Human mesenchymal stem cell position within scaffolds influences cell fate during dynamic culture.

abstract：:The bioconversion of octanoic acid into 2-heptanone by spores of Penicillium roquefortii is performed using a fed-batch technique with pH control by addition of the liquid substrate itself. The early stage of this process takes place with a high bioconversion rate and high yield. These values then decrease as a result...

journal_title：Biotechnology and bioengineering

authors： Larroche C,Besson I,Gros JB

更新日期：1994-09-05 00:00:00

abstract：:Glycolytic flux is increased and acetate production is reduced in Escherichia coli by the expression of heterologous NADH oxidase (NOX) from Streptococcus pneumoniae coupled with the deletion of the arcA gene, which encodes the ArcA regulatory protein. In this study, we examined the overproduction of a model recombina...

journal_title：Biotechnology and bioengineering

authors： Vemuri GN,Eiteman MA,Altman E

更新日期：2006-06-20 00:00:00

abstract：:We have produced Bioglass coatings for Orthopedic implants by using a novel coating technique, CoBlast. The two resultant surfaces, designated BG and hydroxyapatite (HA)/BG, were compared with their HA counterpart, OsteoZip in terms of osteoblastic cell attachment, adhesion, proliferation, differentiation, and growth ...

journal_title：Biotechnology and bioengineering

authors： Tan F,Naciri M,Al-Rubeai M

更新日期：2011-02-01 00:00:00

abstract：:Glycerol is considered as an ideal feedstock for producing bioplastics via bacterial fermentation due to its ubiquity, low price, and high degree of reduction substrate. In this work, we study the yield and cause of limitation in poly(3-hydroxybutyrate) (PHB) production from glycerol. Compared to glucose-based PHB pro...

journal_title：Biotechnology and bioengineering

authors： Tanadchangsaeng N,Yu J

更新日期：2012-11-01 00:00:00

abstract：:A novel multivariable control strategy is developed for alcohol (ethanol and n-pentanol) concentrations in the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(HB-co-HV), a biodegradable polymer by Paracoccus denitrificans ATCC 1774. This controller, which is developed to control the mole fraction of P(HB...

journal_title：Biotechnology and bioengineering

authors： Chanprateep S,Abe N,Shimizu H,Yamane T,Shioya S

更新日期：2001-07-20 00:00:00

abstract：:An automated system with semi on-line monitoring of glucose, lactic acid, protein, and optical density during lactic acid fermentations, is set up to study the dynamics of lactic acid bacteria. The analyzers for glucose, lactic acid, and protein are based on flow injection analysis (FIA). The system consists of a labo...

journal_title：Biotechnology and bioengineering

authors： Nielsen J,Nikolajsen K,Villadsen J

更新日期：1989-04-05 00:00:00

abstract：:Linear relations among net conversion rates of primary metabolites in fermentation processes can be determined by using the macroscopic or pathway balancing methods. It is shown that the process observability can be improved by incorporating both the abiotic proton balance and the pH control reagent addition rate meas...

journal_title：Biotechnology and bioengineering

authors： Siano SA

更新日期：1996-12-20 00:00:00

abstract：:In vivo kinetics of Saccharomyces cerevisiae are studied, in a time window of 150 s, by analyzing the response of O(2) and CO(2) in the fermentor off-gas after perturbation of chemostat cultures by metabolite pulses. Here, a new mathematical method is presented for the estimation of the in vivo oxygen uptake rate (OUR...

journal_title：Biotechnology and bioengineering

authors： Wu L,Lange HC,Van Gulik WM,Heijnen JJ

更新日期：2003-02-20 00:00:00

abstract：:This study conducted quantitative kinetic modeling and in situ and temporally resolved measurements of adsorption, desorption, and re-adsorption of a commercial endoglucanase in lignocellulosic suspensions. The study defined a cellulase adsorption and desorption competition parameter, a pseudo rate of binding and deso...

journal_title：Biotechnology and bioengineering

authors： Wang QQ,Zhu JY,Hunt CG,Zhan HY

更新日期：2012-08-01 00:00:00

abstract：:The naturally existing microbial hosts can rarely satisfy industrial requirements, thus there has always been an intense effort in strain engineering to meet the needs of these bioprocesses. Here, in this work, we want to prove the concept that engineering global transcription factor cAMP receptor protein (CRP) of Esc...

journal_title：Biotechnology and bioengineering

authors： Zhang H,Chong H,Ching CB,Jiang R

更新日期：2012-05-01 00:00:00

abstract：:The goal of drug delivery is to improve the safety and therapeutic efficacy of drugs. This review focuses on delivery platforms that are either derived from endogenous pathways, long-circulating biomolecules and cells or that piggyback onto long-circulating biomolecules and cells. The first class of such platforms is ...

journal_title：Biotechnology and bioengineering

authors： Yousefpour P,Chilkoti A

更新日期：2014-09-01 00:00:00

abstract：:Chondral and osteochondral lesions represent one of the most challenging problems in the orthopedic field, as these types of injuries lead to disability and worsened quality of life for patients and have an economic impact on the healthcare system. The aim of this in vivo study was to develop a new tissue engineering ...

journal_title：Biotechnology and bioengineering

authors： Tschon M,Brogini S,Parrilli A,Bertoldi S,Silini A,Parolini O,Faré S,Martini L,Veronesi F,Fini M,Giavaresi G

更新日期：2021-01-01 00:00:00

abstract：:The thermophilic fungus Sporotrichum cellulophilum was immobilized with nonwoven materials for cellulase production. The cellulose powder concentration in the medium was an important factor controlling cellulase production. When the cellulose powder concentration in the nonwoven materials was more than 4%, cellulase p...

journal_title：Biotechnology and bioengineering

authors： Tamada M,Kasai N,Kumakura M,Kaetsu I

更新日期：1986-08-01 00:00:00

abstract：:Fluorotelomer alcohols [FTOHs, F(CF(2))(n) CH(2)CH(2)OH, n = 4, 6, and 8] are emerging environmental contaminants. Biotransformation of FTOHs by mixed bacterial cultures has been reported; however, little is known about the microorganisms responsible for the biotransformation. Here we reported biotransformation of FTO...

journal_title：Biotechnology and bioengineering

authors： Kim MH,Wang N,McDonald T,Chu KH

更新日期：2012-12-01 00:00:00

abstract：:Aryl-alcohol oxidase (AAO) plays a fundamental role in the fungal ligninolytic secretome, acting as a supplier of H2 O2 . Despite its highly selective mechanism of action, the presence of this flavooxidase in different biotechnological settings has hitherto been hampered by the lack of appropriate heterologous express...

journal_title：Biotechnology and bioengineering

authors： Viña-Gonzalez J,Elbl K,Ponte X,Valero F,Alcalde M

更新日期：2018-07-01 00:00:00

abstract：:A second generation flow contraction device was developed and modeled which allows cells to be subjected to well-defined hydrodynamic forces. Studies were conducted with this system on wild-type Chinese Hamster Ovary cells (CHO-K1) and a strain of CHO cells which expresses the human Bcl-2 triangle gene (CHO-bcl-2). In...

journal_title：Biotechnology and bioengineering

authors： Mollet M,Godoy-Silva R,Berdugo C,Chalmers JJ

更新日期：2007-11-01 00:00:00

abstract：:The budding yeast Saccharomyces cerevisiae is equipped with G protein-coupled receptors (GPCR). Because the yeast GPCR signaling mechanism is partly similar to that of the mammalian system, S. cerevisiae can be used for a host of mammalian GPCR expression and ligand-mediated activation assays. However, currently avail...

journal_title：Biotechnology and bioengineering

authors： Fukutani Y,Ishii J,Kondo A,Ozawa T,Matsunami H,Yohda M

更新日期：2017-06-01 00:00:00

abstract：:N-linked glycan distribution affects important end-use characteristics such as the bioactivity and efficacy of many therapeutic proteins, (including monoclonal antibodies), in vivo. Yet, obtaining desired glycan distributions consistently during batch-to-batch production can be challenging for biopharmaceutical manufa...

journal_title：Biotechnology and bioengineering

authors： St Amand MM,Radhakrishnan D,Robinson AS,Ogunnaike BA

更新日期：2014-10-01 00:00:00

abstract：:Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important for protein stability, activity, and immunogenicity. However, understanding the roles and regulations of site-specific glycosylation events remains a significant challenge due to several technological limitations. These limi...

journal_title：Biotechnology and bioengineering

authors： Schoborg JA,Hershewe JM,Stark JC,Kightlinger W,Kath JE,Jaroentomeechai T,Natarajan A,DeLisa MP,Jewett MC

更新日期：2018-03-01 00:00:00

abstract：:The feasibility of continuous ester synthesis in a membrane bioreactor (MBR) by a recombinant cutinase from Fusarium solani pisi was investigated, using the optimal conditions previously attained by medium engineering. The objective was to analyze the MBR behavior as a differential or an integral reactor. The main com...

journal_title：Biotechnology and bioengineering

authors： Carvalho CM,Aires-Barros MR,Cabral JM

更新日期：2001-01-20 00:00:00

abstract：:Diffusion of phenol through a biofilm attached to activated carbon particles was investigated. The biofilm was grown on activated carbon particles in a draft-tube three-phase fluidized-bed bioreactor operating in a fed-batch mode. It was found that phenol did not adsorb on the biofilm and that the diffusion coefficien...

journal_title：Biotechnology and bioengineering

authors： Fan LS,Leyva-Ramos R,Wisecarver KD,Zehner BJ

更新日期：1990-02-05 00:00:00

abstract：:The production of soluble microbial products (SMP) in anaerobic systems was evaluated using chemostat reactors. Results from steady-state and tracer experiments with (14)C-glucose and (14)C-acetate showed that significant amounts of SMP were produced during the acidogenesis of glucose, but that SMP did not accumulate ...

journal_title：Biotechnology and bioengineering

authors： Noguera DR,Araki N,Rittmann BE

更新日期：1994-11-05 00:00:00

abstract：:Small interfering RNAs (siRNAs) are invaluable research tools for studying gene functions in mammalian cells. siRNAs are mainly produced by chemical synthesis or by enzymatic digestion of double-stranded RNA (dsRNA) produced in vitro. Recently, bacterial cells, engineered with ectopic plant viral siRNA binding protein...

journal_title：Biotechnology and bioengineering

authors： Kaur G,Cheung HC,Xu W,Wong JV,Chan FF,Li Y,McReynolds L,Huang L

更新日期：2018-09-01 00:00:00

abstract：:The key to achieving successful, reproducible results following the introduction of beneficial microbes into soil relies on the survival rate of the inoculated bacteria in a heterogeneous soil environment and hence an improved encapsulation method was developed. Owing to the constraints associated with the inoculum fo...

journal_title：Biotechnology and bioengineering

authors： Young CC,Rekha PD,Lai WA,Arun AB

更新日期：2006-09-05 00:00:00

abstract：:Nitrous oxide (N2 O), a potent greenhouse gas, is reduced to N2 gas by N2 O-reducing bacteria (N2 ORB), a process which represents an N2 O sink in natural and engineered ecosystems. The N2 O sink activity by N2 ORB depends on temperature and O2 exposure, yet the specifics are not yet understood. This study explores th...

journal_title：Biotechnology and bioengineering

authors： Zhou Y,Suenaga T,Qi C,Riya S,Hosomi M,Terada A

更新日期：2020-12-11 00:00:00

abstract：:Substrate transfer rates from organic to aqueous phases were measured in the presence and absence of biocatalyst in the reaction medium, using modified Lewis cells. These measurements, in combination with intrinsic aqueous phase biocatalytic reaction kinetics, were used to confirm that benzyl acetate hydrolysis by pig...

journal_title：Biotechnology and bioengineering

authors： Woodley JM,Brazier AJ,Lilly MD

更新日期：1991-01-20 00:00:00

abstract：:In this article, we consider the impact on downstream process design resulting from the use of metabolically engineered yeast strains. We address the issue of how manipulation of cell wall permeability can improve the release and subsequent recovery of heterologous products produced in yeast. ...

journal_title：Biotechnology and bioengineering

authors： Ferrer P,Diers I,Asenjo JA,Andrews BA

更新日期：1998-04-20 00:00:00

abstract：:To build complex genetic networks with predictable behaviors, synthetic biologists use libraries of modular parts that can be characterized in isolation and assembled together to create programmable higher-order functions. Characterization experiments and computational models for gene regulatory parts operating in iso...

journal_title：Biotechnology and bioengineering

authors： Westbrook A,Tang X,Marshall R,Maxwell CS,Chappell J,Agrawal DK,Dunlop MJ,Noireaux V,Beisel CL,Lucks J,Franco E

更新日期：2019-05-01 00:00:00

abstract：:Blood vessel development is critical for the continued growth and progression of solid tumors and, therefore, makes an attractive target for improving cancer therapy. Indeed, vascular-targeted therapies have been extensively explored but they have shown minimal efficacy as monotherapies. Combretastatin A4 (CA-4) is a ...

journal_title：Biotechnology and bioengineering

authors： Liu Y,Kim YJ,Siriwon N,Rohrs JA,Yu Z,Wanga P

更新日期：2018-06-01 00:00:00

abstract：:A key point of protein stability engineering is to identify specific target residues whose mutations can stabilize the protein structure without negatively affecting the function or activity of the protein. Here, we propose a method called RiSLnet (Rapid identification of Smart mutant Library using residue network) to...

journal_title：Biotechnology and bioengineering

authors： Upadhyay R,Kim JY,Hong EY,Lee SG,Seo JH,Kim BG

更新日期：2019-02-01 00:00:00