than the wild strain for ethanol production especia...

journal_title：Biotechnology and bioengineering

authors： Guiraud JP,Bourgi J,Stervinou M,Claisse M,Galzy P

更新日期：1987-05-01 00:00:00

abstract：:Overexpression of recombinant proteins in Escherichia coli often leads to a severe growth retardation of the host cells. The phage T7 promoter phi10 in a pET vector was utilized to express human superoxide dismutase. Induction with IPTG lead to an increase in protein content and cell size and a termination of cell div...

journal_title：Biotechnology and bioengineering

authors： Soriano E,Borth N,Katinger H,Mattanovich D

更新日期：2002-10-05 00:00:00

abstract：:In our preceding article, we demonstrated a procedure based upon enzymic flow microcalorimetry using an enzyme thermistor (ET) to characterize the microkinetic properties of an immobilized enzyme (IME) and its further application in the screening of IMEs. To consider the ET method (single ET unit, ET system 1) as stan...

journal_title：Biotechnology and bioengineering

authors： Gemeiner P,Docolomansky P,Nahálka J,Stefuca V,Danielsson B

更新日期：1996-01-05 00:00:00