Design and construction of improved new vectors for Zymomonas mobilis recombinants.


:Zymomonas mobilis is a very important gram-negative bacterium having a potential application to simultaneous co-production of biofuel and other high value-added products through biorefinery process technology development. Up to now, pLOI193 has been used as the plasmid of choice for Z. mobilis strains. However, its application has been limited due to its relatively low transformation efficiency, a large plasmid size (13.4 kb), and limited choice of cloning sites for gene manipulations. Some of these limitations can be overcome by the newly designed and constructed plasmid pHW20a, which provides significantly higher transformation efficiency (about two orders of magnitude greater), better stability (for at least 120 generation times), and an ease of gene manipulations. The pHW20a contains three complete cis-acting genes (repA, repB, and repC) encoding the Rep proteins for primosome formation. It has the origin of replication (oriV) to ensure replication in gram-negative bacteria, two mob genes that enhances transformation efficiency, a screening marker (lacZα), expanded multiple cloning sites (MCS) that enables easy gene manipulation, and the tetracycline resistance gene (tc(r) ). The utility of screening marker, lacZα with MCS, was confirmed by the blue-white screening test. Several examples of applications of gene expression in Z. mobilis ZM4 have been demonstrated in this article by using several new pHW20a-derived plasmids and expressing the homologous genes (gfo and ppc) and the heterologous genes (bglA, mdh, and fdh1). The results show that pHW20a is a very useful new vector for construction of new Z. mobilis recombinant strains that will enable simultaneous co-production of biofuel and high value added products.


Biotechnol Bioeng


Dong HW,Bao J,Ryu DD,Zhong JJ




Has Abstract


2011-07-01 00:00:00












  • Cell-surface exposure of a hybrid 3-cohesin scaffoldin allowing the functionalization of Escherichia coli envelope.

    abstract::Cellulosomes are large plant cell wall degrading complexes secreted by some anaerobic bacteria. They are typically composed of a major scaffolding protein containing multiple receptors called cohesins, which tightly anchor a small complementary module termed dockerin harbored by the cellulosomal enzymes. In the presen...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Vita N,Borne R,Fierobe HP

    更新日期:2020-03-01 00:00:00

  • A Kalman filter algorithm and monitoring apparatus for at-line control of fractional protein precipitation.

    abstract::Downstream processing operations are often carried out blind in the process timescale since product monitoring on-line is not common. Knowledge of the location and concentration of the product and key contaminants is complementary to other process information for process development and, if available on-line in conjun...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Holwill IJ,Chard SJ,Flanagan MT,Hoare M

    更新日期:1997-01-05 00:00:00

  • Enzymatic synthesis of unsaturated fatty acid glucoside esters for dermo-cosmetic applications.

    abstract::Unsaturated fatty acid alpha-butylglucoside esters were prepared by enzymatic esterification of alpha-butylglucoside in nonaqueous media. Conditions were firstly optimized using oleic acid as acyl group. Synthesis was possible in several solvents but the presence of water co-product in the medium limited the reaction ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Bousquet MP,Willemot RM,Monsan P,Boures E

    更新日期:1999-06-20 00:00:00

  • Mechanistic insights into the liquefaction stage of enzyme-mediated biomass deconstruction.

    abstract::Effective enzyme-mediated viscosity reduction, disaggregation, or "liquefaction," is required to overcome the rheological challenges resulting from the fibrous, hygroscopic nature of lignocellulosic biomass, particularly at the high solids loadings that will be required for an economically viable process. However, the...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: van der Zwan T,Hu J,Saddler JN

    更新日期:2017-11-01 00:00:00

  • Model of oxygen transport limitations in hollow fiber bioreactors.

    abstract::Axial and radial oxygen depletion are believed to be critical scale-limiting factors in the design of cell culture hollow fiber bioreactors. A mathematical analysis of oxygen depletion has been performed in order to develop effectiveness factor plots to aid in the scaling of hollow fiber bioreactors with cells immobil...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Piret JM,Cooney CL

    更新日期:1991-01-05 00:00:00

  • Electrochemical regulation of the end-product profile in Propionibacterium freudenreichii ET-3 with an endogenous mediator.

    abstract::The end-product profile of the glucose fermentation by Propionibacterium freudenreichii ET-3 changed on an electrochemical treatment, in which the culture vessel was filled with a carbon felt anode. Acetate and propionate were produced as final end products in a molar ratio of 2:3 without any electrochemical treatment...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Wang YF,Masuda M,Tsujimura S,Kano K

    更新日期:2008-10-15 00:00:00

  • Mechanistic modeling of enzymatic hydrolysis of cellulose integrating substrate morphology and cocktail composition.

    abstract::A mechanistic model of enzymatic hydrolysis taking into account the morphology of the cellulosic particles and its evolution with time was developed. The individual behavior of the main enzymes involved in the reaction (cellobiohydrolases, endoglucanases, and β-glucosidases), as well as synergy effects, were also incl...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Huron M,Hudebine D,Lopes Ferreira N,Lachenal D

    更新日期:2016-05-01 00:00:00

  • Segmental bone replacement via patient-specific, three-dimensional printed bioresorbable graft substitutes and their use as templates for the culture of mesenchymal stem cells under mechanical stimulation at various frequencies.

    abstract::The treatment of large segmental bone defects remains a challenge as infection, delayed union, and nonunion are common postoperative complications. A three-dimensional printed bioresorbable and physiologically load-sustaining graft substitute was developed to mimic native bone tissue for segmental bone repair. Fabrica...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Chung R,Kalyon DM,Yu X,Valdevit A

    更新日期:2018-09-01 00:00:00

  • Tissue transglutaminase_variant 2-transduced mesenchymal stem cells and their chondrogenic potential.

    abstract::As cartilage is incapable of self-healing upon severe degeneration because of the lack of blood vessels, cartilage tissue engineering is gaining importance in the treatment of cartilage defects. This study was designed to improve cartilage tissue regeneration by expressing tissue transglutaminase variant 2 (TGM2_v2) i...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Calikoglu Koyuncu AC,Nayman AH,Telci D,Torun Kose G

    更新日期:2020-06-01 00:00:00

  • The role of pH on the resistance of resting- and active anammox bacteria to NO2- inhibition.

    abstract::The anaerobic oxidation of ammonium (anammox) uses nitrite as terminal electron acceptor. The nitrite can cause inhibition to the bacteria that catalyze the anammox reaction. The literature shows a great divergence on the levels of NO2 (-) causing inhibition. Moreover, the conditions influencing the resistance of anam...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Carvajal-Arroyo JM,Puyol D,Li G,Sierra-Álvarez R,Field JA

    更新日期:2014-10-01 00:00:00

  • Development of liver microtissues with functional biliary ductular network.

    abstract::Liver tissue engineering aims to create transplantable liver grafts that can serve as substitutes for donor's livers. One major challenge in creating a fully functional liver tissue has been to recreate the biliary drainage in an engineered liver construct through integration of bile canaliculi (BC) with the biliary d...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Hafiz EOA,Bulutoglu B,Mansy SS,Chen Y,Abu-Taleb H,Soliman SAM,El-Hindawi AAF,Yarmush ML,Uygun BE

    更新日期:2021-01-01 00:00:00

  • Detachment of biomass from suspended nongrowing spherical biofilms in airlift reactors.

    abstract::In three-phase internal loop airlift reactors, the detachment of biomass from suspended biofilm pellets in the presence of bare carrier particles was investigated under nongrowth conditions. The detachment rate was dominated by collisions between bare carrier particles and biofilm pellets. The concentration of bare ca...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Gjaltema A,Tijhuis L,van Loosdrecht MC,Heijnen JJ

    更新日期:1995-05-05 00:00:00

  • Microcalorimetric study of cellulose degradation by Cellulomonas uda ATCC 21399.

    abstract::A newly designed batch calorimeter was used to investigate the degradability of some celluloses having varying degrees of crystallinity. The PTC of an aerobic culture of Cellulomonas uda ATCC 21399 obtained revealed a diauxic growth which is attributed to the presence of hemicellulose contaminating Avicel and MN300 ce...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Dermoun Z,Belaich JP

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  • A tightly regulated inducible expression system utilizing the fim inversion recombination switch.

    abstract::The fim inversion system of Escherichia coli (E. coli) can behave as a unidirectional switch in an efficient manner. We have developed a new expression system for E. coli, comprising the arabinose-inducible fimE gene and the fim invertible DNA segment containing a constitutively active promoter. In this system, the ta...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Ham TS,Lee SK,Keasling JD,Arkin AP

    更新日期:2006-05-05 00:00:00

  • Hybridoma perfusion systems: a comparison study.

    abstract::The efficiency of lgM production by hybridoma cells (1) cultured in suspension; (2) entrapped in alginate beads; or (3) packed in hollowfiber cartridge bioreactors, were compared in long-term perfusion cultures. The results showed that steady-state cell concentration and antibody production, per liter of perfused medi...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: de la Broise D,Noiseux M,Massie B,Lemieux R

    更新日期:1992-06-05 00:00:00

  • A comparison of mathematical model predictions to experimental measurements for growth and recombinant protein production in induced cultures of Escherichia coli.

    abstract::Recombinant cell growth and protein synthesis by a recombinant Escherichia coli under various inducing conditions are compared to the predictions of a mathematical model. The mathematical model used was a combination of two literature models: (1) an empirical kinetic model for recombinant growth and product formation ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Betenbaugh MJ,Dhurjati P

    更新日期:1990-06-20 00:00:00

  • Monovalent cations and their influence on activated sludge floc chemistry, structure, and physical characteristics.

    abstract::Multivalent cations have been known to be important components of activated sludge floc structure due to their bridging ability of the negatively charged sites on the biopolymer network. Recently in batch systems it was found that excess concentration of monovalent cations led to the deterioration in settleability, de...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kara F,Gurakan GC,Sanin FD

    更新日期:2008-06-01 00:00:00

  • Modeling brewers' yeast flocculation

    abstract::Flocculation of yeast cells occurs during the fermentation of beer. Partway through the fermentation the cells become flocculent and start to form flocs. If the environmental conditions, such as medium composition and fluid velocities in the tank, are optimal, the flocs will grow in size large enough to settle. After ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: van Hamersveld EH,van der Lans RG,Caulet PJ,Luyben KC

    更新日期:1998-02-05 00:00:00

  • Biomass growth monitoring using pressure drop in a cocurrent biofilter

    abstract::The possibility of following the biomass growth by pressure drop measurement was investigated in an aerated cocurrent upflow fixed-bed bioreactor continuously fed with wastewater containing industrial organic pollutants. The experiments were carried out in a biological filtration oxygenated reactor (Biofor) pilot plan...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Deront M,Samb FM,Adler N,Peringer P

    更新日期:1998-10-05 00:00:00

  • Infrared picosecond laser for perforation of single plant cells.

    abstract::The functional analysis of plant cells at the cellular and subcellular levels requires novel technologies for the directed manipulation of individual cells. In this report, we demonstrate the use of an infrared (1,064 nm) picosecond laser for the perforation of tobacco cell protoplasts. A single pulse was sufficient t...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Schinkel H,Jacobs P,Schillberg S,Wehner M

    更新日期:2008-01-01 00:00:00

  • Small-scale and automatable high-throughput compositional analysis of biomass.

    abstract::Conventional wet chemistry methods to determine biomass composition are labor- and time-intensive and require larger amounts of biomass (300 mg) than is often available. To overcome these limitations and to support a high-throughput pretreatment and hydrolysis (HTPH) screening system, this article reports on the devel...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: DeMartini JD,Studer MH,Wyman CE

    更新日期:2011-02-01 00:00:00

  • Insect larval expression process is optimized by generating fusions with green fluorescent protein.

    abstract::The insect larvae/baculovirus protein production process was dramatically simplified by expressing fusion proteins containing green fluorescent protein (GFP) and the product-of-interest. In this case, human interleukin-2 (hIL-2) and chloramphenicol acetyl-transferase (CAT) were model products. Specifically, our fusion...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Cha HJ,Dalal NG,Pham MQ,Vakharia VN,Rao G,Bentley WE

    更新日期:1999-11-05 00:00:00

  • High-cell-density fed-batch cultivation of the docosahexaenoic acid producing marine alga Crypthecodinium cohnii.

    abstract::The heterotrophic marine alga Crypthecodinium cohnii is known to produce docosahexaenoic acid (DHA), a polyunsaturated fatty acid with food and pharmaceutical applications, during batch cultivation on complex media containing sea salt, yeast extract, and glucose. In the present study, fed-batch cultivation was studied...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: De Swaaf ME,Sijtsma L,Pronk JT

    更新日期:2003-03-20 00:00:00

  • Methodology for real-time, multianalyte monitoring of fermentations using an in-situ mid-infrared sensor.

    abstract::An in-situ, mid-infrared sensor was used to monitor the major analyte concentrations involved in the cultivation of Gluconacetobacter xylinus and the production of gluconacetan, a food-grade exopolysaccharide. To predict the analyte concentrations, three different sets of standard spectra were used to develop calibrat...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Kornmann H,Rhiel M,Cannizzaro C,Marison I,von Stockar U

    更新日期:2003-06-20 00:00:00

  • Invertase immobilization via its carbohydrate moiety.

    abstract::After periodate oxidation of its glycosidic component, invertase was covalently bound onto three types of modified solid supports: glycidyl methacrylate, styrene-divinylbenzene copolymers, and bead cellulose. Direct reaction of the invertase aldehyde groups that were formed with amino groups of the support and use of ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Marek M,Valentová O,Kás J

    更新日期:1984-10-01 00:00:00

  • Plasmid presence changes the relative levels of many host cell proteins and ribosome components in recombinant Escherichia coli.

    abstract::Relative levels of many individual proteins in Escherichia coli HB101 strains with 0, 37, 56, and 240 plasmids per chromosome were determined by computer image analysis of two-dimensional gel electrophoresis patterns. The plasmids investigated had very similar sequences except for small domains encoding the repressor ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Birnbaum S,Bailey JE

    更新日期:1991-04-05 00:00:00

  • Survival of aging CD264+ and CD264- populations of human bone marrow mesenchymal stem cells is independent of colony-forming efficiency.

    abstract::In vivo mesenchymal stem cell (MSC) survival is relevant to therapeutic applications requiring engraftment and potentially to nonengraftment applications as well. MSCs are a mixture of progenitors at different stages of cellular aging, but the contribution of this heterogeneity to the survival of MSC implants is unkno...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Madsen SD,Jones SH,Tucker HA,Giler MK,Muller DC,Discher CT,Russell KC,Dobek GL,Sammarco MC,Bunnell BA,O'Connor KC

    更新日期:2020-01-01 00:00:00

  • Fluorescent detection of cyanobacterial DNA using bacterial magnetic particles on a MAG-microarray.

    abstract::Bacterial magnetic particles (BMPs) were used for the identification of cyanobacterial DNA. Genus-specific oligonucleotide probes for the detection of Anabaena spp., Microcystis spp., Nostoc spp., Oscillatoria spp., and Synechococcus spp. were designed from the variable region of the cyanobacterial 16S rDNA of 148 str...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Matsunaga T,Nakayama H,Okochi M,Takeyama H

    更新日期:2001-06-05 00:00:00

  • Cellulase production by trichoderma harzianum in static and mixed solid-state fermentation reactors under nonaseptic conditions.

    abstract::Cellulase production from lignocellulosic materials was studied in solid-state cultivation by both static and mixed techniques under nonaseptic conditions. The effects of fermentation conditions, such as moisture content, pH, temperature, and aeration, on cellulase production by Trichoderma harzianum using a mixture o...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Deschamps F,Giuliano C,Asther M,Huet MC,Roussos S

    更新日期:1985-09-01 00:00:00

  • Co-opting biology to deliver drugs.

    abstract::The goal of drug delivery is to improve the safety and therapeutic efficacy of drugs. This review focuses on delivery platforms that are either derived from endogenous pathways, long-circulating biomolecules and cells or that piggyback onto long-circulating biomolecules and cells. The first class of such platforms is ...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章,评审


    authors: Yousefpour P,Chilkoti A

    更新日期:2014-09-01 00:00:00