Parallel experimental evolution reveals a novel repressive control of GalP on xylose fermentation in Escherichia coli.

abstract：:Using solid-phase organic synthesis, we have prepared a 432-member (18 x 8 x 3) sample library based on the AG 490 "tyrphostin" template. By utilizing 432 reactors each equipped with a unique radiofrequency memory ID tag, the 432 products could be obtained as discrete entities (i.e., not as mixtures) via 18 + 8 + 3, o...

journal_title：Biotechnology and bioengineering

authors： Shi S,Xiao XY,Czarnik AW

更新日期：1998-01-01 00:00:00

abstract：:A stirred bath technique was developed for determining effective diffusivities in cell matrices. The technique involves cell immobilization in a dilute gel which has negligible effect on solute diffusion. Agar and collagen were tested as immobilizing gels. Agar gel was shown to have minor interactions with the diffusi...

journal_title：Biotechnology and bioengineering

authors： Chresand TJ,Dale BE,Hanson SL,Gillies RJ

更新日期：1988-10-05 00:00:00

abstract：:Malathion is one of the most widely used organophosphorus pesticides in the United States and developing countries. Herein, we enhanced the degradation rate of malathion starting with a phosphotriesterase PoOPHM2 while also considering thermostability. In the first step, iterative saturation mutagenesis at residues li...

journal_title：Biotechnology and bioengineering

authors： Luo XJ,Zhao J,Li CX,Bai YP,Reetz MT,Yu HL,Xu JH

更新日期：2016-11-01 00:00:00

abstract：:The progression of liver disease from normal to cirrhotic state is characterized by modulation of the stiffness of the extracellular matrix (ECM). Mimicking this modulation in vitro scaffold could provide a better insight into hepatic cell behavior. In this study, interpenetrating poly(N-isopropylacrylamide-co-gelatin...

journal_title：Biotechnology and bioengineering

authors： Sarkar J,Kamble SC,Patil R,Kumar A,Gosavi SW

更新日期：2020-02-01 00:00:00

abstract：:Zymomonas mobilis is a very important gram-negative bacterium having a potential application to simultaneous co-production of biofuel and other high value-added products through biorefinery process technology development. Up to now, pLOI193 has been used as the plasmid of choice for Z. mobilis strains. However, its ap...

journal_title：Biotechnology and bioengineering

authors： Dong HW,Bao J,Ryu DD,Zhong JJ

更新日期：2011-07-01 00:00:00

abstract：:Differences in plasmid retention and expression are studied in both suspended and biofilm cultures of Escherichia coli DH5alpha(PMJR1750). An alternative mathematical model is proposed which allows the determination of plasmid loss probability in both suspended batch and continuously fed biofilm cultures. In our exper...

journal_title：Biotechnology and bioengineering

authors： Huang CT,Peretti SW,Bryers JD

更新日期：1993-01-20 00:00:00

abstract：:A cybernetic framework is presented which views microbial response in multiple substrate environments as a judicious investment of cellular resources in synthesizing different key proteins according to an optimal regulatory strategy. A mathematical model is developed within the cybernetic framework for the diauxic gro...

journal_title：Biotechnology and bioengineering

authors： Dhurjati P,Ramkrishna D,Flickinger MC,Tsao GT

更新日期：1985-01-01 00:00:00

abstract：:Catalytic membranes, obtained by immobilizing thermophilic beta-glycosidase onto nylon supports, were used in a nonisothermal bioreactor to study the effect of temperature gradients on the rate of enzyme reaction. Two experimental approaches were carried out to explain the molecular mechanisms by which the temperature...

journal_title：Biotechnology and bioengineering

authors： Febbraio F,Portaccio M,Stellato S,Rossi S,Bencivenga U,Nucci R,Rossi M,Gaeta FS,Mita DG

更新日期：1998-07-05 00:00:00

abstract：:Lipase from Candida cylindracea (CCL) was incorporated into polymerizable positively charged dialkylammonium bromide surfactant vesicles. The enzyme was incorporated by the use of the dehydration-rehydration method or by incubation. In the latter case, trapping efficiencies of up to 100% could be obtained. Activities ...

journal_title：Biotechnology and bioengineering

authors： Mosmuller EW,Franssen MC,Engbersen JF

更新日期：1993-06-20 00:00:00

abstract：:High-level expression of recombinant penicillin acylase (PAC) using the strong trc promoter system in Escherichia coli is frequently limited by the processing and folding of PAC precursors (proPAC) in the periplasm, resulting in physiological stress and inclusion body formation in this compartment. Periplasmic heat-sh...

journal_title：Biotechnology and bioengineering

authors： Wu MS,Pan KL,Chou CP

更新日期：2007-04-01 00:00:00

abstract：:Studies have been conducted in a gas circulation type fermentor in order to characterize the ethanol fermentation of uncooked cassava starch with Rhizopus koji. Results showed that ethanol concentration reached 13-14% (v/v) in 4-day broth, and the maximum productivity of ethanol was 2.3 g ethanol/L broth h. This produ...

journal_title：Biotechnology and bioengineering

authors： Fujio Y,Ogata M,Ueda S

更新日期：1985-08-01 00:00:00

abstract：:An approach is presented for the stable covalent immobilization of proteins with a high retention of biological activity. First, chemical modification studies were used to establish enzyme structural and functional properties relevant to the covalent immobilization of an enzyme to agarose based supports. Heparinase wa...

journal_title：Biotechnology and bioengineering

authors： Leckband D,Langer R

更新日期：1991-02-05 00:00:00

abstract：:Utilization of renewable feedstocks for the production of bio-based chemicals such as d-lactic acid by engineering metabolic pathways in the yeast Saccharomyces cerevisiae has recently become an attractive option. In this study, to realize efficient d-lactic acid production by S. cerevisiae, the expression of 12 glyco...

journal_title：Biotechnology and bioengineering

authors： Yamada R,Wakita K,Mitsui R,Ogino H

更新日期：2017-09-01 00:00:00

abstract：:The use of expanded beds of STREAMLINE ion exchange adsorbents for the direct extraction of an intracellular enzyme glucose-6-phosphate dehydrogenase (G6PDH) from unclarified yeast cell homogenates has been investigated. It has been demonstrated that such crude feedstocks can be applied to the bed without prior clarif...

journal_title：Biotechnology and bioengineering

authors： Chang YK,Chase HA

更新日期：1996-01-20 00:00:00

abstract：:A limited life cycle assessment (LCA) was performed on a combined biological and chemical process for the production of adipic acid, which was compared to the traditional petrochemical process. The LCA comprises the biological conversion of the aromatic feedstocks benzoic acid, impure aromatics, toluene, or phenol fro...

journal_title：Biotechnology and bioengineering

authors： van Duuren JB,Brehmer B,Mars AE,Eggink G,Dos Santos VA,Sanders JP

更新日期：2011-06-01 00:00:00

abstract：:The encapsulin nanocompartment from Rhodococcus erythropolis N771 (Reencapsulin) was expressed and purified in wild-type and C-terminally His-tagged forms. Negative-stained transmission electron microscopy, field-flow fractionation combined with multi-angle light scattering and dynamic light scattering analyses showed...

journal_title：Biotechnology and bioengineering

authors： Tamura A,Fukutani Y,Takami T,Fujii M,Nakaguchi Y,Murakami Y,Noguchi K,Yohda M,Odaka M

更新日期：2015-01-01 00:00:00

abstract：:Cultural conditions optimum for beta-galactosidase production by Saccharomyces anamensis are pH 4.5, temperature 26 +/- 2 degrees C, and 30 h of incubation period. Addition of lactose at 24 h fermentation greatly increase the level of enzyme. Optimum pHl, temperature, pH stability, and thermostability of yeast beta-ga...

journal_title：Biotechnology and bioengineering

authors： Banerjee M,Chakrabarty A,Majumdar SK

更新日期：1982-08-01 00:00:00

abstract：:Trehalase is the enzyme which hydrolyzes the disaccharide trehalose into two alpha-D-glucose molecules. In this article, we present the immobilization of trehalase on aminopropyl glass particles. The enzyme was extracted from Escherichia coli Mph2, a strain harboring the pTRE11 plasmid, which contains the trehalase ge...

journal_title：Biotechnology and bioengineering

authors： Bachinski N,Martins AS,Paschoalin VM,Panek AD,Paiva CL

更新日期：1997-04-05 00:00:00

abstract：:An alpha-galactosidase and a beta-mannanase produced by the hyperthermophilic bacterium, Thermotoga neapolitana 5068 (TN5068), separately and together, were evaluated for their ability to hydrolyze guar gum in relation to viscosity reduction of guar-based hydraulic fracturing fluids used in oil and gas well stimulatio...

journal_title：Biotechnology and bioengineering

authors： McCutchen CM,Duffaud GD,Leduc P,Petersen AR,Tayal A,Khan SA,Kelly RM

更新日期：1996-10-20 00:00:00

abstract：:The feasibility of NO3- removal by the synergistic action of a prevailing denitrifying anoxic methane oxidising (DAMO), and nitrate-reducing and sulfide-oxidising bacterial (NR-SOB) consortium, using CH4 and H2 S from biogas as electron donors in a biotrickling filter was investigated. The influence of NO3- concentrat...

journal_title：Biotechnology and bioengineering

authors： López JC,Porca E,Collins G,Pérez R,Rodríguez-Alija A,Muñoz R,Quijano G

更新日期：2017-03-01 00:00:00

abstract：:The redox control bioreactor (RCB) is a new hollow fiber membrane bioreactor (HFMBR) design in which oxygen and hydrogen gases are provided simultaneously through separate arrays of juxtaposed hollow fiber (HF) membranes. This study applied the RCB for completely autotrophic conversion of ammonia to N(2) through nitri...

journal_title：Biotechnology and bioengineering

authors： Smith DP,Rector T,Reid-Black K,Hummerick M,Strayer R,Birmele M,Roberts MS,Garland JL

更新日期：2008-03-01 00:00:00

abstract：:Enzyme clustering into compact agglomerates could accelerate the processing of intermediates to enhance metabolic pathway flux. However, enzyme clustering is still a challenging task due to the lack of universal assembly strategy applicable to all enzymes. Therefore, we proposed an alternative enzyme assembly strategy...

journal_title：Biotechnology and bioengineering

authors： Lv X,Jin K,Wu Y,Zhang C,Cui S,Zhu X,Li J,Du G,Liu L

更新日期：2020-05-01 00:00:00

abstract：:Aspergillus awamori NRRL 4869 was cultured on the solid substrate, wheat bran, in a modified Rollacell apparatus to produce alpha-galactosidase and invertase. The swivel cap on the elongated bottle permits the introduction of air while the bottle rotates. Parameters of air flow rate (0.05-0.2 liter/kg/min), rpm (0.15-...

journal_title：Biotechnology and bioengineering

authors： Silman RW

更新日期：1980-02-01 00:00:00

abstract：:Hairy roots have the potential to produce a variety of valuable small and large molecules. The mist reactor is a gas phase bioreactor that has shown promise for low-cost culture of hairy roots. Using a newer, disposable culture bag, mist reactor performance was studied with two species, Artemisia annua L. and Arachis ...

journal_title：Biotechnology and bioengineering

authors： Sivakumar G,Liu C,Towler MJ,Weathers PJ

更新日期：2010-12-01 00:00:00

abstract：:Seven different supports were compared in solid-phase S(N)Ar and S(N)2 macrocyclization reactions. Product purities were assayed for a relatively facile ring-closure process to give products 1 and 3. Some less-facile ring-closure reactions give the undesired dimeric macrocyclization by-products 2; some of these more-d...

journal_title：Biotechnology and bioengineering

authors： Feng Y,Burgess K

更新日期：2000-01-01 00:00:00

abstract：:Thermodynamic feasibility analysis (TFA) has been used as a tool capable of providing additional constraints to the mass balance-based methods of analysis of metabolic networks (e.g., flux balance analysis). Several publications have recently appeared in which TFA of different metabolic pathways from relatively simple...

journal_title：Biotechnology and bioengineering

authors： Vojinović V,von Stockar U

更新日期：2009-07-01 00:00:00

abstract：:Understanding the regulatory networks which control specific macrophage phenotypes is essential in identifying novel targets to correct macrophage mediated clinical disorders, often accompanied by inflammatory events. Since mesenchymal stromal cells (MSCs) have been shown to play key roles in regulating immune functio...

journal_title：Biotechnology and bioengineering

authors： Barminko JA,Nativ NI,Schloss R,Yarmush ML

更新日期：2014-11-01 00:00:00

abstract：:The development of autoantibodies against factor VIII represents one of the major complications in the treatment of hemophilia A patients. We have employed a novel library system to obtain peptides that specifically neutralize the interaction between factor VIII and these inhibitors. The random peptides are presented ...

journal_title：Biotechnology and bioengineering

authors： Mersich C,Billes W,Pabinger I,Jungbauer A

更新日期：2007-09-01 00:00:00

abstract：:Reducing viscosities of concentrated solutions of therapeutic proteins is important for their subcutaneous and intravenous delivery. Although inorganic salts and optimizing the pH were previously reported to dramatically lower the viscosity of a monoclonal antibody solution, herein we have determined these effects not...

journal_title：Biotechnology and bioengineering

authors： Du W,Klibanov AM

更新日期：2011-03-01 00:00:00

abstract：:Genetically-engineered cells offer a solution to the cell availability problem in tissue engineering a pancreatic substitute for the treatment of insulin-dependent diabetes. These cells can be non-beta cells, such as hepatocytes or myoblasts, retrieved as a biopsy from the same patient and genetically engineered to se...

journal_title：Biotechnology and bioengineering

authors： Cheng SY,Constantinidis I,Sambanis A

更新日期：2006-04-20 00:00:00