Abstract:
:Proper design of fermentation facilities and equipment modification can control the risks associated with largescale production and purification of microbially produced cytotoxic agents and oncogenic viruses. The primary biohazard risks to operators and the environment are generation of aerosols and accidental spills. Fermentation and recovery facilities can be constructed to contain these agents by installing fermentation equipment within a HEPA-filter-exhausted biological barrier. Within this barrier system, large-scale processing that generates potentially hazaradous areosols (filtration, centrifugation of transformed cells or crystal slurries, and banding of viruses) should be isolated from other operations. Isolation of equipment is often required, with provision for both chemical and biological decontamination of process wastes. Failsafe fermentor over-pressure sensors, parallel exhaust gas filtration, welded transfer lines, and modified sampling systems for elimination of aerosols can be installed on most fermentation equipment. Aerosol and spill containment by proper equipment design, coupled with appropriate personnel protective equipment and medical monitoring, make possible safe production of experimental growth factors and viruses from large-scale culture of transformed mammalian cells and production of cytotoxic antitumor antibiotics.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Flickinger MC,Sansone EBdoi
10.1002/bit.260260808subject
Has Abstractpub_date
1984-08-01 00:00:00pages
860-70issue
8eissn
0006-3592issn
1097-0290journal_volume
26pub_type
杂志文章abstract::Microplates with integrated optical oxygen sensors are a new tool to study metabolic rates and enzyme activities. Precise measurements are possible only if oxygen exchange between the sample and the environment is known. In this study we quantify gas exchange in plastic microplates. Dissolved oxygen was detected using...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20348
更新日期:2005-05-05 00:00:00
abstract::Derivatives of Escherichia coli C were engineered to produce primarily succinate or malate in mineral salts media using simple fermentations (anaerobic stirred batch with pH control) without the addition of plasmids or foreign genes. This was done by a combination of gene deletions (genetic engineering) and metabolic ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21694
更新日期:2008-04-01 00:00:00
abstract::A two-stage bioreactor scheme was developed for the large-scale production of recombinant proteins using a genetically engineered baculovirus/insect cell system. The first bioreactor was employed for cell growth and the second for cell infection. Silkworm Bm5 cells were infected with a recombinant baculovirus, BmNPV/P...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260420313
更新日期:1993-07-01 00:00:00
abstract::A transient, two-culture model simulating methane production from biomass has been developed. The simulator, based partially on the work by Andrews and McCarty, is capable of calculating the hydrolysis products of several common organic materials, accommodating various substrate feeding modes, and simulating the trans...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260240903
更新日期:1982-09-01 00:00:00
abstract::A priori knowledge of the dissolved oxygen (O2) concentration profile within a hepatic hollow fiber (HF) bioreactor is important in developing an effective bioartificial liver assist device (BLAD). O2 provision is limiting within HF bioreactors and we hypothesize that supplementing a hepatic HF bioreactor's circulatin...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20673
更新日期:2006-02-05 00:00:00
abstract::We have shown previously that a sequence-specific DNA-binding protein based on the Lac repressor protein can isolate pre-purified DNA efficiently from simple buffer solution but our attempts to purify plasmids directly from crude starting materials were disappointing with impractically low DNA yields. We have optimize...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21492
更新日期:2007-12-01 00:00:00
abstract::Cell-division-cycle, temperature-sensitive mutants of Saccharomyces cerevisiae were investigated as a means of altering the morphological characteristics and subsequent physical properties of single-cell protein (SCP). Strain 4471, harboring mutation cdc 4, formed a visible complex mass at the nonpermissive temperatur...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260221007
更新日期:1980-10-01 00:00:00
abstract::The anaerobic oxidation of ammonium (anammox) uses nitrite as terminal electron acceptor. The nitrite can cause inhibition to the bacteria that catalyze the anammox reaction. The literature shows a great divergence on the levels of NO2 (-) causing inhibition. Moreover, the conditions influencing the resistance of anam...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25269
更新日期:2014-10-01 00:00:00
abstract::Efficient cofactor regeneration and reuse are highly desired for many important biotransformation applications. Here we show for the first time that cofactor NAD(H) covalently attached to micro particles, which can be easily recovered and reused, effectively mediated multistep reactions catalyzed by enzymes that were ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21584
更新日期:2008-02-15 00:00:00
abstract::The effect of different ammonium concentrations and glucosamine on baby hamster kidney (BHK)-21 cell cultures grown in continuously perfused double membrane bioreactors was investigated with respect to the final carbohydrate structures of a secretory recombinant glycoprotein. The human interleukin-2 (IL-2) mutant glyc...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1998-03-05 00:00:00
abstract::NS0, a nonsecreting mouse myeloma cell, is a major host line used for recombinant antibody production. These cells have a cholesterol-dependent phenotype and rely on an exogenous supply of cholesterol for their survival and growth. To better understand the physiology underlying cholesterol dependence, we compared NS0 ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20429
更新日期:2005-06-05 00:00:00
abstract::Cell-based tissue engineering is limited by the size of cell-containing constructs that can be successfully cultured in vitro. This limit is largely a result of the slow diffusion of molecules such as oxygen into the interior of three-dimensional scaffolds in static culture. Bioreactor culture has been shown to overco...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24497
更新日期:2012-09-01 00:00:00
abstract::The immobilization of dextransucrase in Ca-alginate beads relies on the close association between dextran polymer and dextransucrase. However, high amounts of dextran in the enzyme preparation drastically limit the specific activity of the immobilized enzyme (4 U/mL of alginate beads). Moreover, even in the absence of...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.1183
更新日期:2001-11-05 00:00:00
abstract::Melanoma is now the fifth most common type of cancer in North America. At present, there is no optimal treatment for this cancer. However, the lowering of the tyrosine level can inhibit the growth of melanoma. Unfortunately, this diet restriction cannot be humanly tolerated and causes vomiting, nausea, and severe body...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20084
更新日期:2004-06-30 00:00:00
abstract::A xenogeneic hollow fiber bioreactor utilizing collagen-entrapped dispersed hepatocytes has been developed as an extracorporeal bioartificial liver (BAL) for potential treatment of acute human fulminant hepatitis. Prolonged viability, enhanced liver-specific functions, and differentiated state have been observed in pr...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19961005)52:1<34::AID-BIT4
更新日期:1996-10-05 00:00:00
abstract::The glycerol fed-batch fermentation by Klebsiella pneumoniae CGMCC 1.6366 exhibited the sequential synthesis of products, including acetate, 1,3-propanediol (1,3-PD), 2,3-butanediol, ethanol, succinate, and lactate. The dominant flux distribution was shifted from acetate formation to 1,3-PD formation in early- exponen...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21830
更新日期:2008-08-01 00:00:00
abstract::Escherichia coli and Desulfovibrio desulfuricans reduce Tc(VII) (TcO(4)(-)) with formate or hydrogen as electron donors. The reaction is catalyzed by the hydrogenase component of the formate hydrogenlyase complex (FHL) of E. coli and is associated with a periplasmic hydrogenase activity in D. desulfuricans. Tc(VII) re...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::The major objectives for preparative protein chromatography are maximal loading and increased flow rate while maintaining defined resolution. Conventionally a series of chromatographic experiments are performed and the optimal conditions are selected according to the separation criteria. Computer-aided process design ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19961020)52:2<223::AID-BIT
更新日期:1996-10-20 00:00:00
abstract::Neural stem cells (NSCs) are undifferentiated, primitive cells with important potential applications including the replacement of neural tissue lost due to neurodegenerative diseases, including Parkinson's disease, as well as brain and spinal cord injuries, including stroke. We have developed methods to rapidly expand...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20246
更新日期:2004-11-05 00:00:00
abstract::Penicillinase (beta-lactamase I, EC 3.5.2.6) secreted by Bacillus cereus, strain 569/H, was covalently attached to aminoethyl cellulose via glutaraldehyde. The immobilized derivative shows increased thermostability and decreased susceptibility to conformational changes induced by certain substrates of penicillinase. T...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260210512
更新日期:1979-05-01 00:00:00
abstract::A protein loaded three-dimensional scaffold can be used for protein delivery and bone tissue regeneration. The main objective of this project was to develop recombinant human bone morphogenetic protein-2 (rhBMP-2) loaded poly(D,L-lactide-co-glycolide)/hydroxylapatite (PLGA/HAp) composite fibrous scaffolds through a pr...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21517
更新日期:2008-01-01 00:00:00
abstract::We study the role of nano-scale cues in controlling neuronal growth. We use photolithography to fabricate substrates with repeatable line-pattern ridges of nano-scale heights. We find that neuronal processes, which are of micron size, have strong interactions with ridges even as low as 10 nm. The interaction between t...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.24444
更新日期:2012-07-01 00:00:00
abstract::A cyclic fed-batch bioprocess is designed and a significant improvement of rice alpha-amylase productivity of recombinant Yarrowia lipolytica is illustrated. A bioprocess control strategy developed and reported here entails use of a genetically stable recombinant cloned for heterologous protein, use of optimized media...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19980805)59:3<379::aid-bit
更新日期:1998-08-05 00:00:00
abstract::Rate and yield expressions relating to biomass and xanthan formation and to nitrogen, glucose, and oxygen consumption were established for Xanthomonas campestris batch fermentations in a bubble column. Microbial growth was described by the logistic rate equation, characterized by a maximum specific growth rate mu(M) =...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260330404
更新日期:1989-01-20 00:00:00
abstract::Equilibrium isotherms for adsorption of bovine serum albumin (BSA) on a new adsorbent, a strongly basic crosslinked chitosan (Chitopearl 2503), which is hard and is not compressed by pressure in a column, have been presented and compared with diethylaminoethyl (DEAE) Sepharose Fast Flow (hard gel). In Chitopearl 2503,...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260431112
更新日期:1994-05-01 00:00:00
abstract::Nanoparticles (NPs) are able to deliver a variety of substances into eukaryotic cells. However, their usage is often hampered by a lack of specificity, leading to the undesired uptake of NPs by virtually all cell types. In contrast to this, yeast is known to be specifically taken up into immune cells after entering th...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.27226
更新日期:2020-03-01 00:00:00
abstract::The enzymatic hydrolysis of olive oil using Chromobacterium viscosum lipase B encapsulated in reversed micelles of dioctyl sodium sulfosuccinate (AOT) in isooctane was investigated in an ultrafiltration ceramic membrane reactor of tubular type, operating in a batch mode. Water concentration was found to be a critical ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260410802
更新日期:1993-04-05 00:00:00
abstract::This study investigates the scaling of photobioreactor productivity based on the growth of Nannochloropsis salina incorporating the effects of direct and diffuse light. The scaling and optimization of photobioreactor geometry was analyzed by determining the growth response of a small-scale system designed to represent...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23324
更新日期:2012-02-01 00:00:00
abstract::Recent environmental concerns have prompted a re-evaluation of conventional management strategies and refueled the search of innovative waste management practices. In this sense, the anaerobic digestion of both fat and the remaining complex organic matter present in dairy wastewaters is attractive, although the contin...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21095
更新日期:2007-02-01 00:00:00
abstract::Previous studies suggest that secretion of cloned proteins synthesized by recombinant Chinese hamster ovary (CHO) cells can be adenosine triphosphate (ATP) limited. Other research indicates that the presence of cloned Vitreoscilla hemoglobin (VHb) enhances ATP production in oxygen-limited Escherichia coli. To evaluate...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260441114
更新日期:1994-12-01 00:00:00