Human mesenchymal stem cell position within scaffolds influences cell fate during dynamic culture.


:Cell-based tissue engineering is limited by the size of cell-containing constructs that can be successfully cultured in vitro. This limit is largely a result of the slow diffusion of molecules such as oxygen into the interior of three-dimensional scaffolds in static culture. Bioreactor culture has been shown to overcome these limits. In this study we utilize a tubular perfusion system (TPS) bioreactor for the three-dimensional dynamic culture of human mesenchymal stem cells (hMSCs) in spherical alginate bead scaffolds. The goal of this study is to examine the effect of shear stress in the system and then quantify the proliferation and differentiation of hMSCs in different radial annuli of the scaffold. Shear stress was shown to have a temporal effect on hMSC osteoblastic differentiation with a strong correlation of shear stress, osteopontin, and bone morphogenic protein-2 occurring on day 21, and weaker correlation occurring at early timepoints. Further results revealed an approximate 2.5-fold increase in cell number in the inner annulus of TPS cultured constructs as compared to statically cultured constructs after 21 days. This result demonstrated a nutrient transfer limitation in static culture which can be mitigated by dynamic culture. A significant increase (P < 0.05) in mineralization in the inner and outer annuli of bioreactor cultured 4 mm scaffolds occurred on day 21 with 79 ± 29% and 53 ± 25% mineralization area, respectively, compared to 6 ± 4% and 19 ± 6% mineralization area, respectively, in inner and outer annuli of 4 mm statically cultured scaffolds. Surprising lower mineralization area was observed in 2 mm bioreactor cultured beads which had the highest levels of proliferation. These results may demonstrate a relationship between scaffold position and stem cell fate. In addition the decreased proliferation and matrix production in statically cultured scaffolds compared to bioreactor cultured constructs demonstrate the need for bioreactor systems and the effectiveness of the TPS bioreactor in promoting hMSC proliferation and differentiation in three-dimensional scaffolds.


Biotechnol Bioeng


Yeatts AB,Geibel EM,Fears FF,Fisher JP




Has Abstract


2012-09-01 00:00:00












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    pub_type: 杂志文章


    authors: Larroche C,Besson I,Gros JB

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Grimm LH,Kelly S,Hengstler J,Göbel A,Krull R,Hempel DC

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  • Development of waxy cassava with different Biological and physico-chemical characteristics of starches for industrial applications.

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Zhao SS,Dufour D,Sánchez T,Ceballos H,Zhang P

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  • Dissimilatory nitrate reduction by liquid membrane encapsulated cell-free extracts and whole cells of Micrococcus denitrificans.

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Markovits A

    更新日期:1978-10-01 00:00:00

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Coppella SJ,Dhurjati P

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yamane T,Kojima Y,Ichiryu T,Nagata M,Shimizu S

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    pub_type: 杂志文章


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    authors: Van Dedem G,Moo-Young M

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    journal_title:Biotechnology and bioengineering

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    authors: Kim JH,Lee JM,Jang WJ,Park HD,Kim YO,Kim CH,Kong IS

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    authors: Thomas B,Ohde D,Matthes S,Engelmann C,Bubenheim P,Terasaka K,Schlüter M,Liese A

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    authors: Sokol W

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    authors: Moussy Y

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    authors: Varma A,Palsson BO

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    authors: Seth G,Philp RJ,Denoya CD,McGrath K,Stutzman-Engwall KJ,Yap M,Hu WS

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    pub_type: 杂志文章


    authors: Laukevics JJ,Apsite AF,Viesturs US,Tengerdy RP

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: De Menezes TJ,Dos Santos CL,Azzini A

    更新日期:1983-04-01 00:00:00