Abstract:
:In analyzing microbial cellulose utilization, it would be useful to independently measure the mass concentration of cells and cellulase enzymes. Such measurements would allow investigation of the allocation of cellular resources between synthesis of cells and cellulase, in vivo cell- and cellulase-specific cellulose hydrolysis rates, and bioenergetics. Methodological protocols are not established for independent determination of cell and cellulase concentrations for the common case in which a substantial fraction of cellulase is attached to the cell surface. Alternative analytical approaches by which to develop such protocols are examined from the perspective of error minimization. For cell concentration measurement, acceptable accuracy is expected when the concentrations of a cell-specific component (e.g., DNA) is determined or when total protein is determined in conjunction with a measurement specific to cellulase. For cellulase concentration measurement, acceptable accuracy is expected when a measurement specific to cellulase such as ELISA is used. Several analytical approaches are rejected based on large expected errors.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Lynd LR,Zhang Ydoi
10.1002/bit.10142subject
Has Abstractpub_date
2002-02-15 00:00:00pages
467-75issue
4eissn
0006-3592issn
1097-0290pii
10.1002/bit.10142journal_volume
77pub_type
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journal_title:Biotechnology and bioengineering
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