Peptide synthesis with a proteinase from the extremely thermophilic organism Thermus Rt41A.

abstract：:In recent years there has been a considerable interest in developing membrane chromatography systems that function as a short, wide chromatographic column in which the adsorptive packing consists of one or more microporous membranes. This study reports the use of new adsorber membranes prepared by the incorporation of...

journal_title：Biotechnology and bioengineering

authors： Avramescu ME,Borneman Z,Wessling M

更新日期：2003-12-05 00:00:00

abstract：:Rhodococcus rhodochrous IGTS8 was previously isolated because of its ability to use coal as its sole source of sulfur for growth. Subsequent growth studies have revealed that IGTS8 is capable of using a variety of organosulfur compounds as sources of sulfur but not carbon. In this article, the ability of IGTS8 to sele...

journal_title：Biotechnology and bioengineering

authors： Kilbane JJ 2nd,Jackowski K

更新日期：1992-11-01 00:00:00

abstract：:Gene expression microarrays are a relatively new technology, dating back just a few years, yet they have already become a very widely used tool in biology, and have evolved to a wide range of applications well beyond their original design intent. However, while the use of microarrays has expanded, and the issues of pe...

journal_title：Biotechnology and bioengineering

authors： Grant GR,Manduchi E,Pizarro A,Stoeckert CJ Jr

更新日期：2003-12-30 00:00:00

abstract：:The ability to capture cell-free DNA from the gastrointestinal tract, in a minimally invasive manner, could enhance our ability to diagnose gastrointestinal disease, or gain a better understanding of the spatial mapping of the intestinal microbiota. We, therefore, sought to identify a class of capture agents that coul...

journal_title：Biotechnology and bioengineering

authors： Hilmer AJ,Jeffrey RB,Park WG,Khosla C

更新日期：2017-04-01 00:00:00

abstract：:The analysis of a continuous, aerobic, fixed-film bioreactor is performed by simulating the behavior of penicillin production in a three-phase fluidized bed. Rigorous mathematical models are developed for a fluidized-bed fermentor in which bioparticles are fluidized by the liquid medium and air. The steady-state perfo...

journal_title：Biotechnology and bioengineering

authors： Park Y,Davis ME,Wallis DA

更新日期：1984-05-01 00:00:00

abstract：:At the most fundamental level, saccharification occurs when cell wall degrading enzymes (CWDEs) diffuse, bind to and react on readily accessible cellulose fibrils. Thus, the study of the diffusive behavior of solutes into and out of cellulosic substrates is important for understanding how biomass pore size distributio...

journal_title：Biotechnology and bioengineering

authors： Yang D,Moran-Mirabal JM,Parlange JY,Walker LP

更新日期：2013-11-01 00:00:00

abstract：:The adsorption of Thiobacillus ferrooxidans to coal surfaces has been studied. Adsorption experiments were conducted on coal samples from eight different Eastern coal fields. In all cases the adsorption process was at least 90% complete within the first two minutes following inoculation. The results of these experimen...

journal_title：Biotechnology and bioengineering

authors： Bagdigian RM,Myerson AS

更新日期：1986-04-01 00:00:00

abstract：:Enzymatic hydrolysis of cellulose for sugar production offers advantages of higher conversion, minimal by-product formation, low energy requirements, and mild operating conditions over other chemical conversions. The development of a kinetic model, based on observable, macroscopic properties of the overall system, is ...

journal_title：Biotechnology and bioengineering

authors： Wald S,Wilke CR,Blanch HW

更新日期：1984-03-01 00:00:00

abstract：:The use of microalgal biomass for metal pollutant bioremediation might be improved by genetic engineering to modify the selectivity or capacity of metal biosorption. A plant cadmium (Cd) and zinc (Zn) transporter (AtHMA4) was used as a transgene to increase the ability of Chlamydomonas reinhardtii to tolerate 0.2 mM C...

journal_title：Biotechnology and bioengineering

authors： Ibuot A,Webster RE,Williams LE,Pittman JK

更新日期：2020-10-01 00:00:00

abstract：:We have previously reported the development of a novel protein expression system based on Ralstonia eutropha. In this study we report on the influence of gene copynumber on recombinant protein expression in R. eutropha. We compare recombinant gene stability and expression levels of chromosomal integration with a plasm...

journal_title：Biotechnology and bioengineering

authors： Srinivasan S,Barnard GC,Gerngross TU

更新日期：2003-10-05 00:00:00

abstract：:Flow visualization using fluorescent microparticles and cell viability investigations are carried out to examine the mechanisms by which cells are seeded into scaffolds driven by surface acoustic waves. The former consists of observing both the external flow prior to the entry of the suspension into the scaffold and t...

journal_title：Biotechnology and bioengineering

authors： Bok M,Li H,Yeo LY,Friend JR

更新日期：2009-06-01 00:00:00

abstract：:A semiequilibrium model of chromatography has been developed to account for the elution profile of high concentration bands. The use of this model requires the prior determination of the equilibrium isotherms of the studied compounds in the chromatographic phase system. The model is applied here to a study of the isoc...

journal_title：Biotechnology and bioengineering

authors： Katti A,Huang JX,Guiochon G

更新日期：1990-07-01 00:00:00

abstract：:Cellulase production from lignocellulosic materials was studied in solid-state cultivation by both static and mixed techniques under nonaseptic conditions. The effects of fermentation conditions, such as moisture content, pH, temperature, and aeration, on cellulase production by Trichoderma harzianum using a mixture o...

journal_title：Biotechnology and bioengineering

authors： Deschamps F,Giuliano C,Asther M,Huet MC,Roussos S

更新日期：1985-09-01 00:00:00

abstract：:Chondral and osteochondral lesions represent one of the most challenging problems in the orthopedic field, as these types of injuries lead to disability and worsened quality of life for patients and have an economic impact on the healthcare system. The aim of this in vivo study was to develop a new tissue engineering ...

journal_title：Biotechnology and bioengineering

authors： Tschon M,Brogini S,Parrilli A,Bertoldi S,Silini A,Parolini O,Faré S,Martini L,Veronesi F,Fini M,Giavaresi G

更新日期：2021-01-01 00:00:00

abstract：:Flocculation of yeast cells occurs during the fermentation of beer. Partway through the fermentation the cells become flocculent and start to form flocs. If the environmental conditions, such as medium composition and fluid velocities in the tank, are optimal, the flocs will grow in size large enough to settle. After ...

journal_title：Biotechnology and bioengineering

authors： van Hamersveld EH,van der Lans RG,Caulet PJ,Luyben KC

更新日期：1998-02-05 00:00:00

abstract：:Increased stability at alkaline pH should be a valuable attribute for the utilization of penicillin acylase in bioreactors employed to convert penicillins into 6-aminopenicillanic acid, a precursor of semisynthetic penicillins. In these systems, base is added for pH control, which results in local alkaline conditions ...

journal_title：Biotechnology and bioengineering

authors： Del Río G,Rodríguez ME,Munguía ME,López-Munguí A,Soberón X

更新日期：1995-10-20 00:00:00

abstract：:The production of dry-grind corn ethanol results in the generation of intermediate products, thin and whole stillage, which require energy-intensive downstream processing for conversion into commercial animal feed products. Hydrothermal carbonization of thin and whole stillage coupled with anaerobic digestion was inve...

journal_title：Biotechnology and bioengineering

authors： Wood BM,Jader LR,Schendel FJ,Hahn NJ,Valentas KJ,McNamara PJ,Novak PM,Heilmann SM

更新日期：2013-10-01 00:00:00

abstract：:A mechanistic model of enzymatic hydrolysis taking into account the morphology of the cellulosic particles and its evolution with time was developed. The individual behavior of the main enzymes involved in the reaction (cellobiohydrolases, endoglucanases, and β-glucosidases), as well as synergy effects, were also incl...

journal_title：Biotechnology and bioengineering

authors： Huron M,Hudebine D,Lopes Ferreira N,Lachenal D

更新日期：2016-05-01 00:00:00

abstract：:Glycosylation is a key critical quality attribute for monoclonal antibodies and other recombinant proteins because of its impact on effector mechanisms and half-life. In this study, a variety of compounds were evaluated for their ability to modulate glycosylation profiles of recombinant monoclonal antibodies produced ...

journal_title：Biotechnology and bioengineering

authors： Ehret J,Zimmermann M,Eichhorn T,Zimmer A

更新日期：2019-04-01 00:00:00

abstract：:A simple approach was developed to determine the half-saturation coefficient for dissolved oxygen (K(DO)) for three bacteria by maintaining a constant oxygen concentration in continuous culture, and employing a dynamic method to obtain the specific growth rate (mu) for each species. Measurement of mu at selected disso...

journal_title：Biotechnology and bioengineering

authors： Hao OJ,Richard MG,Jenkins D,Blanch HW

更新日期：1983-02-01 00:00:00

abstract：:Autotrophic or mixotrophic use of one-carbon (C1) compounds is gaining importance for sustainable bioproduction. In an effort to integrate the reductive glycine pathway (rGP) as a highly promising pathway for the assimilation of CO2 and formate, genes coding for glycine synthase system from Gottschalkia acidurici were...

journal_title：Biotechnology and bioengineering

authors： Hong Y,Arbter P,Wang W,Rojas LN,Zeng AP

更新日期：2020-12-17 00:00:00

abstract：:It is a common practice in biotherapeutic manufacturing to define a fixed-volume feed strategy for nutrient feeds, based on historical cell demand. However, once the feed volumes are defined, they are inflexible to batch-to-batch variations in cell growth and physiology and can lead to inconsistent productivity and pr...

journal_title：Biotechnology and bioengineering

authors： Zhang A,Tsang VL,Moore B,Shen V,Huang YM,Kshirsagar R,Ryll T

更新日期：2015-12-01 00:00:00

abstract：:After injury to the central nervous system, a glial scar develops that physically and biochemically inhibits axon growth. In the scar, activated astrocytes secrete inhibitory extracellular matrix, of which chondroitin sulfate proteoglycans (CSPGs) are considered the major inhibitory component. An inhibitory interface ...

journal_title：Biotechnology and bioengineering

authors： Tuinstra HM,Ducommun MM,Briley WE,Shea LD

更新日期：2013-03-01 00:00:00

abstract：:We describe a novel procedure to estimate the net growth rate of biofilms on multiple substrates. The approach is based on diffusion-reaction mass balances for chemical species in a continuum biofilm model with reaction kinetics corresponding to a Double-Monod expression. This analytical model considers a heterogeneou...

journal_title：Biotechnology and bioengineering

authors： Gonzo EE,Wuertz S,Rajal VB

更新日期：2014-11-01 00:00:00

abstract：:While elastin-like polypeptides and peptides (ELPs) have been used for various stimulus-responsive applications, details of their switching remain unclear. We therefore constructed a novel series of filamentous phage particles displaying a high surface density of short ELPs. The surface display of ELPs did not disrupt...

journal_title：Biotechnology and bioengineering

authors： Hathorne AP,Bermudez H

更新日期：2013-07-01 00:00:00

abstract：:The specific uptake rate of phenol by washed cells of Pseudomonas putida grown on phenol in steady-state continuous culture at various dilution rates was studied. The Monod-Haldane-type equation was applied to fit the data and the best kinetic parameters were determined by nonlinear least-squares techniques. The value...

journal_title：Biotechnology and bioengineering

authors： Sokol W

更新日期：1987-12-05 00:00:00

abstract：:Proper analysis of label distribution in metabolic pathway intermediates is critical for correct interpretation of experimental data and strategic experimental design. While, for example, 13C nuclear magnetic resonance (NMR) spectroscopy is usually limited to the measurement of degrees of 13C enrichment, more informat...

journal_title：Biotechnology and bioengineering

authors： Klapa MI,Park SM,Sinskey AJ,Stephanopoulos G

更新日期：1999-02-01 00:00:00

abstract：:Cellulase (Cellulosin AC-8) was immobilized on poly-L-glutamic acid. This immobilized cellulase (IC) is water soluble in the neutral and alkaline solutions, where IC has the activity, while IC can be made insoluble by lowering the pH so that it can be recovered from the reaction mixture with its activity. The optimum ...

journal_title：Biotechnology and bioengineering

authors： Takeuchi T,Makino K

更新日期：1987-02-01 00:00:00

abstract：:Hydrophobins are small highly surface-active fungal proteins with potential as biosurfactants in a wide array of applications. However, practical implementation of hydrophobins at large scale has been hindered by low recombinant yields. In this study, the effects of increasing hydrophobin gene copy number and overexpr...

journal_title：Biotechnology and bioengineering

authors： Sallada ND,Harkins LE,Berger BW

更新日期：2019-08-01 00:00:00

abstract：:This paper describes batch and semicontinuous acetic acid fermentations for wine vinegar production carried out with Acetobacter pasteurianus, and an industrial strain using a noncommercial 100-L bubble column reactor equipped with a novel type of gas-liquid dynamic sparger. Results showed acetification rates with thi...

journal_title：Biotechnology and bioengineering

authors： Fregapane G,Rubio-Fernandez H,Nieto J,Salvador MD

更新日期：1999-04-20 00:00:00