Expression of merA, amoA and hao in continuously cultured Nitrosomonas europaea cells exposed to zinc chloride additions.


:The effects of ZnCl2 additions on a mercuric reductase, merA, ammonia monooxygenase, amoA, and hydroxylamine (NH2OH) oxidoreductase, hao, gene expression were examined in continuously cultured Nitrosomonas europaea cells. The reactor was operated for 85 days with a 6.9 d hydraulic retention time and with four successive additions of ZnCl2 achieving maximum concentrations from 3 to 90 microM Zn2+. Continuously cultured N. europaea cells were more resistant to Zn2+ inhibition than previously examined batch cultured cells due to the presence of Mg2+ in the growth media, suggesting that Zn2+ enters the cell through Mg2+ import channels. The maximum merA up-regulation was 45-fold and expression increased with increases in Zn2+ concentration and decreased as Zn2+ concentrations decreased. Although Zn2+ irreversibly inactivated ammonia oxidation in N. europaea, the addition of either 600 microM CuSO4 or 2250 microM MgSO4 protected N. europaea from ZnCl2 inhibition, indicating a competition between Zn2+ and Cu2+/Mg2+ for uptake and/or AMO active sites. Since ZnCl2 inhibition is irreversible and amoA was up-regulated at 30 and 90 microM additions, it is hypothesized that de novo synthesis of the AMO enzyme is needed to overcome inhibition. The up-regulation of merA during exposure to non-inhibitory Zn2+ levels indicates that merA is an excellent early warning signal for Zn2+ inhibition.


Biotechnol Bioeng


Radniecki TS,Semprini L,Dolan ME




Has Abstract


2009-02-01 00:00:00












  • Comparison of microbial community composition and activity in sulfate-reducing batch systems remediating mine drainage.

    abstract::Five microbial inocula were evaluated in batch tests for the ability to remediate mine drainage (MD). Dairy manure (DM), anaerobic digester sludge, substrate from the Luttrell (LUTR) and Peerless Jenny King (PJK) sulfate-reducing permeable reactive zones (SR-PRZs) and material from an MD-treatment column that had been...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Pereyra LP,Hiibel SR,Pruden A,Reardon KF

    更新日期:2008-11-01 00:00:00

  • Mannitol-guided delivery of Ciprofloxacin in artificial cystic fibrosis mucus model.

    abstract::Abnormal airway mucus presents a significant challenge for inhalational drug delivery. Recognizing the thick and tenacious airway mucus seen in the cystic fibrosis (CF) patients as a critical barrier to effective drug delivery, both into the mucus layer itself as well as across that layer to the underlying airway epit...

    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Yang Y,Tsifansky MD,Shin S,Lin Q,Yeo Y

    更新日期:2011-06-01 00:00:00

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Stieglitz B,Defelice CP

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  • Mechanical load increase-induced changes in cytoskeletal structure and cellular barrier function in human cerebral endothelial cells.

    abstract::Globally, approximately a billion patients are estimated to suffer from neurological disorders. Although there are many therapeutic candidates for the central nervous system, treatment of brain disorders is restricted by the blood-brain barrier (BBB), which is a highly selective membrane that protects the brain from e...

    journal_title:Biotechnology and bioengineering

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    authors: Kim D,Kwon S

    更新日期:2018-10-01 00:00:00

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    journal_title:Biotechnology and bioengineering

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    authors: Martínez VS,Dietmair S,Quek LE,Hodson MP,Gray P,Nielsen LK

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    pub_type: 杂志文章


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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Barnabé N,Butler M

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    pub_type: 杂志文章


    authors: Alpkvist E,Picioreanu C,van Loosdrecht MC,Heyden A

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    authors: Hao OJ,Richard MG,Jenkins D,Blanch HW

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    abstract::An approach is presented for the stable covalent immobilization of proteins with a high retention of biological activity. First, chemical modification studies were used to establish enzyme structural and functional properties relevant to the covalent immobilization of an enzyme to agarose based supports. Heparinase wa...

    journal_title:Biotechnology and bioengineering

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    authors: Nishikawa M

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    authors: van de Vondervoort PJ,Poulsen BR,Ruijter GJ,Schuleit T,Visser J,Iversen JJ

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    journal_title:Biotechnology and bioengineering

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    authors: Guisasola A,Pijuan M,Baeza JA,Carrera J,Casas C,Lafuente J

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Pilon L,Barbe MC,Desrochers M,Jurasek L,Neumann PJ

    更新日期:1982-09-01 00:00:00

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    journal_title:Biotechnology and bioengineering

    pub_type: 杂志文章


    authors: Liu P,Ryczko M,Xie X,Baardsnes J,Lord-Dufour S,Duroche Y,Hicks EA,Taiyab A,Sheardown H,Quaggin SE,Jin J

    更新日期:2021-01-01 00:00:00