Abstract:
:A successful procedure for attaching cellulase to 45 microm iron oxide particles with a high-molecular-weight (1000) ligand has been developed. Mass and activity balances were calculated for immobilized enzymes with different loadings. The highest specific activity of the immobilized enzyme was 5.9 mmol glucose/g bound protein/h. The efficacy of retaining enzymatic activity was 128%. The optimum pH was 5.5 compared to 4.0 of the free enzyme. The half-life of the IMC was extended to 272 h compared to 0.77 h of the free enzyme.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Garcia A 3rd,Oh S,Engler CRdoi
10.1002/bit.260330311subject
Has Abstractpub_date
1989-01-15 00:00:00pages
321-6issue
3eissn
0006-3592issn
1097-0290journal_volume
33pub_type
杂志文章abstract::A new ultrafiltration technique based on a multimembrane stack has been developed to fractionate solutes closer in size than conventionally possible. The technique is illustrated here by obtaining a pure protein product from a binary protein mixture. By employing membranes in series without any gaskets or spacers in-b...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260260305
更新日期:1984-03-01 00:00:00
abstract::The secretion of heterologous IgG proteins in the baculovirus-insect cell expression system is accompanied by substantial insoluble immunoglobulin in the infected cells. The accumulation of these insoluble forms suggests a limitation in the processing and secretory pathway of the infected cells. As a result, cytosolic...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10142
更新日期:2002-02-15 00:00:00
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journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280203
更新日期:1986-02-01 00:00:00
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doi:10.1002/bit.21829
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doi:10.1002/bit.26255
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