Abstract:
:A biodevice involving thiolated ssDNA and engineered cytochrome b5 linked through a cis-platine bridge is described. This original nanostructure is associated to a supported membrane through a floating anchor, thus constituting a dynamic bidimensionnal DNA capture device contrasting with the constrained geometry of currently available DNA chips. Characterization by optical spectroscopy, surface plasmon resonance and gel electrophoresis demonstrated that an unique molecular supra-assembly featuring specific DNA recognition capability has been obtained. This device is characterized by the reversibility of its assembly, self-organization and fluidity properties and is of interest as a prototype to design new generations of DNA chip biosensors.
journal_name
Biotechnol Bioengjournal_title
Biotechnology and bioengineeringauthors
Boireau W,Bombard S,Sari MA,Pompon Ddoi
10.1002/bit.10111subject
Has Abstractpub_date
2002-01-20 00:00:00pages
225-31issue
2eissn
0006-3592issn
1097-0290pii
10.1002/bit.10111journal_volume
77pub_type
杂志文章abstract::Multiparameter flow cytometric techniques developed in our laboratories have been used for the "at-line" study of fed-batch bacterial fermentations. These fermentations were done at two scales, production (20 m(3)) and bench (5 x 10(-3) m(3)). In addition, at the bench scale, experiments were undertaken where the diff...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:
更新日期:2000-11-20 00:00:00
abstract::The release kinetics of beta-galactosidase protein have been determined during small-scale ultrasonication of E. coli cells. Among several studied parameters, ionic strength and cell concentration have the least influence on the rate of protein recovery, whereas sample volume and acoustic power dramatically affect the...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(sici)1097-0290(19980605)58:5<536::aid-bit
更新日期:1998-06-05 00:00:00
abstract::Membrane chromatography (MC) systems are finding increasing use in downstream processing trains for therapeutic proteins due to the unique mass-transfer characteristics they provide. As a result, there is increased need for model-based methods to scale-up MC units using data collected on a scaled-down unit. Here, a st...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25217
更新日期:2014-08-01 00:00:00
abstract::The diffusivities of glucose and ethanol in cell-free and cell-occupied membranes of calcium alginate were measured in a diffusion cell. The lag time analysis was used. Diffusivities decreased with increasing alginate concentration and were comparable with those in water for a 2% alginate membrane. Glucose and ethanol...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260280609
更新日期:1986-06-01 00:00:00
abstract::Potentially damaging reactive oxygen species (ROS) are involved in a number of pathways ranging from signal transduction to apoptosis. Cells have adapted this alteration in redox status into a complex regulatory mechanism. ROS are specifically able to induce the expression of a multitude of genes. We constructed and c...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20389
更新日期:2005-03-05 00:00:00
abstract::Protein-protein interactions were measured for ovalbumin and for lysozyme in aqueous salt solutions. Protein-protein interactions are correlated with a proposed potential of mean force equal to the free energy to desolvate the protein surface that is made inaccessible to the solvent due to the protein-protein interact...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10342
更新日期:2002-08-20 00:00:00
abstract::A submerged anaerobic dynamic membrane bioreactor (AnDMBR) was operated for treatment of concentrated wastewater. The dynamic membrane (DM) or cake layer was characterized on its physicochemical and biological composition and the role of the DM layer in treatment and filtration performances was assessed. The results s...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25841
更新日期:2016-04-01 00:00:00
abstract::Production of cellulase using solid culture systems of Trichoderma reesei QM9414 and Sporotrichum cellulophilum on wheat bran was studied. By using moisture-controlled solid culture equipment, the effect of water content of wheat bran on cell growth and cellulase production was investigated. Cellular biomass grown on ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260271008
更新日期:1985-10-01 00:00:00
abstract::The Chinese hamster ovary (CHO) cell line producing interferon-gamma (IFN-gamma) exhibits a 2-fold increase in specific productivity when grown at 32 degrees C compared to 37 degrees C. Low temperature also causes growth arrest, meaning that the cell density is significantly lower at 32 degrees C, nutrients are consum...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10861
更新日期:2004-01-20 00:00:00
abstract::The implementation of control algorithms to batch bioreactors is often complicated by variations in process dynamics that occur during the course of fermentation. Such a wide operating range often renders the performance of fixed gain proportional-integral-differential (PID) controllers unsatisfactory. In this work, d...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260320415
更新日期:1988-08-05 00:00:00
abstract::Contactless simultaneous positioning of micrometer-sized particles in suspension (e.g., copolymer beads, living cells, silicon microparts) can be performed using ultrasound. Current devices are capable of collecting particles into planes or lines by exciting a resonance in the fluid by means of a piezoelectric transdu...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21315
更新日期:2007-08-01 00:00:00
abstract::Cells usually spread on a synthetic substrate through bonds between receptors and chemical groups on the substrate (ligands). Therefore, it is valuable to study the effects of the average number density of these chemical groups and the average distance between them to model and predict the cell behavior. Poly(ethylene...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22889
更新日期:2011-02-01 00:00:00
abstract::In this study, a useful method was developed to fabricate array patterns of microparticles not on electrode surfaces, but on arbitrary surfaces, using negative-dielectrophoresis (n-DEP). First, electrodes were designed and electric field simulations were performed to manipulate microparticles toward target areas. Base...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22445
更新日期:2009-11-01 00:00:00
abstract::Tissue function is comprised of a complex interplay between biological and physicochemical rate processes. The design of bioreactors for tissue engineering must account for these processes simultaneously in order to obtain a bioreactor that provides a uniform environment for tissue growth and development. In the prese...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960605)50:5<479::AID-BIT
更新日期:1996-06-05 00:00:00
abstract::Fluorescence in situ hybridization (FISH) has been extensively used in the past decades for the detection and localization of microorganisms. However, a mechanistic approach of the whole FISH process is still missing, and the main limiting steps for the hybridization to occur remain unclear. In here, FISH is approache...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章,评审
doi:10.1002/bit.27462
更新日期:2020-10-01 00:00:00
abstract::The effects of external calcium concentrations on biosynthesis of ginsenoside Rb1 and several calcium signal sensors were quantitatively investigated in suspension cultures of Panax notoginseng cells. It was observed that the synthesis of intracellular ginsenoside Rb1 in 3-day incubation was dependent on the medium Ca...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.20386
更新日期:2005-02-20 00:00:00
abstract::The effects of ZnCl2 additions on a mercuric reductase, merA, ammonia monooxygenase, amoA, and hydroxylamine (NH2OH) oxidoreductase, hao, gene expression were examined in continuously cultured Nitrosomonas europaea cells. The reactor was operated for 85 days with a 6.9 d hydraulic retention time and with four successi...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22069
更新日期:2009-02-01 00:00:00
abstract::The adsorption of cellulase on cellulose and a lignacious residue was examined by using cellulase from Trichoderma reesei, hardwood pretreated by dilute sulfuric acid under high pressure, and a lignacious residue prepared by a complete enzymatic hydrolysis of the pretreated wood. A significant amount of cellulase was ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260360503
更新日期:1990-08-20 00:00:00
abstract::A simple approach was developed to determine the half-saturation coefficient for dissolved oxygen (K(DO)) for three bacteria by maintaining a constant oxygen concentration in continuous culture, and employing a dynamic method to obtain the specific growth rate (mu) for each species. Measurement of mu at selected disso...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.260250209
更新日期:1983-02-01 00:00:00
abstract::Preparative-scale purification of plasmid DNA has been attempted by diverse methods, including precipitation with solvents, salts, and detergents and chromatography with ion-exchange, reversed-phase, and size-exclusion columns. Chromatographic methods such as hydrophobic interaction chromatography (HIC), reversed phas...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.10335
更新日期:2002-09-30 00:00:00
abstract::Somatic embryogenesis is among the most promising means of large scale plant micropropagation. The development of somatic embryos is characterized by their morphological changes. Embryos in culture usually exhibit high heterogeneity and abnormality. As conventional microscopic observation is laborious and subjective, ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0290(19960405)50:1<65::AID-BIT8
更新日期:1996-04-05 00:00:00
abstract::A desirable feature of many therapeutic glycoprotein production processes is to maximize the final sialic acid content. In this study, the effect of applying a novel chemical analog of the sialic acid precursor N-acetylmannosamine (ManNAc) on the sialic acid content of cellular proteins and a model recombinant glycopr...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26291
更新日期:2017-08-01 00:00:00
abstract::The effect of trehalose (0.5 M) on the thermal stability of cutinase in the alkaline pH range was studied. The thermal unfolding induced by increasing temperature was analyzed in the absence and in the presence of trehalose according to a two-state model (which assumes that only the folded and unfolded states of cutin...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/1097-0290(20001220)70:6<699::aid-bit13>3.0
更新日期:2000-12-20 00:00:00
abstract::We have produced Bioglass coatings for Orthopedic implants by using a novel coating technique, CoBlast. The two resultant surfaces, designated BG and hydroxyapatite (HA)/BG, were compared with their HA counterpart, OsteoZip in terms of osteoblastic cell attachment, adhesion, proliferation, differentiation, and growth ...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22955
更新日期:2011-02-01 00:00:00
abstract::Abnormal airway mucus presents a significant challenge for inhalational drug delivery. Recognizing the thick and tenacious airway mucus seen in the cystic fibrosis (CF) patients as a critical barrier to effective drug delivery, both into the mucus layer itself as well as across that layer to the underlying airway epit...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23046
更新日期:2011-06-01 00:00:00
abstract::The functional analysis of plant cells at the cellular and subcellular levels requires novel technologies for the directed manipulation of individual cells. In this report, we demonstrate the use of an infrared (1,064 nm) picosecond laser for the perforation of tobacco cell protoplasts. A single pulse was sufficient t...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.21549
更新日期:2008-01-01 00:00:00
abstract::To gain insight on the fate of arsenic (As) from drinking water treatment residuals in landfills, the mobilization of arsenate adsorbed onto granular ferric hydroxide (GFH) was studied in continuous anaerobic columns fed with a synthetic landfill leachate. The release of As was compared in biologically active and abio...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.22021
更新日期:2008-12-15 00:00:00
abstract::The effect of ligand density on anion-exchange membrane chromatography (AEXmc) for the purification of recombinant baculoviruses (rBVs), potential viral vectors in clinical applications, is studied by surface plasmon resonance on customized AEX surfaces and gradient elution experiments on Sartobind D membrane prototyp...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.23058
更新日期:2011-06-01 00:00:00
abstract::Lutein and zeaxanthin are dietary carotenoids reported to be protective against age-related macular degeneration. Recently, the green alga Chlamydomonas reinhardtii has received attention as a photosynthetic cell factory, but the potential of this alga for carotenoid production has not yet been evaluated. In this stud...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.26499
更新日期:2018-03-01 00:00:00
abstract::Non-destructive label-free continuous monitoring of in vitro tissue culture is an unmet demand in tissue engineering. Noting that different compositions of cartilage lead to different electrical tissue properties, we propose a new method to measure the electrical conductivity and its anisotropy during in vitro chondro...
journal_title:Biotechnology and bioengineering
pub_type: 杂志文章
doi:10.1002/bit.25350
更新日期:2015-02-01 00:00:00