Gene delivery to overcome astrocyte inhibition of axonal growth: an in vitro model of the glial scar.

abstract：:Extraction of intracellular protein from Escherichia coli is traditionally achieved by mechanical disruption. A chemical treatment that destroys the integrity of the bacterial cell wall and could provide an alternative technique is examined in this study. Treatment with a combination of the chelating agent ethylenedia...

journal_title：Biotechnology and bioengineering

authors： Falconer RJ,O'Neill BK,Middelberg AP

更新日期：1997-03-05 00:00:00

abstract：:A complete, integrated process for the production of an innovative formulation of penicillin G acylase from Providencia rettgeri(rPAC(P.rett))of industrial applicability is reported. In order to improve the yield of rPAC, the clone LN5.5, carrying four copies of pac gene integrated into the genome of Pichia pastoris, ...

journal_title：Biotechnology and bioengineering

authors： Senerovic L,Stankovic N,Spizzo P,Basso A,Gardossi L,Vasiljevic B,Ljubijankic G,Tisminetzky S,Degrassi G

更新日期：2006-02-05 00:00:00

abstract：:Effectively releasing the locked polysaccharides from recalcitrant lignocellulose to fermentable sugars is among the greatest technical and economic barriers to the realization of lignocellulose biorefineries because leading lignocellulose pre-treatment technologies suffer from low sugar yields, and/or severe reaction...

journal_title：Biotechnology and bioengineering

authors： Zhang YH,Ding SY,Mielenz JR,Cui JB,Elander RT,Laser M,Himmel ME,McMillan JR,Lynd LR

更新日期：2007-06-01 00:00:00

abstract：:The microscopic morphology of Trichoderma reesei QM 9414, growing in submerged culture, was studied by image analysis. The morphology was characterized by the total hyphal length, the total number of tips, the number of actively growing tips, and the length of the main hypha. To describe the growth of a single myceliu...

journal_title：Biotechnology and bioengineering

authors： Lejeune R,Nielsen J,Baron GV

更新日期：1995-09-05 00:00:00

abstract：:The use of expanded beds of STREAMLINE ion exchange adsorbents for the direct extraction of an intracellular enzyme glucose-6-phosphate dehydrogenase (G6PDH) from unclarified yeast cell homogenates has been investigated. It has been demonstrated that such crude feedstocks can be applied to the bed without prior clarif...

journal_title：Biotechnology and bioengineering

authors： Chang YK,Chase HA

更新日期：1996-01-20 00:00:00

abstract：:Buddleja davidii is a unique biomass that has many attractive agroenergy features, especially its wide range of growth habitat. The anatomical characteristics of B. davidii were investigated before and after ethanol organosolv pretreatment (one of the leading pretreatment technologies) in order to further understand t...

journal_title：Biotechnology and bioengineering

authors： Hallac BB,Ray M,Murphy RJ,Ragauskas AJ

更新日期：2010-12-01 00:00:00

abstract：:Model experiments were performed with starved yeast (Saccharomyces cerevisiae) cultures in a batch reactor in order to develop a better understanding of NAD(P)H and flavin culture fluorescence. Fluorescence was monitored during aerobic-anaerobic-aerobic transitions and ethanol and glucose substrate addition experiment...

journal_title：Biotechnology and bioengineering

authors： Siano SA,Mutharasan R

更新日期：1989-08-20 00:00:00

abstract：:Connexin-43 (Cx43) containing giant liposomes (GL) were prepared by a baculovirus expression-liposome fusion method. Recombinant budded viruses expressing Cx43 were prepared and then fused with GLs containing DOPG/DOPC at pH 4.5. Connexon formation on the GL membrane was observed by transmission electron microscope. H...

journal_title：Biotechnology and bioengineering

authors： Kamiya K,Tsumoto K,Arakawa S,Shimizu S,Morita I,Yoshimura T,Akiyoshi K

更新日期：2010-12-01 00:00:00

abstract：:Proper design of fermentation facilities and equipment modification can control the risks associated with largescale production and purification of microbially produced cytotoxic agents and oncogenic viruses. The primary biohazard risks to operators and the environment are generation of aerosols and accidental spills....

journal_title：Biotechnology and bioengineering

authors： Flickinger MC,Sansone EB

更新日期：1984-08-01 00:00:00

abstract：:We report on a simple method to rapidly generate very large libraries of genes encoding mutant proteins without the use of DNA amplification, and the application of this methodology in the construction of synthetic immunoglobulin variable heavy (V(H)) and light (V(kappa)) libraries. Four high quality, chemically synth...

journal_title：Biotechnology and bioengineering

authors： Ge X,Mazor Y,Hunicke-Smith SP,Ellington AD,Georgiou G

更新日期：2010-06-15 00:00:00

abstract：:The misfolding of polypeptide chains and aggregation into the insoluble inclusion body state is a serious problem for biotechnology and biomedical research. Developing a rational strategy to control aggregation requires understanding the mechanism of polymerization. We investigated the in vitro aggregation of P22 tail...

journal_title：Biotechnology and bioengineering

authors： Speed MA,King J,Wang DI

更新日期：1997-05-20 00:00:00

abstract：:Ralstonia eutropha grows on and produces polyhydroxyalkanoates (PHAs) from fermentation acids. Acetic acid, one major organic acid from acidogenesis of organic wastes, has an inhibitory effect on the bacterium at slightly alkaline pH (6 g HAc/L at pH 8). The tolerance of R. eutropha to acetate, however, was increased ...

journal_title：Biotechnology and bioengineering

authors： Yu J,Wang J

更新日期：2001-06-20 00:00:00

abstract：:High methanol concentrations have a negative effect on the growth rate and the biomass yield of growth transients induced by methanol pulses in continuous cultures of Methylomonas L3. The physiological basis of this effect is investigated by measuring the effect of the methanol pulse on the cell energy charge (EC) and...

journal_title：Biotechnology and bioengineering

authors： Chu IM,Papoutsakis ET

更新日期：1987-01-01 00:00:00

abstract：:Two empirical models for the enzymatic hydrolysis of cellulose are used to analyze the same set of experimental data to determine if one model is superior to the other. Both models adequately describe the data. The parameters in both models may be correlated to the hydrolysis conditions of enzyme, substrate, and produ...

journal_title：Biotechnology and bioengineering

authors： Holtzapple MT,Caram HS,Humphrey AE

更新日期：1984-08-01 00:00:00

abstract：:A novel multivariable control strategy is developed for alcohol (ethanol and n-pentanol) concentrations in the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(HB-co-HV), a biodegradable polymer by Paracoccus denitrificans ATCC 1774. This controller, which is developed to control the mole fraction of P(HB...

journal_title：Biotechnology and bioengineering

authors： Chanprateep S,Abe N,Shimizu H,Yamane T,Shioya S

更新日期：2001-07-20 00:00:00

abstract：:Pyruvate production by Torulopsis glabrata was used as a model to study the mechanism of product inhibition and the strategy for enhancing pyruvate production. It was found that the concentration of cell growth and pyruvate deceased with the increase of NaCl and sorbitol concentrations. To enhance the osmotic stress r...

journal_title：Biotechnology and bioengineering

authors： Liu L,Xu Q,Li Y,Shi Z,Zhu Y,Du G,Chen J

更新日期：2007-07-01 00:00:00

abstract：:The inhibitory effect of ethanol on yeast growth and fermentation has been studied for the strain Saccharomyces cerevisiae ATCC No. 4126 under anaerobic batch conditions. The results obtained reveal that there is no striking difference between the response of growth and ethanol fermentation. Two kinetic models are als...

journal_title：Biotechnology and bioengineering

authors： Luong JH

更新日期：1985-03-01 00:00:00

abstract：:Two shock loads of a commercial detergent (I-150 mg chemical oxygen demand (COD)/L, fed for 56 h; II-300 mg COD/L fed for 222 h) were applied in a lab-scale Expanded Granular Sludge Blanket (EGSB) reactor, fed with 1,500 mg COD/L of ethanol. The impact of the surfactant was assessed in terms of granular sludge morphol...

journal_title：Biotechnology and bioengineering

authors： Costa JC,Abreu AA,Ferreira EC,Alves MM

更新日期：2007-09-01 00:00:00

abstract：:Product inhibition of biological production systems is a widely observed phenomenon with prominent implications for the design and ultimately the success of biotechnological processes. In order to investigate whether such effects could limit the maximal concentrations in the production of the beta-lactamase inhibitor ...

journal_title：Biotechnology and bioengineering

authors： Brethauer S,Held M,Panke S

更新日期：2008-06-15 00:00:00

abstract：:In our previous study [Hong Y, Brown DG (2009) Appl Environ Microbiol 75(8):2346-2353], the adenosine triphosphate (ATP) level of adhered bacteria was observed to be 2-5 times higher than that of planktonic bacteria. Consequently, the proton motive force (Delta p) of adhered bacteria was approximately 15% greater than...

journal_title：Biotechnology and bioengineering

authors： Hong Y,Brown DG

更新日期：2010-04-01 00:00:00

abstract：:Cartilage implants for potential in vivo use for joint repair or reconstructive surgery can be created in vitro by growing chondrocytes on biodegradable polymer scaffolds. Implants 1 cm in diameter by 0.176 cm thick were made using isolated calf chondrocytes and polyglucolic acid (PGA). By 6 weeks, the total amount of...

journal_title：Biotechnology and bioengineering

authors： Freed LE,Marquis JC,Langer R,Vunjak-Novakovic G,Emmanual J

更新日期：1994-03-25 00:00:00

abstract：:Ion-exchange (IEX) chromatography steps are widely applied in protein purification processes because of their high capacity, selectivity, robust operation, and well-understood principles. Optimization of IEX steps typically involves resin screening and selection of the pH and counterion concentrations of the load, was...

journal_title：Biotechnology and bioengineering

authors： Kelley BD,Switzer M,Bastek P,Kramarczyk JF,Molnar K,Yu T,Coffman J

更新日期：2008-08-01 00:00:00

abstract：:We have shown previously that a sequence-specific DNA-binding protein based on the Lac repressor protein can isolate pre-purified DNA efficiently from simple buffer solution but our attempts to purify plasmids directly from crude starting materials were disappointing with impractically low DNA yields. We have optimize...

journal_title：Biotechnology and bioengineering

authors： Darby RA,Forde GM,Slater NK,Hine AV

更新日期：2007-12-01 00:00:00

abstract：:An integrated approach is used to develop a rapid sampling strategy for the quantitative analysis of in vivo kinetic behavior based on measured concentrations of intracellular metabolites in Saccharomyces cerevisiae. Emphasis is laid on small sample sizes during sampling and analysis. Subsecond residence times are acc...

journal_title：Biotechnology and bioengineering

authors： Lange HC,Eman M,van Zuijlen G,Visser D,van Dam JC,Frank J,de Mattos MJ,Heijnen JJ

更新日期：2001-11-20 00:00:00

abstract：:Baculovirus has emerged as a promising vector for in vivo or ex vivo gene therapy. To date, the infectious titer and multiplicity of infection (MOI) based on the ability of baculovirus to infect insect cells are commonly adopted to indicate the virus dosage. However, the infectious titer and MOI do not reliably repres...

journal_title：Biotechnology and bioengineering

authors： Chan ZR,Lai CW,Lee HP,Chen HC,Hu YC

更新日期：2006-02-20 00:00:00

abstract：:The kinetics of the cellulase-catalyzed conversion of soluble cellulose into glucose have been studied over a range of substrate concentrations and temperatures, and at pH values ranging from 4.75 to 7.0. Lineweaver-Burk plots were linear and led to V = 6.2muM/s and K(m) = 13.1 mM at pH 5.8 and 25.0 degrees C. The pK ...

journal_title：Biotechnology and bioengineering

authors： Banerjee DK,Laidler KJ

更新日期：1983-10-01 00:00:00

abstract：:An in-situ, mid-infrared sensor was used to monitor the major analyte concentrations involved in the cultivation of Gluconacetobacter xylinus and the production of gluconacetan, a food-grade exopolysaccharide. To predict the analyte concentrations, three different sets of standard spectra were used to develop calibrat...

journal_title：Biotechnology and bioengineering

authors： Kornmann H,Rhiel M,Cannizzaro C,Marison I,von Stockar U

更新日期：2003-06-20 00:00:00

abstract：:Spores, although often considered metabolically inert, catalyze a variety of reactions. The use of spores instead of mycelium for bioconversions has several advantages. In this paper, we describe the difference in susceptibility of mycelium and spores against toxic substrates and products. A higher resistance of spore...

journal_title：Biotechnology and bioengineering

authors： Wolken WA,Tramper J,van der Werf MJ

更新日期：2002-12-20 00:00:00

abstract：:The removal of N-compounds in the sequencing batch airlift reactor (SBAR) containing granular sludge was studied under conditions of decreased dissolved oxygen (DO). A simulation model was developed to describe and evaluate the effects of several process conditions in the SBAR on N-removal performance. The model descr...

journal_title：Biotechnology and bioengineering

authors： Beun JJ,Heijnen JJ,van Loosdrecht MC

更新日期：2001-10-05 00:00:00

abstract：:Dilute aqueous solutions of polymers released by marine phytoplankton (microalgae) were shown to effectively reduce drag in capillary pipe flow. Tests were performed in a capillary turbulent flow viscometer which extruded small samples under high pressures. In all, 22 species were screened, and the products of one chl...

journal_title：Biotechnology and bioengineering

authors： Ramus J,Kenney BE,Shaughnessy EJ

更新日期：1989-01-25 00:00:00